Histological, Micrometrical and Histochemical Studies on the Testes of Large White Yorkshire Pig (Sus scrofa domesticus)

2020 ◽  
Author(s):  
Thokchom Shitarjit Singh ◽  
P. C. Kalita ◽  
O. P. Choudhary ◽  
A. Kalita ◽  
P. J. Doley
Keyword(s):  
Connective Tissue ◽  
Basement Membrane ◽  
Tunica Albuginea ◽  
Periodic Acid ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Moderate Activity ◽  
Large White ◽  
Periodic Acid Schiff ◽  
Strong Affinity

The study has been conducted on the testes of 10 adult Large White Yorkshire pig (1.5-2) years of age. The testis was surrounded by a capsule made up of dense irregular connective tissue comprising three layers viz., outer visceral layer of tunica vaginalis, middle tunica albugenia and inner tunica vasculosa. The connective tissue trabeculae were extended from the capsule and divided the parenchyma of the testis into number of lobules and consisted of collagen, elastic and reticular fibers along with rete testis and blood vessels. The Periodic Acid–Schiff shows strong affinity in basement membrane of seminiferous tubules and tunica albuginea with a moderate activity in Leydig cells. The Alcian blue shows strong affinity in basement membrane of seminiferous tubule and weak activity was observed in tunica albuginea and interstitial tissue. The different histomorphometrical parameters with regard to thickness of testicular capsule, diameter and height of seminiferous tubules were found to be higher in the left testicle.

scholarly journals Morphology of the testes and epididymal ducts in the pampas cat Leopardus colocolo (Molina, 1782)

2016 ◽  
Vol 36 (10) ◽  
pp. 1014-1020
Author(s):  
Mahmoud Mehanna ◽  
◽  
André L.S. Ferreira ◽  
Adelina Ferreira ◽  
Regina C.R. Paz ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Basement Membrane ◽  
Tunica Albuginea ◽  
The Body ◽  
Seminiferous Tubules ◽  
Secretory Cells ◽  
Interstitial Tissue ◽  
Basal Cells ◽  
Myoid Cells ◽  
Mato Grosso

ABSTRACT: The pampas cat Leopardus colocolo (Molina, 1782) is a species of the Felidae family, widely distributed in South America, included on CITES Appendix II and classified as Near Threatened on the IUCN Red List, with population trend decreasing. Based on this information, the objective of this study is to describe morphologically the testes and epididymal ducts of pampas cat. The animal, coming from the Federal University of Mato Grosso Zoo, Brazil, had died after anesthesia procedure and the male reproductive system was dissected to collect the testicles. The samples taken were fragmented and histologically examined. From the microscopic analysis of the testes were identified: vaginal and tunica albuginea, formed by dense connective tissue modeled with large amount of collagen fibers. The tunica albuginea fibrous septa emits into the body. The seminiferous tubules are coiled and coated internally by spermatogenic epithelium consisting of Sertoli cells, surrounded by a basement membrane in the presence of myoid cells. The interstitial tissue between the seminiferous tubules, is composed of loose connective tissue, blood and lymph vessels, and Leydig cells in polyhedral shape. The epididymal ducts showed pseudostratified columnar epithelium with secretory cells of which stereocilia design, situated on a basement membrane filled by myoid cells. This epithelium has principal and basal cells, the main cell design stereocilia toward the lumen of the epididymal duct.

scholarly journals Granulomatous Epididymitis Related to Rhodotorula glutinis Infection in a Dog

1995 ◽  
Vol 32 (6) ◽  
pp. 716-718 ◽  
Author(s):  
K. Kadota ◽  
K. Uchida ◽  
T. Nagatomo ◽  
Y. Goto ◽  
T. Shinjo ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Sertoli Cells ◽  
Periodic Acid ◽  
Rhodotorula Glutinis ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Cell Debris ◽  
Periodic Acid Schiff ◽  
Microbiological Characteristics ◽  
Cut Surface

