scholarly journals The prophylactic effect of date palm (Phoenix dactylifera L.) fruit extract on testicular toxicity induced by formaldehyde: An experimental study

2020 ◽  
Author(s):  
Mahdieh Zaren ◽  
Tahereh Haghpanah ◽  
Majid Asadi Shekari ◽  
Seyed Hassan Eftekhar-Vaghefi
Keyword(s):  
Date Palm ◽  
Sperm Count ◽  
Antioxidant Properties ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Testicular Toxicity ◽  
Male Mice ◽  
Fruit Extract ◽  
Date Fruit ◽  
Prolonged Contact

Background: Formaldehyde (FA) is one of the most widely used materials in industries and in sciences. Prolonged contact with FA might have harmful effects on fertility due to the increase in the reactive oxygen species level. On the other hand, date palm (Phoenix Dactilifera L.) fruit extract (DPFE) contains a high concentration of natural antioxidants that could scavenge free radicals. Objective: The aim was to investigate the prophylactic effects of DPFE, with strong antioxidant properties, on FA-induced testicular toxicity in male mice. Materials and Methods: Thirty-two adult NMRI male mice with a weight range of 25- 35 gr (9-10 wk old) were randomly divided into four groups: control group (distilled water, orally for 35 days), FA group (FA; 0.25 mg/kg intraperitoneally (i.p.) for 20 days), treatment group (Date (DT) + FA; DPFE, 4 mg/kg for 35 days followed by FA administration, 0.25 mg/kg, i.p., for 20 days), date fruit extract group (DT; DPFE, 4 mg/kg, orally for 35 days). After this, blood was collected and left epididymis and testis tissues were isolated to evaluate the sperm parameters and histological examination, respectively. Results: The FA administration increased the sperm morphological anomalies and reduced the sperm count, viability and motility, and also testosterone compared to the control group (p ≤ 0.001). In addition, histological studies of the testes showed that FA causes changes in the testis seminiferous tubules such as destruction of germinal epithelium and vacuolization of the tubules. The DPFE consumption before FA administration could partially ameliorate the reduced testosterone, sperm, and testicular parameters due to FA. Conclusion: The DPFE use might have discount effects on FA-induced testicular toxicity. Key words: Formaldehyde, Date fruit, Testis, Toxicity, Sperm, Testosterone.

scholarly journals THE EFFECT OF EXTRACT OF DATE PALM FRUIT (PHOENIX DACTYLIFERA L.) ON FERTILITY IN MALE MICE (MUS MUSCULUS L.)

2019 ◽  
Vol 12 (1) ◽  
pp. 418
Author(s):  
Dwisari Dillasamola ◽  
Almahdy A ◽  
Feni Elfianita ◽  
Skunda Diliarosta ◽  
Biomechy Oktomalio P ◽  
...  
Keyword(s):  
Date Palm ◽  
Sperm Quality ◽  
Sperm Count ◽  
Intraperitoneal Administration ◽  
Ethanol Extract ◽  
Control Group ◽  
Testis Weight ◽  
Male Mice ◽  
Palm Fruit ◽  
Date Fruit

Objective: Date palm is one of the plants that have used empirically to increase fertility. Ethanol induction can cause sperm damage in male mice. Sperm damage is one of the infertility agents. The aim of this study was to observe and determine anti-infertility effects of the ethanol extract of date palm fruit in khalal stage in male mice compared with propolis.Methods: A total of 5 groups were assigned to 1 control and 4 experimental groups. Control group was treated by intraperitoneal administration of ethanol only. Meanwhile, experimental groups was treated by intraperitoneal administration of ethanol induction and oral administration of 140, 280, and 560 mg/kg BW of khalal date fruit extract and 1400 mg/kg BW of propolis for 34 days. At 35th day, the mice were deeply anesthetized with anesthetic ether and sacrificed. Laparotomy was performed to collect sperms from epididymis and testes for determining testis weight to analyze the sperm. The data were analyzed using one-way ANOVA and Duncan’s test.Results: The result of the research showed that there were anti-infertility activity of ethanol extract of date palm fruit in khalal stage (p<0.05) on count, motility of sperm and weight of testes in male white mice toghether with larger doses administration. The optimum activity was accepted by the administration of doses 560 mg/kg BW.Conclusion: Ethanol extract of khalal date fruit and propolis can increase the quality of sperm pervade sperm count, sperm motility, and weight of testes. Ethanol extract of khalal date fruit can increase sperm quality higher than propolis.

scholarly journals THE EFFECT OF EXTRACT OF DATE PALM FRUIT (PHOENIX DACTYLIFERA L.) ON FERTILITY IN MALE MICE (MUS MUSCULUS L.)

