Study of Combining Ability and Heterosis in Quality Protein Maize using Line x Tester Mating Design

Background: High protein content with good quality maize is likely to gain wider acceptance if hybrids are produced that have agronomic performance similar to normal hybrids and retain an enhanced nutritional quality. Thus, an attempt was made to develop quality protein maize hybrids for the benefit of different sections of society depending on maize. Methods: During the Rabi season of 2016-17 (hybrids were obtained crossing ten inbred lines and four testers following Line x Tester mating design) and 2017-18 (evaluation of hybrids and parents involved along with two commercial checks based on different agro-morphological traits). Result: Highly significant differences were observed among lines, testers and crosses. Combining ability revealed SCA variances was higher indicating preponderance of non-additive gene action. The contribution of line x tester interaction was higher suggesting parents used in this study can provide high heterotic cross combinations. The parents CML 508, CML 163-D and CML 169 were superior general combiners, indicating their efficacy as parents in future maize breeding programs. Heterosis identified superior yield advantage of the crosses namely, CML508 x CML154-2, CML170 x CML169 and CML163-D x DMRQPM103 over the standard commercial checks HQPM1 and 900 M Gold.

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An Evaluation of Kernel Zinc in Hybrids of Elite Quality Protein Maize (QPM) and Non-QPM Inbred Lines Adapted to the Tropics Based on a Mating Design

Agronomy ◽

10.3390/agronomy10050695 ◽

2020 ◽

Vol 10 (5) ◽

pp. 695 ◽

Cited By ~ 1

Author(s):

Edna K. Mageto ◽

Michael Lee ◽

Thanda Dhliwayo ◽

Natalia Palacios-Rojas ◽

Félix San Vicente ◽

...

Keyword(s):

Combining Ability ◽

Gene Action ◽

Inbred Lines ◽

Quality Protein Maize ◽

Zn Deficiency ◽

Mean Values ◽

Mating Design ◽

Zn Concentration ◽

Additive Gene ◽

The Tropics

Genetic improvement of maize with elevated levels of zinc (Zn) can reduce Zn deficiency among populations who rely on maize as a staple. Inbred lines of quality protein maize (QPM) and non-QPM with elevated Zn levels in the kernel have been identified. However, information about the optimal strategy to utilize the germplasm in breeding for high-Zn concentration is lacking. As a preliminary step, this study was conducted to ascertain the potential of QPM, non-QPM, or a combination of QPM and non-QPM hybrids for attaining desirable Zn concentration. Twenty elite inbreds, 10 QPM and 10 non-QPM, were crossed according to a modified mating design to generate hybrids, which were evaluated in four environments in Mexico during 2015 and 2016 in order to evaluate their merits as parents of hybrids. The highest mean values of Zn were observed when high-Zn QPM lines were crossed with high-Zn non-QPM lines. Hybrids with high Zn and grain yield were identified. General combining ability (GCA) effects for Zn concentration were more preponderant than specific combining ability (SCA) effects, suggesting the importance of additive gene action for the inheritance of Zn.

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The Effects of Combining Ability for Yield Traits in Rice (Oryza sativa L.) under Sodic Soil

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2020/v39i1230661 ◽

2020 ◽

pp. 34-44

Author(s):

Durgesh Kumar Shukla ◽

S. N. Singh ◽

S. C. Gaur ◽

Anil Kumar

Keyword(s):

