scholarly journals Evaluation of Aqueous Leaf Extract ofCardiospermum halicacabum(L.) on Fertility of Male Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
L. Dinithi. C. Peiris ◽  
M. A. T. Dhanushka ◽  
T. A. H. D. G. Jayathilake
Keyword(s):  
Leaf Extract ◽  
Total Cholesterol Level ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Sperm Counts ◽  
Aqueous Leaf Extract ◽  
Dose Dependent Increase ◽  
Dose Dependent

Treatment with 100 mg/kg and 200 mg/kg body weight of aqueous leaf extract (ALE) ofCardiospermum halicacabumfor 30 days produced a significant dose dependent increase in the sperm counts and sperm motility in both caput and cauda regions. Further, significant increase in serum testosterone level was evident at all applied doses. However, no significant changes in the weight of sex organs were observed. Aqueous leaf extract also increased the number of females impregnated, number of implantations, and number of viable fetuses while decreasing the total number of resorption sites in the pregnant females. However, the total cholesterol level in the serum remained unchanged and there were no records on renotoxicity; nevertheless ALE exhibited a hepatoprotective effect. It was concluded that aqueous leaf extract ofCardiospermum halicacabumenhanced sperm concentration, motility, and testosterone, leading to positive results in fertility.

Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Toxicological Evaluation ◽  
Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

Increased Urinary Excretion of Carnitine and Acylcarnitine by Mercuric Chloride Is Reversed by 2,3-Dimercapto-1-Propanesulfonic Acid in Rats

2010 ◽  
Vol 29 (3) ◽  
pp. 313-317
Author(s):  
Waleed A. Al-Madani ◽  
Nikhat J. Siddiqi ◽  
Abdullah S. Alhomida ◽  
Haseeb A. Khan ◽  
Ibrahim A. Arif ◽  
...  
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Mercuric Chloride ◽  
Free Carnitine ◽  
Male Rats ◽  
Significant Protection ◽  
Total Carnitine ◽  
Dose Dependent Increase ◽  
Dose Dependent ◽  
Wistar Male Rats

This investigation was aimed to study the effect of 2,3-dimercapto-1-propanesulfonic acid (DMPS) on mercuric chloride (HgCl2)-induced alterations in urinary excretion of various carnitine fractions including free carnitine (FC), acylcarnitine (AC), and total carnitine (TC). Different groups of Wistar male rats were treated with HgCl2 at the doses of 0.1, 0.5, 1.0, 2.0, and 3.0 mg/kg body weight, and the animals were sacrificed at 24 hours following HgCl2 injection. A separate batch of animals received HgCl2 (2 mg/kg) with or without DMPS (100 mg/kg) and sacrificed at 24 or 48 hours after dosing. Administration of HgCl2 resulted in statistically significant and dose-dependent increase in the urinary excretion of FC, AC, and TC in rats. However, the ratio of urinary AC:FC was significantly decreased by HgCl2. Pretreatment with DMPS offered statistically significant protection against HgCl2-induced alterations in various urinary carnitine fractions in rats.

scholarly journals Antidiarrheal Activity of Dissotis multiflora (Sm) Triana (Melastomataceae) Leaf Extract in Wistar Rats and Subacute Toxicity Evaluation

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Alian Désiré Afagnigni ◽  
Maximilienne Ascension Nyegue ◽  
Chantal Florentine Ndoye Foe ◽  
Youchahou Njankouo Ndam ◽  
Frédéric Nico Njayou ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Female Rats ◽  
Male Rats ◽  
Dependent Manner ◽  
Subacute Toxicity ◽  
Antidiarrheal Activity ◽  
Dose Dependent ◽  
Ethanolic Leaf Extract

The present work was undertaken to evaluate antidiarrheal activity of ethanolic leaf extract of Dissotis multiflora (Sm) Triana (D. multiflora) on Shigella flexneri-induced diarrhea in Wistar rats and its subacute toxicity. Diarrhea was induced by oral administration of 1.2 × 109 cells/mL S. flexneri to rats. Antidiarrheal activity was investigated in rats with the doses of 111.42 mg/kg, 222.84 mg/kg, and 445.68 mg/kg. The level of biochemical parameters was assessed and organs histology examined by 14 days’ subacute toxicity. S. flexneri stool load decreased significantly in dose-dependent manner. The level of ALT increased (p<0.05) in male rats treated with the dose of 445.68 mg/kg while creatinine level increased in rats treated with both doses. In female rats, a significant decrease (p<0.05) of the level of AST and creatinine was noted in rats treated with the dose of 222.84 mg/kg of D. multiflora. Histological exams of kidney and liver of treated rats showed architectural modifications at the dose of 445.68 mg/kg. This finding suggests that D. multiflora leaf extract is efficient against diarrhea caused by S. flexneri but the treatment with doses lower than 222.84 mg/kg is recommended while further study is required to define the exact efficient nontoxic dose.

scholarly journals Effect of age and season on the Epididymal Sperm and testosterone level in camel (camelus dromedarius)

