scholarly journals DEVELOPMENT OF THE BLACK SEA HYDROBIONT RESOURCE STUDY METHOD AS A NEW ECONOMICALLY FEASIBLE DIRECTION OF PHARMACOLOGY AIMING TOWARD SAVE HUMAN HEALTH AND ECOLOGY

2021 ◽  
Vol 941 (1) ◽  
pp. 012023
Author(s):  
O P Chzhu ◽  
D E Araviashvili ◽  
A L Tumanova
Keyword(s):  
Black Sea ◽  
Extraction Method ◽  
Raw Materials ◽  
Aquatic Organisms ◽  
Biologically Active ◽  
The Black Sea ◽  
Phase Extraction ◽  
Biochemical Activity ◽  
Two Phase ◽  
Active Substances

Abstract The selection of the Black Sea aquatic area as a hydrobiont biologically active compound source allows resolving several environmental problems including development of an integrated hydrobiological resource management system for the Black Sea as a new sector of modern pharmacology. Aquatic organisms produce unique secondary metabolites. This paper presents the results of studies on the development of the biologically active substance extraction technology from non-commercial aquatic organisms as well as a preliminary assessment of the biochemical activity of the substrates obtained. Biologically active substances were extracted using the of two-phase extraction method in conjunction with ultrasound. For the substrates, the antioxidant activity was determined utilising the method that allow conducting screening of pharmaceutical raw materials and biologically active substances with high antioxidant activity. The protective activity evaluation was carried out during the study of the enzymatic alcoholic fermentation kinetics within a self-contained system. The evaluation of lymphocytes proliferative activities influenced by the obtained substrates was carried out using the cultivation method. The acquired data allows recommending the two-phase extraction method combined with ultrasonic voiceover as the effective one when processing analogical marine raw materials. The isolated substrates are characterised by a pronounced biochemical activity in relation to the living systems cells, which suggests a significant synergistic effect with derivatives of the microalgae Chlorella Vulgaris.

scholarly journals Development of beverage recipes with specified bio-corrective properties based on plant raw materials with the ultrasonic extraction method

2020 ◽  
Vol 82 (3) ◽  
pp. 189-199
Author(s):  
M. V. Manukovskaya ◽  
I. P. Shchetilina ◽  
A. G. Kudryakova ◽  
S. R. Kozlova ◽  
A. O. Torosyan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Human Body ◽  
Extraction Method ◽  
Raw Materials ◽  
Ultrasonic Extraction ◽  
Biologically Active ◽  
Plant Raw Materials ◽  
Organoleptic Characteristics ◽  
Active Substances

The development of targeted beverages is a priority for healthy eating. Plant raw materials containing a wide variety of biologically active substances are used for their production. The presence of these ingredients helps to improve many physiological processes in the body, as well as to increase its immune status. The high efficiency of ultrasonic effects on various technological processes was confirmed by numerous studies. Ultrasonic extraction was carried out at t = 20 ? and an ultrasonic wave frequency of 22 kHz. As a result of the research, it was found that the equilibrium state is achieved within 15 minutes with ultrasound exposure. Natural raw materials, their chemical composition and the influence on the human body were studied in the work to develop new beverages recipes. The selected ingredients in a certain quantitative ratio made it possible to obtain beverages with improved bio-corrective properties, which allows satisfying the daily requirement of the human body for biologically active substances. Ultrasonic exposure has a positive effect on the organoleptic characteristics of the beverage and the aftertaste, as proved by the comparative organoleptic evaluation of the samples. The functional properties of the beverages developed were confirmed experimentally. The following was determined in the beverages: macronutrients content, antioxidant activity, vitamin C content, shelf life duration. The ultrasonic extraction method application in the technology of beverages manufacturing allows: to reduce the preparation time by half, to reduce the amount of raw materials by 15%, to increase the content of macronutrients by 45%, to increase the content of vitamin C by 35%, to increase the antioxidant activity by 45-50%, to increase storage stability by 50-60%, to improve organoleptic characteristics. The results obtained prove the intensifying effect of ultrasonic exposure in the production of functional beverages.

scholarly journals Extraction of Biologically Active Substances from Matricaria Chamomilla Flowers

