​POU1F1 Gene Polymorphism and its Associations with Body Weight of Osmanabadi Goat

2021 ◽  
Author(s):  
Vikrant D. Pawar ◽  
Mahadeo P. Sawane ◽  
Aakash Y. Doiphode
Keyword(s):  
Body Weight ◽  
Gene Polymorphism ◽  
Age Groups ◽  
Restriction Enzymes ◽  
Nucleotide Position ◽  
Pou1f1 Gene ◽  
Body Weights ◽  
Pcr Rflp ◽  
Indigenous Goat ◽  
Selection Of

Background: Osmanabadi is the premium meat type goat breed of Maharashtra, known for its adaptability and reproductive efficiency. Identification of superior germplasm and incorporating them into breeding programme is the need of hour for augmenting productivity of Osmanabadi as well as other indigenous goat breeds. Hence, the present research was aimed to study Pituitary Specific Transcription Factor I (POU1F1) gene polymorphism and its associations with body weight of Osmanabadi goat. Methods: Genomic DNA was extracted from 217 blood samples of randomly selected Osmanabadi goats. The 450 bp fragment of POU1F1 gene encompassing exon-6 and its flanking region (3’UTR) was PCR amplified. The PCR amplicon was subjected to RFLP using AluI and PstI restriction enzymes to identify polymorphism at nucleotide position 174 (T greater than C) in exon 6 and at nucleotide position 110 (T greater than C) in the 3’UTR of POU1F1 gene, respectively. The association of the observed allelic variants / genotypes with body weight of the animals was performed using One-way ANOVA. Result: The AluI PCR-RFLP of 450 bp amplicon of POU1F1 gene in Osmanabadi goats revealed polymorphism with three different genotypes viz., ‘CC’, ‘TT’ and ‘TC’. The observed frequencies for TT, TC and CC genotypes were 0.604, 0.355 and 0.041 respectively. The frequencies of T and C alleles were found to be 0.781 and 0.219, respectively. However, PstI PCR-RFLP revealed monomorphic 3’UTR of POU1F1 gene with single TT genotype. We observed significant differences (P less than 0.01) between various genotypes at AluI exon-6 locus of POU1F1 gene and body weights at birth, 3 and 6 months of Osmanabadi goat. Highest body weight was recorded for TT genotype followed by TC and CC genotypes in all three age groups. Our findings indicate that the AluI PCR-RFLP locus of the exon-6 of POU1F1 gene can be used as a potent molecular marker for selection of superior stock of Osmanabadi goats.

Prediction of body weights of Nellore sheep under field condition

2015 ◽  
Author(s):  
M. Rani ◽  
B. Ekambaram ◽  
B. Punya Kumari
Keyword(s):  
Body Weight ◽  
Body Length ◽  
Age Groups ◽  
The Body ◽  
Coefficient Of Determination ◽  
Regression Equations ◽  
Body Weights ◽  
Chest Girth ◽  
Males And Females ◽  
Nellore Sheep

Data on 1350 Nellore sheep of 2, 4, 6 and 8-teeth age, reared under field conditions in 12 mandals of Chittoor district of Andhra Pradesh were utilized for development of prediction equations and study the phenotypic association among body measurements and body weights. The coefficients of correlation between body weight with the height at withers, chest girth, paunch girth, hip width and body length were positive and high in magnitude in both males and females in majority of the age groups studied. Step-down regression equations were fitted to predict the body weight based on biometrical measurements at different ages. The height at withers, chest girth, paunch girth, hip width and body length have contributed significantly to the expression of body weights at the majority of the ages studied. High coefficient of determination (R2) value was observed in males at 6 and 8-teeth age as 88 per cent, while in females 50 per cent at 2-teeth age.

