scholarly journals Confocal laser scanning microscopic evaluation of depth of penetration and Sealer/Dentin interface between endodontic sealers - An in-vitro study

2021 ◽  
Vol 8 (2) ◽  
pp. 62-67
Author(s):  
Lakshmi Balaji ◽  
Sukanya Kanaga Raj ◽  
Preethi V ◽  
Kandaswamy Deivanayagam
Keyword(s):  
Root Canal ◽  
Maximum Depth ◽  
Depth Of Penetration ◽  
Group Iii ◽  
Group I ◽  
Microscopic Evaluation ◽  
Ah Plus ◽  
Group Ii ◽  
Endodontic Sealers ◽  
One Way Anova

An endodontic sealer contributes in obtaining an appropriate seal for the root canal system by establishing an association between the root canal walls and coronal restoration thereby promoting healing. This present research intended to compare the penetrating capacity and to determine sealer/dentin interface among various endodontic sealers. Horizontally sectioned tooth samples were obtained at 3 and 5mm and randomly divided into three groups: Group I - Chemically cured (AH Plus) sealer; Group II - Dual cured (EndoREZ) sealer; Group III – BioCeramic Nano sealer (iRoot SP). All three groups were coated with their respective sealers and obturated using cold lateral compaction technique. The samples were observed using Confocal Microscope for penetrating capacity evaluation and reviewing of sealer/dentin junction at 3 and 5mm depth. The results were statistically analyzed using one-way ANOVA and Tukey's Post-Hoc method. On depth of penetration evaluation, one-way ANOVA analysis showed Group II exhibited maximum depth of penetration in contrast to Group III and Group I. Group I showed the least average depth of penetration among the other two groups at both 3 and 5mm. On sealer/dentin interface evaluation at 3mm, Group II showed increased interface in comparison with to Group III and Group I. No statistical significance evident among the groups at 5mm. EndoREZ showed maximum depth of penetration in comparison with iRoot SP and AH Plus sealer at both 3 and 5mm. EndoREZ also showed significant sealer/dentin interface at the level of 3mm when compared to other sealers used.

An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser

2013 ◽  
Vol 14 (2) ◽  
pp. 183-187 ◽  
Author(s):  
G Anil Kumar ◽  
Maneesha Das ◽  
Sindhu Ramesh ◽  
Surendranath Garapati
Keyword(s):  
Bond Strength ◽  
Nd:Yag Laser ◽  
Root Canal ◽  
Smear Layer ◽  
Control Group ◽  
Microtensile Bond Strength ◽  
Group Iii ◽  
Group I ◽  
Ah Plus

ABSTRACT Background Smear layer is a negative factor which prevents adhesion of the filling material to the dentinal walls. Recent advances in dental research have incorporated lasers as a potential adjunct in root canal treatment by removing the smear layer before filling the root canal system, enhancing the adhesion of sealers to dentin and improving the sealing ability. Aim To evaluate the microtensile bond strength of AH-Plus resin-based sealer to dentin after treatment with 980 nm diode and 1,064 nm neodymium-doped:yttrium aluminum garnet (Nd:YAG) laser in vitro. Materials and methods Thirty specimens prepared for three groups namely group I (control), group II (980 nm diode–lased specimens) and group III (Nd:YAG–lased specimens). One tooth from each group was observed under scanning electron microscope for evaluation of intracanal root dentin morphology. Remaining specimens were used for making microsections by hard tissue microtome. Specimens for groups II and III were lased with 980 nm diode and 1,064 nm Nd:YAG laser. AH Plus sealer was applied onto specimens and mounted onto Instron universal testing machine for microtensile bond strength testing. Results were subjected to statistical analysis using one-way analysis of variance (ANOVA) and Tukey's test. Results Group III Nd:YAG had maximum mean microtensile bond strength values (11.558 ± 0.869), followed by group II diode (9.073 ± 0.468) and group I control (6.05 ± 0.036). Statistically significant differences were seen among all the groups. SEM analysis shows removal of smear layer in both groups II and III. Conclusion Both Nd:YAG and diode laser were more effective than control group in improving the microtensile bond strength of AH Plus sealer to dentin. Clinical significance Lasers have the potential to increase the adhesiveness of root canal sealer to dentin surface, thereby improving the quality of root canal obturation. How to cite this article Maneesha D, Anil KG, Sindhu R, Surendranath G, Deepak S. An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser. J Contemp Dent Pract 2013;14(2): 183-187.

