scholarly journals Association of rs266729 and rs16861194 polymorphisms of the ADIPOQ gene with the risk of obesity in residents of the Moscow region

2020 ◽  
Vol 49 ◽  
Author(s):  
A. V. Pogozheva ◽  
E. Yu. Sorokina
Keyword(s):  
Real Time ◽  
European Population ◽  
Overweight And Obesity ◽  
Minor Allele ◽  
Moscow Region ◽  
Real Time System ◽  
Adipoq Gene ◽  
Glucose Levels ◽  
Allele Specific ◽  
Specific Amplification

Rationale: The contribution of the adiponectin gene polymorphisms (ADIPOQ, located at 3q27) in the residents of Nigeria and China to the risk of overweight and its association with the risk of arterial hypertension in the European population have been demonstrated.Aim: To identify associations between rs266729 and rs16861194 polymorphisms of the ADIPOQ gene with overweight and obesity in the Moscow region residents.Materials and methods: Identification of rs266729 and rs16861194 polymorphisms of the ADIPOQ gene was carried out in 222 people (140 women and 82 men, aged 25 to 65 years) living in the Moscow region. Genotyping was performed using allele-specific amplification with real-time detection of the results on the CFX96 Real-Time System amplifier (Bio-Rad, USA) and using TaqMan probes complementary to polymorphic DNA regions. To assess an association between these genetic polymorphisms and overweight/obesity, we performed a case control study, with the cases being subjects with body mass index (BMI) of ≥ 30 kg/m2, and the controls those with BMI of < 30 kg/m2.Results: The mean frequency of the minor allele G rs266729 polymorphism of the ADIPOQ gene in the subjects from the Moscow region was 26.8% and similar in men and women. Comparison of the CC and GG genotypes carriers of the rs266729 polymorphism of the ADIPOQ gene in men showed a statistically significant association of the GG genotype to the BMI value (p = 0.04). There were no statistically significant differences between anthropometric indicators (BMI, body fat mass) in the carriers of different rs16861194 polymorphism genotypes of the ADIPOQ. No association between the studied polymorphisms and blood glucose levels and lipid spectrum could be found.Conclusion: The frequency of the minor allele G of the rs266729 polymorphism of the ADIPOQ gene in the subjects from the Moscow region was similar to their rates in the Russian Federation and European countries. In Moscow residents, the rs266729 polymorphism of the ADIPOQ gene (G allele) contributes to the risk of obesity in homozygous carriers (genotype GG). No association of the rs16861194 polymorphism of the ADIPOQ gene with the BMI was found; therefore, this polymorphism cannot be considered as a genetic marker of the obesity risk.

scholarly journals Sky View Factor (SVF) assessment in a real-time system for thermal comfort conditions monitoring in the Moscow region

2020 ◽  
Vol 611 ◽  
pp. 012061
Author(s):  
A A Perkhurova ◽  
M I Varentsov ◽  
T E Samsonov ◽  
P E Kargashin ◽  
P A Korosteleva ◽  
...  
Keyword(s):  
Thermal Comfort ◽  
Real Time ◽  
Moscow Region ◽  
View Factor ◽  
Time System ◽  
Real Time System ◽  
Sky View Factor

Real-time allele-specific amplification for sensitive detection of the BRAF mutation V600E

2004 ◽  
Vol 18 (5) ◽  
pp. 349-352 ◽  
Author(s):  
Anne Jarry ◽  
Damien Masson ◽  
Elisabeth Cassagnau ◽  
Sigrid Parois ◽  
Christian Laboisse ◽  
...  
Keyword(s):  
Real Time ◽  
Braf Mutation ◽  
Sensitive Detection ◽  
Allele Specific ◽  
Specific Amplification

scholarly journals Evaluation of an association of the rs1801133 MTHFR gene polymorphism with folic acid deficiency in obese patients