A DdeI PCR-RFLP detecting genetic variation of goat POU1F1 gene

2007 ◽  
Vol 87 (1) ◽  
pp. 13-14 ◽  
Author(s):  
X. Y. Lan ◽  
C. Y. Pan ◽  
H. Chen ◽  
C. Z. Lei
Keyword(s):  
Genetic Variation ◽  
Gene Polymorphism ◽  
Key Words ◽  
Milk Yield ◽  
Dairy Goat ◽  
Pou1f1 Gene ◽  
Pcr Rflp ◽  
Silent Allele

We described a DdeI PCR-RFLP method for detecting silent allele at goat POU1F1 locus: p.S241S. Frequencies of D1 allele varied from 0.600 to 1.000 in eight Chinese native breeds. Association of DdeI RFLP genotypes with milk yield of dairy goat was significant (P < 0.05). Key words: Goat, POU1F1 gene, polymorphism, association, milk yield

scholarly journals Relationship between egg weight, hatching weight and subsequent body weight in the Japanese quail

2020 ◽  
Vol 47 (1) ◽  
pp. 19-23
Author(s):  
V. O. Chimezie ◽  
A. A. Ademola ◽  
O. I. Alli ◽  
A. E. Jubril ◽  
B. O. Josiah
Keyword(s):  
Body Weight ◽  
Japanese Quail ◽  
Coefficient Of Determination ◽  
Egg Weight ◽  
Body Weights ◽  
The Mean ◽  
The University ◽  
Large Groups ◽  
The Relationship ◽  
Selection Of

An experiment to determine the relationship between egg weight, hatch weight and subsequent body weight of the Japanese quail was conducted. A total of 607 eggs collected over 6 days from an existing flock at the Animal Pavilion of the University of Ilorin were used for the experiment. Eggs were individually numbered, weighed and grouped into small (S) medium (M) and large (L) sized eggs based on the mean and standard deviation of the eggs. Mean egg weight were correlated and regressed with mean hatch weight and subsequent weight. Average egg weights were 10.23± 0.84g, 8.49± 0.72g and 6.94± 0.93g for the large, medium and small group respectively. The result showed relationship between egg weight and hatch weight were positive and highly significant (P < 0.01) with values of 0.973, 0.977 and 0.967 for small, medium and large groups, respectively. Relationship between hatch weights and weekly body weights were also positive and significant (P<0.01) for the medium and large groups only but decreased with age. Linear regression equation showed a significant coefficient of determination for weekly body weight using the values for small, medium and large egg weight values. The results of the study showed that egg weight can be reliably used for estimating body weight in Japanese quail and may be used as criteria for early selection of Japanese quail for body weight.

scholarly journals PUBERAL DEVELOPMENT IN THE NIGERIAN DWARF SHEEP

2021 ◽  
Vol 7 (1) ◽  
pp. 71-76
Author(s):  
B. I. Orji ◽  
J. Steinbach
Keyword(s):  
Body Weight ◽  
Reproductive Tract ◽  
Age Groups ◽  
Reproductive Organs ◽  
The Body ◽  
Endocrine Function ◽  
Physiological Age ◽  
Body Weights ◽  
Pituitary Glands ◽  
Ram Lambs

Eleven ram lambs, all born between June and July (rainy season) were slaughtered at four age groups — 75, 100, 125 and 150 days. The body weights of animals prior to slaughter, the weights of the testes, the epididymis, the vesicular glands, the ampullae, the adrenals, the thyroids and the pituitary glands were determined and subjected to a step-wise multiple regression analysis using age or body weight as the independent variable. The weights of the reproductive organs – testes, epididymides, ampullae, vesicular glands - showed significantly higher correlation with the body weight than with the age, thus emphasizing the greater importance of the physiological age over the chronological age in the development of the body organs. The highest growth rate of the organs of the reproductive tract occurred during puberty and thus coincided with the enhanced endocrine function observed in ram lambs at this stage.