2019 ◽  
Vol 12 (1) ◽  
pp. 418
Author(s):  
Dwisari Dillasamola ◽  
Almahdy A ◽  
Feni Elfianita ◽  
Skunda Diliarosta ◽  
Biomechy Oktomalio P ◽  
...  
Keyword(s):  
Date Palm ◽  
Sperm Quality ◽  
Sperm Count ◽  
Intraperitoneal Administration ◽  
Ethanol Extract ◽  
Control Group ◽  
Testis Weight ◽  
Male Mice ◽  
Palm Fruit ◽  
Date Fruit

Objective: Date palm is one of the plants that have used empirically to increase fertility. Ethanol induction can cause sperm damage in male mice. Sperm damage is one of the infertility agents. The aim of this study was to observe and determine anti-infertility effects of the ethanol extract of date palm fruit in khalal stage in male mice compared with propolis.Methods: A total of 5 groups were assigned to 1 control and 4 experimental groups. Control group was treated by intraperitoneal administration of ethanol only. Meanwhile, experimental groups was treated by intraperitoneal administration of ethanol induction and oral administration of 140, 280, and 560 mg/kg BW of khalal date fruit extract and 1400 mg/kg BW of propolis for 34 days. At 35th day, the mice were deeply anesthetized with anesthetic ether and sacrificed. Laparotomy was performed to collect sperms from epididymis and testes for determining testis weight to analyze the sperm. The data were analyzed using one-way ANOVA and Duncan’s test.Results: The result of the research showed that there were anti-infertility activity of ethanol extract of date palm fruit in khalal stage (p<0.05) on count, motility of sperm and weight of testes in male white mice toghether with larger doses administration. The optimum activity was accepted by the administration of doses 560 mg/kg BW.Conclusion: Ethanol extract of khalal date fruit and propolis can increase the quality of sperm pervade sperm count, sperm motility, and weight of testes. Ethanol extract of khalal date fruit can increase sperm quality higher than propolis.

scholarly journals Low semen quality and adverse histological changes in testes of adult male mice treated with bee venom (Apis mellifera)

2021 ◽  
Vol 11 (1) ◽  
pp. 70-79
Author(s):  
Sassia O. Regeai ◽  
Salma A. Abusrer ◽  
Naema S. Shibani
Keyword(s):  
Male Infertility ◽  
Adult Male ◽  
Semen Quality ◽  
Sperm Count ◽  
Bee Venom ◽  
Reproductive Status ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Histological Changes

Background: Male infertility has been on the rise since the past seven decades. Recently, in Libya, bee venom therapy (BVT) has become a popular method among alternative healthcare practitioners for treating male infertility. However, a literature search did not find any published studies that investigated the use of BVT for infertility treatment. Aim: To investigate the effect of bee venom on the male reproductive status through measurements of semen quality parameters and testicular histological changes in adult male mice. Methods: A total of 48 male mice were randomly divided into three experimental groups (which were subdivided into two subgroups with eight mice each) as follows: control, bee venom sting (BVS), and bee venom injection (BVI). The normal control subgroup mice were not subjected to any treatment, while the vehicle control subgroup mice were injected (i.p.) with 200 μl of 0.9% saline solution. In the BVS-treated subgroups, each mouse was stung by one live bee for five times (BVS-5) or seven times (BVS-7) every third day for 2 or 3 weeks. While each mouse in the BVI-treated subgroups received 23 μg/kg in a dose volume of 200 μl BVIs (i.p.) for five times (BVI-5) or seven times (BVI-7) every third day for 15 or 21 days. Results: The findings of this study showed that repeated bee venom treatment by sting or injection to adult male mice resulted in a significant decline in testosterone levels, sperm count, sperm motility, and a very significant increase in the percentage of abnormal sperm morphology; also, there were harmful testicular histological changes in the structural organization of seminiferous tubules and degenerative changes in the germinal epithelium compared to control group. Conclusion: The results of this study provide evidence for the low semen quality and adverse testicular histological changes in male mice treated with bee venom. Hence, there is a desperate need for educating alternative healthcare practitioners and infertile couples about the harmful effects of BVT on reproductive status.

scholarly journals Alcohol Consumption and Its Effect on Testicular Structure and on Sperm Count and Motility in Parent Mice and Their Offspring

2013 ◽  
Vol 12 (1) ◽  
Author(s):  
Cinaria T. Albadri
Keyword(s):  
Alcohol Consumption ◽  
Sperm Count ◽  
Male Offspring ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Ethanol Treatment ◽  
Male Mice ◽  
Female Mice ◽  
Group 2 ◽  
Group 1