A 4-year-old, male Great Dane dog developed severe swelling of the scrotum on 9 December 1991, and the testes and epididymides were removed surgically on 12 December 1992. The cut surface of the epididymides consisted of hard connective tissue and several small abcesses with slight hemorrhage. Histopathologically, the seminiferous tubules in the testes had only a few spermatogenic cells, but Sertoli cells were well preserved. Both epididymides consisted entirely of a proliferation of fibrous connective tissue, and only a few ducts deferens containing cell debris, neutrophils, and macrophages in the lumina were present. In all lesions of the epididymides, the macrophages contained periodic acid–Schiff– and Grocott's silver–positive round granules, 5-8 μm in diameter. Microbiologically, smooth salmon-pink colonies consisting of ovoidal yeast, about 10 μm in diameter, were isolated from the samples of epididymides but not from those of the testes. The isolated yeast had microbiological characteristics of Rhodotorula glutinis. From these observations, we diagnosed the present case as granulomatous epididymitis due to Rhodotorula infection.

The Haemocytes and Connective Tissue Formation in an Insect, Rhodnius Prolixus (Hemiptera)

1956 ◽  
Vol s3-97 (37) ◽  
pp. 89-98
Author(s):  
V. B. WIGGLESWORTH
Keyword(s):  
Connective Tissue ◽  
Basement Membrane ◽  
Blood Cell ◽  
Periodic Acid ◽  
Rhodnius Prolixus ◽  
Ground Substance ◽  
Muscle Fibres ◽  
Tissue Formation ◽  
Periodic Acid Schiff ◽  
Positive Material

The amoebocytes, which form the most abundant type of blood-cell in Rhodnius, contain rounded, oval, or rod-shaped inclusions which stain by the periodic acid/Schiff method. These are believed to be neutral mucopolysaccharides. The amoebocytes apply themselves to the basal membranes, which likewise are PAS-positive, and appear to contribute to these membranes by discharging their contents. They insinuate themselves into the developing muscles and give rise to the sheaths of connective tissue by which the muscle fibres are surrounded. And they collect around deposits of injected Indian ink, producing similar sheets of PAS-positive material, presumably mucopolysaccharide. The chitinous endocuticle is PAS-negative (except in a few special regions such as the neck and the conjunctival membranes of the limbs). It becomes positive during digestion by the moulting fluid. The tracheae react similarly. Other PAS-positive structures which are not produced by the amoebocytes are the striated border of the mid-gut, the basement membrane of the gut, the perilemma around the ganglia and nerves, and the ground substance within the ganglia.

scholarly journals Resveratrol Ameliorates Testicular Histopathology of Mice Exposed to Restraint Stress

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 743 ◽  
Author(s):  
Sheeraz Mustafa ◽  
Quanwei Wei ◽  
Wael Ennab ◽  
Zengpeng Lv ◽  
Korejo Nazar ◽  
...  
Keyword(s):  
Restraint Stress ◽  
Immobilization Stress ◽  
Tunica Albuginea ◽  
Periodic Acid ◽  
Cell Number ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Testis Weight ◽  
Periodic Acid Schiff ◽  
Luminal Diameter

We evaluated immobilization stress and resveratrol supplementation in immature male mice at 30 days of age for 15 consecutive days. Fifty Swiss mice were divided into five groups (10 mice each): Controls, restraint stress (RS), restraint stress + vehicle (RS + V), RS + 2 mg/kg, and RS + 20 mg/kg. We determined results on the basis of hematoxylin and eosin (H&E), “Periodic acid-Schiff” staining, and TUNEL assay. The results indicated that immobilization stress significantly decreased body weight, testis weight, and water/food intake compared to the control; while resveratrol ameliorated these effects. The quantitative histologic evaluation of the seminiferous tubule diameter, luminal diameter, area of seminiferous tubules, area of tubule lumen, epithelial height, Leydig cell number, and the width of the tunica albuginea were similarly decreased after exposure to RS. These parameters recovered back to normal in the RS + 2 mg/kg group. The development of spermatogenesis was significantly delayed in the RS, RS + V, and RS + 20 mg groups based upon our evaluation score system. However, we observed no significant differences in the RS + 2 mg group compared with the control group. The number of TUNEL-positive cells also significantly decreased in the RS + 2 mg/kg group. In conclusion, we found that the administration of 2 mg/kg was an effective dose against immobilization stress in mice.

