THE IMPACT OF NUTRITION ON THE COURSE OF LICHEN PLANUS

Relevance: red lichen planus refers to a long, protracted disease with a chronic course. There are a lot of root causes, it is quite diﬃcult to diﬀerentiate them. Therefore, pharmacotherapy of this pathology is reduced to the appointment of local drugs that have only symptomatic eﬀects that help accelerate the healing of the oral mucosa.Purpose – to study reparative regeneration based on the analysis of clinical and cytological examination on the background of the appointment of various methods of pharmacotherapy.Materials and methods: all patients were divided into two groups using simple randomization: in patients of the ﬁrst group, betamethasone B was used to treat erosive-ulcerative lesions, which were delivered to the lesion using Tisol, which has conductive transcutaneous activity, using the sandwich technique. The second group of patients used the drug delivery system in the form of a ﬁlm, which ﬁxed betamethasone B in the lesion area.Results: were evaluated before treatment, on day 7 and 14. The area and intensity of healing of the oral mucosa were determined, a qualitative and quantitative analysis of cytograms was carried out, which characterizes the dynamics of reparative regeneration of erosions and ulcers.Conclusion: based on the obtained clinical and laboratory data, it can be concluded that it is advisable to include betamethasone applications in the pharmacotherapy of patients with erosive and ulcerative forms of lichen planus. However, preference should be given to the method of layer-by-layer application of betamethasone B in combination with Tezol.

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Inheritable testicular metabolic memory of high-fat diet causes transgenerational sperm defects in mice

Scientific Reports ◽

10.1038/s41598-021-88981-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Luís Crisóstomo ◽

Ivana Jarak ◽

Luís P. Rato ◽

João F. Raposo ◽

Rachel L. Batterham ◽

...

Keyword(s):

Sperm Quality ◽

Feeding Habits ◽

Reproductive Function ◽

Principal Component ◽

Testicular Tissue ◽

Sperm Parameters ◽

Metabolic Memory ◽

High Fat ◽

And Function ◽

The Impact

AbstractThe consumption of energy-dense diets has contributed to an increase in the prevalence of obesity and its comorbidities worldwide. The adoption of unhealthy feeding habits often occurs at early age, prompting the early onset of metabolic disease with unknown consequences for reproductive function later in life. Recently, evidence has emerged regarding the intergenerational and transgenerational effects of high-fat diets (HFD) on sperm parameters and testicular metabolism. Hereby, we study the impact of high-fat feeding male mice (F0) on the testicular metabolome and function of their sons (F1) and grandsons (F2). Testicular content of metabolites related to insulin resistance, cell membrane remodeling, nutritional support and antioxidative stress (leucine, acetate, glycine, glutamine, inosine) were altered in sons and grandsons of mice fed with HFD, comparing to descendants of chow-fed mice. Sperm counts were lower in the grandsons of mice fed with HFD, even if transient. Sperm quality was correlated to testicular metabolite content in all generations. Principal Component Analysis of sperm parameters and testicular metabolites revealed an HFD-related phenotype, especially in the diet-challenged generation and their grandsons. Ancestral HFD, even if transient, causes transgenerational “inherited metabolic memory” in the testicular tissue, characterized by changes in testicular metabolome and function.

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Early-Life Development of the Bifidobacterial Community in the Infant Gut

International Journal of Molecular Sciences ◽

10.3390/ijms22073382 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3382

Author(s):

Silvia Saturio ◽

Alicja M. Nogacka ◽

Marta Suárez ◽

Nuria Fernández ◽

Laura Mantecón ◽

...

