Envelhecimento acelerado em sementes de sorgo sacarino Sorghum bicolor (L.) Moench

O sorgo sacarino é cultivado a partir de sementes, necessitando técnicas adequadas de produção, colheita e secagem das mesmas para garantir sua disponibilidade e qualidade. Objetivou-se avaliar a qualidade inicial estudando uma metodologia mais adequada do teste de envelhecimento acelerado para a avaliação do potencial fisiológico de sementes de sorgo sacarino. A pesquisa foi conduzida no Laboratório Didático de Análise de Sementes do Departamento de Fitotecnia, Universidade Federal de Pelotas, em Pelotas, Rio Grande do Sul. Utilizou-se delineamento experimental inteiramente casualizado com 4 repetições. Foram utilizados quatro lotes de sementes de sorgo sacarino. Inicialmente, foi avaliada a qualidade inicial das sementes através dos seguintes testes: teor de água, germinação, primeira contagem de germinação e emergência a campo. Após, os lotes foram submetidos ao teste de envelhecimento tradicional e com uso se solução salina saturada (NaCl), nos períodos de 12, 24, 48, 72, 96 e 120h à 41 ºC. A exposição das sementes por 24h a 41 ºC no envelhecimento acelerado com uso de solução saturada de NaCl e por 48h a 41 ºC no envelhecimento acelerado tradicional são adequados para diferenciar lotes de sementes de sorgo sacarino.Accelerated aging in sorghum sacarino seeds Sorghum bicolor (L.) MoenchAbstract: Saccharin sorghum is cultivated from seeds, requiring adequate production, harvesting and drying techniques to ensure the availability of quality seed and, therefore. The objective was to evaluate the initial quality by studying a more adequate methodology of the accelerated aging test for the evaluation of the physiological potential of sorghum seeds. The research was conducted in the Laboratory of Seed Analysis of the Department of Plant Science, Federal University of Pelotas, in Pelotas, Rio Grande do Sul. A completely randomized experimental design with 4 replicates was used. Four lots of sweet sorghum seeds were used. First we assessed the quality of the seeds by the following tests: water content, germination, first count and emergency field. Following evaluation of initial quality, lots were submitted to the traditional aging test and use saturated saline, in periods of 12, 24, 48, 72, 96 and 120 hours at 41 °C. Exposure of seeds for 24 hours at 41 °C in accelerated aging with saturated NaCl and use for 48h at 41 °C in traditional accelerated aging are suitable to differentiate batches of sweet sorghum seeds.

Download Full-text

Genetic diversity for physiological quality of seeds from corn (Zea mays L.) intervarietal crossbreeds

Journal of Seed Science ◽

10.1590/s2317-15372013000400006 ◽

2013 ◽

Vol 35 (4) ◽

pp. 449-456 ◽

Cited By ~ 4

Author(s):

Daniele Nerling ◽

Cileide Maria Medeiros Coelho ◽

Rubens Onofre Nodari

Keyword(s):

Genetic Diversity ◽

Zea Mays L ◽

Accelerated Aging ◽

Genetic Contribution ◽

Percentage Germination ◽

Canonical Variables ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

The physiological quality is based on the genotype and may be accompanied since the first stages of selection through the study of genetic diversity. The purpose of this study was to evaluate the parental genetic contribution in the seed physiological quality in corn intervarietal crossbreeds. The intervarietal crossbreeds were performed using landraces genotypes, open pollinated varieties and commercial hybrids. The seeds obtained from the crossbreed, their equivalents and parentals were evaluated regarding their water percentage, germination, germination speed rate, accelerated aging, field emergence and electrical conductivity. The seeds obtained in the crossbreed are viable and vivid with heterosis when compared with the parentals. The accelerated aging test was more effective for selecting the strongest genotypes. The canonical variables were useful to group the genotypes that presented similar physiological characteristics. The genotypes MPA 01 x Pixurum 05, MPA 01 x AS1565, MPA 01 x SJC5886, SJC5886 x Pixurum 05 and Fundacep 35 x SCS 154 Fortuna were the best crossbreeds regarding the production of better physiological quality seeds.