HISTOLOGICAL EFFECTS OF 3-TRIFLUORMETHYL-4-NITROPHENOL (TFM) ON LARVAL LAMPREY AND TROUT

1963 ◽  
Vol 41 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Ronald M. Christie ◽  
Helen I. Battle
Keyword(s):  
Connective Tissue ◽  
Prolonged Exposure ◽  
Periodic Acid ◽  
Secretory Activity ◽  
The Body ◽  
Salmo Gairdneri ◽  
Periodic Acid Schiff ◽  
Sinusoidal Dilatation ◽  
Gill Filaments ◽  
Red Coloration

Larvae of the lamprey, Entosphenus lamottei (Le Sueur), and rainbow trout, Salmo gairdneri Richardson, were exposed to the sodium salt of 3-trifluormethyl-4-nitrophenol (TFM) in concentrations of 0.75 p.p.m., 3.00 p.p.m., and 6.00 p.p.m. Microscopic examination of changes induced in the gills, liver, cloacal region, and musculature were made on 7-micron sections stained with Harris' haematoxylin and Bowie's eosin, and in the gill region with periodic acid Schiff reagent. A comparison of the degree of the effects in the two species was made by planimetry of the vascular, cellular, and edematous areas from enlarged drawings of sections.Upon exposure to lethal concentrations of TFM, the body of the larval lamprey becomes distended at the pharyngeal level and heavy cords of mucus emerge from the external gill clefts. A deep red coloration is evident in the pharyngeal region consequent upon vasodilatation of the arterioles and capillaries of the gill filaments. Trout exhibit a similar vasodilatation of the gills together with increased mucous secretion. Edema in the connective tissue between the respiratory epithelium and the vascular endothelium is induced in both species. After prolonged exposure to TFM, the mucous cells in the lining of the branchial chamber and covering the tips of the gill filaments are actively discharging their secretions or completely spent.Certain effects induced by TFM in the larval lamprey are not evident in the trout. The cloacal region takes on a deep red coloration due to dilatation of the venous sinuses and the liver becomes reddish because of sinusoidal dilatation. Extensive edema of the fibrous connective tissue of the skeletal musculature is characteristically present. A slightly increased secretory activity of mucous-secreting cells may occur in the epidermis.With the techniques employed in this study, there was no evidence in either species of cytological or histological changes in the nervous tissue, cardiac musculature, notochord, alimentary canal (including the haemopoietic typhlosole of the lamprey), or mesonephros.

scholarly journals IMMUNOLOGIC STUDIES OF HEART TISSUE

1961 ◽  
Vol 113 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Melvin H. Kaplan ◽  
Frederick D. Dallenbach
Keyword(s):  
Heart Disease ◽  
Connective Tissue ◽  
Gamma Globulin ◽  
Fluorescent Antibody ◽  
Periodic Acid ◽  
Heart Tissue ◽  
Periodic Acid Schiff ◽  
Schiff Reagent ◽  
Points Of View ◽  
Rheumatic Heart

Using fluorescent antibody methods, deposits of bound gamma globulin, as determined in unfixed washed sections of auricular appendages from rheumatic hearts, were noted in a significant number (18 per cent) of 100 specimens studied. Such deposits were observed in myofibers, sarcolemma, interstitial connective tissue, and vessel walls. Albumin and fibrin were generally found absent from these sites. Control hearts from normal and pathologic material, including postmortem and biopsied specimens, in general, did not reveal such deposits. These various tissue sites which contained bound gamma globulin frequently exhibited evidence of alteration as indicated both by enhanced affinity for eosin and by strongly positive reaction with the periodic acid-Schiff reagent, and appeared comparable in some cases to "fibrinoid." Bound gamma globulin was not observed in cellular or stromal components of Aschoff lesions, nor was the occurrence of Aschoff lesions correlated with presence of bound gamma globulin. It is suggested that deposition of gamma globulin and the eosinophilic alteration associated with such deposition are related to certain of the pathologic changes of rheumatic heart disease. The nature of such deposits of gamma globulin was considered from immune and non-immune points of view.