Combining Ability ◽

Gene Action ◽

Oryza Sativa L ◽

Hybrid Vigour ◽

Yield Traits ◽

Additive Gene Action ◽

Early Maturity ◽

Additive Gene ◽

Rice Genotypes ◽

Positive Effect

Information on combining ability is derived from data on twelve yield and yield contributing characters in fifteen male and three female parents utilised in line x tester fashion to estimate combining ability of rice genotypes under coastal saline condition. Forty Five hybrids generated from crossing three lines with fifteen testers were studied along with their parents for combining ability and gene action involved in the expression of characters in rice. The gca and sca effects were significant for all the characters. The magnitude of sca variance was higher than the gca variance for all the characters revealed the presence of predominance of non-additive gene action for all the characters under study. Halchal (-12.29) was found to be good general combiners for days to 50% flowering and early maturity, Halchal (-13.39). However, IR-24 (1.88) was good general combiners with significant positive effect for tallness. Magic (-12.05) good general combiners for dwarfness and Shriram 434 (1.57) was good general combiners for test weight however, Moti was best general combiner with gca estimates of 7.07 for harvest index and Kuber (3.48) was the good general combiners for yield/hill. Cross combinations RHR 27 x IR 24 (4.04) was significant and positive effect for yield/hill, performance for ear bearing tillers per plant were RHR 27 x IR 24 (1.88). In general, the crosses showing significant and desirable combining ability effects were associated with better per se performance for the respective traits. These hybrids could be utilized in heterosis breeding to exploit hybrid vigour.

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Assessment of Combining Ability Effects for several Yield and Quality Traits in a Complete Diallel Cross of Strawberry (Fragaria × ananassa Duch.)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha45210873 ◽

2017 ◽

Vol 45 (2) ◽

pp. 517-524

Author(s):

Sorin CIULCA ◽

Natalia CARP ◽

Emilian MADOŞA ◽

Adriana CIULCA ◽

Radu ŞUMĂLAN

Keyword(s):

Combining Ability ◽

Gene Action ◽

Sugar Content ◽

Fruit Weight ◽

Yield Traits ◽

Quality Traits ◽

Additive Gene Action ◽

Plant Yield ◽

Yield And Quality ◽

Additive Gene

Information on the inheritance of yield and quality traits is important for the selection of parents and breeding approaches to be adopted for the improvement of strawberry. The present study aimed to estimate the combining ability and gene effects for plant yield, fruits number/plant, fruit weight, pulp firmness and sugar content of strawberries in order to identify the best genitors and promising crosses, in 30 hybrids of six parents. The additive and non-additive gene action as well as the maternal effects was involved in the inheritance of the studied traits. For all traits, especially for fruit weight and plant yield, the additive gene action was more important than the non-additive one. The parents ‘A1’ and ‘Alba’ showed a higher concentration of favourable alleles for plant yield and fruits number, and they will allow the increase of plant yield when used as a donor of pollen and the increase of fruit number when used as a recipient of pollen. The variety ‘Marmolada’ was a good general combiner for sugar content, pulp firmness and fruit weight, especially when used as a female genitor. These parents could be used in hybridization in order to accelerate the genetic improvement of some yield and quality traits in strawberries. The cross ‘Mira’ × ‘Onda’ expressed desirable specific combining ability effects for yield traits and can be successfully use in strawberries breeding programs. In the case of ‘Alba’ × ‘Clery’ there is a high probability to select progenies with valuable yield traits associated with sweet fruits.

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Combining Ability Analysis of Blast Disease Resistance and Agronomic Traits in Finger Millet [Eleusine coracana (L.) Gaertn]

Journal of Agricultural Science ◽

10.5539/jas.v8n11p138 ◽

2016 ◽

Vol 8 (11) ◽

pp. 138 ◽

Cited By ~ 1

Author(s):

Lawrence Owere ◽

Pangirayi Tongoona ◽

John Derera ◽

Nelson Wanyera

Keyword(s):