2020 ◽  
Author(s):  
И.Ю. Постраш ◽  
Ю.Г. Соболева ◽  
В.С. Андрущенко
Keyword(s):  
Extraction Process ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Chemical Compositions ◽  
Phase Extraction ◽  
Matricaria Chamomilla ◽  
Two Phase ◽  
Plant Materials ◽  
Active Substances

Важнейшим природным источником лекарственных средств являются лекарственные растения (ЛР), которые содержат разнообразные биологически активные вещества (БАВ). Основными методами извлечения БАВ из ЛР являются методы экстракции, которые приводят к накоплению необходимых биологически важных компонентов в подходящем растворителе (экстрагенте). В зависимости от природы экстрагента, его количества, времени экстракции, температуры и других факторов из одного и того же сырья могут быть получены композиции БАВ с разным химическим составом. Изучено влияние природы экстрагента на степень извлечения гидрофильных и липофильных БАВ из лекарственного растительного сырья на примере цветков ромашки аптечной. В качестве экстрагентов использовалась вода очищенная, растворы этанола различной концентрации, двухфазные системы экстрагентов (смеси растительного масла и водных растворов этанола различных концентраций). В качестве маркеров гидрофильных веществ выступали флавоноиды, в качестве маркеров липофильных соединений – хлорофилл. Установлено, что одним из способов оптимизации процесса экстракции БАВ из цветков ромашки аптечной является применение двухфазной системы экстрагентов, состоящей из подсолнечного масла и 70% этанола, взятых в равных объёмах, которое позволяет осуществить одновременную экстракцию как липофильных, так и гидрофильных компонентов в максимальной концентрации. The most important natural source of medicines is medicinal plants (MP) which contain various biologically active substances (BAS). The main methods for extracting BAS from MP are extraction methods which lead to the accumulation of the necessary biologically important components in a suitable solvent (extraction fluid). Depending on the nature of the extraction fluid, its amount, extraction time, temperature and other factors BAS compositions with different chemical compositions can be obtained from the same raw material. The influence of the nature of the extraction fluid on the recovery rate of hydrophil and lipophilic biologically active substances from medicinal plant materials was studied as exemplified by Matricaria Chamomilla flowers. Purified water, ethanol solutions of various concentrations and two-phase extraction fluid systems (mixtures of vegetable oil and proof spirit of various concentrations) were used as extraction fluids. Flavonoids acted as markers of hydrophil substances, chlorophyll acted as markers of lipophilic compounds. It was found that one of the ways to optimize the extraction process of biologically active substances from Matricaria Chamomilla flowers is the use of a two-phase extraction fluids system consisting of sunflower oil and 70% ethanol taken in equal volumes which achieves simultaneous extraction of both lipophilic and hydrophil components in the maximum concentration.

scholarly journals Studying Properties of Prospective Biologically Active Extracts from Marine Hydrobionts

2020 ◽  
Vol 4 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Olga P. Chzhu ◽  
Daredzhan E. Araviashvili ◽  
Irina G. Danilova
Keyword(s):  
Black Sea ◽  
Marine Invertebrate ◽  
Biological Properties ◽  
Biologically Active ◽  
Phase Extraction ◽  
Two Phase ◽  
Liposomal Drug ◽  
Sulfuric Acid Medium ◽  
Treatment And Prevention ◽  
Alcohol Water

Biologically active substances (BAS) extracted from marine hydrobionts are characterized by high diversity and efficiency. They are represented by carotenoids, phospholipids, saponins and other compounds possessing high antioxidant (AOA), antitumor, immunomodulatory, radioprotective, and hypolipidemic activities. Because of extremely high marine BAS activity, the BAS dose necessary for treatment and prevention of many diseases is very small.  The aim of present work was to assess biological properties of BAS complexes extracted from Black Sea marine inverterbrates for their following using in pharmacological preparations including liposomal drug delivery. BAS complexes were extracted from marine invertebrate tissues by two-phase extraction in combination with ultrasonication. Antioxidant activity of BAS extracts was determined with potassium permanganate discoloration method in an aqueous sulfuric acid medium. Antimicrobial activity was investigated by germination of BAS extracts.  BAS influence on lymphocyte proliferative activity was studied with help of cytogenetic analysis. In this paper, the assessment of antioxidant, antimicrobial and mitogenic activities of biologically active complexes extracted from marine invertebrate tissues is presented. BAS properties in the oil  and alcohol-water phases are compared. For the first time BAS complexes from Black Sea invertebrates are extracted by the resource-light method of two-phase extraction in combination with ultrasonication. Conditions for obtaining BAS extracts with optimal characteristics are proposed.

