scholarly journals A comparative study of volatile fatty acids in the blood and in the drainage from pancreatic fistula in pancreatic necrosis

2018 ◽  
Vol 11 (4) ◽  
pp. 232-236 ◽  
Author(s):  
Alexander Sergeevich Мetelev ◽  
Eduard S Akayzin ◽  
Alexander K Gagua
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Pancreatic Necrosis ◽  
Capillary Column ◽  
Isovaleric Acid ◽  
Gas Liquid Chromatography ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Healthy Donors

Relevance. The informative value of the content of volatile fatty acids (VFA) in the fistulous drainage from pancreas not been studied in the diagnosis of pancreonecrosis (PN). The informative value of the indices of the content of VFA in the blood has not been studied sufficiently in the diagnosis of PN. Aim of the study is to compare the content of VFA in the blood and in the fistulous drainage from pancreas in patients with pancreatic necrosis. Materials and methods. Samples of blood and fistulous drainage from pancreas isolated from patients with a confirmed diagnosis of PN were studied (n = 18). There was analysis of the concentrations of VFA: acetic, propionic, butyric and isovaleric acids by gas-liquid chromatography on an automated gas chromatograph "Crystallux-4000" with a capillary column "HP-FFAP" Agilent Technologies and a flame ionization detector. The anaerobic index and sum of VFA were calculated. Results. Higher values of the content of acetic, propionic and butyric acids, the sum of VFA and lower values of the content of isovaleric acid and anaerobic index were found in patients with PN in comparison with those of practically healthy donors. Higher values of the content of acetic, propionic and isovaleric acid, the sum of VFA and anaerobic index were noted in the fistulous drainage from pancreas in comparison with the same parameters in blood in patients with PN. The correlation of pairwise conjugated parameters of the content of propionic and isovaleric acids, anaerobic index and the sum of VFA in fistulous drainage from pancreas and blood are discovered in patients with PN. Conclusions. The concentration of acetic, propionic and isovaleric acids, the sum of VFA were higher in the fistulous drainage from pancreas than in the blood in patients with PN. The analysis of the parameters of the VFA can be used as additional criteria for the early diagnosis of PN.

INDICATORS OF VOLATILE FATTY ACIDS IN DIFFERENTIAL DIAGNOSTICS OF INFECTED AND STERILE PANCREONECROSIS

2019 ◽  
Vol 64 (11) ◽  
pp. 644-648
Author(s):  
E. S. Akayzin ◽  
A. S. Metelev ◽  
A. E. Akayzina
Keyword(s):  
Fatty Acids ◽  
Differential Diagnosis ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Pancreatic Necrosis ◽  
Capillary Column ◽  
Differential Diagnostics ◽  
Gas Chromatograph ◽  
Flame Ionization

The aim of the study was to assess the information content of volatile fatty acid parameters for the differential diagnosis of infected and sterile pancreatic necrosis. The work is based on the results of examination and treatment of 34 patients with pancreatic necrosis. The analysis of concentrations of volatile fatty acids: acetic, propionic, butyric and isovaleric was carried out on an automated gas chromatograph «Crystallux-4000» with a capillary column «HP-FFAP» and flame ionization detector. The indicators of acetic, propionic, butyric, isovaleric acid and the sum of volatile fatty acids are statistically significantly higher in patients with infected pancreatic necrosis compared with the indicators of volatile fatty acids in patients with sterile pancreatic necrosis. Volatile fatty acid values can be used for the differential diagnosis of infected and sterile pancreatic necrosis

Indicators of volatile fatty acids for differential diagnostics of nfected of pancreatic necrosis complicated by retroperitoneal phlegmon

2021 ◽  
Vol 66 (5) ◽  
pp. 310-314
Author(s):  
E. S. Akayzin ◽  
A. E. Akayzina
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Differential Diagnosis ◽  
Volatile Fatty Acids ◽  
Pancreatic Necrosis ◽  
Capillary Column ◽  
Infected Pancreatic Necrosis ◽  
Differential Diagnostics ◽  
Gas Chromatograph ◽  
Flame Ionization