Download Full-text

Electrical conductivity and accelerated aging in amaranth (Amaranthus crueentus L.) seeds

Journal of Seed Science ◽

10.1590/2317-1545v40n1183116 ◽

2018 ◽

Vol 40 (1) ◽

pp. 44-51

Author(s):

Thaís D’Avila Rosa ◽

Ariele Paula Nadal ◽

Henrique Roberto Maldaner ◽

Vanessa Nogueira Soares ◽

Gizele Ingrid Gadotti ◽

...

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Water Volume ◽

Amaranthus Cruentus ◽

Accelerated Aging Test ◽

Aging Test ◽

Immersion Period ◽

Amaranth Seed

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.

Download Full-text

Accelerated Aging Test to Determine the Vigor of Mungbean Seeds

Journal of Agricultural Science ◽

10.5539/jas.v11n7p42 ◽

2019 ◽

Vol 11 (7) ◽

pp. 42

Author(s):

Luciana Maria da Silva ◽

Anderson Dias Vaz de Souza ◽

Rogério Lámim Silva Junior ◽

Natália Arruda ◽

Nei Peixoto ◽

...

Keyword(s):

Seed Quality ◽

Germination Rate ◽

Accelerated Aging ◽

Dry Weight ◽

Precise Determination ◽

Germination Test ◽

Accelerated Aging Test ◽

Seedling Length ◽

Aging Test ◽

Randomized Experimental Design

The use of good quality seeds is fundamental for proper establishment of a crop. In this way, for more precise determination of seed quality, vigor tests are performed in addition to the germination test. These tests enable the selection of the best lots for commercialization and planting. This study evaluates the effects of temperature and exposure times of the accelerated aging test for vigor classification of mungbean seed lots. Seeds of the mungbean cultivar Esmeralda were used, being obtained from four cultivated plots at the State University of Goiás (UEG), Ipameri Câmpus, in the 2013, 2015, 2016, and 2017 harvests. The lots were initially characterized using germination and vigor tests (first germination count, germination rate index, seedling length, and seedling fresh and dry weight). The accelerated aging test was conducted with a completely randomized experimental design, in a 2 × 4 factorial arrangement consisting of two temperatures (40 and 42 °C) and four times (24, 48, 72, and 96 hours), with four replicates of 50 seeds per lot. The lots showed significant differences in the germination test, first germination count, germination rate index, and seedling length. The accelerated aging test was efficient in classifying lots. Lot 3 obtained the best results, while lot 1 obtained the lowest ones. The combination of 42 °C temperature and 72 h of seed exposure to the accelerated aging test is the best to classify mungbean seed lots.

Download Full-text

Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6sup2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

Download Full-text

QUALIDADE FISIOLÓGICA DE SEMENTES DE SOJA SUBMETIDAS À DESSECAÇÃO EM PRÉ-COLHEITA

Colloquium Agrariae ◽

10.5747/ca.2018.v14.n4.a254 ◽

2018 ◽

Vol 14 (4) ◽

pp. 112-120

Author(s):

Renan Canzi Comin ◽

Geri Eduardo Meneghello ◽

Jader Job Franco ◽

Alexandre Moscarelli Levien ◽

Kassiana Kehl ◽

...

Keyword(s):

Seed Quality ◽

Accelerated Aging ◽

Glufosinate Ammonium ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Important Idea ◽

The City ◽

Technical Recommendations

The use of high quality soybean seeds (Glycine maxL.) is one of the most important aspects to obtain satisfactory productivity indexes. The desiccation practice is an important idea of the producers to anticipate theharvest and to minimize the rapiddeterioration of seed quality in the field. In this context, the objective of this work was to evaluate the effect of the use of desiccants applied in R7stageon the physiological quality of soybean seeds.The experiment was conducted in experimental area, located in the city of Passo Fundo, Rio Grande do Sul. Four soybean cultivars (FPS Jupiter RR, FPS Paranapanema RR, BRS Tordilha RR and FPS Urano RR) were planted under experimental design, managed according to technical recommendations for the crop.Upon reaching the R7 stage, different desiccation treatments (without desiccation, paraquat, diquat and glufosinate ammonium) were applied to the subplot.The seeds were harvested with a plot harvester and the weight of one thousand seeds, germination test and accelerated aging test were evaluated, being the last two evaluated at 30 and 180 days after harvest. The pre-harvest desiccation at the R7 stage of soybean cultivation did not negatively affect the physiological quality of the seeds and, generally way, for the cultivars FPS Jupiter RR and FPS Urano RR, it was possible to obtain more vigorous seeds at 30 DAC. Under the conditions under which the experiment was conducted, the diquat, paraquat and glufosinate ammonium herbicides can be used for pre-harvest desiccation in the seed production fields.