Disease Resistance ◽

Combining Ability ◽

Finger Millet ◽

Gene Action ◽

Agronomic Traits ◽

The Other ◽

Blast Disease ◽

Additive Gene Action ◽

Gene Effects ◽

Additive Gene

Blast disease is the most important biotic constraint to finger millet production. Therefore disease resistant varieties are required. However, there is limited information on combining ability for resistance and indeed other agronomic traits of the germplasm in Uganda. This study was carried out to estimate the combining ability and gene effects controlling blast disease resistance and selected agronomic traits in finger millet. Thirty six crosses were generated from a 9 × 9 half diallel mating design. The seed from the 36 F1 crosses were advanced by selfing and the F2 families and their parents were evaluated in three replications. General combining ability (GCA) for head blast resistance and the other agronomic traits were all highly significant (p ≤ 0.01), whereas specific combining ability (SCA) was highly significant for all traits except grain yield and grain mass head-1. On partitioning the mean sum of squares, the GCA values ranged from 31.65% to 53.05% for head blast incidence and severity respectively, and 36.18% to 77.22% for the other agronomic traits measured. Additive gene effects were found to be predominant for head blast severity, days to 50% flowering, grain yield, number of productive tillers plant-1, grain mass head-1, plant height and panicle length. Non-additive gene action was predominant for number of fingers head-1, finger width and panicle width. The parents which contributed towards high yield were Seremi 2, Achaki, Otunduru, Bulo and Amumwari. Generally, highly significant additive gene action implied that progress would be made through selection whereas non-additive gene action could slow selection progress and indicated selection in the later generations.

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Combining ability and gene action studies for yield-contributing traits in crosses involving winter and spring wheat genotypes

Acta Agronomica Hungarica ◽

10.1556/aagr.57.2009.4.4 ◽

2009 ◽

Vol 57 (4) ◽

pp. 417-423 ◽

Cited By ~ 1

Author(s):

S. Sharma ◽

H. Chaudhary

Keyword(s):

Winter Wheat ◽

Spring Wheat ◽

Combining Ability ◽

Gene Action ◽

Block Design ◽

Additive Gene Action ◽

Wheat Genotypes ◽

Diallel Mating Design ◽

Additive Gene ◽

Wheat Hybrids

The success of winter × spring wheat hybridization programmes depends upon the ability of the genotypes of these two physiologically distinct ecotypes to combine well with each other. Hence the present investigation was undertaken to study the combining ability and nature of gene action for various morpho-physiological and yield-contributing traits in crosses involving winter and spring wheat genotypes. Five elite and diverse genotypes each of winter and spring wheat ecotypes and their F 1 (spring × spring, winter × winter and winter × spring) hybrids, generated in a diallel mating design excluding reciprocals, were evaluated in a random block design with three replications. Considerable variability was observed among the spring and winter wheat genotypes for all the traits under study. Furthermore, these traits were highly influenced by the winter and spring wheat genetic backgrounds, resulting in significant differences between the spring × spring, winter × winter and winter × spring wheat hybrids for some of the traits. The winter × spring wheat hybrids were observed to be the best with respect to yieldcontributing traits. On the basis of GCA effects, the spring wheat parents HPW 42, HPW 89, HW 3024, PW 552 and UP 2418 and the winter wheat parents Saptdhara, VWFW 452, W 10 and WW 24 were found to be good combiners for the majority of traits. These spring and winter wheat parents could be effectively utilized in future hybridization programmes for wheat improvement. Superior hybrid combinations for one or more traits were identified, all of which involved at least one good general combiner for one or more traits in their parentage, and can thus be exploited in successive generations to develop potential recombinants through various breeding strategies. Genetic studies revealed the preponderance of additive gene action for days to flowering, days to maturity and harvest index, and non-additive gene action for the remaining six traits.

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Studies on combining ability in high yielding drought tolerant mungbean genotypes under West Bengal condition

Legume Research - An International Journal ◽

10.18805/lr-3537 ◽

2018 ◽

Cited By ~ 1

Author(s):

M Sen ◽

D K De

Keyword(s):

