Effect of imidacloprid on antioxidant status and histopathological changes in ovary and uterus of adult female wistar rats

2018 ◽  
Author(s):  
Archana Lohiya ◽  
Vinod Kumar ◽  
J.S. Punia
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Wistar Rats ◽  
Control Programme ◽  
Female Rats ◽  
Histopathological Changes ◽  
Gum Acacia ◽  
Flea Control ◽  
Female Wistar Rats ◽  
Neonicotinoid Insecticide

Imidacloprid is a neonicotinoid insecticide and has been extensively used as a crop pest and in pet flea control programme. In the present study, the effects of imidacloprid on ovary and uteri tissue was analyzed in adult female Wistar rats at two dose levels (19 and 38 mg/kg/day) administered orally for 10, 20 and 30 days. Effects were compared with respective control animals administered daily with 2% gum acacia. Parameters undertaken were organ weight, levels of cytoplasmic and membrane proteins, oxidative stress parameters viz. activities of SOD, GPx and levels of GSH and MDA and histopathological changes. IMI (38 mg/kg, 30 days) reduced cytoplamic proteins in both ovaries and uteri whereas this dose reduced membrane protein in ovaries only. IMI (38 mg/kg, 20 and 30 days) decreased SOD enzyme in both ovaries and uteri and GSH-Px levels in ovaries only. The GSH-Px levels were also seen with decreased levels in uteri with IMI (38 mg/kg) for 30 days. IMI (38 mg/kg, 20 and 30 days) induces degenerative changes in ovaries of rats. Hence, it is concluded from the present studies that administration of higher doses (38 mg/kg/day) of IMI for 20 and 30 days generated oxidative stress in ovaries and uteri of female rats.

Sub-acute oxidant and histopathological effects of imidacloprid on kidney of adult female Wistar rats

2018 ◽  
Author(s):  
Archana Lohiya ◽  
Vinod Kumar ◽  
J. S. Punia
Keyword(s):  
Oxidative Stress ◽  
Membrane Proteins ◽  
Adult Female ◽  
Wistar Rats ◽  
Kidney Tissue ◽  
Control Programme ◽  
Histopathological Changes ◽  
Flea Control ◽  
Female Wistar Rats ◽  
Neonicotinoid Insecticide

Imidacloprid is a neonicotinoid insecticide and has been extensively used as a crop pest and pet flea control programme. In the present study, the effects of imidacloprid on kidney tissue were analyzed in adult female Wistar rats at two dose levels (19 and 38 mg/kg/day) administered orally for 10, 20 and 30 days. Effects were compared with respective control animals administered daily with 2% gum acacia. Different parameters undertaken were kidney weight, levels of cytoplasmic and membrane proteins, oxidative stress parameters viz. activities of SOD, GPx and levels of GSH and MDA and histopathological changes. Imidacloprid at 38 mg/kg dose administered orally for 20 and 30 days significantly increased organ weight and levels of MDA. The activities of enzymes SOD and GPx and levels of GSH were decreased significantly at 38 mg/kg dose administered daily for 30 days. There was significant decrease observed in levels of cytoplasmic and membrane proteins at 38 mg/kg dose administered orally for 20 and 30 days. Histopathological changes in kidney at 38 mg/kg dose in 20 and 30 days group. Therefore, it is concluded that imidacloprid has generated oxidative stress in kidney at 38 mg/kg dose administered orally for 20 and 30 days in adult female Wistar rats.

scholarly journals Effects of long-term construction noise on health of adult female Wistar rats

2017 ◽  
Vol 20 (1) ◽  
pp. 155-165 ◽  
Author(s):  
J. Zymantiene ◽  
R. Zelvyte ◽  
I. Pampariene ◽  
A. Aniuliene ◽  
N. Juodziukyniene ◽  
...  
Keyword(s):  
Adult Female ◽  
Wistar Rats ◽  
Female Rats ◽  
Histopathological Changes ◽  
Group I ◽  
Study Results ◽  
Female Wistar Rats ◽  
Group Ii ◽  
Caecal Microbiota