2014 ◽  
Vol 38 (1) ◽  
pp. 24-29
Author(s):  
Ali A. Abd
Keyword(s):  
Testosterone Level ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Cold Season ◽  
Serum Testosterone Level ◽  
Epididymal Sperm ◽  
Hot Season ◽  
Live Sperm ◽  
Effect Of Age

The aim of this study was to evaluate the effect of age and season on the epididymal sperm and level of testosterone in camel. A total 103 camel testes samples were collected from Al-Najaf slaughter house during a cold season (December 2012, January and February 2013) and moderatehot season (March, April and May, 2013). Testes were divided into 3 Gs according to camel age, G1 included the testes of 2-3years, G 2 (3 – 4years) and over 4 years (G 3). Blood samples were collected for determination of serum testosterone level. The sperms were obtained from the tail of epididymis from all animals groups and the results of the sperms individual motility percentage was increased at the level of (P< 0.05) significantly with age progress in both seasons. Also, sperm motility of G3 recorded a significantly higher than those of G1 and G2 in cold and moderate-hot seasons .The live percentage of G 3 animals was 90.01% in cold season with a significantly higher than those of other Gs and in both seasons. However, the live sperm percentage of G 3 during moderate-hot season was 87.82% and G 2 during moderate-hot and cold seasons were 88.58 and 88.72% respectively, showed significantly higher than those of G1 during cold and moderate-hot seasons .The concentration of sperm obtained from epididymis tail of bulls camel significantly increase with age progress in both seasons. However, the mean of the sperm concentration in cold season showed significance higher than those in moderate-hot season in animals of G 1 and 3, respectively. The abnormal morphologically sperm percentage of animals G 1 in both cold and moderate-hot seasons were significantly higher than those of G3.The testosterone levels of the young animals (under 4 years) increased gradually and reached its peak in February 2.28 ng/ml and March 2.27ng/ml. In the same trend older animal (more than 4 years) was showed 8.14 and 7.35 ng/ml, respectively. The older animals showed a significantly monthly, higher values than those of the younger animals in their testosterone level started from January up to May. In conclusions during cold months the camel over 4 years shows higher percentage of epididymal sperms parameters (live and individual motility) and sperms concentration as well as serum testosterone level.

scholarly journals Effect of aqueous extract of Date Palm Pollen (DPP) on the sperm characteristic and Serum Testosterone, FSH and LH Values in albino male rats treated with sodium floride

2014 ◽  
Vol 38 (2) ◽  
pp. 41-47
Author(s):  
Salah M. M. AL-Chalabi
Keyword(s):  
Aqueous Extract ◽  
Date Palm ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Male Rats ◽  
Maximum Effect ◽  
Control Group ◽  
Abnormal Sperm ◽  
Group 2 ◽  
Date Palm Pollen

     The present study was undertaken to evaluate the effect of aqueous extract of Date Palm Pollen DPP on the testicular function and serum testosterone, FSH and LH hormones value. Thirty five male rats were divided randomly into five equal groups. Group 1: received 0.5 ml of distilled water (control group), group 2: was treated orally 0.250 p.p.m of sodium florid (NaF) (with volume of 0.5 ml / rat), Group 3: was treated with 0.250 p.p.m of NaF and 50 mg/kg. B.W. of DPP extract (0.5ml D.W \rat), Group 4: was treated with 0.250 p.p.m of NaF and 100 mg/kg. B.W. of DPP extract and Group 5: was treated with 0.250 p.p.m of NaF and 150 mg/kg. B.W. of DPP extract. The results showed  significant (P< 0.05) decrease in sperm concentration, motility and significant (P< 0.05) increases in dead and abnormal sperm in the group 2 in comparison to control, while all groups of  DPP extract showed significant (P< 0.05) increase in  sperm concentration, motility and decrease in dead and abnormal sperm. Maximum effect was observed in animals treated with a dose of 150 mg/kg of DPP extract, also the results revealed significant (P< 0.05) increase in testosterone, FSH and LH hormones in groups treated with DDP in comparison to G1andG2. Male rats received DPP for 50 days showed significant (P< 0.05) increases in body and testes weight as compared to G1andG2. In conclusion the results revealed that the aqueous extract of DPP pollen can be used as a sex enhancer and seems to cure male infertility.

scholarly journals Toxic Effects of Trazodone on Male Reproductive System via Disrupting Hypothalamic-Pituitary-Testicular Axis and Inducing Testicular Oxidative Stress

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Sinem Ilgın ◽  
Gözde Aydoğan-Kılıç ◽  
Merve Baysal ◽  
Volkan Kılıç ◽  
Mina Ardıç ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Reproductive Toxicity ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Toxic Effects ◽  
Male Rats ◽  
Hormonal Changes ◽  
Depression And Anxiety ◽  
Sperm Dna Damage