Combining Ability ◽

West Bengal ◽

Gene Action ◽

The Other ◽

Additive Gene Action ◽

Drought Tolerant ◽

Combining Ability Analysis ◽

Additive Gene ◽

Seedling Characters ◽

Half Diallel

Combining ability analysis was carried out in an 8x8 half-diallel fashion in mungbean to understand the combining ability, nature of gene action for thirteen yield and its components in 28 hybrids and their 8 parents. These 8 genotypes were already classified into drought tolerant and drought susceptible types from a laboratory study where PEG (6000) (-3) bar was used to impose drought stress against control for studying the seedling characters. The analysis of variance due to combining ability for the thirteen yield attributing traits in F1 population and their parents revealed that variances due to GCA and SCA were highly significant for all the characters indicating that these traits were controlled by both additive and non-additive gene action. Results also showed that cross combinations producing significantly superior SCA effect generally involved one of the parents with good GCA effect and the other had been either medium or poor combiner. Transgressive breeding has been opined to be useful in such cases. After compilation of the results it was found that only two cross combinations viz. SML-286(S) x B-1(T) and PDM-54(T) x K-851(T) were superior performers with respect to 7 and 5 characters including yield. Therefore, progeny of these two crosses may be pursued for obtaining lines with higher yield and tolerance to drought.

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HERITABILITY FOR COMBINING ABILITY OF SEEDLING VIGOR IN BIRDSFOOT TREFOIL

Canadian Journal of Plant Science ◽

10.4141/cjps72-179 ◽

1972 ◽

Vol 52 (6) ◽

pp. 1059-1065 ◽

Cited By ~ 3

Author(s):

B. E. TWAMLEY

Keyword(s):

Seed Size ◽

Combining Ability ◽

Gene Action ◽

Birdsfoot Trefoil ◽

Seedling Vigor ◽

Additive Gene Action ◽

Predictive Values ◽

Additive Gene ◽

Estimate Heritability ◽

Selection Of

Twenty birdsfoot trefoil families were studied, first, to investigate variation in seed size and seedling vigor; second, to determine predictive values of parental performance; and third, to estimate heritability for combining ability. The experimental material consisted of open-pollination seed collected from 20 parents and 10 daughters of each. It was examined for seed size and was used for a seedling vigor test. Wide variation for both seed size and for seedling vigor existed both within and among families. The performance of the parents with respect to neither seed size nor seedling vigor was a valid indicator of the combining ability of the daughters. Heritability for these traits was low. In the discussion, the postulate was made that in selecting material for the Syn 0 generation of a new cultivar on the basis of combining ability, greater emphasis should be placed on additive gene action. The program would involve the selection of only those plants that, in addition to being high in combining ability themselves, had offspring with a high combining ability average.

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Combining ability analysis in near homozygous lines of okra [Abelmoschus esculentus (L.) Moench] for yield and yield attributing parameters

Journal of Applied and Natural Science ◽

10.31018/jans.v9i1.1191 ◽

2017 ◽

Vol 9 (1) ◽

pp. 324-331

Author(s):

Y. A. Lyngdoh ◽

R. Mulge ◽

A. Shadap ◽

Jogendra Singh ◽

Seema Sangwan

Keyword(s):

Combining Ability ◽

Genetic Variance ◽

Gene Action ◽

Additive Genetic Variance ◽

Total Yield ◽

Fruit Weight ◽

Additive Gene Action ◽

Combining Ability Analysis ◽

Additive Gene ◽

Number Of Branches

Line × tester analysis was carried out with the objective of identifying the good combiners and to decide the breeding strategies for developing potential and productive genotypes or cultivars. Parents and hybrids differed significantly for GCA and SCA effects for all the characters respectively. Specific combining ability (SCA) variance was higher than the general combining ability (GCA) variance which shows the predominance of non-additive gene action for the improvement of all the characters studied. The parents and crosses having highest and significant GCA and SCA effects viz., KO-18 (13.69), KO-6 (9.54) and KO-2 × Parbhani Kranti (19.28) for plant height; KO-12 (0.34), KO-14 (0.19) and KO-5 × V5 (0.60) for number of branches per plant; KO-14 (-0.66) and KO-15 × Arka Anamika(-1.66) for days to first flowering; KO-1(1.10), Arka Anamika (0.46) and KO-9 × VRO-5 (3.28) for fruit length; KO-7 (7.91), VRO-5(1.68) and KO-18 × VRO-6 (8.64) for average fruit weight; KO-2 (1.18) and KO-17 × Arka Anamika (2.80) for number of fruits per plant; KO-9(0.05), VRO-6 (0.01) and KO-11 × VRO-6 (0.10) for total yield per plant were identified as good general and specific combiners. The results establish the worth of heterosis breeding for effective usage of non-additive genetic variance in okra.

