Growth of lentil (Lens culinaris Medikus) as influenced by phosphorus, Rhizobium and plant growth promoting rhizobacteria

Author(s):  
Guriqbal Singh ◽  
Narinder Singh ◽  
Veena Khanna

The experiment was conducted to study the effect of four levels of phosphorus (0, 20, 30 and 40 kg P2O5 ha-1) and four biofertilizer treatments [uninoculated control, Rhizobium, plant growth promoting rhizobacteria (PGPR) and Rhizobium + PGPR] on growth and grain yield of lentil. The experiment was conducted in factorial randomized complete block design (RCBD) with three replications. The periodic data recorded at 30, 60, 90, 120 days after sowing (DAS) and at harvest showed that the highest growth in various parameters i.e. plant height, branches plant-1 and shoot dry matter accumulation was recorded with application of 40 kg P2O5 ha-1, however, it was at par with 30 P2O5 ha-1. Among the biofertilizers, Rhizobium + PGPR treatment gave maximum values of growth parameters like plant height, branches plant-1 and shoot dry matter accumulation at all the stages. At 30-60 DAS, the maximum crop growth rate (CGR) was recorded with the application of 40 kg P2O5 ha-1 (71.3 kg ha-1 day-1) and co-inoculation of Rhizobium and PGPR (72.0 kg ha-1 day-1). Application of 40 P2O5 ha-1 and use of coinoculation (Rhizobium + PGPR) provided the highest grain yields. The study highlights the importance of phosphorus application and biofertilizers inoculation for improving the growth and grain yield of lentil.

2017 ◽  
Vol 9 (3) ◽  
pp. 1422-1428 ◽  
Author(s):  
Zorawar Singh ◽  
Guriqbal Singh ◽  
Navneet Aggarwal

The field experiment was conducted during 2015-16 to study the effect of biofetilizer inoculation [control, Mesorhizobium only, Mesorhizobium + RB-1 (Pseudomonas argentinensis) and Mesorhizobium + RB-2 (Bacillus aryabhattai)] and four levels of phosphorus (0, 15, 20 and 25 kg P2O5 ha-1) on chickpea growth. RB-1 and RB-2 were the plant growth promoting rhizobacteria (PGPR). Biofertilizers could play a crucial role in reducing the dependence on chemical fertilizers by fixing the atmospheric nitrogen for crop and/or by increasing the availability of phosphorus and phytohormones to the crop. The 16 treatment combinations were laid out in Factorial Randomized Complete Block Design and replicated three times. In biofertilizer treatments, Mesorhizobium + RB-1 proved superior over control and sole inoculation of Mesorhizobium and at par with Mesorhizobium + RB-2 with respect to plant height (cm), number of branches (plant-1), shoot and root dry matter (kg ha-1) which were recorded at 30, 60 90, 120 days after sowing (DAS) and at harvest. Application of 25 kg P2O5 ha-1 gave the highest values of all the growth at-tributes viz. plant height (60 cm), number of primary (5.3) and secondary (27.2) branches per plant, shoot dry matter (4000 kg ha-1) and root dry matter (354 kg ha-1) which were significantly higher than that of 0 and 15 kg P2O5 ha-1 and at par with 20 kg P2O5 ha-1. Similar results were observed in case of crop growth rate (CGR) whereas relative growth rate (RGR) was not influenced significantly by various biofertilizer and phosphorus treatments. The dual inoc-ulation with PGPR strains along with phosphorus application have a supplementary effect on the growth of chickpea.


2021 ◽  
Vol 10 (1) ◽  
pp. 1-5
Author(s):  
Muhammad Yusril Hardiansyah ◽  
Yunus Musa ◽  
Abdul Mollah Jaya