The aim of the study was to assess the informative value of volatile fatty acid parameters for the differential diagnosis of infected pancreatic necrosis (IPN), complicated by retroperitoneal cellulitis. The work was based on the results of examination and treatment of 44 patients with infected pancreatic necrosis. The analysis of concentrations of volatile fatty acids: acetic, propionic, butyric and isovalerianic was carried out on a Kristallux-4000 automated gas chromatograph with an HP-FFAP capillary column and a flame ionization detector. The indicators of acetic acid and the amount of volatile fatty acids were statistically significantly higher in patients with infected pancreatic necrosis with total retroperitoneal phlegmons in comparison with the indicators of volatile fatty acids in patients with infected pancreatic necrosis with paracolic phlegmons and in comparison with indicators of volatile fatty acids in patients with infected pancreatic necrosis phlegmons. The indicators of acetic acid and the amount of VFA can be used as additional criteria for the differential diagnosis of IPN complicated by phlegmons of the retroperitoneal tissue.

scholarly journals Determination of N-Nitrosodimethylamine in the Smoke of High-Nitrate Tobacco Cigarettes

1973 ◽  
Vol 7 (2) ◽  
Author(s):  
G.P. Morie ◽  
C.H. Sloan
Keyword(s):  
Capillary Column ◽  
Glass Capillary Column ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Glass Capillary ◽  
Flame Ionization ◽  
Basic Solutions ◽  
Poly Ethylene ◽  
Gas Chromatographic Method

AbstractA gas chromatographic method was developed for the determination of N-nitrosodimethylamine (NDMA) in cigarette smoke. The NDMA in the smoke of 200 cigarettes was trapped in a solution of sodium hydroxide and separated from most of the smoke components by distillation from acidic and basic solutions. The aqueous solution was extracted for 8 hr. into ethyl ether in a Dean Stark apparatus. To concentrate the solution of NDMA, the ether was distilled until only 5 ml of the solution remained. An aliquot of this solution was analysed by means of a gas chromatograph equipped with a 200-ft. glass capillary column coated with Carbowax 20-M poly(ethylene glycoI). An alkali metal flame ionization detector with a selectivity of 10*/1 for nitrogen compounds to normal hydrocarbons was used. Small amounts (2 ng) of nitrosamines in the presence of large amounts of other compounds were easily detected. N-nitrosodimethylamine-C

Determination of volatile alcohols and acetone in serum by non-polar capillary gas chromatography after direct sample injection.

1984 ◽  
Vol 30 (10) ◽  
pp. 1672-1674 ◽  
Author(s):  
N B Smith
Keyword(s):  
Gas Chromatography ◽  
Capillary Gas Chromatography ◽  
Capillary Column ◽  
Internal Standard ◽  
Fused Silica ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Split Ratio

Abstract In this method for detection and quantification of volatile alcohols by capillary gas chromatography, the serum sample is deproteinized, then directly injected into the gas chromatograph with 1-propanol as the internal standard. The capillary column is a 30-m bonded methylsilicone-coated, fused-silica column. With helium as the carrier gas, the injector inlet is set at a split ratio of 1/30 and the average linear velocity in the column is 25 cm/s. Injector and flame-ionization detector temperatures are 280 degrees C, oven temperature 35 degrees C. Chromatography time is less than 3 min.

Gas-Liquid Chromatography of Bromoxynil Octanoate

1977 ◽  
Vol 60 (4) ◽  
pp. 865-867
Author(s):  
Laurence J Helfant
Keyword(s):  
Direct Method ◽  
Standard Procedure ◽  
Internal Standard ◽  
Gas Liquid Chromatography ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Weight Percentage ◽  
Flame Ionization ◽  
Pesticide Formulations ◽  
By Products

Abstract A simple, direct method of analysis is proposed that will determine the weight percentage of bromoxynil octanoate in pesticide formulations. Using a gas chromatograph equipped with a flame ionization detector, the ester is separated from its isomers and other reaction by-products, and then quantitated by using an internal standard procedure. This method gives a specific percentage for bromoxynil octanoate, compared with halogen content derived from the Parr bomb procedure that is currently used.

scholarly journals Fatty acid profile of Slovenian farmed rainbow trout

Meso ◽  
2021 ◽  
Vol 23 (3) ◽  
pp. 202-209
Author(s):  
Tomaž Polak ◽  
Mateja Lušnic Polak ◽  
Andrea Rukavina ◽  
Lea Demšar
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Rainbow Trout ◽  
Fatty Acid Profile ◽  
Fatty Acids Composition ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Fish Farms ◽  
Flame Ionization ◽  
Long Chain