scholarly journals The Potential of Light Microscopic Features of the Oral Mucosa in Predicting Post-mortem Interval

2021 ◽  
Vol 21 (1) ◽  
pp. e34-41
Author(s):  
Jagganath Patro ◽  
Swagatika Panda ◽  
Neeta Mohanty ◽  
Uma S. Mishra
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Buccal Mucosa ◽  
Periodic Acid ◽  
Post Mortem ◽  
Periodic Acid Schiff ◽  
Post Mortem Interval ◽  
Cellular Changes ◽  
Microscopic Evaluation ◽  
Microscopic Features

Objectives: The post-mortem interval (PMI) refers to the amount of time elapsed between death and discovery of the body. This study aimed to evaluate light microscopic cellular changes in the oral mucosa and identify the potential of this method for predicting PMI. Methods: This prospective study was conducted between July 2016 and January 2018 at the Institute of Dental Sciences, Siksha ‘O’ Anusandhan University, Bhubaneswar, India. A total of 150 post-mortem (including 75 gingival and 75 buccal mucosa samples) and 40 ante-mortem (including 20 gingival and 20 buccal mucosa samples) tissue samples were compared using haematoxylin and eosin, periodic acid-Schiff (PAS) and van Gieson stains. Microscopic changes in the epithelium and connective tissue were categorised according to PMI stage as early (<12.5 hours since death), intermediate (12.5–20.5 hours since death) or late (>20.5 hours since death). Results: Most epithelial cellular changes occurred early, except for arc-shaped nuclei and epithelial shredding which were intermediate and late changes, respectively. However, microscopic changes in the connective tissue were only observable at ≥12.5 hours. There was a progressive decrease in intensity in van Gieson stains and an increase in intensity in PAS stains as PMI increased. Several microscopic features were found to be significant predictors of PMI including epithelial homogenisation, cytoplasmic vacuolation, nuclear degeneration, arc-shaped nuclei, chromatin clumping, red blood cell clumping and lysis, melanin incontinency, myofibril degeneration, salivary gland acini degeneration and epithelial connective tissue separation (P <0.050 each). Conclusion: These findings indicate that microscopic evaluation of the oral mucosa may be helpful for PMI prediction.   KEYWORDS Post-mortem Changes; Light Microscopy; Oral Mucosa; Epithelial Cells; Lamina Propria; Salivary Glands; Histocytochemistry; Periodic Acid-Schiff Reaction; India.

Acute Exposure to the Neonicotinoid Insecticide Imidacloprid of Zebrafish (Danio rerio) Gonads: A Histopathological Approach

2021 ◽  
Vol 57 ◽  
pp. 23
Author(s):  
Cansu Akbulut
Keyword(s):  
Interstitial Fibrosis ◽  
Periodic Acid ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Periodic Acid Schiff ◽  
Histological Techniques ◽  
Zona Radiata ◽  
Structural Deterioration ◽  
Neonicotinoid Insecticide

Neonicotinoids are the new class of insecticides that are high target specificity to insects. Imidacloprid is a neonicotinoid that is the most widely used insecticide in the world. As a result of its widespread use in agriculture, imidacloprid interferes with the aquatic system and threatens the aquatic environment. In this study, an investigation of the histopathological effects of imidacloprid on zebrafish gonads was aimed. Zebrafish were exposed to 9.5 mg/L, 19 mg/L, and 38 mg/L of imidacloprid for 5 days, considering the 96 h LC50 value. After dissecting the gonadal tissues, routine histological techniques were applied, and the tissues were stained with Periodic Acid-Schiff (PAS), Toluidine Blue (TB), and Haematoxylin and Eosin (H&E). Sections were examined under a light microscope. While normal gonad histology was observed in the control group, histopathological alternations such as degeneration and union in the seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, and interstitial fibrosis were detected in testicular tissue of the experimental groups. In the ovarian tissues of the experimental groups, structural deterioration in oocytes, autolysis, increase in the number of atretic oocytes, vacuolization in cortical alveoli, thickening and curling in the zona radiata, and opening in the perifollicular layers were detected.