Introduction: The goal of the present study was to examine the effect of alcohol consumption on sperm count and motility and the morphological changes in the seminiferous tubules of parent mice and their offspring. Methods: Animals were divided into two groups, Group 1 (alcohol group) of twelve male and twelve female mice, were given a daily dose of (3 g/kg body weight as 25%, v/v) ethanol by gastric gavage for four and eight weeks. Group 2 (control group) also of twelve male and twelve female mice; received normal access of food and water. After four weeks of treatment, the males and females in each group were allowed to mate, and ethanol treatment continued for up to another four weeks. Twelve male offspring from group 1 and twelve male offspring from group 2 were selected randomly and allowed to become mature. Male parent mice were killed at the 4th and 8th weeks of treatment, and their male offsprings were killed when they reached maturity age. Results: Physiological examination of the sperm solution showed that there was a significant decrease in sperm count and motility after 4 and 8 weeks of ethanol treatment in parent male mice, but this decrease was not significant in their adult offspring. Furthermore, histological investigations indicated testicular lesions in the parent male mice and their adult male offspring. Conclusion: Alcohol abuse has deleterious effects on the testes structure and on the sperm count and motility of the epididymal spermatozoa of both parent mice and their offspring.

scholarly journals PENGARUH SARI KURMA TERHADAP PROLAKTIN DAN PENGELUARAN ASI PADA IBU POSTPARTUM DI BPM PIPIN HERIYANTI YOGYAKARTA TAHUN 2017

2019 ◽  
Vol 6 (3) ◽  
pp. 195-198
Author(s):  
Dwi Yulinda ◽  
Imroatul Azizah
Keyword(s):  
Treatment Group ◽  
Breast Milk ◽  
Breast Feeding ◽  
Date Palm ◽  
Antioxidant Properties ◽  
Control Group ◽  
Feeding Rates ◽  
Palm Fruit ◽  
Exclusive Breast Feeding ◽  
Palm Fruits

Background: Breastfeeding is exceptionally superior, giving mother and infants distinct and subtastial physical, mental, and developmental health advantages. Palm fruit has content of phytonutrients with antioxidant properties and galactagogues to help increase their milk supply in the early postpatum days. The lowest exclusive brest feeding rates in DIY is in the city of Yogyakarta. Exclusive breast feeding improves infant immunity so as to minimize the occurrence of diarrhea, constipation, fever that is caused by some allergic reactions. Objectifive: :This study aimed to analyze the relationship between date palm fruits on postpartum to prolactin and volume of breast milk Method: Research was conducted on maternal postpartum which is divided into two groups. They are the control group; and the treatment group which was given dates palm fruit. Breast milk volume and prolactin was measured at 3 day after labor. The analytical method used is Student T-test. Results: Date Palm fruits increased prolactin and volume of breast milk which was significantly on firstweek breastfeeding increase in the treatment group compared to the control group at p <0.05. Conclusion: Date Palm fruits is one of the alternatives that can be done to improve the success of exclusive breast feeding on postpartum. Key Words: Date palm fruit, postpartum, prolactin and volume of breast milk

scholarly journals The Effects of Vasectomy on Testicular Tissue of Mice: Histological Changes and DNA Fragmentation Study

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Salman MO ◽  
Al-Wasiti EA ◽  
Thamir KA ◽  
Al-Ani IM ◽  
Al-Salihi AR
Keyword(s):  
Single Cell ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sham Operation ◽  
Male Mice ◽  
Histological Changes ◽  
Single Cell Gel Electrophoresis

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.

scholarly journals Dichloroacetic acid-induced testicular toxicity in male rats and the protective effect of date fruit extract

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Amira El Arem ◽  
Lamia Lahouar ◽  
Emna Behija Saafi ◽  
Amira Thouri ◽  
Fatma Ghrairi ◽  
...  
Keyword(s):  
Protective Effect ◽  
Dichloroacetic Acid ◽  
Male Rats ◽  
Testicular Toxicity ◽  
Fruit Extract ◽  
Date Fruit

Endocrine disrupting potential and reproductive dysfunction in male mice exposed to deltamethrin

2016 ◽  
Vol 36 (3) ◽  
pp. 218-226 ◽  
Author(s):  
A Ben Slima ◽  
Y Chtourou ◽  
M Barkallah ◽  
H Fetoui ◽  
T Boudawara ◽  
...  
Keyword(s):  
Pesticide Exposure ◽  
Semen Quality ◽  
Sperm Quality ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Cell Cytoplasm ◽  
Pregnant Females ◽  
Sperm Density ◽  
Endocrine Disrupting