The effects of inbreeding, maternal handicap, and range in age on 10 fleece and body characteristics in Merino rams and ewes

1967 ◽  
Vol 18 (4) ◽  
pp. 689 ◽  
Author(s):  
J Lax ◽  
GH Brown
Keyword(s):  
Body Weight ◽  
Positive Relationship ◽  
Age Groups ◽  
Fibre Diameter ◽  
Regression Coefficients ◽  
Multiple Births ◽  
Multiple Birth ◽  
Skin Area ◽  
Usual Practice ◽  
Body Weights

Estimates have been made of the influence on 10 fleece and body characteristics of dam's phenotype, range in age of offspring, inbreeding of offspring and dam, type of birth of offspring, and age of dam. The data came from rams 10–11 months old and ewes 15–16 months old, which had previously been shorn as weaners; the animals belong to an unselected control flock run at Cunnamulla, south-western Queensland. The dam's phenotype showed a high positive relationship with that of her offspring in all 10 characteristics, the values of the regression coefficients confirming the levels of heritability previously estimated on the same flock but without removing all the effects included in the present analysis. Younger animals in a group had lower greasy and clean wool weights and body weights, even at 15–16 months, but the effects were slight, except for body weight. For each 1% increase in the offspring's own level of inbreeding, the regression coefficients for rams and ewes respectively were -0.042 and –0,051 lb for greasy wool weight, –0.025 and –0.025 1b for clean wool weight, and –0.431 and –0.541 1b for body weight. These figures represent a decrease of 5–10% in clean wool weight and 8–10% in body weight for the progeny of half-sib matings. Inbreeding of the dam had a negligible effect on the characteristics examined. Animals born in multiple births in this environment suffer penalties which were still evident up to 15–16 months of age, resulting in both sexes in lower wool and body weights, lower wrinkle scores, fewer fibres per unit skin area, and fewer crimps per inch of staple. Fibre diameter was slightly higher for the multiple birth animals, but �he signs for the effects on other characteristics differed between sexes. The handicaps for rams and ewes respectively were 9 and 5% for clean wool weight and 7 and 5% for body weight. Dams were separated into four age groups (2, 3, 4–7, and 8–10 years). Greasy and clean wool weights and wrinkle score rose for offspring of both sexes to a peak at 4–7-year-old dams, then fell slightly. Body weight reached a peak at 3 years for rams and 4–7 years for ewes, while fibre diameter and staple length reached a peak at 3 years for ewes and 4–7 years for rams. In no case was the fall for the older dam age groups sufficient to invalidate the usual practice of adjusting only the progeny of 2-year-old dams. Other characteristics showed no consistent pattern.

scholarly journals Melanocortin 4 receptor (Mc4r) gene polymorphism and its association with body weights of some breeds of rabbit

2020 ◽  
Vol 47 (2) ◽  
pp. 1-7
Author(s):  
O. H. Osaiyuwu ◽  
U. F. O. Bolaji ◽  
O. A. Adeyinka ◽  
M. O. Akinyemi ◽  
A. E. Salako
Keyword(s):  
Body Weight ◽  
New Zealand ◽  
Gene Polymorphism ◽  
Rflp Analysis ◽  
The Body ◽  
Chi Square ◽  
Mc4r Gene ◽  
Fta Cards ◽  
Melanocortin 4 Receptor ◽  
Pcr Rflp