scholarly journals Choice of Extraction Method for Receiving Extraction from Seeds of Payne Hay with the High Content of Biologically Active Substances

2019 ◽  
Vol 8 (3) ◽  
pp. 35-39 ◽  
Author(s):  
S. S. Belokurov ◽  
E. V. Flysyuk ◽  
I. E. Smekhova
Keyword(s):  
Extraction Method ◽  
Raw Materials ◽  
Ultrasonic Waves ◽  
Ultrasonic Extraction ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
High Yield ◽  
Plant Materials ◽  
Extractive Substances ◽  
Active Substances

Introduction. According to the literature, modern technologies, in particular, extraction in an ultrasonic field, make it possible to obtain concentrates of biologically active substances (BAS) with almost complete preservation of the chemical composition inherent in natural raw materials and high yield of extractive substances. At the same time, the ability to regulate the concentration of recoverable active substances during the technological process opens up prospects for the use of natural components as the main pharmaceutical substance.Aim. The purpose of this study was to select the optimal extraction method for obtaining extracts with a high content of BAS from fenugreek seeds. Materials and methods. Extracts were obtained at a temperature of 60±2 °С, by the method of dynamic maceration using an ultrasonic device. Ultrasonic extraction was performed using an ultrasonic installation I100-6/4. In both cases, purified water and ethanol solutions in various concentrations from 40 to 90% were used as extractants. The ratio of raw materials: extractant was as 1:10 (by weight). A portion of vegetable raw materials (10 g) was placed in a glass and poured 100 cm3 of extractant. Next, maceration or sonication was performed. Maceration was performed with magnetic stirring, the rotation speed was 100 rpm. Extraction was carried out at a temperature of 55–60 °C for 5 hours. Ultrasonic effects on solid plant materials were carried out with an intensity in the range from 17 to 22 kHz for 30–60 minutes.Results and discussion. Studies have allowed to determine the amount of extractives in the seeds of fenugreek hay, and choose the most promising method of extraction to obtain extracts with a high content of biologically active substances. A comparison was made between dynamic maceration and ultrasonic extraction. The most promising was the method of ultrasonic extraction at an oscillation frequency of 22 kHz. For 1 hour of extraction, it was possible to achieve depletion of raw materials. It is established that the content of extractable substances directly proportional depends on the duration of treatment and the frequency of ultrasonic waves. The correlation coefficient was 0.78.Conclusion. Ultrasonic extraction when exposed for 60 minutes allows you to get a greater amount of extractives compared with dynamic maceration. To achieve the same level of extractive substances by the method of dynamic maceration, the raw materials must be processed within 5 hours.

COMPARATIVE ANALYSIS OF BIOACTIVE SUBSTANCES CONTENT IN THE LEAVES AND FRUITS OF A ROWAN-TREE (SORBUS AUCUPARIA FRUCTUS) AND ASSESSMENT OF PROSPECTS IN MEDICINE AND PHARMACY

2020 ◽  
pp. 58-62
Author(s):  
Chernichkina A.D.
Keyword(s):  
Raw Materials ◽  
Wide Distribution ◽  
Biologically Active ◽  
Bioactive Substances ◽  
Plant Raw Materials ◽  
Mountain Ash ◽  
The Russian Federation ◽  
Materials Used ◽  
Active Substances ◽  
Pectin Substances

A large number of biologically active substances, organic acids, tannins, and pectin substances were detected during the study of fruits, leaves, and pulp of the fruits of mountain Ash. The content of these substances in leaves and pulp will significantly expand the arsenal of medicinal plant raw materials used. Given the wide distribution of mountain Ash in the territory of the Russian Federation, harvesting leaves from the same plants after harvesting the fruit and using pulp will make it possible to obtain new phytopreparations.

CONSUMER PROPERTIES OF PASTA WITH POWDER FROM MARIGOLD INFLORESCENCES (TAGETES L.)