Download Full-text

Seed quality parameters of three interspecific hybrids of elephant grass x pearl millet

Revista Brasileira de Sementes ◽

10.1590/s0101-31222012000300004 ◽

2012 ◽

Vol 34 (3) ◽

pp. 382-387

Author(s):

Marina Pozitano ◽

Roberto Usberti

Keyword(s):

Pearl Millet ◽

Recurrent Selection ◽

Seed Quality ◽

Accelerated Aging ◽

Pennisetum Purpureum ◽

Elephant Grass ◽

Accelerated Aging Test ◽

Aging Test ◽

Drying Curves

An interspecific hybrid resulting from the crossing of elephant grass (Pennisetum purpureum Schumach) x pearl millet (Pennisetum glaucum (L.) R. Brown) has been developed. This hybrid, however, revealed low phenotypic uniformity and low production of pure seeds. Through recurrent selection, two improved populations were obtained (genotypes Corte and Pastoreio). The aim of this study was assessing seed quality of the three hybrids (genotypes Corte, Pastoreio and Paraiso) by tests of: seed purity; seed germination; accelerated aging test, at 42 ºC; 1,000 seeds weight; drying curves; and sorption and desorption isotherms. Recurrent selection altered the seed size and increased initial quality of population for genotype Pastoreio. Drying curves for the three hybrids have shown similar behavior and reached moisture contents of 2.1%, 1.9%, and 1.8%, respectively, after 63 days. The accelerated aging test showed that hybrid Pastoreio was the most vigorous.

Download Full-text

Slurry Composition and Physiological Quality of Treated Soybean Seeds Over Storage

Journal of Agricultural Science ◽

10.5539/jas.v11n1p376 ◽

2018 ◽

Vol 11 (1) ◽

pp. 376

Author(s):

Andréia K. Suzukawa ◽

Giovanna E. G. Mariucci ◽

Lucas C. Pereira ◽

Alessandro L. Braccini ◽

Raissa M. Ponce ◽

...

Keyword(s):

Repeated Measures ◽

Accelerated Aging ◽

Storage Period ◽

Soybean Seeds ◽

Time Model ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Slurry Composition

Industrial seed treatment assures uniform coverage of seeds with insecticides, fungicides, micronutrients and bioestimulant at precise dose, however often resulting in higher slurry volume. Furthermore, seeds are coated long periods of time prior sowing and may influence germination and vigor. Thus, the aim of this work was to evaluate the influence of seven industrial seed treatments and their respective slurry volumes on the physiological potential of soybean seeds at 0, 15, 30, 45, 60 and 90 days of storage. In each storage period, the variables germination, accelerated aging test and emergency speed index were evaluated and studied in the repeated measures in time model. The products used were: fungicide (thiabendazole, fludioxonil and mefenoxam), insecticide (thiametoxam), insecticide/nematicide (abamectin), micronutrients (cobalt and molybdenum), bioregulator, drying powder and polymer. High volumes of seed-coating mixtures reduce the physiological quality of soybean seeds over storage. However, the slurry composition also influenced on the maintenance of the seed germination and vigor throughout the storage.

Download Full-text

Physiological quality of popcorn seeds assessed by the accelerated aging test

Journal of Seed Science ◽

10.1590/2317-1545v40n4191101 ◽

2018 ◽

Vol 40 (4) ◽

pp. 428-434 ◽

Cited By ~ 2

Author(s):

Cristiane da Silva Rocha ◽

Hugo César Rodrigues Moreira Catão ◽

Franciele Caixeta ◽

Heloisa Karoline Kavan ◽

Talis Melo Claudino ◽

...

