Seasonal variations in reproductive parameters ofOuled Djellal rams in the East of Algeria

Author(s):  
Yamina Belkhiri ◽  
Farida Bouzebda-Afri ◽  
Zoubir Bouzebda ◽  
Charef Eddine Mouffok ◽  
Amal Djaout

This experiment was carried out on Ouled Djellal rams in order to study the effect of season on testicular size, serum testosterone level and semen characteristics for a period of one year. Blood samples were obtained via jugular vein, and semen was collected by using an electro-ejaculator from six fertile male rams (2-4 years of age) on a monthly basis. In this breed, testis parameters varied seasonally showing the highest average values during autumn. However, the variation of body weight, GSI, serum testosterone levels, mass motility, individual motility and sperm concentration were not significant during the sampling period. The results reported show that the lowest and highest levels of semen volume were recorded in winter (0.62±0.11 ml) and in autumn (1.23±0.09 ml) respectively. The pH value and total number in sperm showed a uniform pattern with low scores during breeding season and high scores in non-breeding season. High percentage of live sperm was recorded during the summer, and high percentage of abnormal sperm was recorded during winter.

2014 ◽  
Vol 38 (1) ◽  
pp. 24-29
Author(s):  
Ali A. Abd

The aim of this study was to evaluate the effect of age and season on the epididymal sperm and level of testosterone in camel. A total 103 camel testes samples were collected from Al-Najaf slaughter house during a cold season (December 2012, January and February 2013) and moderatehot season (March, April and May, 2013). Testes were divided into 3 Gs according to camel age, G1 included the testes of 2-3years, G 2 (3 – 4years) and over 4 years (G 3). Blood samples were collected for determination of serum testosterone level. The sperms were obtained from the tail of epididymis from all animals groups and the results of the sperms individual motility percentage was increased at the level of (P< 0.05) significantly with age progress in both seasons. Also, sperm motility of G3 recorded a significantly higher than those of G1 and G2 in cold and moderate-hot seasons .The live percentage of G 3 animals was 90.01% in cold season with a significantly higher than those of other Gs and in both seasons. However, the live sperm percentage of G 3 during moderate-hot season was 87.82% and G 2 during moderate-hot and cold seasons were 88.58 and 88.72% respectively, showed significantly higher than those of G1 during cold and moderate-hot seasons .The concentration of sperm obtained from epididymis tail of bulls camel significantly increase with age progress in both seasons. However, the mean of the sperm concentration in cold season showed significance higher than those in moderate-hot season in animals of G 1 and 3, respectively. The abnormal morphologically sperm percentage of animals G 1 in both cold and moderate-hot seasons were significantly higher than those of G3.The testosterone levels of the young animals (under 4 years) increased gradually and reached its peak in February 2.28 ng/ml and March 2.27ng/ml. In the same trend older animal (more than 4 years) was showed 8.14 and 7.35 ng/ml, respectively. The older animals showed a significantly monthly, higher values than those of the younger animals in their testosterone level started from January up to May. In conclusions during cold months the camel over 4 years shows higher percentage of epididymal sperms parameters (live and individual motility) and sperms concentration as well as serum testosterone level.


2000 ◽  
Vol 71 (2) ◽  
pp. 317-324 ◽  
Author(s):  
T. A. Taha ◽  
E. I. Abdel-Gawad ◽  
M. A. Ayoub

AbstractFifteen sexually mature rams, five each of Barki, Awassi (I, imported from Syria) and Awassi (LB, locally born in Egypt) were used in this study. Semen was collected monthly from rams for a period of 12 months to study semen characteristics. In addition, blood samples were collected from rams during the four seasons of the year to determine serum triiodothyronine (T3) and testosterone hormones. Results showed that Barki and Awassi (I and LB) rams are continuous breeders as they show sexual desire and produce semen all the year round. However, monthly variations in semen quality were detected. Relative testes volume, ejaculate volume, sperm concentration, total sperm output, sperm motility, percentage of live sperm and serum testosterone level were higher during summer months than at other months of the year. Serum T3 was significantly higher in winter and spring than that observed in summer and autumn. In addition, percentages of dead spermatozoa were higher in winter and early spring than in autumn and summer. Furthermore, summer months showed moderate percentages of abnormal spermatozoa and spermatozoa without intact acrosomes. These results indicate that ram semen quality in the subtropics may be better during summer months than during other months of the year.


2009 ◽  
Vol 21 (9) ◽  
pp. 35
Author(s):  
A. Towhidi ◽  
A. Farshad ◽  
M. Dolatpanah ◽  
R. Salehi

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.