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The combining ability of recombinant S-lines developed from an F2 maize population

The Journal of Agricultural Science ◽

10.1017/s0021859699007406 ◽

2000 ◽

Vol 134 (2) ◽

pp. 191-198 ◽

Cited By ~ 5

Author(s):

C. G. IPSILANDIS ◽

M. KOUTSIKA-SOTIRIOU

Keyword(s):

Combining Ability ◽

Gene Action ◽

Inbred Lines ◽

High Yield ◽

Source Population ◽

Additive Gene Action ◽

Maize Population ◽

Ability Tests ◽

Additive Gene ◽

Per Se

Starting with the F2 generation of the single-cross commercial hybrid Lorena (PR3183), recombinant lines were developed combining half-sib/S1 evaluation on widely spaced plants in the direction of high yielding per se. Combining ability tests consisted of crosses between: (a) recombinant lines of common pedigree and (b) recombinant lines and freely available inbred lines. The highest-yielding crosses between recombinant lines reached 100% of the original F1 hybrid in a percentage of 14·2. Low heterosis was estimated owing to additive gene action of recombinant lines. Crosses between recombinant lines and freely available inbred lines outyielded significantly the commercial F1 hybrid in a percentage of 33·3. Heterosis was greater and the original F1 hybrid was outyielded significantly because of non-additive gene action. When the applied breeding procedure on a source population with high yield adaptability is adopted and where effects of intergenotypic competition masking the inherent genotypic value are controlled, population improvement may be substituted by combined half-sib/S1 selection for productivity of lines per se in low stress conditions during the very early stages.

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Combining Ability And Heterosis In Snakegourd (Tricosanthes Cucurminata L)

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v23i2.9318 ◽

1970 ◽

Vol 23 (2) ◽

pp. 1-6

Author(s):

R Podder ◽

MG Rasul ◽

AKMA Islam ◽

Mak Mian ◽

JU Ahmed

Keyword(s):

Combining Ability ◽

Gene Action ◽

Female Flower ◽

Fruit Yield ◽

High Yield ◽

Additive Gene Action ◽

Combining Ability Analysis ◽

Additive Gene ◽

Half Diallel ◽

Fruit Diameter

A half diallel set of five parents and their 10 F1’s were studied to determine the combining ability and magnitude of heterosis for eight important characters in snakegourd at the experimental field of Bangabandhu Sheikh Mujibur Rahman Agricultural University during April to July, 2004. Combining ability analysis revealed that both general and specific combining ability variances were significant for all the characters except fruit diameter and fruit yield per plant. Predominance of additive gene action was noted for all the characters except days to first female flower where non-additive gene action was predominant. Parent P1 was the best general combiner for fruit yield and some yield contributing characters. Among the crosses P2 X P3, P1 X P2 and P1 X P4 were the best specific combiner for fruit yield and some of yield contributing characters. Both positive and negative heterosis was obtained of which few hybrids showed desirable and significant values. P2 X P5 showed the highest significant mid parental heterotic value for earliness and high yield whereas, P1 X P2, P2 X P3, P2 X P5 and P3 X P4 showed the highest significant better parent heterotic effect for earliness and high yield. Key words: Snakegourd (Trichosanthes cucurminata L.); combining ability; heterosis; fruit yieldDOI: http://dx.doi.org/10.3329/bjpbg.v23i2.9318 Bangladesh J. Pl. Breed. Genet., 23(2): 1-6, 2010

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