scholarly journals CHARACTERISTICS OF ACTINOBACTERIA FROM MYTILUS GALLOPROVINCIALIS OF ODESSA GULF OF THE BLACK SEA

2021 ◽  
pp. 84-93
Author(s):  
N. V. Korotaeva ◽  
I. V. Strashnova ◽  
N. Yu. Vasylieva ◽  
K. S. Potapenko ◽  
I. P. Metelitsina ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Black Sea ◽  
Mytilus Galloprovincialis ◽  
Biochemical Properties ◽  
Biologically Active ◽  
The Black Sea ◽  
Identification System ◽  
Streptomyces Sp ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

Today both academic and commercial interests in marine actinobacteria are growing. As they live in a unique environment that promotes the synthesis of new biologically active metabolites. The aim of the work is isolation, primary identification and study of morphological, cultural, physiological and biochemical properties of actinobacteria, separate from mussels (Mytilus galloprovincialis) of the Odessa Bay of the Black Sea. Methods. Samples of mussels collected in the coastal zone of Odessa Bay were used as material for the isolation of actinobacteria. Isolation of actinobacteria and the study of their morphological, cultural, physiological and biochemical properties was carried out by traditional microbiological methods. The fatty acid composition was determined on an Agilent 7890 semi-ionization gas chromatograph (Agilent Technologies, USA) to identify test strains using the Sherlock Microbial Identification System library. Results. From samples of mussels collected in 2020 in the area of the Hydrobiological Station of Odessa National I.I. Mechnikov University were isolated 14 strains of actinobacteria. They were identified by fatty acid analysis to Streptomyces genus. Strains of Streptomyces sp. Myt2, Myt6 and Myt7ch synthesized melanoid pigments. Strains of actinobacteria are well absorbed by most of the studied sources of carbon, except strains of Streptomyces sp. Myt12a, Myt12b. Almost half of the strains have oxidase activity and coagulate milk. Conclusions. As a result of microbiological studies for the first time were characterized morphological, cultural and physiological and biochemical properties and determined taxonomic composition by fatty acid spectra for actinobacteria isolated from the mussels of the Odessa Bay of the Black Sea.

scholarly journals THE COMPOSITION OF CELLULAR FATTY ACIDS OF ACTINOBACTERIA FROM THE SURFACES OF BIOLOGICAL GROWTH OF THE ODESA GULF OF THE BLACK SEA

2021 ◽  
pp. 60-70
Author(s):  
N. V. Korotaeva ◽  
K. S. Potapenko ◽  
I. V. Strashnova ◽  
I.P. Metelitsyna ◽  
V. O. Ivanytsia
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Black Sea ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
The Black Sea ◽  
Identification System ◽  
Fatty Acid Profiles ◽  
Genus Streptomyces ◽  
Biological Growth

Aim. Determination of fatty acid composition of actinobacteria isolated from the surfaces of biological growth of the Odesa gulf of the Black Sea, and their identification. Methods. The 31 isolated strains of actinobacteria were grown in TSB at 28 ° C and 150 rpm for 72 hours. Fatty acid methyl esters of the studied strains were determined according to the MIS Operating Manual on a gas chromatograph Agilent 7890, identification was performed using the identification system of microorganisms MIDI Sherlock. Results. Using chromatographic analysis of fatty acids, it was found that of the 27 studied strains of actinobacteria were identified to the genus Streptomyces, and the 4 strains - to the genus Nocardiopsis. It was found that the fatty acid profiles of the studied actinobacteria of the genus Nocardiopsis were dominated by fatty acids: 15:0 ANTEISO, 16:0 ISO, 17:0 ANTEISO, 18:1 CIS 9, and the fatty acid profiles of bacteria of the genus Streptomyces - 14:0 ISO, 15:0 ANTEISO, 16:0 ISO, 17:0 ANTEISO. Conclusions. Actinobacteria the surfaces of biological growth of the Odesa gulf of the Black Sea belong to the genera Streptomyces and Nocardiopsis, and their fatty acid profiles are characterized by the dominance of isomers of branched saturated fatty acids.

scholarly journals Some Physiological and Biochemical Properties of Microalgae in Vegetable Soil

2011 ◽  
pp. 86-89
Author(s):  
N Buyankhishig ◽  
Ch Zultsetseg ◽  
B Odgerel
Keyword(s):  
Essential Amino Acids ◽  
Biochemical Properties ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Unicellular Alga ◽  
Vegetable Soil ◽  
Academy Of Sciences ◽  
Physiological And Biochemical ◽  
Active Substances ◽  
Physiological And Biochemical Properties