2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
L. Dinithi. C. Peiris ◽  
M. A. T. Dhanushka ◽  
T. A. H. D. G. Jayathilake

Treatment with 100 mg/kg and 200 mg/kg body weight of aqueous leaf extract (ALE) ofCardiospermum halicacabumfor 30 days produced a significant dose dependent increase in the sperm counts and sperm motility in both caput and cauda regions. Further, significant increase in serum testosterone level was evident at all applied doses. However, no significant changes in the weight of sex organs were observed. Aqueous leaf extract also increased the number of females impregnated, number of implantations, and number of viable fetuses while decreasing the total number of resorption sites in the pregnant females. However, the total cholesterol level in the serum remained unchanged and there were no records on renotoxicity; nevertheless ALE exhibited a hepatoprotective effect. It was concluded that aqueous leaf extract ofCardiospermum halicacabumenhanced sperm concentration, motility, and testosterone, leading to positive results in fertility.


2020 ◽  
Vol 15 (03) ◽  
pp. 45-48
Author(s):  
K. H. Parmar ◽  
FS Kavani ◽  
TK Patbandha ◽  
SS Parikh

The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43 and 93.56±0.56, respectively, whereas the mean post-thawed sperm characteristics, viz., progressive sperm motility, live sperm (%), abnormal sperm (%), HOS reactive sperm (%), acrosomal integrity (%) and first insemination conception rate (%) observed were 57.60±0.36, 66.34±0.53, 8.85±0.33, 56.97±0.46, 75.26±0.17 and 44.63±0.14, respectively. The semen quality of fresh and post-thawed samples observed was within normal limit for use in breeding program with satisfactory first insemination conception rate.


2013 ◽  
Vol 53 (6) ◽  
pp. 548 ◽  
Author(s):  
M. M. Ommati ◽  
M. J. Zamiri ◽  
A. Akhlaghi ◽  
H. Atashi ◽  
M. R. Jafarzadeh ◽  
...  

Seminal characteristics and blood biochemical attributes were studied in breeder roosters orally administered with sage extract (SG), an herbal extract well known to have potent antioxidant activities. Sixty roosters (34 weeks old) were randomly allotted to five treatment groups to receive no SG, or orally administered with 110, 210, 320, or 420 mg SG/kg liveweight for 8 weeks. Semen samples were evaluated weekly. Blood samples were taken fortnightly and a total of 21 biochemical indices were measured to unmask the effects of SG (especially the adverse ones) on the clinical profile. Excluding the sperm concentration and seminal content of thiobarbituric acid reactive substances (sperm membrane lipid peroxidation index), other seminal traits exhibited one of the linear, quadratic, or cubic responses to the various levels of SG. The most improvements in total live sperm number and sperm membrane integrity (as determined by the hypoosmotic swelling test) were observed in birds receiving 210 and 320 mg SG/kg liveweight, respectively. Serum testosterone level was generally higher (cubically, P = 0.015), but serum copper was lower (linearly, P = 0.014) in SG-administered birds. Birds receiving 320 and 420 mg SG showed a decreased content of C18 : 2(n-6) in sperm plasma membrane. Other biochemical attributes or sperm fatty acids were not affected. It seems that most improvement in the seminal characteristics could be achieved 5–6 weeks following the administration of 210 and 320 mg SG/kg liveweight without any apparent adverse effect on the blood biochemical indices. The improvements, however, could not be attributed to the antioxidative effect of SG. Although it is hypothesised that an increased serum testosterone might have been involved, the underlying mechanism(s) remain(s) to be clarified.


2021 ◽  
Vol 33 (2) ◽  
pp. 160
Author(s):  
S. Dholpuria ◽  
G. N. Purohit ◽  
S. Vyas

The present study aimed to evaluate the effects of exogenous administration of melatonin to male camels on reproductive behaviour, seminal parameters, and serum concentrations of melatonin and testosterone during the non-breeding and subsequent breeding seasons. In September 2018, male camels (n=12) were randomly allocated to subcutaneous injections of either melatonin (MLT, 0.643 mg/kg; n=6) dissolved in corn oil, or plain corn oil; (control n=6). The camels were observed for behavioural signs of rut. A second dose of either MLT (1 mg/kg) or corn oil (control) was administered on 7 October 2018 subcutaneously, as camels did not show any change in behaviour until 6 October 2018, and the camels were again observed for behavioural signs of rut. Blood was collected from all camels on 1 September 2018, and then at weekly intervals until 7 January 2019. The serum was separated and stored until further assay of serum melatonin and testosterone using ELISA kits. Semen was collected from camels showing signs of rut period, and the reaction time, copulation time, and seminal parameters were evaluated. Data obtained was analysed by using the computer program SPSS (version 25; IBM Corp.), and means and standard errors were compared. Male reproductive behaviours (protrusion of soft palate, poll gland secretions, frequent urination, increased frothy salivation) were more pronounced in MLT-treated camels (83.33%) than in control camels (50%). Reaction time and copulation times were lower (112.81±30.06s and 229.8±37.91s) in MLT-treated camels than in controls (359.00±41.35s and 301.89±93.90s). Higher semen volume was observed in MLT-treated (5.34±2.06mL) than in control (3.11±1.75mL) camels (P&lt;0.01). Semen collected from MLT-treated camels had more gel formation (81.25%), significantly higher sperm motility (67.50±2.32%), and increased sperm concentration (326.56±38.67 million mL−1) compared with samples obtained from the control males (P&lt;0.01). Serum melatonin concentrations increased in both treated and control camels during the breeding season compared with concentrations before the breeding season. In MLT-treated camels, serum melatonin concentrations increased significantly and continued to be significantly higher than that in control camels for the entire study period (P&lt;0.01). Serum testosterone concentrations were higher in both MLT-treated and control camels during the breeding season compared with those during the non-breeding season (P&lt;0.01). We concluded that exogenous subcutaneous MLT administration improves sexual behaviour, seminal parameters, and serum testosterone concentrations during both the non-breeding and breeding seasons.