In livestock production, traditional methods of selection have always been the way to go. However, with the advent of genomics techniques, methods such as PCR-RFLP is been employed to identify single nucleotide polymorphism of likely candidate genes useful for livestock selection and improvement. Therefore, the aim of this study was to investigate the association of Melanocortin 4 Receptor (MC4R) gene polymorphism with the body weight ofrabbits. Seventy-four rabbits were used for this study consisting of six breeds; 26 Chinchilla, 3 Californian, 11 Dutch, 4 English Spot, 10 New Zealand White and 20 Fauve de Bourgogne (FDB) breeds. Blood samples were collected from the animals with needle and syringe and transferred unto FTA cards and stored away from light. Body weight measurements on the animals were recorded from 2 weeks to 20 weeks. PCR-RFLP analysis produced threegenotypes AA, AG, GG with genotype frequency of 0.14, 0.69 and 0.18 respectively. Allele frequency 0.48 and 0.52 for allele A and G respectively was obtained. Chi-square test showed that the population was not in Hardy-Weinberg equilibrium. Association analysis between the MC4R “c.101G>A” SNP and body weight of rabbit was tested using GLM procedure of SAS programme. Genotype GG had higher body weight (p<0.05) at 12, 16 and 20 weeks inthe Dutch rabbits while genotype AA recorded a higher (p<0.05) body weight value at 12 weeks in New Zealand White rabbits. More so, female Dutch rabbits had higher (p<0.05) mean weight than the males at 12, 16 and 20 weeks of age. Male New Zealand White breeds recorded higher (p<0.05) mean weight than the female animals. The result of the study showed that MC4R “c.101G>A” SNP was not associated with the body weight in the studied rabbit population, although, genotype AA had higher mean weight values at 12, 16 and 20 weeks of age than genotypes AG and GG. 

scholarly journals Computer-assisted selection of restriction enzymes for rrs genes PCR-RFLP discrimination of rhizobial species

1998 ◽  
Vol 30 (Suppl 1) ◽  
pp. S297 ◽  
Author(s):  
Marc Neyra ◽  
Bouchaib Khbaya ◽  
Philippe de Lajudie ◽  
Bernard Dreyfus ◽  
Philippe Normand
Keyword(s):  
Restriction Enzymes ◽  
Computer Assisted ◽  
Pcr Rflp ◽  
Rhizobial Species ◽  
Selection Of

scholarly journals Characterization of Maasai Goats in Extensive Production System in Northern Tanzania: Description of Phenotype, Reproductive and Productive Performance

2020 ◽  
Vol 13 (1) ◽  
pp. 99
Author(s):  
Emil Hyera ◽  
Athumani S. Nguluma ◽  
Zabron C. Nziku ◽  
Eligy J. M. Shirima ◽  
Maria-Salome H. Mashingo ◽  
...  
Keyword(s):  
Body Weight ◽  
Coat Color ◽  
Age Groups ◽  
Head Length ◽  
Head Width ◽  
Lactation Length ◽  
Northern Tanzania ◽  
Indigenous Goat ◽  
Productive Traits ◽  
Main Effects

The study was designed to offer information on phenotype, reproductive and productive features of Maasai goats for amelioration in breeding programs utilizing these animals. Live measurements and qualitative traits were collected from 75 bucks and 165 does. A detailed survey was used to acquire information on reproductive and productive traits. Data were analyzed using descriptive statistics and General Linear Model procedures for age and sex as main effects. The results revealed that body weight, heart girth, chest depth, rump width, head length, head width and horn length differed (p &lt; 0.05) among age groups. Body weight increased from young to old age and ranged from 25.83 kg to 30.34 kg. Body length, heart girth, head length, head width and cannon bone length were significantly (p &lt; 0.05) higher in bucks than does. Plain white was the foremost coat color manifested. Nearly, 76% of bucks and 78.2% of does had straight short hairs. Beards were in 80% of bucks and 9.1% of does. All goats had short and erect ears; whereas, more than 88% had horns, 48% of the horns were curved, 33.1% lateral and 18.1% straight. Almost 76% and 83.6% of the horns in bucks and does, respectively, were pointing backward. In both sexes, facial profile was mainly concave, back profile was straight and wattles were absent. Twinning rate, age at sexual maturity, age at first kidding, kidding interval and lactation length were averaged at 8.7%, 11 months, 16.7 months, 7.7 months and 82.3 days; whereas, pre weaning kid survival rate was 77.1% and 79.9% for dry and rainy season, respectively. The strain was comparatively similar to most African indigenous goat populations. Thus, the strain can be utilized through selection for the traits preferred for the arid and semi-arid tropics.

scholarly journals Effects of aging, phenotype and carbohydrate feeding on caloric efficiency and adiposity in the LA/Ntul//-cp (corpulent) rat