scholarly journals Pengaruh Suhu Terhadap Penyerapan Air Dan Kelarutan Resin Komposit

2017 ◽  
Vol 6 (2) ◽  
pp. 59
Author(s):  
Kholidina Imanda Harahap ◽  
Rusfian Rusfian ◽  
Affan Ali Al-Harits
Keyword(s):  
Water Sorption ◽  
Composite Resin ◽  
Testing Procedure ◽  
Effect Of Temperature ◽  
Temperature Changes ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Dental Restorative ◽  
One Way Anova

The existence of composite resin as dental restorative materials in the mouth will always be exposed to temperature changes from food or drink. The purpose of this study was to evaluate the effect of temperature on water absorption and solubility of the composite resin. Thirty samples made from nanofiller composite resin with a diameter of 15 mm and a thickness of 1 mm. The samples were divided into three test groups (n=10) that is immersed at 50 (I), 37 (II) and 5 °C (III). The testing procedure of water sorption and solubility of the composite resin made according to the ISO 4049: 2000 specifications. Data will be analyzed by one-way ANOVA and Least Significance Data test (p=0,05). The results showed there is significant differences of the water sorption (p=0,045)  and solubility (p=0,018). The highest water sorption is group I (0,0057±0,007µg/mm3) than group II (0,0028±0,0013 µg/mm) and group III (0,0005±0,0006 µg/mm). While the heighest solubility values   are in group I (0,003 ± 0,0027 µg/ mm), than group II (0,002 ± 0,0013 µg/mm) and group III (0,001 ± 0,00 µg/mm). It can be concluded that temperature could increase water sorption and solubility of composite resin.

scholarly journals Comparative antimicrobial efficacy of selected root canal irrigants on commonly isolated microorganisms in endodontic infection

2017 ◽  
Vol 11 (01) ◽  
pp. 012-016 ◽  
Author(s):  
Sandeep Dubey ◽  
Suparna Ganguly Saha ◽  
Balakrishnan Rajkumar ◽  
Tapan Kumar Dhole
Keyword(s):  
Normal Saline ◽  
Root Canal ◽  
Antibacterial Effect ◽  
Group Iv ◽  
Antimicrobial Efficacy ◽  
Group Iii ◽  
Root Canal Irrigants ◽  
Group I ◽  
Group Ii ◽  
Root Canal Irrigant

ABSTRACT Objective: This study aims to evaluate and compare the antimicrobial efficacy of three selected root canal irrigants (BioPure MTAD, metronidazole, aztreonam) against microbes commonly isolated from polymicrobial microbiota of root canal infection. Materials and Methods: This study was designed with four experimental groups (Group I - Bacteroides fragilis, Group II - Propionibacterium acnes, Group III - Enterococcus faecalis, Group IV - Candida albicans) based on the microbes selected for the study. Group I and Group II bacteria were used to compare and evaluate antimicrobial effect of BioPure MTAD, metronidazole, aztreonam, and normal saline. Group III and Group IV bacteria were used to compare and evaluate antimicrobial efficacy of BioPure MTAD, aztreonam, and normal saline. Normal saline was used as a control irrigant in this study. Agar disc diffusion method was applied to assess and compare the antimicrobial action of selected irrigants. Results: Metronidazole was found to be the most effective root canal irrigant against B. fragilis and P. acnes among the tested irrigants. Mean zone of inhibition against E. faecalis has been shown to be maximum by BioPure MTAD, followed by aztreonam. Antifungal effect against C. albicans was only shown by BioPure MTAD. Conclusions: Overall, BioPure MTAD is the most effective root canal irrigant as it has shown an antibacterial effect against all the tested microorganisms. However, metronidazole showed maximum antibacterial effect against obligate anaerobes. Aztreonam also showed an antibacterial effect in the present study, raising its possibility to be used as a root canal irrigant in the future.

scholarly journals Effects of Acute and Chronic Exposure to 900 Mhz Electromagnetic Field on the Rat Liver Microarchitecture

