scholarly journals Automation of Seed Priming Technology for Enhanced Seed Vigour of Blackgram Seeds

Author(s):  
K. Malarkodi ◽  
T. Vedhapriya ◽  
R. Umarani ◽  
M. Bhaskaran

Background: Seed priming technique involves soaking of seeds in priming agent to a point where germination related metabolic activities occur in the seeds followed by drying of imbibed seeds to original moisture to prevent the radical protrusion and facilitate storage of primed seed. Draining of priming agent and drying of large quantity of primed seeds is a laborious and time consuming process. The current study was aimed to standardize automation of seed priming process by utilizing house-hold top loading washing machine. Methods: The top loading washing machine referred to as Seed Priming Cabinet, contained a priming drum with provision for draining water. On completion of the soaking period, the ‘SPIN’ button was pressed by setting the duration of spin drying (1, 2, 3, 4 and 5 min.). Then the seeds were removed from the drum of priming cabinet and subjected to shade drying so as to reach the original moisture content and evaluated for seed quality parameters. Result: The blackgram seed subjected to conventional hydropriming was compared with automatic seed priming for 1, 2, 3, 4 and 5 min. and observed that hydropriming and spin drying for 3 min. was found to be optimum and the per cent increase in germination recorded over control was 18.0 per cent thus suggesting that automation of seed priming technology can be recommended for enhanced seed vigour and crop productivity of blackgram.

Author(s):  
Prashant Kumar Rai Kotamarthi Bhavana ◽  
G. M. Lal

The current experiment was conducted to evaluate the Performance of Organic amendments and botanical treatments on seed quality parameters of French bean under stimulated environmental conditions. An experiment was conducted in 2021 at postgraduate seed testing laboratory, Department of Genetics and Plant Breeding Naini Agriculture Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj. The French bean Cultivar Arka Bold seeds were used for priming. Organic treatments are designated as Panchagavya 2%, 4%,6%,8%, Agniasthra 2%,4%,6%,8% and Botanicals treatments are Tulasi leaf extract 5%, Aloevera leaf extract 5%, Vinca rosea leaf extract 5% and also did hydroprming treatment using distilled water respectively. Seeds were soaked in organic, botanicals and in distilled water for duration of 6 hours followed by shade drying. The results were obtained and it was found that all the treatments showed significant effect on quality parameters. The highest was found in panchgavya of all doses followed by tulasi leaf extract found to be best in Germination percentage, Speed of Germination, Root length, Shoot length, Seedling length, Seed vigour index-I, Seed vigour Index-II compared to control or untreated seeds which indicates the effect of organics and botanicals on physiological processes of the seeds. This study revealed that priming with organic amendments and botanicals can be effective in enhancing the seed quality parameters of french bean and they are low cost, effective and nature friendly and toxic free.


Author(s):  
K. Yashwanth Chandra ◽  
Arun Kumar Chaurasia

The present research study was conducted at the Seed testing laboratory of Sam Higginbottom Institute of Agriculture Technology and Sciences, Prayagraj, Uttar Pradesh in 2020-2021. The research study revealedthat effect of seed priming treatments with Panchagavya, Jeevamrutha, Beejamrutha and leaf extracts, Sodium molybdate on seed quality parameters of field pea was analyzed through Completely Randomized Design (CRD). Organic treatments are designated as Panchagavya 3% and 5%, Jeevamrutha 3% and 5%, Beejamrutha 5% are used as treatment 8Hrs respectively, botanical treatments are Tulasi leaf extract 6%, Lantana camara leaf extract for 6% are used as treatment for duration of 4 hours respectivelyin which the treatment T8- Sodium molybdate (3%) for 3 Hrs exhibited higher mean value for seed germination (76.75%), root length (6.49 cm), shoot length (7.84 cm), seedling length (14.33 cm), seed vigour index -I (1099.68) and Followed by value was exhibited by T6-Tulasi Leaf Extract (6%) with respect of Root length (6.79 cm)Seedling dry weight (1.725 g), Seed Vigour Index II (129.78), T0 (control) with respect of Germination percentage (71.75%) root length (6.30 cm), shoot length (6.60 cm) and seedling length (12.90 cm), seed vigour index I &II (925.60 & 98.13).