2018 ◽  
Vol 46 (3) ◽  
pp. 254-257 ◽  
Author(s):  
A. V. Pogozheva ◽  
E. Yu. Sorokina ◽  
T. V. Aristarkhova
Keyword(s):  
Folic Acid ◽  
Gene Polymorphism ◽  
Mthfr Gene ◽  
Overweight And Obesity ◽  
Moscow Region ◽  
Folic Acid Deficiency ◽  
Obese People ◽  
Real Time System ◽  
Mthfr Gene Polymorphism ◽  
Acid Deficiency

Background: The use of molecular genetic technologies has made it possible to show that the genetic factor plays a significant role in the development of obesity. In addition, in obese people the supply with vitamins, in particular with folic acid, is largely controlled genetically.Aim: To study an association of the rs1801133 polymorphism of the MTHFR gene with folic acid deficiency in the residents of the Moscow region depending on their body mass index.Materials and methods: rs1801133 polymorphisms were identified in 326 subjects (74 male and 252 female) aged from 20 to 65 years, living in the Moscow region. The DNA was isolated from blood by the sorption on silica gel-coated magnetic particles. DNA was isolated with the use of the epMotion 5075 automatic station (Eppendorf, Germany). To identify the polymorphism, a polymerase chain reaction was used, followed by cleavage of the Hinf1 restriction endonuclease products, with analysis of these products by gel electrophoresis. The equipment CFX96 Real Time System (BIO-RAD, USA) was used. Folic acid was measured by ID-Vit® Folic Acid test system (R-Biopharm, Germany).Results: According to the results of folic acid measurements in blood, a deficiency of this vitamin was found in 24.2% of the studied residents of the Moscow region. Analysis of the genotyping results did not show any association of the rs1801133 MTHFR gene polymorphism with the serum levels of folic acid. However, in the subjects with overweight and obesity, there was a statistically significant association between the T allele of the rs1801133 of the MTHFR gene polymorphism and a low level of folic acid (odds ratio 2.5, 95% confidence interval 1.09–5.74, p = 0.03).Conclusion: The rs1801133 polymorphism of the MTHFR gene significantly contributes to the development of folic acid deficiency in overweight and obese individuals.

scholarly journals Rapid, Long-Range Molecular Haplotyping of Thiopurine S-Methyltransferase (TPMT*) *3A, *3B, and *3C

2004 ◽  
Vol 50 (9) ◽  
pp. 1528-1534 ◽  
Author(s):  
Nicolas von Ahsen ◽  
Victor W Armstrong ◽  
Michael Oellerich
Keyword(s):  
Real Time ◽  
Long Range ◽  
High Throughput Screening ◽  
Genomic Region ◽  
Nucleotide Polymorphisms ◽  
Hybridization Probes ◽  
Pcr Product ◽  
Allele Specific ◽  
Specific Amplification ◽  
Long Range Pcr

Abstract Background: Haplotyping is an important technique in molecular diagnostics because haplotypes are often more predictive for individual phenotypes than are the underlying single-nucleotide polymorphisms (SNPs). Until recently, methods for haplotyping SNPs separated by kilobase distances were laborious and not applicable to high-throughput screening. In the case of thiopurine S-methyltransferase (TPMT*), differentiating among TPMT*3A, *3B, and *3C alleles is sometimes necessary for predictive genotyping. Methods: The genomic region including the two SNPs that define TPMT*3A, *3B, and *3C alleles was amplified by long-range PCR. The resulting PCR product was circularized by ligation and haplotyped by allele-specific amplification PCR followed by product identification with hybridization probes. Results: Critical points were the long-range PCR conditions, including choice of buffer and primers, optimization of the ligation reaction, and selection of primers that allowed for strict allele-specific amplification in the second-round PCR. Different underlying TPMT haplotypes could then be differentiated. Results from the haplotyping method were in full agreement with those from our standard real-time PCR method: TPMT*1/*3A (n = 20); TPMT*1/*3C (n = 4); TPMT*1/*1 (n = 6); and TPMT*3A/*3A (n = 6). One TPMT*1/*3A sample failed to amplify, and no whole blood was available for repeat DNA isolation. Conclusions: This method for rapid-cycle real-time, allele-specific amplification PCR-assisted long-range haplotyping has general application for the haplotyping of distant SNPs. The procedure is simpler and more rapid than previous methods. With respect to TPMT, haplotyping has the potential to discriminate the genotypes TPMT*1/*3A (intermediate metabolizer) and TPMT*3B/*3C (poor metabolizer).