Chlorella is included in a group of an autotrophic protococci alga and is occurred in ponds, wells, moist soil and on the surface of trees. It is circular in shape, 1.5-10.5μm in size and is a unicellular alga (1). Among them, some strains of Chlorella genus contain 45-50% protein, 20- 25% carbohydrate, 7-10% lipids, and biologically active substances such as several types of vitamins and essential amino acids, hence, other countries are widely utilizing them in food, forage, pharmaceutical and cosmetic purposes (1.7). Other countries are searching for chances to isolate a microalgae strain from their natural resources which can synthesize biologically active substances, to determine their properties, to utilize them practically and to produce their biopreparations by biotechnological methods (1.2.7). Therefore, we studied the physiological and biochemical properties of the cultures that were isolated from the soil of a greenhouse in order to detect a biologically active microalgae strain.DOI: http://dx.doi.org/10.5564/pmas.v0i4.51 Proceedings of the Mongolian Academy of Sciences 2009 No.4 pp.86-89

scholarly journals Active Antibiotics Production by Actinomycetes Indigenous To Saudi Arabia Soils

2021 ◽  
Vol 11 (6) ◽  
pp. 20-29
Author(s):  
Fetoon M ◽  
helaiwi Alk ◽  
Ismet Ara ◽  
Nadine Moubayed
Keyword(s):  
Saudi Arabia ◽  
Biochemical Properties ◽  
Soil Samples ◽  
Fungal Strain ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Desert Soils ◽  
Growth Environment ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

Streptomyces are the most popular among the Actinomycetes groups and found in soils worldwide. They form an important part of the soil ecology within the Actinomycetales order. Streptomyces are diverse as secondary antibiotic metabolites such as Novobiocin, Amphotericin, Vancomycin, Neomycin, Gentamicin, Chloramphenicol, Tetracycline, Erythromycin and Nystatin. Thus, the current study was aimed to isolate, identify and assess the active antibiotic metabolites produced by different actinomyces sp. found in Saudi Arabian soils. Six samples were collected from desert soils of the Al Thumamah area and analyzed using GS-MS. Scanning Electron Microscopy was used to identify the bacterial strains along with their antibiotic metabolites effectiveness of secondary metabolites (antibiotics) against different Gram-positive (Bacillus subtilis, Staphylococcus aureus), negative pathogens (Pseudomonas aeruginosa, Escherichia coli, Salmonella suis, and Shigella sonnei) as well as the fungal strain Candida albicans was investigated. Thirty active bacterial (F1-30) strains were isolated from the soil samples and the strains F3, F7, F22, F30 have white, gray, pink, yellow and red colours respectively. Only ten strains (F13, F14, F15, F16, FI7, F18, F19, F20, F21, and F22) were found to have antimicrobial activity against at least one pathogen. The optimum growth environment was pH 4-10, temperature (300C), and NaCl (7% w/v) concentration. According to our findings, the extreme desert environment of Al Thumamah from Saudi Arabia is rich in its actinobacterial population with diverse colouring groups and various physiological and biochemical properties. This shows it’s capability of generating secondary metabolite elements that could inhibit pathogenic microorganisms.

scholarly journals DEVELOPMENT OF THE BLACK SEA HYDROBIONT RESOURCE STUDY METHOD AS A NEW ECONOMICALLY FEASIBLE DIRECTION OF PHARMACOLOGY AIMING TOWARD SAVE HUMAN HEALTH AND ECOLOGY

2021 ◽  
Vol 941 (1) ◽  
pp. 012023
Author(s):  
O P Chzhu ◽  
D E Araviashvili ◽  
A L Tumanova
Keyword(s):  
Black Sea ◽  
Extraction Method ◽  
Raw Materials ◽  
Aquatic Organisms ◽  
Biologically Active ◽  
The Black Sea ◽  
Phase Extraction ◽  
Biochemical Activity ◽  
Two Phase ◽  
Active Substances

Abstract The selection of the Black Sea aquatic area as a hydrobiont biologically active compound source allows resolving several environmental problems including development of an integrated hydrobiological resource management system for the Black Sea as a new sector of modern pharmacology. Aquatic organisms produce unique secondary metabolites. This paper presents the results of studies on the development of the biologically active substance extraction technology from non-commercial aquatic organisms as well as a preliminary assessment of the biochemical activity of the substrates obtained. Biologically active substances were extracted using the of two-phase extraction method in conjunction with ultrasound. For the substrates, the antioxidant activity was determined utilising the method that allow conducting screening of pharmaceutical raw materials and biologically active substances with high antioxidant activity. The protective activity evaluation was carried out during the study of the enzymatic alcoholic fermentation kinetics within a self-contained system. The evaluation of lymphocytes proliferative activities influenced by the obtained substrates was carried out using the cultivation method. The acquired data allows recommending the two-phase extraction method combined with ultrasonic voiceover as the effective one when processing analogical marine raw materials. The isolated substrates are characterised by a pronounced biochemical activity in relation to the living systems cells, which suggests a significant synergistic effect with derivatives of the microalgae Chlorella Vulgaris.

Sign in / Sign up

Export Citation Format

Share Document