Improving vigour and viability of blackgram cv.co 6 [Vigna mungo (L) Hepper] through seed priming with inorganics

2016 ◽  
Author(s):  
S . Poonguzhali
Keyword(s):  
Salicylic Acid ◽  
Tamil Nadu ◽  
Seed Quality ◽  
Storage Period ◽  
Seed Priming ◽  
Accelerated Ageing ◽  
Seed Vigour ◽  
Seed Deterioration ◽  
Low Concentrations ◽  
Amino Benzoic Acid

Studies were undertaken at the Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore using different seed lots of blackgram cv. CO 6 with a view to elicit information on seed invigouration for maintenance of seed quality during storage period. Seeds soaking followed by drying for different durations ranging from 1h, 2h and 3h with different inorganic chemicals viz., Vitamin E (a- tocopherol), Para Amino Benzoic Acid (PABA) and Salicylic Acid at concentrations of 2.5 mM, 5.0 mM and 7.5 mM in 1:1 seed to solution ratio was attempted. The results shows that soaking of varying seed lots of freshly harvested, aged treated and treated aged seeds of blackgram (3 days of accelerated ageing) in low concentrations viz., 2.5 mM, 5.0 mM of salicylic acid followed by drying was effective in controlling seed deterioration and improving seed vigour and viability in all the seed lots.

Biochemical Investigations on Vigour Enhancement in Fresh and Aged Seeds Upon Seed Priming in Cowpea [Vigna unguiculata (L.) Walp.]

2021 ◽  
Author(s):  
M.N. Arun ◽  
K. Bhanuprakash ◽  
S. Shankara Hebbar ◽  
T. Senthivel ◽  
A.K. Nair ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Seed Quality ◽  
Protein Profile ◽  
Seed Priming ◽  
Ammonium Molybdate ◽  
Cellular Damage ◽  
Repair System ◽  
Seed Vigour ◽  
Seed Deterioration ◽  
Aged Seeds

Background: Loss in seed quality that occurs from maturity in the field to storage, leads to seed deterioration. Storage of cowpea seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzyme and protein levels. Method: The present study was performed to verify the effects of cowpea [Vigna unguiculata (L.) Walp.] seed priming (GA3, ammonium molybdate, Ca Cl2, KBr, Mg (NO3)2, ZnSO4, Hydro priming and dry non primed) with fresh and accelerated aged seeds.Result: The deterioration was rectified to the extent possible by the technique of seed priming. SDS PAGE profiling indicated the differential expression of proteins with seed priming. Esterase and peroxidase enzyme which were completely lost as a result of ageing showed reappearance after priming. The band intensity as well as the number of proteins induced by seed priming increased over control. Priming also restored the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds. The present study concluded that priming with GA3 (100 ppm) and Ammonium Molybdate (10-3 M) for 24 hours in aged seeds of cowpea showed increase enzyme activity, restored almost entire protein profile and esterase and peroxidise isozyme profile as it allowed repair system to combat sub-cellular damage and activated synthesis of enzymes and proteins.

scholarly journals Enhancement of Tainan 9 Peanut Seed Storability and Germination under Low Temperature

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Parinee Jeammuangpuk ◽  
Parichart Promchote ◽  
Juangjun Duangpatra ◽  
Tanapon Chaisan ◽  
Damrongvudhi Onwimol ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Low Temperature ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Storage Period ◽  
Seed Priming ◽  
Seed Vigor ◽  
Peanut Seed ◽  
Temperature Conditions ◽  
Before And After

Low temperature condition during December to January can limit seed emergence and seedling establishment for peanut production in Thailand. The objective of this study was to determine the effects of peanut seed priming on seed germination and vigor under optimal and low temperature conditions before and after 9 months of storage. Tainan 9 peanut seeds were primed with salicylic acid (SA), ascorbate (ASA), CaCl2, or chitosan and tested for germination at 25°C (optimal temperature) and 15°C (low temperature) before and after a 9-month storage period. Seed priming with 50 mg·L−1 SA and 50 mg·L−1 ASA for 12 hours before germinating improved germination at 15°C when compared to untreated seeds both before and after 9-month storage. The high seed quality, illustrated by high germination percentage, high seed vigor, and low mean germination time related to the low autoxidation substrates: lipoxygenase (LOX), malondialdehyde (MDA), and high antioxidants: superoxide dismutase (SOD) and catalase (CAT). It suggests that peanut seed priming with salicylic acid and/or ascorbate can improve seedling emergence and growth under low temperature conditions.