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1585
Author(s):  
Elvan Şahin ◽  
Derya Güzel ◽  
Şadiye Açıkgöz ◽  
Nihal Tufan
Keyword(s):  
Rat Liver ◽  
Chronic Exposure ◽  
Exposed Group ◽  
The Body ◽  
Exposure Group ◽  
Group Iii ◽  
Group I ◽  
Microscopic Evaluation ◽  
Group Ii ◽  
Acute And Chronic Exposure

Technological devices such as mobile phones, wi-fi and bluetooth applications used widely in daily life emit low-dose electromagnetic fields (EMFs). EMFs influence metabolic processes in the body and exert various harmful biological effects on cells. This study aims to evaluate acute and chronic effects of 900 MHz EMF exposure on the microscopic structure of rat liver. Twenty-four adult Wistar albino rats were randomly divided into sham-control (Group I), acutely EMF-exposed (Group II) and chronically EMF-exposed (Group III) groups (n = 8 in each). The Rats of Group II were exposed to 900 MHz EMF using a microwave test transmitter during 24 h continuously just for one day. Group III-rats were exposed to 900 MHz EMF for 60 minutes a day for 30 days. Sham-controls were not applied EMF. After the rats were sacrificed under the anesthesia, their livers were removed and processed for light microscopic evaluation. Liver sections stained with histochemical dyes (H&E, PAS and Masson’s trichrome) displayed many histopathological alterations in both of the EMF-exposed groups, including foci of necrosis, inflammation, excessive vacuolar degenerations and apoptosis in hepatocytes, apparent vascular expansions and haemorrhage. Additionally, mononuclear cell infiltrations, biliary hyperplasia, fibrosis in periportal and centrilobular areas and decreased Kupffer cell population were determined in the chronic EMF exposure group. In contrast, the amount of the Kupffer cells were much more in the acute exposure group. Our findings suggested that both acute and chronic exposure to 900 MHz EMFs can lead to hepatic injury in rats.

scholarly journals Effects of incorporation of silver and titanium nanoparticles on feldspathic ceramic toughness

2019 ◽  
Vol 13 (2) ◽  
pp. 98-102
Author(s):  
Vasudevan Karthikeyan ◽  
Naveen Gopi Chander ◽  
Jetti Ramesh Reddy ◽  
Balasubramanium Muthukumar
Keyword(s):  
Fracture Toughness ◽  
Silver Nanoparticles ◽  
Mean Values ◽  
Group Iii ◽  
Group I ◽  
Feldspathic Porcelain ◽  
Titanium Nanoparticles ◽  
Group Ii ◽  
Post Hoc ◽  
One Way Anova

Background. Porcelain is brittle and in many situations it requires replacement in fractured or chipped restorations. The prospects exist in improving the strength of feldspathic porcelain to widen its applications. This study evaluated the fracture toughness of feldspathic porcelain after incorporation of silver and titanium nanoparticles at varying concentrations (0.5 wt%, 1 wt%, 1.5 wt% and 2 wt%). Methods. Test specimen dimensions were standardized to ASTM C1421–16 standards and a three-point flexure test was carried out to evaluate fracture toughness. A total of 330 samples were fabricated and broadly divided into three groups. Group I (titanium nanoparticles) and group II (silver nanoparticles) were further subdivided into 5 groups (0 wt%, 0.5 wt%, 1 wt%, 1.5 wt% and 2 wt%) for fracture toughness analysis, with each group consisting of 30 samples. Group III contained the superior concentration of both titanium and silver nanoparticles. The fracture toughness (KIC) was calculated using indentation fracture method and microstructure observations were made using scanning electron microscopy. The KIC values were compared and evaluated using one-way ANOVA. Results. Data were analyzed using one-way ANOVA and Tukey’s HSD post hoc test multiple comparisons. The mean values of group I, group II and group III were 1.949 MPa.m1/2, 2.002 MPa.m1/2 and 1.330 MPa.m1/2 , respectively. Conclusion. The results revealed that the samples reinforced with titanium and silver nanoparticles showed significant increases in fracture toughness. The blending of superior concentration of both titanium and silver nanoparticles decreased fracture resistance.