Author(s):  
Meltem Mermer ◽  
Yasemin Akdevelioğlu

Obesity causes many health problems as well as having negative effects on fertility. There is a multifaceted relationship between obesity and male infertility. Male fertility is associated with sperm concentration, motility and morphology. Testosterone (total and free) and gonadotropin levels were low and estrogen levels were found to be high in obese men. The decrease in androgen levels varies based on the degree of obesity. High body mass index is inversely related to total sperm count, sperm concentration, sperm morphology and number of motile sperm. Leptin and ghrelin are hormones that influence body weight regulation and eating behaviors. Serum leptin level, which is high in obese infertile men, showed a significant positive correlation with abnormal sperm morphology, serum FSH, LH, prolactin level and showed a significant negative correlation with sperm concentration, sperm motility and serum testosterone level. Further research is needed on the effects of ghrelin levels on male infertility. The relationship between obesity and erectile dysfunction can be explained by decreased testosterone levels and elevation of some proinflammatory cytokine levels. Decreased physical activity due to obesity, long-term sitting increases testicular temperature and affects sperm production negatively. There is a multifaceted relationship between obesity, metabolic syndrome and type 2 diabetes and serum testosterone and SHBG. Diet and exercise showed improvement in testosterone levels and sperm concentration due to decreased body weight. However, studies on this subject are extremely limited. The possible effects of weight loss on successful infertility treatment of obese men should not be ignored.


2011 ◽  
Vol 29 (7_suppl) ◽  
pp. 159-159
Author(s):  
Y. Fujii ◽  
S. Yoshida ◽  
M. Yokoyama ◽  
Y. Iimura ◽  
N. Numao ◽  
...  

159 Background: Treatment with an LH-RH agonist is a standard alternative to surgical castration for prostate cancer patients. The serum testosterone level is kept at castrate levels continuously during LH-RH agonist therapy in almost all patients (Fujii Y, BJU Int 2008). LH- RH agonists, however, are more expensive than surgical castration, with drugs costing between US $300 and $500 per month in Japan. Recent studies suggest that 3-monthly formulations of LH-RH agonists suppress the serum testosterone levels far longer than the 3-month dosing interval. Methods: A total of 43 Japanese patients with prostate cancer who were treated with 3-monthly LH-RH agonists (23 with 11.25mg leuprolide, and 20 with 10.8 mg goserelin) for one year or longer and whose testosterone levels were kept at castrate level (defined as < 50 ng/dL) were entered into this prospective, longitudinal study. After entry, the 43 men received the same 3-monthly LH-RH agonists at 6-month intervals, and had serum LH and testosterone tests performed at 3-month intervals. Bicalutamide was combined with the LH-RH agonists in 12 of the patients. Results: At entry, median patient age was 74 years (range 59 to 89), median duration of LH-RH agonists treatment was 26 months (12 to 125), and median LH and testosterone levels were <10 ng/dL (<10 to 60) and 5 ng/dL (<5 to 18), respectively. The 43 patients received a total of 162 administrations (median 5, range 1 to 6) of the LH-RH agonists at 6-month intervals, and had a total 335 hormonal tests (median 10, range 2 to12) performed during the median followup period of 30 months. Serum LH and testosterone levels were kept suppressed during the treatment. Of the 43 patients, two had serum testosterone just above the castrate level (54 and 56 ng/dL) once each among their 12 and 8 hormonal assays, respectively. Conclusions: Administration of 3-monthly LH-RH agonists, either leuprolide or goserelin, at 6-month intervals could maintain the castrate level of serum testosterone at least in Japanese prostate cancer patients who have received LH-RH agonists treatment for one year or longer. No significant financial relationships to disclose.


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