Abstract The aim of this study was to investigate the influence of long-term building construction noise from refurbishment, which including vibration, on some physiological parameters and histopathological changes of organs of Wistar rats. Twenty 12 month old female rats were divided into two groups: rats group I (n = 10) were exposed to long-term construction noise and rats group II (n = 10) were kept under normal noise level. Study results revealed that long-term construction noise from building refurbishment has an influence on body weight, haematological and some serum biochemical parameters affects caecal microbiota, and causes histopathological changes in the organs of adult female Wistar rats. It was noticed that rats in group I exihibited significantly higher mean values for total protein, albumin and lower values for glucose, AST, ALT, blood urea nitrogen, haematological and caecal microbiota parameters than rats in group II. The most common pathologies were determined in the kidney, liver and lungs. Other observed pathologies were lymphadenopathy, catarrhal inflammation of the intestines, spleen hyperplasia and mammary gland adenofibroma. Single cases were subcutaneous fibroma in the thoracic region, abortus with uterine inflammation and thymus hyperplasia with formation of cysts were found.

Cyantraniliprole impairs reproductive parameters by inducing oxidative stress in adult female wistar rats

2021 ◽  
Author(s):  
Suellen Ribeiro da Silva Scarton ◽  
Felipe Tsuzuki ◽  
Marina Trevizan Guerra ◽  
Dayane Priscila dos Santos ◽  
Aldair Casagrande dos Santos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Wistar Rats ◽  
Reproductive Parameters ◽  
Female Wistar Rats

Effects of chia (Salvia hispanica L.) on oxidative stress and inflammation in ovariectomized adult female Wistar rats

2019 ◽  
Vol 10 (7) ◽  
pp. 4036-4045 ◽  
Author(s):  
Bárbara Pereira da Silva ◽  
Renata Celi Lopes Toledo ◽  
Marcella Duarte Villas Mishima ◽  
Maria Eliza de Castro Moreira ◽  
Christiane Mileib Vasconcelos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Lipid Profiles ◽  
Ovariectomized Rats ◽  
High Fat ◽  
Salvia Hispanica ◽  
Female Wistar Rats ◽  
Salvia Hispanica L

The study investigated the influence of chia consumption on inflammation, oxidative stress, and lipid profiles in female ovariectomized rats fed a high-fat diet.

scholarly journals Histological and hormonal studies of calabash chalk on ovarian function in adult female wistar rats

2017 ◽  
Vol 34 (03) ◽  
pp. 173-177
Author(s):  
A. Oyewopo ◽  
K. Obasi ◽  
K. Anumudu ◽  
E. Yawson
Keyword(s):  
Body Weight ◽  
Adult Female ◽  
Wistar Rats ◽  
Ovarian Function ◽  
Female Rats ◽  
Control Group ◽  
Treatment Groups ◽  
Female Wistar Rats ◽  
Group A ◽  
Group B

Abstract Introduction: Calabash chalk is a naturally occurring mineral, chiefly composed of fossilized sea shells. It is prepared from clay and mud mixed with other ingredients, including lead, arsenic, sand and wood ash. Clay consumption is correlated with pregnancy, and also to eliminate morning sickness in women. The aim of this study is to evaluate the effects of calabash chalk on the ovarian function in adult female Wistar rats. Methods: Eighteen (18) adult female Wistar rats. Group A served as the control group, group B received 40 mg/kg body weight of Non-salted calabash chalk while group C received 40 mg/kg body weight of Salted calabash chalk for 14 days. On day 15, the animals were sacrificed for histological and biochemical examination. Results: Results showed a significant (P< 0.05) reduction in follicle stimulating hormone (FSH) levels of the treatment groups when compared with the control group and an increase in luteinizing hormone (LH) levels of the treatment groups when compared with the control group. Histological examination of the ovaries showed severe deterioration of the ovarian follicles, necrosis and follicular atresia. Conclusion: Calabash chalk is toxic to ovarian function. These alterations have been shown to be the leading cause ofinfertility in female rats. Hence, proper monitoring, education, and regulation of the product is needed.