Depression and anxiety are recognized as public health problems. Epidemiological studies have shown that depression and anxiety often occur during reproductive ages between 20 and 60 years of age in males. Trazodone is one of the most frequently prescribed drugs in the treatment of depression and anxiety. Drugs used in repeated doses also play a role in the etiology of infertility. In our study, it was aimed to identify the possible toxic effects of trazodone on male rats and elucidate the underlying mechanisms. Vehicle or trazodone (5, 10, and 20 mg/kg/day) was administered to rats for 28 consecutive days (n=8 per group). At the end of that period, sperm concentration, motility, morphology, and DNA damage were determined and testicular morphology was assessed histopathologically in rats. Additionally, we investigated hormonal status by determining serum testosterone, FSH, and LH levels and oxidative stress by determining glutathione and malondialdehyde levels in testicular tissue to elucidate mechanisms of possible reproductive toxicity. According to our results, sperm concentration, sperm motility, and normal sperm morphology were decreased; sperm DNA damage was increased in trazodone-administered groups. Degenerative findings on the testicular structure were observed after trazodone administration in rats. Additionally, serum FSH, LH, and testosterone levels were elevated in the trazodone-administered groups. Increased MDA levels were the signs of enhanced oxidative stress after trazodone administration in testis tissues. Thus, we concluded that trazodone induced reproductive toxicity in male rats; this reproductive toxicity was accompanied by oxidative stress and hormonal changes, which are considered as important causes of reproductive disorders.

scholarly journals Semen quality in patients with pituitary disease and adult-onset hypogonadotropic hypogonadism

2018 ◽  
Vol 7 (4) ◽  
pp. 523-533 ◽  
Author(s):  
Mikkel Andreassen ◽  
Anders Juul ◽  
Ulla Feldt-Rasmussen ◽  
Niels Jørgensen
Keyword(s):  
Semen Quality ◽  
Hypogonadotropic Hypogonadism ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Free Testosterone ◽  
Pituitary Insufficiency ◽  
Adult Onset ◽  
Pituitary Disease ◽  
Sperm Counts ◽  
The Individual

Objective Gonadotropins (luteinizing hormone (LH) and follicle-stimulating hormone (FSH)) are released from the pituitary gland and stimulate Leydig cells to produce testosterone and initiates spermatogenesis. Little is known about how and when the deterioration of semen quality occurs in patients with adult-onset gonadotropin insufficiency. Design and methods A retrospective study comprising 20 testosterone-deficient men (median age, 29 years) with acquired pituitary disease who delivered semen for cryopreservation before initiation of testosterone therapy. Semen variables and hormone concentrations were compared to those of young healthy men (n = 340). Results Thirteen of 20 patients (65%) and 82% of controls had total sperm counts above 39 million and progressive motile spermatozoa above 32% (P = 0.05). For the individual semen variables, there were no significant differences in semen volume (median (intraquartile range) 3.0 (1.3–6.8) vs 3.2 (2.3–4.3) mL, P = 0.47), sperm concentration 41 (11–71) vs 43 (22–73) mill/mL (P = 0.56) or total sperm counts (P = 0.66). One patient had azoospermia. Patients vs controls had lower serum testosterone 5.4 (2.2–7.6) vs 19.7 (15.5–24.5) nmol/L (P = 0.001), calculated free testosterone (cfT) 145 (56–183) vs 464 (359–574) pmol/L (P < 0.001), LH 1.5 (1.1–2.1) vs 3.1 (2.3–4.0) U/L (P = 0.002) and inhibin b (P < 0.001). Levels of FSH were similar (P = 0.63). Testosterone/LH ratio and cfT/LH ratio were reduced in patients (both P < 0.001). Conclusions Despite Leydig cell insufficiency in patients with acquired pituitary insufficiency, the majority presented with normal semen quality based on the determination of the number of progressively motile spermatozoa. In addition, the data suggest reduced LH bioactivity in patients with pituitary insufficiency.

Seasonal variations in reproductive parameters ofOuled Djellal rams in the East of Algeria

2017 ◽  
Author(s):  
Yamina Belkhiri ◽  
Farida Bouzebda-Afri ◽  
Zoubir Bouzebda ◽  
Charef Eddine Mouffok ◽  
Amal Djaout
Keyword(s):  
Breeding Season ◽  
Ph Value ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Sampling Period ◽  
Serum Testosterone Level ◽  
Monthly Basis ◽  
Testicular Size ◽  
Live Sperm ◽  
One Year

This experiment was carried out on Ouled Djellal rams in order to study the effect of season on testicular size, serum testosterone level and semen characteristics for a period of one year. Blood samples were obtained via jugular vein, and semen was collected by using an electro-ejaculator from six fertile male rams (2-4 years of age) on a monthly basis. In this breed, testis parameters varied seasonally showing the highest average values during autumn. However, the variation of body weight, GSI, serum testosterone levels, mass motility, individual motility and sperm concentration were not significant during the sampling period. The results reported show that the lowest and highest levels of semen volume were recorded in winter (0.62±0.11 ml) and in autumn (1.23±0.09 ml) respectively. The pH value and total number in sperm showed a uniform pattern with low scores during breeding season and high scores in non-breeding season. High percentage of live sperm was recorded during the summer, and high percentage of abnormal sperm was recorded during winter.

Sign in / Sign up

Export Citation Format

Share Document