2021 ◽  
Vol 40 (4) ◽  
pp. 102-112
Author(s):  
SHAPOVALOVA Nataliia ◽  
VEZHLIVTSEVA Svitlana ◽  
ANTIUSHKO Dmytro
Keyword(s):  
Raw Materials ◽  
Experimental Studies ◽  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Organoleptic Properties ◽  
Biological Value ◽  
Plant Raw Materials ◽  
Active Substances

Background. The deficiency of essential nutrients in the human body is the root cause of reduced efficiency, general resistance of the body to various diseases. That is an urgent problem in the context of the spread of coronavirus infection. In this regard, the search for opportunities to meet the needs of the population in food products that contain biologically active substances (BAS) through the use of high-value and at the same time available plant raw materials is extremely important. Marigolds attract special attention among such raw materials. The aim of the work is to study the chemical composition and evaluate the consumer properties of Tagetes L. varieties, zoned in the Kiev region, to prove the possibility of increasing the biological value of pasta by adding to their recipe powder from marigold inflorescences. Materials and methods. The object of research is pasta of B group (noodles). Inflorescences of marigolds of different varieties gathered during the flowering period in July-September 2019–2020 in the territory of Vasylkiv and Obukhiv districts of Kyiv region, Ukraine were used as enriching plant raw materials. The quality of finished pasta was assessed by organoleptic and physicochemical indexes (moisture content and titratable acidity). The content of mineral elements in the fine powder of marigold inflorescences was determined on the EXHERT-3L device, the quantitative composition and BAS identification – by spectrophotometric analysis on the device Specord-200 Analytic Jena UV-vis. The integrated quality index of pasta products was calculated taking into account the importance factors of individual indexes. Results. Comparing the obtained results, we can state that all the studied varieties – Gold Kopfen, Orange Flame, Hawaii, Equinox are characterized by high biological value, regardless of the area of collection. As a result of calculated and experimental studies it was determined that the addition of 7 % of the supplement does not provide the required supply of vitamins and minerals. Replacing the flour in the recipe by 20 % significantly improves the mineral composition, but deteriorates the taste and aromatic properties of pasta. The most optimal and rational amount of added additive is 15 %, as it improves the organoleptic properties of pasta and enriches their BAS. Conclusion. The usage of non-traditional vegetable raw materials, in particular powder from marigold inflorescences, which is rich in biologically active substances, allows not only to expand the range, improve the quality of finished products, but also increase the biological value of the product. The optimal amount of additive –powder from marigold inflorescences, was defined. It is 15 % by weight of flour in the production of noodles. This concentration allows not only to improve the organoleptic properties, but also to increase the content of biologically active substances – vitamins, micro- and macronutrients. The usage of non-traditional natural raw materials (powder from marigold inflorescences) allows to abandon artificial colorsand give the finished pasta a pleasant colorand aroma.

scholarly journals Study on domestic market of medicinal products of plant origin

2018 ◽  
pp. 30-36
Author(s):  
V. M. Minarchenko ◽  
A. Yu. Butko
Keyword(s):  
Medicinal Plants ◽  
Raw Materials ◽  
Domestic Market ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Herbal Remedies ◽  
Plant Origin ◽  
Resource Base ◽  
Natural Resource Base ◽  
Active Substances

The popularity of herbal remedies and medicines, with the participation of biologically active substances from plants is traditionally high. The annual increase of the domestic market for herbal remedies is due to the preservation and modernization of production capacities for processing of medicinal vegetative raw materials in Ukraine, introduction of modern scientific developments, a large natural resource base of medicinal plants and attracting investments in the development of this industry. The aim of our work was the study of the domestic market of medicines of plant origin, which includes herbal remedies, substances and preparations with the participation of biologically active substances from plants. The object of the study was domestic medicines of plant origin that are registered in Ukraine for 2012–2017. The study used the analytical, statistical methods, and the results of its own resource studies of medicinal plants. Phytopharmaceutical market of Ukraine is actively developing due to the attraction of new types of medicinal plants and expanding the range of medicines. The number of domestic drugs with the participation of biologically active substances from plants has increased by 17.4% compared with 2014. It is established that as of 1 January 2017 in Ukraine was 1 141 the form of medicinal drugs of plant origin. Of them: 645 – domestic products, 496 – foreign. Most of the herbal drugs in the pharmaceutical market of Ukraine are made from wild and cultivated domestic raw materials.

scholarly journals Biologically active substances from Achillea nobilis L.