scholarly journals Propolis: A Smart Supplement for an Intracanal Medicament

2017 ◽  
Vol 10 (4) ◽  
pp. 324-329
Author(s):  
Alok Avinash ◽  
Harsha Munot ◽  
Rashmi Baranwal ◽  
Vijay Duggi ◽  
Alok Dubey ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Propylene Glycol ◽  
Root Canal ◽  
Permanent Teeth ◽  
Dentinal Tubules ◽  
Group Iii ◽  
Ph Values ◽  
Group I ◽  
The Mean ◽  
Group Ii

ABSTRACT Introduction One of the most important factors for successful endodontic therapy is root canal cleaning. The difficulty involved in eliminating microorganisms, as well as their residual presence, warrants the use of root canal dressings after biomechanical preparation. Aim The aim of the study was to compare the diffusion ability between nonalcoholic calcium hydroxide—propolis paste, calcium hydroxide—saline paste, and calcium hydroxide—propylene glycol paste. Materials and methods For this proposed study, single-rooted extracted permanent teeth were randomly divided into three groups to fill the canals: group I: Calcium hydroxide—propylene glycol paste, group II: Calcium hydroxide—saline paste, and group III: Calcium hydroxide—propolis paste. After complete filing of the root canal, the pH values of the solutions in the flasks are measured at an interval of 3, 24, 72, 168 hours. Results After 168-hour interval, it was noticed that the mean pH obtained by calcium hydroxide—propolis paste was 10.54 (± 0.38), which was greater than calcium hydroxide—propylene glycol paste 9.70 (± 0.45) and calcium hydroxide—saline paste 9.16 (± 0.30) consecutively. Conclusion The nonalcoholic calcium hydroxide—propolis paste used during the study was able to diffuse through the dentinal tubules. Thus, it can be used as a vehicle for calcium hydroxide. How to cite this article Baranwal R, Duggi V, Avinash A, Dubey A, Pagaria S, Munot H. Propolis: A Smart Supplement for an Intracanal Medicament. Int J Clin Pediatr Dent 2017;10(4):324-329.

scholarly journals Scanning Electron Microscopic Evaluation of Marginal Adaptation of Three Endodontic Sealers: An Ex-Vivo Study

2021 ◽  
pp. 49-57
Author(s):  
Neeta Padmawar ◽  
Viddyasagar Moapagr ◽  
Vinay Vadvadgi ◽  
Jayshree Vishwas ◽  
Sourabh Joshi ◽  
...  
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
Marginal Adaptation ◽  
Scanning Microscope ◽  
Electron Microscopic ◽  
Microscopic Evaluation ◽  
Ah Plus ◽  
Root Dentin ◽  
Electron Scanning Microscope ◽  
Endodontic Sealers

Introduction: To avoid the entry of micro-organism or their products, sealer should completely adapt to the root canal wall so that no gaps will be present. AH plus is the most routinely used sealer. EndoRez is methacrylate resin-based self as well as light cured sealer. Endosequence BC RCS is the recently introduced bioceramic based sealer. Aim: To evaluate the marginal adaptation of Endosequence BS RCS, EndoRez and AH plus as a root canal sealer to root dentin under electron scanning microscope. Methods: Total 75 freshly extracted permanent maxillary central incisors with single canal were included in this study. All the teeth were decoronated till twelve millimeter and access cavity was prepared. All the teeth were prepared with rotary protaper till# F3 with intermediate copious irrigation with 3% sodium hypochlorite and 17%EDTA.Teeth were randomly divided into three groups according to sealers and were obturated- Endosequence BC RCS, Ah Plus and EndoRez. Access cavity was restored with glass ionomer type II in all the groups. After seven days, teeth were vertically sectioned and evaluated under electron scanning microscope for marginal adaptation. Results: Marginal gaps were present in all groups. Maximum number of gaps were present in Ah plus group. Endosequence BC RCS group showed least number of gaps. Discussion: Marginal adaptation of sealer depends upon the properties like flow, viscosity, presence or absence of smear layer etc. Better adaptation of Endosequence BC RCS to root dentin is due to formation of mineral infiltration zone which results in formation of calcific tags in dentine. Conclusion: Within the limitation of present study, newly introduced Endosequence BC RCS showed better marginal adaptability to root dentine. Further studies are required to evaluate and corelate these findings with other properties of these sealers.

scholarly journals Histopathological evaluation of root canal filling materials for primary teeth