Author(s):  
S. Kavitha ◽  
P. Srimathi

Background: Black gram is the important pulses crop, grown throughout the country; gives low seed yield mainly due to poor management and low soil fertility. Development of suitable technology is essential to enhance productivity. Seed priming is one such effective technology which enhance rapid and uniform emergence to achieve high vigour, leading to better stand establishment and yield. The current research aimed to study micronutrient seed priming and rhizobium coating on seed germination, seedling vigour, crop growth and seed yield in black gram.Methods: Blackgram seeds were primed with 10 different micronutrient solutions with different concentrations and evaluated for its quality parameters to find out suitable seed priming technique. Seeds from two best priming treatments were coated with rhizobium @ 30g/kg of seed and evaluated for its production potential and resultant seed quality under field condition.Result: Seed priming with 0.5% ZnSO4 at 1/3rd seed to solution ratio for 3 hours recorded higher germination (96%) and vigour index (3811) than control which recorded 84% seed germination and 2528 of vigour index under laboratory experiment. Field experiments revealed that, seeds primed with 0.5% ZnSO4 and coated with rhizobium @ 30g per kg of seeds improved seed yield by 13.2% over control. 


Author(s):  
C. Vanitha ◽  
M. Kathiravan

Background: Seed priming is controlled hydration and dehydration that results in improved seed quality and yield. However, hydration-dehydration of fresh seeds would result in soaking injury especially in legumes. The disadvantage reported in pulses can overcome through pre-conditioning of seeds which allows the seeds to slow hydration and prevent the soaking injury. The conditioning of seeds in large scale is tedious and skill oriented. To overcome this problem, an innovative seed priming technology is essential for pulses to enhance the seed germination, vigour and seed yield. Methods: Experiment was carried out at National Pulses Research Centre, Vamban during 2016-18. Seed priming technology was developed by using marginal vigour lot of blackgram. The marginal vigour seeds were packed in gunny bag up to 3/4th level and soaked in water for 2 h and 4 h and incubated for 2 h, 4 h and 6 h. After the incubation period, seeds were dried back to original moisture content (9.0%) and seed quality parameters were analyzed. The best performing organic priming concentrations under laboratory were forwarded to field trial and yield parameters were assessed. Result: Marginal vigour lot of blackgram seeds packed in gunny bag and soaked in seaweed extract @ 0.4% for 2 h and incubated for 4 h significantly increased the seed quality parameters. Among the various organic priming, marginal vigour lot of blackgram seeds packed in gunny bag and soaked in seaweed extract @ 0.4% for 2 h and incubated for 4 h significantly increased the seed quality parameters and yield over hydro priming and control.


Author(s):  
T. N. Tiwari ◽  
D. K. Agarwal

Field experiments were conducted with four levels of seed priming including control and two varieties of pigeon pea at ICAR-Indian Institute of Seed Science, Mau during three consecutive years (2011-12 to 2013-14). One-year-old seeds of pigeon pea varieties (Bahar and Malviya-13) were primed with growth regulator (100 ppm GA3), in-organic salt (0.2% KNO3) and tap water (sanitized) separately for 06 hrs. and sown in field under RBD with 03 replications. Observations were recorded on seed quality parameters, biochemical attributes including nitrate assimilatory enzymes and activities of anti-oxidant enzymes during seedling stage. Seed quality parameters including germination, seedling growth and vigor indices were significantly enhanced through seed priming with GA3 followed by KNO3 and tap water over unprimed control. Biochemical attributes viz; chlorophyll a andb contents, were more influenced with GA3 priming followed by KNO3 and tap water whereas the proline accumulation was reduced with priming treatments and maximum reduction was noted with GA3 followed by KNO3 and tap water. Enhancement in nitrate assimilatory enzymes including nitrate and nitrite reductase activities was more with KNO3 priming followed by GA3 and tap water. Anti-oxidant enzymes activities including Catalase, Peroxidase and Super Oxide Dismutase were also increased significantly by KNO3 priming followed by GA3 and tap water over unprimed control.