2021 ◽  
Vol 11 (1) ◽  
pp. 5-11
Author(s):  
Orien L Tulp
Keyword(s):  
Body Weight ◽  
Sucrose Solution ◽  
Age Groups ◽  
Caloric Intake ◽  
Energy Utilization ◽  
Caloric Efficiency ◽  
Carbohydrate Feeding ◽  
Body Weights ◽  
Obese Phenotype ◽  
Effects Of Aging

Obesity develops in the obese phenotype of the LA/Ntul//-cp (corpulent) specific pathogen-free rat strain by 5 to 6 weeks of age. Groups [n=12 -20 rats/phenotype] of female congenic lean and obese LA/Ntul//-cp (corpulent) rats were fed ad libitum standardized Purina diets for 4, 14, or 24 months or the same diet plus a 16% (w/v) sucrose solution supplement from 12 weeks of age, and measures of body weight, caloric intake, and caloric efficiency (CE) determined at each age group. Body weights of lean animals remained similar at all ages studied, while body weights of obese phenotype were significantly greater than their lean littermates at each age studied. The sucrose supplement was without significant effect on final body weights in the lean phenotypes at all ages studied (p=n.s.) but were associated with greater body weights at ages 4, 14 and 24 months of age in the obese phenotype (p=<0.05). CE was determined as the ratio of kcal/gram of body weight per day remained relatively constant in lean animals throughout the age range, but CE was more efficient in the obese phenotype at all ages studied and became progressively more efficient with the sucrose supplement feeding with increasing age. The results of this study indicate that CE is associated with the predisposition for the development of obesity in the obese phenotype of this strain and likely implicates multiple metabolic factors that contribute to a greater efficiency of energy utilization and or energy conservation in the obese than in the lean phenotype of this strain, and the metabolic impact of added sucrose was associated with an additive impact on the CE of weight gain and adiposity in the obese phenotype of this congenic rodent strain

scholarly journals Derived Polymorphic Amplified Cleaved Sequence (dPACS): A Novel PCR-RFLP Procedure for Detecting Known Single Nucleotide and Deletion–Insertion Polymorphisms

2019 ◽  
Vol 20 (13) ◽  
pp. 3193 ◽  
Author(s):  
Shiv Shankhar Kaundun ◽  
Elisabetta Marchegiani ◽  
Sarah-Jane Hutchings ◽  
Ken Baker
Keyword(s):  
Gel Electrophoresis ◽  
Restriction Site ◽  
Restriction Enzymes ◽  
Nucleotide Polymorphisms ◽  
Acetyl Coa Carboxylase ◽  
Wild Type ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Broadleaf Species ◽  
Selection Of

Most methods developed for detecting known single nucleotide polymorphisms (SNP) and deletion–insertion polymorphisms (DIP) are dependent on sequence conservation around the SNP/DIP and are therefore not suitable for application to heterogeneous organisms. Here we describe a novel, versatile and simple PCR-RFLP procedure baptised ‘derived Polymorphic Amplified Cleaved Sequence’ (dPACS) for genotyping individual samples. The notable advantage of the method is that it employs a pair of primers that cover the entire fragment to be amplified except for one or few diagnostic bases around the SNP/DIP being investigated. As such, it provides greater opportunities to introduce mismatches in one or both of the 35–55 bp primers for creating a restriction site that unambiguously differentiates wild from mutant sequences following PCR-RFLP and horizontal MetaPhorTM gel electrophoresis. Selection of effective restriction enzymes and primers is aided by the newly developed dPACS 1.0 software. The highly transferable dPACS procedure is exemplified here with the positive detection (in up to 24 grass and broadleaf species tested) of wild type proline106 of 5-enolpyruvylshikimate-3-phosphate synthase and its serine, threonine and alanine variants that confer resistance to glyphosate, and serine264 and isoleucine2041 which are key target-site determinants for weed sensitivities to some photosystem II and acetyl-CoA carboxylase inhibiting herbicides, respectively.

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