2010 ◽  
Vol 21 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Léa Assed Bezerra da Silva ◽  
Mário Roberto Leonardo ◽  
Daniela S. B. de Oliveira ◽  
Raquel Assed Bezerra da Silva ◽  
Alexandra Mussolino de Queiroz ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Root Canal ◽  
Primary Teeth ◽  
Inflammatory Cells ◽  
Tissue Response ◽  
Root Canal Filling ◽  
Sterile Saline ◽  
Group Iii ◽  
Group I ◽  
Group Ii

This study aimed to assess the response of apical and periapical tissues of dogs' teeth after root canal filling with different materials. Forty roots from dogs' premolars were prepared biomechanically and assigned to 4 groups filled with: Group I: commercial calcium hydroxide and polyethylene glycol-based paste (Calen®) thickened with zinc oxide; Group II: paste composed of iodoform, Rifocort® and camphorated paramonochlorophenol; Group III: zinc oxide-eugenol cement; Group IV: sterile saline. After 30 days, the samples were subjected to histological processing. The histopathological findings revealed that in Groups I and IV the apical and periapical regions exhibited normal appearance, with large number of fibers and cells and no resorption of mineralized tissues. In Group II, mild inflammatory infiltrate and mild edema were observed, with discrete fibrogenesis and bone resorption. Group III showed altered periapical region and thickened periodontal ligament with presence of inflammatory cells and edema. It may be concluded that the Calen paste thickened with zinc oxide yielded the best tissue response, being the most indicated material for root canal filling of primary teeth with pulp vitality.

scholarly journals Comparative evaluation of Fracture Resistance of Endodontically Treated Teeth Obturated with Pozzolan-based MTA Sealer and Epoxy Resin-based Sealer: An in vitro Study

2017 ◽  
Vol 8 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Sree Theja Upadhyay ◽  
Tina Puthen Purayil
Keyword(s):  
Epoxy Resin ◽  
Root Canal ◽  
Fracture Resistance ◽  
Comparative Evaluation ◽  
In Vitro Study ◽  
Endodontically Treated Teeth ◽  
Group Iii ◽  
Group I ◽  
Ah Plus

ABSTRACT Aim To evaluate and compare the effect of epoxy resin-based sealer and a pozzolan-based mineral trioxide aggregate (MTA) sealer on the fracture resistance of endodontically treated teeth. Materials and methods Thirty single-rooted mandibular premolars were decoronated to a standardized root length of 15 mm. ProTaper rotary files up to a master apical file size of F3 were used for cleaning and shaping the root canals followed by 2.5% sodium hypochlorite irrigation. The teeth were randomly divided into three groups (n = 10 each), and the obturation was completed using gutta-percha with Endoseal MTA (group I) and AH Plus (group II) as root canal sealers. Group III served as control (instrumented and unfilled). Each specimen was then subjected to fracture testing by using a universal testing machine at a crosshead speed of 1.0 mm/minute until fracture. The force required to fracture each specimen was recorded and the data were subjected to statistical analysis using one-way analysis of variance (ANOVA), followed by pairwise comparison using post hoc Games-Howell test (p < 0.05). Results The fracture resistance of groups I and II were significantly higher than those of group III. No significant difference in the fracture resistance was observed between group I (Endoseal MTA) and group II (AH Plus) groups. Conclusion It can be concluded that the new root canal sealer, Endoseal MTA, is able to reinforce the tooth against fracture as good as AH Plus. Clinical significance Endoseal MTA is a sealer for the reinforcement of endodontically treated teeth. How to cite this article Upadhyay ST, Purayil TP, Ginjupalli K. Comparative evaluation of Fracture Resistance of EndodonticallyTreated Teeth Obturated with Pozzolan-based MTA Sealer and Epoxy Resin-based Sealer: An in vitro Study. World J Dent 2017;8(1):37-40.

Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

1970 ◽  
Vol 28 ◽  
pp. 208-209
Author(s):  
K.K. SEKHRI ◽  
C.S. ALEXANDER ◽  
H.T. NAGASAWA
Keyword(s):  
Osmium Tetroxide ◽  
Male Mice ◽  
Alcohol Group ◽  
Group Iii ◽  
Group I ◽  
C57bl Mice ◽  
Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

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