Keyword(s):

Gut Microbiota ◽

Gestational Age ◽

Early Life ◽

Feeding Habits ◽

Its Region ◽

23S Rrna ◽

Delivery Mode ◽

Short And Long Term ◽

Quantitative Changes ◽

The Impact

The establishment of the gut microbiota poses implications for short and long-term health. Bifidobacterium is an important taxon in early life, being one of the most abundant genera in the infant intestinal microbiota and carrying out key functions for maintaining host-homeostasis. Recent metagenomic studies have shown that different factors, such as gestational age, delivery mode, or feeding habits, affect the gut microbiota establishment at high phylogenetic levels. However, their impact on the specific bifidobacterial populations is not yet well understood. Here we studied the impact of these factors on the different Bifidobacterium species and subspecies at both the quantitative and qualitative levels. Fecal samples were taken from 85 neonates at 2, 10, 30, 90 days of life, and the relative proportions of the different bifidobacterial populations were assessed by 16S rRNA–23S rRNA internal transcribed spacer (ITS) region sequencing. Absolute levels of the main species were determined by q-PCR. Our results showed that the bifidobacterial population establishment is affected by gestational age, delivery mode, and infant feeding, as it is evidenced by qualitative and quantitative changes. These data underline the need for understanding the impact of perinatal factors on the gut microbiota also at low taxonomic levels, especially in the case of relevant microbial populations such as Bifidobacterium. The data obtained provide indications for the selection of the species best suited for the development of bifidobacteria-based products for different groups of neonates and will help to develop rational strategies for favoring a healthy early microbiota development when this process is challenged.

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Malignant development of lichen planus-affected oral mucosa

Journal of Oral Pathology and Medicine ◽

10.1111/j.1600-0714.1988.tb01528.x ◽

1988 ◽

Vol 17 (5) ◽

pp. 219-225 ◽

Cited By ~ 156

Author(s):

P. Holmstrup ◽

J. J. Thorn ◽

J. Rindum ◽

J. J. Pindborg

Keyword(s):

Lichen Planus ◽

Oral Mucosa

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The Lichenoid Tissue Reactions of the Oral Mucosa: Oral Lichen Planus and Other Lichenoid Lesions

Surgical Pathology Clinics ◽

10.1016/j.path.2011.07.001 ◽

2011 ◽

Vol 4 (4) ◽

pp. 1005-1026 ◽

Cited By ~ 7

Author(s):

Susan Müller

Keyword(s):

Lichen Planus ◽

Oral Mucosa ◽

Oral Lichen Planus ◽

Tissue Reactions ◽

Oral Lichen

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Criteria for assessing severity of oral lichen planus

MedAlliance ◽

10.36422/23076348-2021-9-1-73-81 ◽

2021 ◽

Vol 9 (1) ◽

pp. 73-81

Keyword(s):

Lichen Planus ◽

Oral Mucosa ◽

Severity Score ◽

Oral Lichen Planus ◽

Unknown Etiology ◽

Altered Level ◽

Doppler Flowmetry ◽

Serum Free ◽

Control Subjects ◽

Oral Lichen

Introduction. Oral lichen planus (OLP) is an autoim- mune disease of unknown etiology, manifested in vari- ous clinical forms. A correlation between intensity of OLP local manifestations and severity of general disturbances predisposing to altered oral mucosa have been poorly investigated. The aim of our study was to identify criteria for assessing OLP severity score. Methods. OLP patients and control subjects (not OLP) matched by hepatobiliary disease and type IIa dyslipidemia were examined. Samples collected during the study were as- sessed by way clinical examination, gas chromatographic analysis of serum free fatty acids (FFA), oral fluid, serum and fecal microbial marker analysis by gas chromatogra- phy/mass spectrometry, laser Doppler flowmetry of the oral mucosa, immunohistochemistry staining with an- ti-CD68 monoclonal antibody specific to apolipoprotein B-100-bearing macrophages. Results. It was found that OLP patients significantly differed from control subjects in the three FFA levels (lauric acid, eicosopentaenoic/ lauric and eicosopentaenoic/linoleic ratios), displayed four pathologic factors matching the altered level of mi- crobial markers. In addition, OLP patients were found to differ in oral mucosa blood filling both inside and outside lesions paralleled with oxidative changes immediately in oral mucosa highlighted as elevated amount of apo- lipoprotein B-100-bearing CD68-positive macrophages. Conclusion. OLP severity score is an integrate parameter which reflects oxidative changes and alterations in oral mucosa microhemocirculation primarily in serum free fat- ty acid composition, altered oral and gut microbiota as well as intensity score of clinical picture.