scholarly journals Biochemical investigations on vigour enhancement in aged seeds upon seed priming in onion

2016 ◽  
Vol 8 (2) ◽  
pp. 855-859 ◽  
Author(s):  
Umesha Umesha ◽  
S.N. Vasudevan ◽  
K. Bhanuprakash ◽  
B. Manjunatha ◽  
G. Sarika ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Seed Quality ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigour ◽  
Protein Levels ◽  
Seed Deterioration ◽  
Metabolic Defects ◽  
Induced Proteins ◽  
Aged Seeds

Loss in seed quality that occurs during all process, from maturation in the field to storage, leads to seed deterioration. Storage of onion seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzymes and protein levels. This deterioration can be rectified to the extent possible by the technique of seed priming. The results clearly indicated that priming will restore the lost seed vigour in aged seeds due to reactivation of enzyme activity in old seeds. The germination percentage improved from 60% to 79.5% and 72.5% in GA3 and hydroprimed, respectively. Priming will also restore the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds.

Effect of foliar application of growth activator, promoterand antioxidant on seed quality of soybean

2016 ◽  
Author(s):  
P . Kuchlan ◽  
M. K. Kuchlan ◽  
S. M. Husain
Keyword(s):  
Salicylic Acid ◽  
Seed Yield ◽  
Seed Quality ◽  
Foliar Application ◽  
Foliar Spray ◽  
Soybean Seed ◽  
Seed Vigour ◽  
Filling Stage ◽  
Seed Health

Loss of seed quality in soybean is a serious problem in tropical and subtropical region. Foliar application of salicylic acid (SA), GA3 and acetyl salicylic acid (50 ppm) at seedling and pod filling stage, K2HPO4 (2%), a-tocopherol (100 ppm) at flowering and pod filling stage was done on soybean varieties – NRC 7 and JS 335. Foliar application of SA showed positive effect on seed yield, seed health, germination and seed vigour during storage. Plant height was significantly improved by the GA3 spray in both the varieties. Significantly higher seed yield was obtained with foliar spray of a-tocopherol. Application of SA was found effective to protect plants from various diseases to get quality seed. a-Tocopherol and K2HPO4 treatment significantly reduced MDA production in seeds. a-Tocopherol application increased super oxide dismutase activity as a result the lipid peroxidation of seed during storage was significantly reduced. Application of a-tocopherol, K2HPO4 and salicylic acid improved the storage or keeping quality of soybean seed.

scholarly journals Impact of Organic Seed Priming Amendments and Botanicals on Seed Quality Parameters of French Bean (Phaseolous vulgaris L.)

2021 ◽  
Vol 10 (11) ◽  
pp. 259-265
Author(s):  
Prashant Kumar Rai Kotamarthi Bhavana ◽  
G. M. Lal
Keyword(s):  
Leaf Extract ◽  
Seed Quality ◽  
Low Cost ◽  
Organic Amendments ◽  
Seed Priming ◽  
Quality Parameters ◽  
French Bean ◽  
Distilled Water ◽  
Seed Vigour ◽  
Vigour Index

The current experiment was conducted to evaluate the Performance of Organic amendments and botanical treatments on seed quality parameters of French bean under stimulated environmental conditions. An experiment was conducted in 2021 at postgraduate seed testing laboratory, Department of Genetics and Plant Breeding Naini Agriculture Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj. The French bean Cultivar Arka Bold seeds were used for priming. Organic treatments are designated as Panchagavya 2%, 4%,6%,8%, Agniasthra 2%,4%,6%,8% and Botanicals treatments are Tulasi leaf extract 5%, Aloevera leaf extract 5%, Vinca rosea leaf extract 5% and also did hydroprming treatment using distilled water respectively. Seeds were soaked in organic, botanicals and in distilled water for duration of 6 hours followed by shade drying. The results were obtained and it was found that all the treatments showed significant effect on quality parameters. The highest was found in panchgavya of all doses followed by tulasi leaf extract found to be best in Germination percentage, Speed of Germination, Root length, Shoot length, Seedling length, Seed vigour index-I, Seed vigour Index-II compared to control or untreated seeds which indicates the effect of organics and botanicals on physiological processes of the seeds. This study revealed that priming with organic amendments and botanicals can be effective in enhancing the seed quality parameters of french bean and they are low cost, effective and nature friendly and toxic free.

