Indicators of volatile fatty acids for differential diagnostics of nfected of pancreatic necrosis complicated by retroperitoneal phlegmon

2021 ◽  
Vol 66 (5) ◽  
pp. 310-314
Author(s):  
E. S. Akayzin ◽  
A. E. Akayzina
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Differential Diagnosis ◽  
Volatile Fatty Acids ◽  
Pancreatic Necrosis ◽  
Capillary Column ◽  
Infected Pancreatic Necrosis ◽  
Differential Diagnostics ◽  
Gas Chromatograph ◽  
Flame Ionization

The aim of the study was to assess the informative value of volatile fatty acid parameters for the differential diagnosis of infected pancreatic necrosis (IPN), complicated by retroperitoneal cellulitis. The work was based on the results of examination and treatment of 44 patients with infected pancreatic necrosis. The analysis of concentrations of volatile fatty acids: acetic, propionic, butyric and isovalerianic was carried out on a Kristallux-4000 automated gas chromatograph with an HP-FFAP capillary column and a flame ionization detector. The indicators of acetic acid and the amount of volatile fatty acids were statistically significantly higher in patients with infected pancreatic necrosis with total retroperitoneal phlegmons in comparison with the indicators of volatile fatty acids in patients with infected pancreatic necrosis with paracolic phlegmons and in comparison with indicators of volatile fatty acids in patients with infected pancreatic necrosis phlegmons. The indicators of acetic acid and the amount of VFA can be used as additional criteria for the differential diagnosis of IPN complicated by phlegmons of the retroperitoneal tissue.

INDICATORS OF VOLATILE FATTY ACIDS IN DIFFERENTIAL DIAGNOSTICS OF INFECTED AND STERILE PANCREONECROSIS

2019 ◽  
Vol 64 (11) ◽  
pp. 644-648
Author(s):  
E. S. Akayzin ◽  
A. S. Metelev ◽  
A. E. Akayzina
Keyword(s):  
Fatty Acids ◽  
Differential Diagnosis ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Pancreatic Necrosis ◽  
Capillary Column ◽  
Differential Diagnostics ◽  
Gas Chromatograph ◽  
Flame Ionization

The aim of the study was to assess the information content of volatile fatty acid parameters for the differential diagnosis of infected and sterile pancreatic necrosis. The work is based on the results of examination and treatment of 34 patients with pancreatic necrosis. The analysis of concentrations of volatile fatty acids: acetic, propionic, butyric and isovaleric was carried out on an automated gas chromatograph «Crystallux-4000» with a capillary column «HP-FFAP» and flame ionization detector. The indicators of acetic, propionic, butyric, isovaleric acid and the sum of volatile fatty acids are statistically significantly higher in patients with infected pancreatic necrosis compared with the indicators of volatile fatty acids in patients with sterile pancreatic necrosis. Volatile fatty acid values can be used for the differential diagnosis of infected and sterile pancreatic necrosis

scholarly journals A comparative study of volatile fatty acids in the blood and in the drainage from pancreatic fistula in pancreatic necrosis

2018 ◽  
Vol 11 (4) ◽  
pp. 232-236 ◽  
Author(s):  
Alexander Sergeevich Мetelev ◽  
Eduard S Akayzin ◽  
Alexander K Gagua
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Pancreatic Necrosis ◽  
Capillary Column ◽  
Isovaleric Acid ◽  
Gas Liquid Chromatography ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Healthy Donors