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Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

Brazilian Dental Journal ◽

10.1590/0103-6440201302379 ◽

2014 ◽

Vol 25 (6) ◽

pp. 461-465

Author(s):

João Paulo De Carli ◽

Soluete Oliveira da Silva ◽

Maria Salete Sandini Linden ◽

Carmen Silvia Busin ◽

Luiz Renato Paranhos ◽

...

Keyword(s):

Hepatitis C ◽

Lichen Planus ◽

Oral Mucosa ◽

Oral Lichen Planus ◽

Clinical Signs ◽

Hcv Infection ◽

Control Group ◽

Proliferative Potential ◽

Group 2 ◽

Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

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Role of apoptosis in erosive and reticular oral lichen planus exhibiting variable epithelial thickness

Brazilian Dental Journal ◽

10.1590/s0103-64402008000300001 ◽

2008 ◽

Vol 19 (3) ◽

pp. 179-185 ◽

Cited By ~ 21

Author(s):

Juliana M. Caldeira Brant ◽

Anilton C. Vasconcelos ◽

Luciana V. Rodrigues

Keyword(s):

Lichen Planus ◽

Oral Mucosa ◽

Oral Lichen Planus ◽

Chronic Inflammatory Disease ◽

Mechanical Trauma ◽

Epithelial Thickness ◽

Healthy Mucosa ◽

Toxic Agents ◽

Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical types. Reticular and erosive forms are the most common. Although the cause of OLP remains speculative, many findings suggest auto-immune involvement, mediated by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma or toxic agents can affect the epithelial homeostasia. Increased apoptosis may cause a decrease in epithelial thickness reflecting in the activity of the lesion. The objective of this study was to evaluate the occurrence of apoptosis and epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each type (reticular and erosive) plus 10 of healthy mucosa were collected and processed. After morphometry, the apoptotic index and epitelial thickness were obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate the morphologic criteria used. Apoptosis in the erosive OLP was significantly more intense than in the reticular type and both forms of OLP presented more apoptosis than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms and thicker in OLP reticular form than in the erosive one. The clinical differences between reticular and erosive forms of OLP are related to variations in epithelial thickness and in intensity of apoptosis.

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Eco Friendly Lighting Options for Offshore Installations and Related Design Challenges

10.2118/207994-ms ◽

2021 ◽

Author(s):

Sathishkumar Pragasam

Keyword(s):

Migratory Birds ◽

Feeding Habits ◽

Reproductive Function ◽

Marine Species ◽

Offshore Structures ◽

Marine Animals ◽

Artificial Lighting ◽

Migratory Flight ◽

Offshore Installations ◽

The Impact

Abstract The impact of artificial lighting on birds and animals has been a subject of research for many decades. These studies have established that light pollution from offshore facilities result in disturbing effects on migratory birds and marine animals. It is understood from the various studies that light emanating from offshore structures attract birds at night and disorient them during their migratory flight. As they are distracted from their flight path, they are either found trapped by the light that they keep circling around the installations or make them to take shelter in the installations, ultimately making them miss their migratory journey. This results in reduced winter survival and reduced reproduction success. Many birds are also found to die eventually because of bird strike or by getting themselves incinerated in flares. Congregation of birds in offshore installations also lead to risks related to helicopter landing / take-off. It is also observed that artificial light attracts marine species like turtles and fishes, which result in adverse effects like alteration of feeding habits, impacted circadian rhythm and interference with their reproductive function. From the knowledge of aforementioned disruptive effects of artificial lighting, significant efforts have been made to develop lighting systems for offshore installations that are eco-friendly. Various means of mitigating the undesirable effects of artificial lighting in offshore installations are being tried in the last couple of decades.

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