The low productivity of cocoa plantations in Indonesia is partly due to the low quality of seeds, which refers to the impeded growth of cultivated cocoa nurseries. Seed is the initial growth of plants so the importance of giving special treatment to seeds will refer to better seed growth. Provision of Plant Growth Promoting Rhizobacteria (PGPR) microbes can produce indoleacetic acid (IAA) in plants to improve the quality of plant growth. This study aims to determine the effectiveness of the provision of Plant Growth Promoting Rhizobacteria bamboo rhizosphere against cocoa seed germination. The study was carried out in the farmer group garden, Gantarangkeke District, Bantaeng. This study was arranged in the form of a two-factor factorial design (F2F) in a randomized block design (RBD). The use of cocoa seed type as the first factor consisted of GTB (Gantarangkeke Bantaeng) local cocoa seed and MCC 01 cocoa seed and seed immersion treatment at PGPR rhizosphere bamboo concentration as the second factor consisting of 0% (control) concentration, 5%, 10 % and 15%. The results obtained indicate that administration of seeds with bamboo rhizosphere PGPR affects the germination (100.00%), the speed of seed growth (7.14%/etmal), as well as on abnormal seeds (10.00%). So that the provision of bamboo rhizosphere PGPR on cocoa seeds has an effective influence on seed germination and cocoa seedling development.


2021 ◽  
Vol 21 (No 1) ◽  
Author(s):  
Aghajan bahadori ◽  
Mohmmad Hossein GHarineh ◽  
Abdolmahdi Bakhshandeh ◽  
Naeimeh Enayatizamir ◽  
Alireza Shafeinia

This study was performed in order to investigate the effect of Plant growth-promoting rhizobacteria in reducing nitrogen and phosphorus Fertilizers Application in Sugarcane. The field experiment of this study was in the form of Split–block design with subplots in stips with four replications and three factors, including bacterial factor at four levels (control, Enterobacter cloaca, Pseudomonas putida and a combination of two types of bacteria), nitrogen factor at three levels (50, 75 and 100% recommended nitrogen for sugarcane (and phosphorus factor at three levels (50, 75 and 100% recommended phosphorus for sugarcane), was carried out in 2016-2017crop year in DC7-10 research farm of Dehkhoda sugarcane agro-industryin Ahvaz, in the southwest of Iran, on CP73-21 sugarcane variety. According to the analysis of variance tables, simple and interaction effects of the tested treatments, in the case of quantitative traits, including stalk yield, height, diameter, stalk density, percentage of nitrogen and phosphorus of leaves, chlorophyll content, LAI and HI in sugarcane were significant at the level of 1% probability. Comparison of means showed that the application of simultaneous application of growth-promoting bacteria along with the application of 75% recommended nitrogen and phosphorus for sugarcane, compared with the control treatment (application of 100% recommended nitrogen and phosphorus for sugarcane, without the use of bacteria), Was able to succeed in these traits 96.9%, 98.1%, 95.7%, 96.3%, 100.2% ,101.9%, 91.2% and 94.8%, respectively and Provide 21/9, 23/1, 20/7, 21/3, 25, 25, 16.2 and 19.8% of the nutrients of nitrogen and phosphorus for sugarcane, respectively, and is saved the same amount of nitrogen and phosphorus consumption for sugarcane. Also, regarding the sugarcane yield, the simultaneous application treatment of the tested bacteria along with the application of 100% recommended phosphorus and nitrogen for sugarcane, Compared to the control treatment


2019 ◽  
Vol 7 (2) ◽  
pp. 64
Author(s):  
Syafrullah Salman

This research aims to find out effect of combination dosage of PGPR (Plant Growth Promoting Rhizobacteria) and phonska fertilizer toward the growth and the yield of soybean. This experiment was carried out in the field using a combination randomized block design (RBD) with five repeating.The treatments gave were 100% PGPR fertilizer (8 grams / liter) (A), 100% PGPR fertilizer (8 grams / liter) + 50% phonska fertilizer (0.78 grams) (B), 50% PGPR fertilizer (4 grams / liter) + 100% phonska fertilizer (1.56 gram) (C), 50% PGPR fertilizer (4 gram / liter) + 50% phonska fertilizer (0.78 gram) (D) and 100% phonska fertilizer (1, 56 grams) (E).The results showed that the treatment of 50% PGPR fertilizer (4 grams / liter) + phonska fertilizer 100% (1.56 grams) (C) gave a significant effect on the variable root length and leaf area index (LAI).The treatment of 100% phonska fertilizer (1.56 grams) (E) gave a significant effect on the variable seed weight per plot.