scholarly journals Effect of Seed Priming Treatments with Panchagavya, Jeevamrutha, Beejamrutha and Leaf Extracts, Sodium Molybdate on Seed Quality Parameters of Field Pea (Pisum sativum)

2021 ◽  
pp. 24-33
Author(s):  
K. Yashwanth Chandra ◽  
Arun Kumar Chaurasia
Keyword(s):  
Root Length ◽  
Leaf Extract ◽  
Seed Quality ◽  
Sodium Molybdate ◽  
Seed Priming ◽  
Quality Parameters ◽  
Leaf Extracts ◽  
Seed Vigour ◽  
Seedling Length ◽  
Vigour Index

The present research study was conducted at the Seed testing laboratory of Sam Higginbottom Institute of Agriculture Technology and Sciences, Prayagraj, Uttar Pradesh in 2020-2021. The research study revealedthat effect of seed priming treatments with Panchagavya, Jeevamrutha, Beejamrutha and leaf extracts, Sodium molybdate on seed quality parameters of field pea was analyzed through Completely Randomized Design (CRD). Organic treatments are designated as Panchagavya 3% and 5%, Jeevamrutha 3% and 5%, Beejamrutha 5% are used as treatment 8Hrs respectively, botanical treatments are Tulasi leaf extract 6%, Lantana camara leaf extract for 6% are used as treatment for duration of 4 hours respectivelyin which the treatment T8- Sodium molybdate (3%) for 3 Hrs exhibited higher mean value for seed germination (76.75%), root length (6.49 cm), shoot length (7.84 cm), seedling length (14.33 cm), seed vigour index -I (1099.68) and Followed by value was exhibited by T6-Tulasi Leaf Extract (6%) with respect of Root length (6.79 cm)Seedling dry weight (1.725 g), Seed Vigour Index II (129.78), T0 (control) with respect of Germination percentage (71.75%) root length (6.30 cm), shoot length (6.60 cm) and seedling length (12.90 cm), seed vigour index I &II (925.60 & 98.13).

scholarly journals Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

2015 ◽  
Vol 7 (3) ◽  
pp. 316-321
Author(s):  
Seyed Ata SIADAT ◽  
Seyed Amir MOOSAVI ◽  
Mehran SHARAFIZADEH
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Morphological Structure ◽  
Seed Priming ◽  
Silybum Marianum ◽  
Duration Of Treatment ◽  
Seed Vigour ◽  
Germination Characteristics ◽  
Poor Seed ◽  
Seed Ageing

Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing) and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm) and different duration of treatment (8, 12 and 24 hour). Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

Assessment of variation in seed longevity within rye, wheat and the intergeneric hybrid triticale

2009 ◽  
Vol 19 (4) ◽  
pp. 213-224 ◽  
Author(s):  
Maciej Niedzielski ◽  
Christina Walters ◽  
Wieslav Luczak ◽  
Lisa M. Hill ◽  
Lana J. Wheeler ◽  
...  
Keyword(s):  
Shelf Life ◽  
Seed Quality ◽  
Seed Longevity ◽  
Accelerated Ageing ◽  
Species Variation ◽  
Seed Vigour ◽  
Intergeneric Cross ◽  
Dry Storage ◽  
Dry Conditions ◽  
Initial Seed