Relevance. The informative value of the content of volatile fatty acids (VFA) in the fistulous drainage from pancreas not been studied in the diagnosis of pancreonecrosis (PN). The informative value of the indices of the content of VFA in the blood has not been studied sufficiently in the diagnosis of PN. Aim of the study is to compare the content of VFA in the blood and in the fistulous drainage from pancreas in patients with pancreatic necrosis. Materials and methods. Samples of blood and fistulous drainage from pancreas isolated from patients with a confirmed diagnosis of PN were studied (n = 18). There was analysis of the concentrations of VFA: acetic, propionic, butyric and isovaleric acids by gas-liquid chromatography on an automated gas chromatograph "Crystallux-4000" with a capillary column "HP-FFAP" Agilent Technologies and a flame ionization detector. The anaerobic index and sum of VFA were calculated. Results. Higher values of the content of acetic, propionic and butyric acids, the sum of VFA and lower values of the content of isovaleric acid and anaerobic index were found in patients with PN in comparison with those of practically healthy donors. Higher values of the content of acetic, propionic and isovaleric acid, the sum of VFA and anaerobic index were noted in the fistulous drainage from pancreas in comparison with the same parameters in blood in patients with PN. The correlation of pairwise conjugated parameters of the content of propionic and isovaleric acids, anaerobic index and the sum of VFA in fistulous drainage from pancreas and blood are discovered in patients with PN. Conclusions. The concentration of acetic, propionic and isovaleric acids, the sum of VFA were higher in the fistulous drainage from pancreas than in the blood in patients with PN. The analysis of the parameters of the VFA can be used as additional criteria for the early diagnosis of PN.

scholarly journals Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 108
Author(s):  
Yichong Wang ◽  
Sijiong Yu ◽  
Yang Li ◽  
Shuang Zhang ◽  
Xiaolong Qi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Acid Content ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Quadratic Response ◽  
In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

DUST-BORNE TRACE GASES AND ODORANTS The analysis of dust-borne trace gases requires their i-solation from the dust particles. Procedures for the isolation and characterization of trace gases and odorants in the dust from pig houses are given by SCHAEFER et al. (29), HAMMOND et al.(30) and TRAVIS and ELLIOTT (31). Alcoholic solvents were found to be effective for the extraction of volatile fatty ac­ ids and phenols from the dust of hen (32) and pig houses (33), (34). Today, gas chromatography is usually used for the sepa­ ration and identification of the trace gases. Table IV gives a literature review of compounds identified in the dust of pig houses. There are only very few reports on investigations on the dust from hen houses (32). Most of the odours coming from livestock production units are associated with the biological degradation of the animal wastes (35), the feed and the body odour of the animals (1). Volatile fatty acids and phenolic compounds were found to con­ tribute mostly to the strong, typical odour of animal houses by the help of sensory evaluations parallel to the chemical analysis (29),(30). Table V gives quantitative values of volatile fatty acids and phenolic/indolic compounds found in the aerosol phase and in settled dust of piggeries, respectively. The results from the aerosol phase coincide, particularly as far as acetic acid is concerned. For the investigations of the settled dust the coefficients of variation (CV) and the relative values (%) characterizing the percentage of the single compounds as part of the total amount are quoted. The values are corrected with the dry matter content of the dust. Main components are acetic acid and p-cresol, respectively. Table VI compares results from air, dust and slurry in­ vestigations on VFA and phenolic/indolic compounds in piggeries. Relative values are used. When comparing the results derived from investigations on dust, air or slurry it is necessary to use relative values because of the different dimensions, for experience shows that in spite of large quantitative differ­ ences between two samples within the group of carboxylic acids and within the group of phenolic/indolic compounds the propor­ tions of the components remain rather stable (36). In the group of VFA acetic acid is the main component in air, dust, and slurry followed by propionic and butyric acid. The other three acids amount to less than 25%. In the group of phenols/ indoles p-cresol is the main component in the four cited in­ vestigations. However, it seems that straw bedding can reduce the p-cresol content; in this case phenol is the main compo­ nent , i nstead (37 ). 4. EMISSION OF DUST-BORNE VFA AND PHENOLS/INDOLES FROM PIGGERIES The investigations of dust from piggeries show that both VFA and phenols/indoles are present in a considerable amount. However, compared to the air-borne emissions calculated on the base of the results of LOGTENBERG and STORK (38) less than the tenth part (1/10) of phenols/indoles and about the hundredth part (1/100) of VFA are emitted by the dust, only. Table VII compares the dust-borne and air-borne emissions of VFA and