The present study was carried out to determine the fatty acid profile of rainbow trout (Oncorynchus mykiss) from three Slovenian fish farms; Zalog, Želimlje and Povodje. Fatty acids composition was determined on a gas chromatograph with a flame ionization detector (GC-FID). The results showed that farming conditions have a significant influence on the fatty acid composition of rainbow trout. The predominant saturated fatty acid (SFA) was palmitic acid, oleic acid was the main monounsaturated fatty acid (MUFA), while the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were the main long chain n-3 polyunsaturated fatty acids (n-3 PUFA). The percentage of DHA exceeded that of EPA in all rainbow trout samples studied. The n-6/n-3 ratio ranged from 0.89 to 1.54 and the PUFA/SFA ratio was between 1.81 and 2.36. In dorsal and ventral fillet parts, the content of most fatty acids was similar, exceptions were observed for some PUFAs; arachidonic acid, EPA and DHA

Ozonolysis of unsaturated fatty acids. II. Esterification of the total products from the oxidative decomposition of ozonides with 2,2-dimethoxypropane

1967 ◽  
Vol 45 (13) ◽  
pp. 1405-1410 ◽  
Author(s):  
John D. Castell ◽  
R. G. Ackman
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Unsaturated Fatty Acids ◽  
Performic Acid ◽  
Acid Oxidation ◽  
Gas Liquid Chromatography ◽  
Positional Isomers ◽  
Flame Ionization ◽  
Hydrogen Flame

The total acidic products from the performic acid oxidation of the ozonide of methyl oleate formed in methanol may be esterified directly in a few hours with 2,2-dimethoxypropane. The ester concentrations are adequate for the determination of the positional isomers of monoethylenic fatty acids directly from the reaction mixture, using a hydrogen flame ionization gas–liquid chromatography detector. Dimethyl sulfoxide was not required to prevent the breakdown of 2,2-dimethoxypropane under the conditions employed.

INCIDENCES OF POLYCYCLIC AROMATIC HYDROCARBONS IN ROASTED PLANTAINS OBTAINED WITHIN OSOGBO METROPOLIS, NIGERIA

2021 ◽  
Vol 21 (2) ◽  
pp. 539-546
Author(s):  
ABIONA OLUSEYE OLADAPO ◽  
ADEGUNWA ABIODUN ODUNLAMI ◽  
AWOJIDE SHOLA HEZEKIAH ◽  
ANIFOWOSE ADEBANJO JACOB ◽  
TAYO ADENIYI SOLOMON
Keyword(s):  
Soxhlet Extraction ◽  
Carcinogenic Risk ◽  
Diagnostic Ratios ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Total Pahs ◽  
Flame Ionization ◽  
Regulatory Limits ◽  
Polycyclic Aromatic ◽  
Extracting Solvent

The present study was conducted to assess the carcinogenic and non-carcinogenic health risks of PAHs contamination in roasted plantain collected from three different locations within Osogbo metropolis, Nigeria. The PAHs were extracted using Soxhlet extraction technique with n-hexane and dichloromethane (3:1) as the extracting solvent. A column, packed with silica gel, was used for clean-up process and the levels of the targeted PAHs were determined by a gas chromatograph–flame ionization detector (GC-FID). The result revealed that the concentrations of total PAHs detected in the roasted samples at location A, B, C were 3.958 μg/kg, 3.654 μg/kg and 3.217 μg/kg. The HMW carcinogenic PAHs constitute about 32% of the total PAHs in the roasted plantain samples where benzo(a)pyrene, which is a marker for carcinogenicity, was found to be 9%. Comparing these results with the regulatory limits, all the analyzed roasted plantain samples were found below the maximum residual limit (MRL) of 5 μg/kg, but the calculated carcinogenic risk (CR) presented value greater than 1×10-4, signifying health risk associated with the consumption of the roasted plantain. The major source of the PAHs using diagnostic ratios (Fla/Pyr and Ph/Ant) showed that the charcoal used in roasting is a major contributing factor to the high levels of PAHs detected in the roasted plantain samples.

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