AbstractSeed shelf-life or longevity is difficult to predict or to measure on a practical time scale. Predictive models suggest that water has the same effect on ageing rate for all seed lots within a species and that initial seed quality is the dominating factor explaining within-species variation. These assumptions are used in ‘accelerated ageing’ or ‘controlled deterioration’ (AA/CD) tests, which are commonly used to predict seed longevity in commercial and research applications. In this study, we describe within-species variation of longevity for seeds of Secale cereale and S. strictum (cultivated rye and its wild progenitor) under typical dry storage conditions of a genebank, and show that initial seed quality is an important, but not sole, factor explaining measured longevity. We also test the correlation of seed longevity, measured under humid and dry conditions, using 50 cultivars of rye, wheat (Triticum aestivum) and the intergeneric cross triticale, to assess how well AA/CD tests predict seed shelf-life during dry storage. Known differences in longevity between wheat and rye were confirmed at all water contents, and triticale seeds demonstrated intermediate behaviour. Longevity measured for humid and dry conditions were weakly correlated when comparisons included all grain types and were not correlated in within-grain-type comparisons. Response to moisture varied among cultivars. These findings do not support assumptions made in seed ageing models that use AA/CD tests. Our results suggest that more traits are involved in the expression of seed longevity than those typically measured in studies of initial seed vigour.

scholarly journals Influence of Different Maturity Stages on Tomato (Solanum lycopersicum L.) Seed Quality during Storage at -20°C

2021 ◽  
Author(s):  
Rashied Tetteh ◽  
Lawrence Misa Aboagye ◽  
Robert Darko
Keyword(s):  
Seed Quality ◽  
Storage Period ◽  
Freezing Temperature ◽  
Germination Percentage ◽  
Vegetable Crops ◽  
Seed Vigour ◽  
Maturity Stages ◽  
Solanum Lycopersicum L ◽  
Tomato Seeds

Background: Tomato is one of the most important vegetable crops in the world due to its dietary value. Harvesting and storing of physiologically matured tomato seeds is very important to secure good quality seeds for future use in a changing climate. The objective of the study was to assess the effect of maturity stages on seed quality of two tomato accessions during cold storage. Methods: Seeds of two tomato accessions extracted at four maturity stages (i.e. initially ripe, half ripe, fully ripe and rotten) were stored at -20oC in a deep freezer for 12 months. The quality of stored seeds was measured by seed vigour and germination percentage at 3, 6, 9 and 12 months after storage. Conclusion: The study showed significant differences in seed vigour and germination percentage at 3, 6, 9 and 12 months after storage (MAS) among the maturity stages. Seeds extracted at the initially ripe stage in GH 9305 had the least vigour at 3, 6, 9 and 12 MAS. The highest reduction in seed quality during the storage period was observed in seeds extracted at the rotten stage in both accessions. Seed vigour and germination were higher in GH 9207 than GH 9305. Storage of physiologically matured tomato seeds at freezing temperature is necessary to reduce seed ageing and to maintain high seed quality.

scholarly journals Automation of Seed Priming Technology for Enhanced Seed Vigour of Blackgram Seeds

2021 ◽  
Author(s):  
K. Malarkodi ◽  
T. Vedhapriya ◽  
R. Umarani ◽  
M. Bhaskaran
Keyword(s):  
Seed Quality ◽  
Seed Priming ◽  
Crop Productivity ◽  
Quality Parameters ◽  
Washing Machine ◽  
Seed Vigour ◽  
Original Moisture ◽  
Cent Increase ◽  
Priming Process ◽  
Metabolic Activities

Background: Seed priming technique involves soaking of seeds in priming agent to a point where germination related metabolic activities occur in the seeds followed by drying of imbibed seeds to original moisture to prevent the radical protrusion and facilitate storage of primed seed. Draining of priming agent and drying of large quantity of primed seeds is a laborious and time consuming process. The current study was aimed to standardize automation of seed priming process by utilizing house-hold top loading washing machine. Methods: The top loading washing machine referred to as Seed Priming Cabinet, contained a priming drum with provision for draining water. On completion of the soaking period, the ‘SPIN’ button was pressed by setting the duration of spin drying (1, 2, 3, 4 and 5 min.). Then the seeds were removed from the drum of priming cabinet and subjected to shade drying so as to reach the original moisture content and evaluated for seed quality parameters. Result: The blackgram seed subjected to conventional hydropriming was compared with automatic seed priming for 1, 2, 3, 4 and 5 min. and observed that hydropriming and spin drying for 3 min. was found to be optimum and the per cent increase in germination recorded over control was 18.0 per cent thus suggesting that automation of seed priming technology can be recommended for enhanced seed vigour and crop productivity of blackgram.

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