1986 ◽  
pp. 337-337
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Volatile Fatty Acids ◽  
Trace Gases ◽  
Matter Content ◽  
Biological Degradation ◽  
Indolic Compounds ◽  
Settled Dust ◽  
Main Components ◽  
Main Component

scholarly journals Determination of N-Nitrosodimethylamine in the Smoke of High-Nitrate Tobacco Cigarettes

1973 ◽  
Vol 7 (2) ◽  
Author(s):  
G.P. Morie ◽  
C.H. Sloan
Keyword(s):  
Capillary Column ◽  
Glass Capillary Column ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Glass Capillary ◽  
Flame Ionization ◽  
Basic Solutions ◽  
Poly Ethylene ◽  
Gas Chromatographic Method

AbstractA gas chromatographic method was developed for the determination of N-nitrosodimethylamine (NDMA) in cigarette smoke. The NDMA in the smoke of 200 cigarettes was trapped in a solution of sodium hydroxide and separated from most of the smoke components by distillation from acidic and basic solutions. The aqueous solution was extracted for 8 hr. into ethyl ether in a Dean Stark apparatus. To concentrate the solution of NDMA, the ether was distilled until only 5 ml of the solution remained. An aliquot of this solution was analysed by means of a gas chromatograph equipped with a 200-ft. glass capillary column coated with Carbowax 20-M poly(ethylene glycoI). An alkali metal flame ionization detector with a selectivity of 10*/1 for nitrogen compounds to normal hydrocarbons was used. Small amounts (2 ng) of nitrosamines in the presence of large amounts of other compounds were easily detected. N-nitrosodimethylamine-C

scholarly journals POTENTIAL OF LIVESTOCK MANURE FOR COAL ACTIVATION

2017 ◽  
Vol 21 (1) ◽  
pp. 26 ◽  
Author(s):  
EllIN HARlIA HARlIA ◽  
MARlINA ET ◽  
MASITA R ◽  
RAHMAH KN
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Acetic Acid ◽  
Volatile Fatty Acids ◽  
Coal Bed ◽  
Coal Bed Methane ◽  
Anaerobic Conditions ◽  
Descriptive Approach ◽  
Carbon Dioxide Co2 ◽  
Reduction Of Co2

The natural methane formed by bacteria in anaerobic conditions is known as biogenic gas. Gas trapped in coal, formed through thermogenesis as well as biogenesisis known as coal-bed methane (CBM). The availability of organic material as decomposition of this material into methane is continuously required for the production of methane in the coal aquifer. The aim of this research was to investigate whether or not cattle feces bacteria were able to grow and produce methane in coal. Parameters measured were Volatile Fatty Acids (VFA) and the production of biogas, such as nitrogen, hydrogen, carbon dioxide, and methane. Explorative method was used and data obtained was analyzed by descriptive approach. The results showed that the bacteria found in the feces survived in the coal and produce biogas. On day 2 when the process was at the acidogenesis phase, it produced VFA with the largest component of acetic acid. Acetic acid would undergo decarboxylation and reduction of CO2 followed by reactions of H2and CO2 to produce methane (CH4) and carbon dioxide (CO2) as the final products. ,

scholarly journals Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Ruiling Gao ◽  
Zifu Li ◽  
Xiaoqin Zhou ◽  
Wenjun Bao ◽  
Shikun Cheng ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Yarrowia Lipolytica ◽  
Butyric Acid ◽  
Lipid Content ◽  
Volatile Fatty Acids ◽  
Lipid Production ◽  
Initial Ph ◽  
Alkaline Conditions