Breakfast Skipping Is an Independent Predictor of Obesity but not Overweight Among Children in a Southeastern European Population

2016 ◽  
Vol 86 (5-6) ◽  
pp. 242-248 ◽  
Author(s):  
Genc Burazeri ◽  
Jolanda Hyska ◽  
Iris Mone ◽  
Enver Roshi
Keyword(s):  
Task Force ◽  
Demographic Data ◽  
Cross Sectional Study ◽  
European Population ◽  
Overweight And Obesity ◽  
World Health ◽  
Communist Country ◽  
Western Balkans ◽  
Cross Sectional ◽  
Breakfast Skipping

Abstract.Aim: To assess the association of breakfast skipping with overweight and obesity among children in Albania, a post-communist country in the Western Balkans, which is undergoing a long and difficult political and socioeconomic transition towards a market-oriented economy. Methods: A nationwide cross-sectional study was carried out in Albania in 2013 including a representative sample of 5810 children aged 7.0 – 9.9 years (49.5% girls aged 8.4 ± 0.6 years and 51.5% boys aged 8.5 ± 0.6 years; overall response rate: 97%). Children were measured for height and weight, and body mass index (BMI) calculated. Cut-off BMI values of the World Health Organization (WHO) and the International Obesity Task Force (IOTF) were used to define overweight and obesity in children. Demographic data were also collected. Results: Upon adjustment for age, sex, and place of residence, breakfast skipping was positively related to obesity (WHO criteria: OR = 1.5, 95% CI = 1.3–1.9; IOTF criteria: OR = 1.9, 95% CI = 1.4–2.5), but not overweight (OR = 1.1, 95% CI = 0.9–1.3 and OR = 1.1, 95% CI = 0.9–1.4, respectively). Furthermore, breakfast skipping was associated with a higher BMI (multivariable-adjusted OR = 1.05, 95% CI = 1.02–1.07). Conclusions: Our findings point to a strong and consistent positive relationship between breakfast skipping and obesity, but not overweight, among children in this transitional southeastern European population. Future studies in Albania and other transitional settings should prospectively examine the causal role of breakfast skipping in the development of overweight and obesity.

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

Real-Time System for Sending Data from Three Clients to One Server with MATLAB

2015 ◽  
Vol 2 (1) ◽  
pp. 35-41
Author(s):  
Rivan Risdaryanto ◽  
Houtman P. Siregar ◽  
Dedy Loebis
Keyword(s):  
Information System ◽  
Real Time ◽  
Time System ◽  
Real Time System ◽  
The Real ◽  
Performance Time ◽  
Data Process ◽  
Effectiveness And Efficiency

The real-time system is now used on many fields, such as telecommunication, military, information system, evenmedical to get information quickly, on time and accurate. Needless to say, a real-time system will always considerthe performance time. In our application, we define the time target/deadline, so that the system should execute thewhole tasks under predefined deadline. However, if the system failed to finish the tasks, it will lead to fatal failure.In other words, if the system cannot be executed on time, it will affect the subsequent tasks. In this paper, wepropose a real-time system for sending data to find effectiveness and efficiency. Sending data process will beconstructed in MATLAB and sending data process has a time target as when data will send.

The Goal-Setting Model in a Multiagent Hard Real-Time System

Vestnik MEI ◽  
2018 ◽  
Vol 5 (5) ◽  
pp. 73-78
Author(s):  
Igor В. Fominykh ◽  
◽  
Sergey V. Romanchuk ◽  
Nikolay Р. Alekseev ◽  
◽  
...  
Keyword(s):  
Real Time ◽  
Goal Setting ◽  
Time System ◽  
Real Time System ◽  
Hard Real Time
Sign in / Sign up

Export Citation Format

Share Document