Acupuncture and moxibustion stimulate fibroblast proliferation and neoangiogenesis during tissue repair of experimental excisional injuries in adult female Wistar rats

2020 ◽  
Vol 38 (2) ◽  
pp. 93-100
Author(s):  
Hamilton Ricardo Alonso ◽  
Fabricio Campos Kuroda ◽  
José Roberto Passarini Junior ◽  
Juan Guzman Quispe Cabanillas ◽  
Fernanda Aparecida Sampaio Mendonça ◽  
...  
Keyword(s):  
Growth Factor ◽  
Adult Female ◽  
Wistar Rats ◽  
Collagen Fibre ◽  
Female Rats ◽  
Acupuncture Points ◽  
Type Iii Collagen ◽  
Fibroblast Proliferation ◽  
Acupuncture And Moxibustion ◽  
Female Wistar Rats

Objective: To investigate the effects of acupuncture and moxibustion on the repair of excisional skin injuries on the back of adult female Wistar rats. Methods: 90 animals were divided into three groups: C, control; A, acupuncture treatment (needled at traditional acupuncture points BL13, BL17 and ST36); M, moxibustion treatment (overlying same traditional acupuncture points). They were euthanased on days 7, 14 and 21 after injury for removal and preparation of tissue for analysis. Results: The treated groups (A and M) showed no changes regarding the structural analysis relative to the control (C) group. The total number of fibroblast cells in the A and M groups were significantly higher than those in the C group on days 14 and 21. The number of granulocytes was significantly less in the A and M groups compared with the C group on days 14 and 21. The total number of newly formed vessels increased on day 21 and was significantly higher in the A and M groups. The amount of birefringent collagen fibre detected on day 21 was significantly higher in the C group. The amount of glycosaminoglycan and hydroxyproline was similar between the groups. The amount of collagen I did not differ between the groups in any period, despite the increased amount detected over time. The amount of type III collagen did not differ between the groups but the detected amount decreased over the course of the experiment. The amount of transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in the A and M rats was similar but inferior to C rats across all experimental periods. Conclusions: Acupuncture and moxibustion stimulated fibroblast proliferation and neoangiogenesis, and extended the period of collagen fibre reorganisation in the repair of excisional injuries in adult female rats.

Post-somatostatin rebound secretion of growth hormone is dependent on growth hormone-releasing factor in unrestrained female rats

1989 ◽  
Vol 122 (2) ◽  
pp. 583-591 ◽  
Author(s):  
H. Sugihara ◽  
S. Minami ◽  
I. Wakabayashi
Keyword(s):  
Growth Hormone ◽  
Adult Female ◽  
Wistar Rats ◽  
Bolus Injection ◽  
Female Rats ◽  
Dependent Manner ◽  
Gh Secretion ◽  
Female Wistar Rats ◽  
Plasma Gh ◽  
Dose Dependent

ABSTRACT To examine the characteristics of GH secretion following the termination of the infusion of somatostatin, unrestrained adult female Wistar rats were subjected to repeated infusions of somatostatin separated by 30-min control periods. When somatostatin was infused for 150 min at a dose of 3, 30 or 300 μg/kg body wt per h, the magnitude of the rebound GH secretion increased in a dose-dependent manner. The infusion of somatostatin at a dose of 300 μg/kg body wt per h for 60, 150 or 240 min progressively augmented the size of the rebound GH secretion. When an antiserum to rat GH-releasing factor (GRF) was injected i.v. 10 min before the end of the infusion, the peak amplitude of the rebound GH secretion (300 μg/kg body wt, 150 min) was reduced to less than 20% of that of control rats. The rebound GH secretion (300 μg/kg body wt per h, 150 min) was augmented by a bolus injection of human GRF (1 μg/kg body wt). The combined effect of the end of infusion of somatostatin and a bolus injection of GRF on the amount of GH secreted was additive. The plasma GH response to GRF was completely inhibited when human GRF (3 μg/kg body wt per h) and somatostatin (300 μg/kg body wt per h) were infused simultaneously for 150 min. The magnitude of the rebound GH secretion following the termination of the co-administration was larger than that following the somatostatin infusion alone, but this rebound was not enhanced by a bolus injection of human GRF. Moreover, the amount of GH secreted was significantly less than that after the termination of somatostatin infusion plus a bolus injection of human GRF in the absence of preceding GRF administration. These results suggest that at least part of the influence of somatostatin on GH secretion is exerted at the level of the hypothalamus through modulating the release of GRF. In addition, it is inferred that the simultaneous infusion of GRF and somatostatin induces the attenuation of the GH response to GRF through a receptor effect. Journal of Endocrinology (1989) 122, 583–591

Histomorphometry and expression of Cdc47 and caspase-3 in hyperthyroid rat uteri and placentas during gestation and postpartum associated with fetal development