Keyword(s):

Electrical Conductivity ◽

Moisture Content ◽

Accelerated Aging ◽

Seed Moisture Content ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Consistent Information

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.

Download Full-text

SUITABILITY OF ELECTRICAL CONDUCTIVITY TESTING AND ACCELERATED AGING FOR EVALUATING THE VIGOR OFCITHAREXYLUM MYRIANTHUM CHAM. DIASPORES

FLORESTA ◽

10.5380/rf.v49i4.57765 ◽

2019 ◽

Vol 49 (4) ◽

pp. 701

Author(s):

Ana Paula Bazzanella ◽

Gabriele Larissa Mathias ◽

Henrique Roberto Gattermann Mittelstaedt ◽

Maiara Iadwizak Ribeiro ◽

Andréa Maria Teixeira Forte ◽

...

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Germination Test ◽

High Demand ◽

Tukey Test ◽

Aging Period ◽

Accelerated Aging Test ◽

Aging Test ◽

Randomized Experimental Design ◽

Native Tree Species

The Atlantic Forest is one of the most threatened Brazilian biomes by anthropic action. Thus, the interest inrecovering degraded areas through the use of native tree species has increased, causing a high demand in the use of vigorous seeds. This research aimed at evaluating the suitabilityof the electrical conductivity and accelerated aging tests to evaluate the vigor of C. myrianthum diaspores. For the adequacy of the electrical conductivity test, a completely randomized experimental design (CRD) was used in a 2 x 3 x9 (number of diaspores x volume of water x imbibition time)at a constant temperature of 25 °C. The procedure for the accelerated aging test was conducted at 40 ºC for 0 (no aging), 24, 48 and 72 hours. ACRDwas also used for the accelerated aging test, totaling 4 treatments. The results were submitted to analysis of variance (ANOVA) and the means were compared by the Tukey test at 5% probability. It is concluded that the electrical conductivity test for C. myrianthum diaspores can be conducted with 25 diaspores soaked with 50 mL of deionized water for 96 hours, thus replacing the germination test with the advantage of being carried out in a shorter period of time. Moreover,the 24 and 48 hour accelerated aging period is adequate to discriminate the vigor of C. myrianthum diaspores, with GSI and MGT being the most sensitive variables.

Download Full-text

Accelerated aging test for forage pea seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n5p1819 ◽

2019 ◽

Vol 40 (5) ◽

pp. 1819

Author(s):

Carla Gomes Machado ◽

Givanildo Zildo Silva ◽

Silvia Sanielle Costa Oliveira ◽

Cibele Chalita Martins

Keyword(s):

Electrical Conductivity ◽

Potassium Chloride ◽

Seedling Emergence ◽

Accelerated Aging ◽

Cold Climate ◽

Accelerated Aging Test ◽

Aging Test ◽

Pea Seeds ◽

Pea Seed

Forage pea is an important annual, cold climate crop with high levels of essential amino acids in its seeds, which are lacking in the composition of cereal grains. Therefore, livestock farmers use its seeds as a nutritious source of forage. To plant fields of this forage, it is necessary to know the quality of the seeds, determined through germination and vigor tests. In the literature, studies on vigor tests for forage pea seeds are scarce. This study aimed to verify the efficiency of vigor tests, with emphasis on accelerated aging to evaluate the physiological quality of forage pea seeds in order to select lots for seedling emergence in the field. Using accelerated aging tests, 10 lots of forage pea were evaluated for moisture content, thousand-seed weight, emergence percentage, emergence first count, speed index of seedlings in the field, germination, germination first count, and electrical conductivity. The following variations of methodology were used: temperatures of 41 and 42 °C, exposure times of 48 and 72 h, and different relative humidity values in the aging atmosphere, using distilled water (100 % RH), saturated solution of potassium chloride (87 % RH), and sodium chloride (76 % RH). The tests of germination, emergence first count of seedlings in the field, emergence speed index, and electrical conductivity are promising to evaluate the vigor of forage pea seed lots. The accelerated aging test conducted in a solution saturated with potassium chloride at 42 °C for 48 h is adequate to estimate the vigor of forage pea seed lots, providing information similar to that of the emergence of seedlings in the field.

Download Full-text