2020 ◽  
Vol 100 (4) ◽  
pp. 52-59
Author(s):  
А.S. Kishkentayeva ◽  
◽  
S.N. Mantler ◽  
М.М. Zhakanov ◽  
S.М. Adekenov ◽  
...  
Keyword(s):  
Climatic Factors ◽  
Raw Materials ◽  
Sesquiterpene Lactones ◽  
Component Composition ◽  
Hot Water ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Phytochemical Study ◽  
Plant Raw Materials ◽  
Active Substances

The review summarizes data on biologically active compounds of Achillea nobilis L. and methods of their isolation. From Achillea nobilis L., collected in different places of growth, the following have been isolated: essential oil, the main components of which are monoterpene compounds; sesquiterpene lactones estafiatin, hanphyllin, anobin, chrysartemine A, canin, anolide and tanapartin-β-peroxide; the steroid acetyleucanbin; flavonoids: 3,5-dihydroxy-6,7,8-trimethoxyflavone, 5-hydroxy-3,6,7,4’-tetramethoxyflavone and 5,3’-di-hydroxy-3,6,7,4’-tetramethoxyflavone. It has been determined that the component composition of the essen-tial oil of Achillea nobilis L. largely depends on the soil and climatic factors in the places of its growth, the phase of the growing season and the method of its extraction from plant raw materials, and the extractant used (chloroform, ethanol, hot water, diethyl ether). Antibacterial, antimicrobial, antioxidant, antiparasitic activi-ties are characteristic both for the sums of extractive substances from Achillea nobilis L. and for individual compounds isolated from them. Methods for the isolation of biologically active substances from Achillea nobilis L. for the development of new drug substances are described. The main aim of this work was a com-parative analysis of the available research results on the phytochemical study of Achillea nobilis L.

BACKGROUND CONTENT OF TECHNOGENIC AND NATURAL RADIONUCLIDES IN DETOXIFYING AND BIOLOGICALLY ACTIVE SUBSTANCES

2021 ◽  
Vol 1 (2) ◽  
pp. 223-229
Author(s):  
G.А. Zhorov ◽  
◽  
L.L. Zakharova ◽  
V.N. Obryvin ◽  
◽  
...  
Keyword(s):  
Specific Activity ◽  
Raw Materials ◽  
Natural Radionuclides ◽  
Animal Husbandry ◽  
Maximum Level ◽  
Feed Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Radioactive Isotopes ◽  
Active Substances

For correctly assess the effectiveness and safety of the use of sorption-detoxifying agents and feed additives intended for animals receiving feed with an excess content of toxicants, it is necessary to take into account the background levels of radionuclides, toxic elements, pesticides and other technogenic and natural pollutants in the studied substances. The need for such studies is due both to the existence of areas with a naturally elevated level of natural toxicants in the sources of raw materials for the production of additives and drugs, and to the increasing anthropogenic influence, accompanied by the entry of xenobiotics into environmental objects. In series of radiometric studies, the specific activity of technogenic and natural radioactive isotopes (90Sr, 137Cs, 40К, 226Ra, 232Th) in a number of sorption-detoxifying and biologically active substances and preparations used in animal husbandry and veterinary medicine as part of feed additives and pharmacological agents was determined. It was found that in 43% of the studied samples, the level of specific activity of 1,1Sr in 3-90 times higher than allowed by the current standards. The maximum level of 90Sr, equal to (137±9) Bq/kg, was detected in perlite. The specific activity of 137Cs did not exceed the permissible level: in mineral sorbents its amount reached (40±7) Bq/kg, in organic and complex sorbents – (24±4), and in feed additives – (29±8) Bq/kg. The maximum levels of 40K were (1429±83) Bq/ kg in minerals (radionite) and (2613±100) Bq/kg in organic substances (lignohumate). The levels of 226Ra and 232Th did not exceed (153±13) and (79±13) Bq/kg, respectively, and were higher in the mineral samples.

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