Pesticide exposure may affect semen quality and male fertility in humans. The aim of the present work was to elucidate the adverse effects of deltamethrin (Delta), a synthetic pyrethroid, on exposed male mice and their offspring. Adult male Albino/Swiss mice received deltamethrin (5 mg/kg) daily for 35 days and mated with untreated females to produce offspring. Classical measurements of ejaculate and sperm quality and testicular histopathological changes were assessed. Deltamethrin treatment affects sperm quality and quantity in the ejaculated semen of mice that had also markedly impaired libido as measured by indices of mating and fertility and number of pregnant females housed with male mice exposed to this pesticide. Exposure mice to deltamethrin significantly decreased their testosterone and inhibin B levels and affected reproductive performance. Testes of exposed mice showed marked histopathological alterations as compared to the control group. The mice exposed to 5 mg/kg body weight/day of deltamethrin showed severe alterations of the seminiferous tubules, sloughing of the germ cells, the vacuolization of germ cell cytoplasm, and the disruption of spermatogenic cells compared to the control group. Altered pregnancy outcomes were directly attributed to damage of sperm of male mice exposed to deltamethrin compared to the control group. We concluded that exposure to deltamethrin affected the reproductive system of male mice explored by altered total sperm density, motility, and morphology in mice spermatozoa.

scholarly journals Testicular Morphological Changes Produced by Fluoroquinolones in Adult Male Albino Rats

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
Rubina Iqbal ◽  
Saud Iqbal ◽  
Iram Atta
Keyword(s):  
Adult Male ◽  
Leydig Cells ◽  
Morphological Changes ◽  
Sperm Count ◽  
Research Work ◽  
Testicular Tissue ◽  
Reproductive Organs ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats

AbstractObjectives:  The objective of this research work was to observe the testicular morphological changes produced by fluoroquinolones in the reproductive organs of adult male albino rats, and to see whether these changes are reversible after discontinuation of the drugs.Materials and Method:  Eighty adult male albino rats weighing 200 – 300 gms were randomly selected and divided into four groups i.e. A, B, C & D, having 20 animals in each group. A, B & C, were the experimental groups & D served as control group. All the groups were further divided into sub groups 1 & 2. Three fluoroquinolones i.e. Ciprofloxacin (135 mg / kg / day), Ofloxacin (75 mg / kg / day) & Enoxacin (12.5 mg / kg/ day) were given to the groups A, B & C respectively for 42 days. Animals of group D received dis-tilled water only. Animals of sub groups A1, B1, C1 &D1 were sacrificed on 42nd day and testicular tissue was obtained for morphological study. Animals of subgroups A2, B2, C2 & D2 were sacrificed on 84th day and testicular tissue for morphological changes was taken. No of leydig cells, height of epithelium and diameter of seminiferous tubules were taken as experimental parameters for morphological changes.Results:  The study indicated statistically significant (P < 0.05) decrease in height of epithelium, diameter of seminiferous tubules and no. of leydig cells in experimental groups as compared to the control groups.Conclusion:  The changes observed in morphology could lead to decrease in sperm count and testosterone levels. This study suggests gonadotoxic potentials of fluoroquinolones and adds concern to the indiscriminate and widespread use of fluoroquinolones and recommends more rational use of these drugs.

scholarly journals A preliminary study on the reproductive toxicity of GS-5734 on male mice

2020 ◽  
Author(s):  
Jing Fan ◽  
Jiao Luo ◽  
Depeng Zhao ◽  
Tianqin Deng ◽  
Yuanbo Weng ◽  
...  
Keyword(s):  
Sperm Count ◽  
Reproductive Toxicity ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Control Group ◽  
Male Mice ◽  
Abnormal Sperm ◽  
Daily Dose ◽  
Preliminary Study ◽  
Group 1

AbstractBackgroundGS-5734 as a novel and promising medicine for COVID-2019, its biological impact on the mammalian reproductive system has not been systematically studied. The aim of this study was to evaluate the effects of GS-5734 on sperm parameters and spermatogenesis in mice.Materials and MethodsIn this study, GS-5734 was synthesized according to the report. 28 adult male mice were randomly segregated into four groups (n=7 for each group). The group 1 was set as the control group, the group 1, 2, 3 and 4 were administered with GS-5734 at a daily dose of 0, 10, 50, 150 μg/mouse respectively, by intraperitoneal injection for 10 days. On the 7th day after the last injection, the testes and cauda epididymides were collected for HE staining and sperm concentration, motility, morphology analysis.ResultsThe results indicated that after treated with GS-5734, the total sperm count and motile sperm rate showed downward trends, the abnormal sperm rate showed an increasing trend. As compared with the control group, GS-5734 at a daily dose of 150 μg/mouse caused a significant decrease in sperm concentration and motility, and a significant increased of abnormal sperm rate; the 50 μg/mouse drug treatment lead to a significant decrease in sperm motility and an increase in abnormal sperm rate. The HE staining of testicular and epididymal tissues showed that the spermatogenesis of mice was significantly deteriorated with the increasing dosage of GS-5734, especially in the 150 μg/mouse group.ConclusionOur findings suggest that a high dosage of GS-5734 may induce testicular toxicity and result in deterioration of sperm parameters in mice. More investigation on the reproductive toxicity of GS-5734 is required.

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