Akta Agrosia ◽  
2020 ◽  
Vol 23 (1) ◽  
pp. 33-37
Author(s):  
Fera Ariska ◽  
Marlin Marlin ◽  
Widodo Widodo

Bawang dayak is the important medicinal plant that need to be developed in cultivation techniques and production. The use of Plant Growth Promoting Rhizobacteria (PGPR) recently known to be effective to increase plant growth and yield. The research aimed to determine the optimal concentration and immersion time of PGPR on the growth and yield of bawang dayak. The experiment was arrange in complete randomized block design (RCBD) consisting of two factors. The first factor is the concentration of PGPR with 4 levels namely K0 = 0 g L-1, K1 = 5 g L-1, K2 = 10 g L-1 and K3 = 15 g L-1.  The second factor is immersion time of seed, namely P1 = 10 minutes, P2 = 20 minutes, P3 = 30 minutes and P4 = 40 minutes.  The results showed that there was an interaction between concentration and immersion time of PGPR giving effect to the number of leaves and the number of tillers. The immersion time of PGPR for 10 minutes with a concentration of 15 g L-1produced the highest number of leaves (58 leaves) and produced the highest number of tillers (27.67 tillers).  The treatment of PGPR concentration or immersion time of PGPR singly did not affect all observed variables of growth and yield of bawang dayak.


2020 ◽  
Vol 8 (1) ◽  
pp. 342
Author(s):  
Monyck Jeane dos Santos Lopes ◽  
Moacyr Bernardino Dias-Filho ◽  
Thomaz Henrique dos Reis Castro ◽  
Edilson Ferreira da Silva ◽  
Marcela Cristiane Ferreira Rêgo ◽  
...  

The aim of this study was to evaluate the effects of plant growth-promoting rhizobacteria effects on anatomical characteristics and nutritional value of Brachiaria (Syn. Urochloa) brizantha cv. BRS Piatã. The experimental design applied was completely randomized design  with three treatments: (1) non-inoculated unfertilized-control plants (C-), (2) non-inoculated fertilized-control plants (C+) and (3) B. brizantha inoculated with Pseudomonas fluorescens (BRM-32111) and Burkholderia pyrrocinia (BRM-32113). The following parameters were evaluated at 35 days after seedling emergence: biomass production, plant height, net photosynthesis (A), water-use efficiency (WUE), chlorophyll (SPAD), anatomical and nutritional. The rhizobacteria modified the anatomy of the leaf, culm and roots of B. brizantha. They also increased the chlorophyll content, A, WUE, total soluble carbohydrates, starch and crude protein contents, N, P, Mg and Fe concentrations, plant height, root area and biomass production. Therefore, we conclude that co-inoculation with P. fluorescens (BRM-32111) and B. pyrrocinia (BRM-32113) modified the anatomy and biochemistry of B. brizantha, promoting growth and nutrient accumulation. Therefore, these findings set up the basis for additional exploratory studies, using these rhizobacteria as biotechnological innovation with potential of use as biofertilizer in B. brizantha, aiming higher productivity and nutritive value in a more eco-friendly and sustainable pasture production system.


2001 ◽  
Vol 47 (6) ◽  
pp. 590-593 ◽  
Author(s):  
Abdelaly Hilali ◽  
Danielle Prévost ◽  
William J Broughton ◽  
Hani Antoun

One hundred strains of Rhizobium leguminosarum bv. trifolii were isolated from roots of wheat cultivated in rotation with clover in two different regions of Morocco. The isolates were first screened for their effect on the growth of the cultivar Rihane of wheat cultivated in an agricultural soil under greenhouse conditions. After 5 weeks of growth, 14 strains stimulating the fresh or dry matter yield of shoots were selected and used in a second pot inoculation trial performed with two different agricultural soils. The results show that the strains behaved differently according to the soil used. In the loamy sand Rabat, strain IAT 168 behaved potentially like a plant growth promoting rhizobacteria (PGPR), as indicated by the 24% increases (P < 0.1) observed in wheat shoot dry matter and grain yields. In the silty clay Merchouch, no PGPR activity was observed, and 6 strains showed a significant deleterious effect on yields. These observations suggest that it is very important in a crop rotation system to choose a R. leguminosarum bv. trifolii strain that is effective with clover and shows PGPR activity with wheat to avoid deleterious effects on wheat yields.Key words: deleterious bacteria, PGPR (plant growth promoting rhizobacteria), Trifolium alexandrinum, Triticum aestivum.


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