scholarly journals SOME HISTOCHEMICAL OBSERVATIONS ON TRANSITIONAL EPITHELIUM OF MAN

1967 ◽  
Vol 15 (8) ◽  
pp. 475-481 ◽  
Author(s):  
BENITO MONIS ◽  
HOWARD D. DORFMAN
Keyword(s):  
Urinary Tract ◽  
Basement Membrane ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Normal Component ◽  
Free Cell ◽  
Transitional Epithelium ◽  
Colloidal Iron ◽  
Periodic Acid Schiff ◽  
Mucous Coat

This report deals with a histochemical characterization of transitional epithelium of the urinary tract of man. The cytoplasm of cells of the innermost, luminal layer is distinctly stained by the periodic acid-Schiff procedure. Enhanced staining of the luminal border of these cells suggests the presence of a differentiation of the luminal surface ( surface mucous coat) which is selectively stained by Alcian Blue and colloidal iron. The alcianophilic surface mucous coat is seen in the innermost layer of transitional epithelial cells of the urinary tract extending from the area cribrosa down to the bladder. This indicates the presence of an acid mucinous component. Digestion with neuraminidase according to the procedure of Spicer and Warren demonstrates the presence of N-acetylneuraminic acid (sialic acid) in the free cell surface of the luminal layer of transitional epithelium of man. There is abundant glycogen in transitional epithelium of man. A very characteristic basement membrane underlies transitional epithelium. It reacts with periodic acid-Schiff and often with Alcian Blue. The neuraminidase digestion does not alter the staining of basement membrane with Alcian Blue. This suggests the presence of a sialidase-resistant form of sialomucin or a sulfomucin in basement membrane. The presence of periodic acid-Schiff-positive, nondigestible granules closely corresponds to acid phosphatase-reactive granules, presumably lysosomes. Within the transitional epithelium there were lacunae which contained abundant sialomucins. These structures have been described in old textbooks of histology but do not seem to be a normal component of transitional epithelium. The significance of these structures is unknown.

scholarly journals Three-dimensional analysis of seminiferous tubules and spermatogenic waves in mice

Reproduction ◽  
2017 ◽  
Vol 154 (5) ◽  
pp. 569-579 ◽  
Author(s):  
Hiroki Nakata ◽  
Takahiro Sonomura ◽  
Shoichi Iseki
Keyword(s):  
High Performance ◽  
Average Length ◽  
Periodic Acid ◽  
3D Structure ◽  
Three Dimensional ◽  
Rete Testis ◽  
Seminiferous Tubules ◽  
Periodic Acid Schiff ◽  
Adult Mice ◽  
Branching Points

The aim of the present study was to reconstruct seminiferous tubules and analyze spermatogenic waves in seminiferous epithelia in developing and adult mice using serial paraffin sections and high-performance three-dimensional (3D) reconstruction software. By labeling the basement membrane of seminiferous tubules with fluorescent immunohistochemistry or periodic acid-Schiff-hematoxylin staining, all seminiferous tubules were reconstructed in 9 testes from 9 different mice, 3 each at 0, 21 and 90 days (adult) postpartum. The 3D structure of seminiferous tubules, including the number and length of tubules as well as the number of connections with the rete testis, branching points and blind ends, was assessed accurately. Although tubules showed marked variations among individual mice, their overall structure was regular and retained from newborn to adult mice. Some seminiferous tubules contained inner portions running distant from the testis surface. In a representative testis at 21 days, the sites at which spermatids initially occurred were examined by labeling acrosomes and were found to be preferentially distributed in the upper and medial portions of the testis close to the rete testis. In a representative adult testis, 76 complete waves with an average length of 16.9 mm were found and their directions were analyzed. The methods used in the present study will be useful for investigating the structure and function of seminiferous tubules in mice and humans under normal and pathological conditions, such as infertility.

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