Abstract Background Volatile fatty acids (VFAs) can be effective and promising alternate carbon sources for microbial lipid production by a few oleaginous yeasts. However, the severe inhibitory effect of high-content (> 10 g/L) VFAs on these yeasts has impeded the production of high lipid yields and their large-scale application. Slightly acidic conditions have been commonly adopted because they have been considered favorable to oleaginous yeast cultivation. However, the acidic pH environment further aggravates this inhibition because VFAs appear largely in an undissociated form under this condition. Alkaline conditions likely alleviate the severe inhibition of high-content VFAs by significantly increasing the dissociation degree of VFAs. This hypothesis should be verified through a systematic research. Results The combined effects of high acetic acid concentrations and alkaline conditions on VFA utilization, cell growth, and lipid accumulation of Yarrowia lipolytica were systematically investigated through batch cultures of Y. lipolytica by using high concentrations (30–110 g/L) of acetic acid as a carbon source at an initial pH ranging from 6 to 10. An initial pH of 8 was determined as optimal. The highest biomass and lipid production (37.14 and 10.11 g/L) were obtained with 70 g/L acetic acid, whereas cultures with > 70 g/L acetic acid had decreased biomass and lipid yield due to excessive anion accumulation. Feasibilities on high-content propionic acid, butyric acid, and mixed VFAs were compared and evaluated. Results indicated that YX/S and YL/S of cultures on butyric acid (0.570, 0.144) were comparable with those on acetic acid (0.578, 0.160) under alkaline conditions. The performance on propionic acid was much inferior to that on other acids. Mixed VFAs were more beneficial to fast adaptation and lipid production than single types of VFA. Furthermore, cultures on food waste (FW) and fruit and vegetable waste (FVW) fermentate were carried out and lipid production was effectively improved under this alkaline condition. The highest biomass and lipid production on FW fermentate reached 14.65 g/L (YX/S: 0.414) and 3.20 g/L (YL/S: 0.091) with a lipid content of 21.86%, respectively. By comparison, the highest biomass and lipid production on FVW fermentate were 11.84 g/L (YX/S: 0.534) and 3.08 g/L (YL/S: 0.139), respectively, with a lipid content of 26.02%. Conclusions This study assumed and verified that alkaline conditions (optimal pH 8) could effectively alleviate the lethal effect of high-content VFA on Y. lipolytica and significantly improve biomass and lipid production. These results could provide a new cultivation strategy to achieve simple utilizations of high-content VFAs and increase lipid production. Feasibilities on FW and FVW-derived VFAs were evaluated, and meaningful information was provided for practical applications.

Studies on the production of volatile fatty acids from grass by rumen liquor in an artificial rumen: II. The volatile fatty acid production from dried grass

1957 ◽  
Vol 49 (2) ◽  
pp. 171-179 ◽  
Author(s):  
A. John ◽  
G. Barnett ◽  
R. L. Reid
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Metabolic Pathways ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Water Soluble ◽  
Fatty Acid Production ◽  
Growing Seasons ◽  
Titration Curves

1. A study has been made of the production of volatile fatty acids obtainable from dried grass and its gross water-soluble and water-insoluble separates, in the artificial rumen, over two growing seasons.2. In contradistinction to fresh grass, the dried grass gives a consistent production of acetic acid proportionately greater than propionic acid, at all stages of maturity, but when aqueous extracts of the dried grass, and the resultant extracted grass, respectively, are examined separately in the artificial rumen, it is found that the former yield preponderating amounts of acetic acid while the latter give amounts of propionic acid equal to, or exceeding, the corresponding productions of acetic acid.3. An examination of the titration curves for the total acids obtained from the dried grass, extracted grass and grass extract runs, indicates an approach to an incomplete relationship between the residual carbohydrate in the extracted grass and cellulose, while the grass extract reveals itself as the chief source of acetic acid in the whole dried grass, the acid being formed very speedily at the start of the run.4. The suggested sources and some of the possible metabolic pathways involved in the formation of v.f.a. from grass are discussed in the text.

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