2007 ◽  
Vol 19 (3) ◽  
pp. 498 ◽  
Author(s):  
E. S. Freitas ◽  
E. D. Leite ◽  
C. A. Souza ◽  
N. M. Ocarino ◽  
E. Ferreira ◽  
...  
Keyword(s):  
Adult Female ◽  
Morphometric Analysis ◽  
Fetal Development ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Female Rats ◽  
Control Female ◽  
Uterine Involution ◽  
Female Wistar Rats ◽  
Medium Diameter

In two different experiments, the effects of hyperthyroidism on the histomorphometry and expression of Cdc47 and caspase-3 were evaluated in the uteri and placentas during gestation and postpartum. Fetal development was also evaluated during gestation. In the first experiment, 36 adult female Wistar rats were divided into two groups of 18 animals each: (1) hyperthyroid; and (2) euthyroid (control). Female rats were mated and killed at 7, 14 and 19 days of gestation. Uteri and placentas were weighed and subjected to histomorphometric and immunohistochemical evaluation to determine the expression of Cdc47 and caspase-3. Ovaries were also evaluated for weight and subjected to morphometric analysis. Fetuses were quantified and weighed individually. In the second experiment, 12 adult female Wistar rats were divided into two groups of six animals each: (1) hyperthyroid; and (2) euthyroid (control). Female rats were mated and killed 2 days postpartum. Uteri were evaluated in the same way as for the first experiment. Hyperthyroidism increased ovulation and conception rates without disturbing the size and viability of the fetuses. In the pregnant uteri, hyperthyroidism did not change the thickness of the layers or the expression of Cdc47 and caspase-3. However, in the placentas, hyperthyroidism increased the medium diameter of trophoblast cells, as well as the thickness and the expression of Cdc47 of spongiotrophoblast cells, at 14 days of gestation. During uterine involution, hyperthyroidism significantly increased the expression of Cdc47 and reduced the expression of caspase-3 in the uterine layers. In conclusion, hyperthyroidism increased the conception rate because of an ovulation gain, induced significant placental changes during pregnancy and, in the uterus, increased Cdc47 expression and decreased caspase-3 expression after parturition.

scholarly journals Effects of Melatonin and Epiphyseal Proteins on Fluoride-Induced Adverse Changes in Antioxidant Status of Heart, Liver, and Kidney of Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Vijay K. Bharti ◽  
R. S. Srivastava ◽  
H. Kumar ◽  
S. Bag ◽  
A. C. Majumdar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Bubalus Bubalis ◽  
Female Rats ◽  
Defense System ◽  
Body Weights ◽  
Female Wistar Rats ◽  
Liver And Kidney

Several experimental and clinical reports indicated the oxidative stress-mediated adverse changes in vital organs of human and animal in fluoride (F) toxicity. Therefore, the present study was undertaken to evaluate the therapeutic effect of buffalo(Bubalus bubalis)epiphyseal (pineal) proteins (BEP) and melatonin (MEL) against F-induced oxidative stress in heart, liver, and kidney of experimental adult female rats. To accomplish this experimental objective, twenty-four adult female Wistar rats (123–143 g body weights) were divided into four groups, namely, control, F, F + BEP, and F + MEL and were administered sodium fluoride (NaF, 150 ppm elemental F in drinking water), MEL (10 mg/kg BW, i.p.), and BEP (100 µg/kg BW, i.p.) for 28 days. There were significantlyP<0.05high levels of lipid peroxidation and catalase and low levels of reduced glutathione, superoxide dismutase, glutathione reductase, and glutathione peroxidase in cardiac, hepatic, and renal tissues of F-treated rats. Administration of BEP and MEL in F-treated rats, however, significantlyP<0.05attenuated these adverse changes in all the target components of antioxidant defense system of cardiac, hepatic, and renal tissues. The present data suggest that F can induce oxidative stress in liver, heart, and kidney of female rats which may be a mechanism in F toxicity and these adverse effects can be ameliorated by buffalo(Bubalus bubalis)epiphyseal proteins and melatonin by upregulation of antioxidant defense system of heart, liver, and kidney of rats.

NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

1974 ◽  
Vol 75 (3) ◽  
pp. 569-578 ◽  
Author(s):  
G. Buffler ◽  
S. Roser
Keyword(s):  
Wistar Rats ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Cycle Duration ◽  
Follicular Growth ◽  
The Third ◽  
Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

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