2019 ◽  
Vol 46 (6) ◽  
pp. 584
Author(s):  
Lilya Boucelha ◽  
Réda Djebbar ◽  
Ouzna Abrous-Belbachir

Pre-germination treatments represent the physiological methods that improve plant production by modulating the metabolic activities of germination before the emergence of the radicle. It was suggested that reactive oxygen species (ROS) play a crucial role in signalling seed germination. Our work consisted in studying changes in the redox status in the embryonic axis (radicle and plumule) and in cotyledons of Vigna unguiculata (L.) Walp. non-primed, osmoprimed (30% PEG6000), hydroprimed or twice hydroprimed seeds, by estimating antioxidant activities and production of ROS. Some antioxidant enzymatic activities as well as total non-enzymatic antioxidant capacity were measured. The production of hydrogen peroxide (H2O2) and superoxide anion (O2–) was also assessed by 3,3′-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) respectively. The results obtained showed, on the one hand, that priming allows activation of antioxidant enzymes, especially in the plumule. On the other hand, these results showed that priming caused an accumulation of ROS in embryonic tissues. This may explain the improvement of seed germination performance according to the oxidative window model. Priming induced changes in the redox environment at the seed level. These changes were closely related to the pre-germination treatments. Indeed, a double cycle of hydration-rehydration induced the broadest spectrum of modifications of the redox status, which would explain the improvement of the seed vigour.


Author(s):  
S . Poonguzhali

Studies were undertaken at the Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore using different seed lots of blackgram cv. CO 6 with a view to elicit information on seed invigouration for maintenance of seed quality during storage period. Seeds soaking followed by drying for different durations ranging from 1h, 2h and 3h with different inorganic chemicals viz., Vitamin E (a- tocopherol), Para Amino Benzoic Acid (PABA) and Salicylic Acid at concentrations of 2.5 mM, 5.0 mM and 7.5 mM in 1:1 seed to solution ratio was attempted. The results shows that soaking of varying seed lots of freshly harvested, aged treated and treated aged seeds of blackgram (3 days of accelerated ageing) in low concentrations viz., 2.5 mM, 5.0 mM of salicylic acid followed by drying was effective in controlling seed deterioration and improving seed vigour and viability in all the seed lots.


2015 ◽  
Vol 7 (3) ◽  
pp. 316-321
Author(s):  
Seyed Ata SIADAT ◽  
Seyed Amir MOOSAVI ◽  
Mehran SHARAFIZADEH

Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing) and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm) and different duration of treatment (8, 12 and 24 hour). Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.


2021 ◽  
Vol 911 (1) ◽  
pp. 012086
Author(s):  
Ramlah Arief ◽  
Fauziah Koes ◽  
Oom Komalasari ◽  
Fatmawati

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.


Author(s):  
Kranti M. Pawar ◽  
Prashant Kumar Rai

This present experiment was conducted in a Seed Testing laboratory, Department of Genetics and Plant Breeding SHUATS, Allahabad, UP during 2021 with bottle gourd, in order to standardize the best method of invigoration of priming specific to bottle gourd. method of priming viz., halopriming were evaluated by screening a different range of durations and chemical concentrations viz., T0 – Unprimed seeds (control), T₁ - 50 ppm KNO3 (Potassium Nitrate) for 12 hrs, T₂ - 50 ppm KNO3 for 18 hrs, T₃-100 ppm KNO3 for 12 hrs, T₄- 100 ppm KNO3 for 18 hrs, T₅-125 ppm KNO3 for 12 hrs, T₆- 125 ppm KNO3 for 18 hrs. Treated seeds were placed in between germination paper and in sand at control condition where data was subjected to factorial experiment laid out in completely randomised design (CRD). In which it was found that 6th treatment 125 ppm KNO3 for 18 hrs., has the significant result as compared to all the treatments with the control, the highest germination %, seedling length, weight and vigor index I & II. This study showed that Seed priming with KNO3 found to increase the seed quality parameters. In the present study proved market price- effective and most economical method. This study helps to improve the quality and performance of seeds with the help of seed priming treatments which have effect on market price and economic, non-toxic, eco-friendly sources.


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