Vigna unguiculata seed priming is related to redox status of plumule, radicle and cotyledons

Pre-germination treatments represent the physiological methods that improve plant production by modulating the metabolic activities of germination before the emergence of the radicle. It was suggested that reactive oxygen species (ROS) play a crucial role in signalling seed germination. Our work consisted in studying changes in the redox status in the embryonic axis (radicle and plumule) and in cotyledons of Vigna unguiculata (L.) Walp. non-primed, osmoprimed (30% PEG6000), hydroprimed or twice hydroprimed seeds, by estimating antioxidant activities and production of ROS. Some antioxidant enzymatic activities as well as total non-enzymatic antioxidant capacity were measured. The production of hydrogen peroxide (H2O2) and superoxide anion (O2–) was also assessed by 3,3′-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) respectively. The results obtained showed, on the one hand, that priming allows activation of antioxidant enzymes, especially in the plumule. On the other hand, these results showed that priming caused an accumulation of ROS in embryonic tissues. This may explain the improvement of seed germination performance according to the oxidative window model. Priming induced changes in the redox environment at the seed level. These changes were closely related to the pre-germination treatments. Indeed, a double cycle of hydration-rehydration induced the broadest spectrum of modifications of the redox status, which would explain the improvement of the seed vigour.

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Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

Notulae Scientia Biologicae ◽

10.15835/nsb739528 ◽

2015 ◽

Vol 7 (3) ◽

pp. 316-321

Author(s):

Seyed Ata SIADAT ◽

Seyed Amir MOOSAVI ◽

Mehran SHARAFIZADEH

Keyword(s):

Seed Germination ◽

Seed Quality ◽

Morphological Structure ◽

Seed Priming ◽

Silybum Marianum ◽

Duration Of Treatment ◽

Seed Vigour ◽

Germination Characteristics ◽

Poor Seed ◽

Seed Ageing

Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing) and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm) and different duration of treatment (8, 12 and 24 hour). Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

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Automation of Seed Priming Technology for Enhanced Seed Vigour of Blackgram Seeds

Legume Research - An International Journal ◽

10.18805/lr-4662 ◽

2021 ◽

Author(s):

K. Malarkodi ◽

T. Vedhapriya ◽

R. Umarani ◽

M. Bhaskaran

Keyword(s):

Seed Quality ◽

Seed Priming ◽

Crop Productivity ◽

Quality Parameters ◽

Washing Machine ◽

Seed Vigour ◽

Original Moisture ◽

Cent Increase ◽

Priming Process ◽

Metabolic Activities

Background: Seed priming technique involves soaking of seeds in priming agent to a point where germination related metabolic activities occur in the seeds followed by drying of imbibed seeds to original moisture to prevent the radical protrusion and facilitate storage of primed seed. Draining of priming agent and drying of large quantity of primed seeds is a laborious and time consuming process. The current study was aimed to standardize automation of seed priming process by utilizing house-hold top loading washing machine. Methods: The top loading washing machine referred to as Seed Priming Cabinet, contained a priming drum with provision for draining water. On completion of the soaking period, the ‘SPIN’ button was pressed by setting the duration of spin drying (1, 2, 3, 4 and 5 min.). Then the seeds were removed from the drum of priming cabinet and subjected to shade drying so as to reach the original moisture content and evaluated for seed quality parameters. Result: The blackgram seed subjected to conventional hydropriming was compared with automatic seed priming for 1, 2, 3, 4 and 5 min. and observed that hydropriming and spin drying for 3 min. was found to be optimum and the per cent increase in germination recorded over control was 18.0 per cent thus suggesting that automation of seed priming technology can be recommended for enhanced seed vigour and crop productivity of blackgram.

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Micro-nutrients Seed Priming and rhizobium Coating on Seed Vigour, Crop Growth and Seed Yield in Blackgram (Vigna mungo L.)

Legume Research - An International Journal ◽

10.18805/lr-4488 ◽

2021 ◽

Author(s):

S. Kavitha ◽

P. Srimathi

Keyword(s):

Seed Germination ◽

Seed Yield ◽

Field Experiments ◽

Seed Quality ◽

Seed Priming ◽

Quality Parameters ◽

Crop Growth ◽

Seedling Vigour ◽

Seed Vigour ◽

Vigour Index

Background: Black gram is the important pulses crop, grown throughout the country; gives low seed yield mainly due to poor management and low soil fertility. Development of suitable technology is essential to enhance productivity. Seed priming is one such effective technology which enhance rapid and uniform emergence to achieve high vigour, leading to better stand establishment and yield. The current research aimed to study micronutrient seed priming and rhizobium coating on seed germination, seedling vigour, crop growth and seed yield in black gram.Methods: Blackgram seeds were primed with 10 different micronutrient solutions with different concentrations and evaluated for its quality parameters to find out suitable seed priming technique. Seeds from two best priming treatments were coated with rhizobium @ 30g/kg of seed and evaluated for its production potential and resultant seed quality under field condition.Result: Seed priming with 0.5% ZnSO4 at 1/3rd seed to solution ratio for 3 hours recorded higher germination (96%) and vigour index (3811) than control which recorded 84% seed germination and 2528 of vigour index under laboratory experiment. Field experiments revealed that, seeds primed with 0.5% ZnSO4 and coated with rhizobium @ 30g per kg of seeds improved seed yield by 13.2% over control.

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Biochemical Investigations on Vigour Enhancement in Fresh and Aged Seeds Upon Seed Priming in Cowpea [Vigna unguiculata (L.) Walp.]

Legume Research - An International Journal ◽

10.18805/lr-4476 ◽

2021 ◽

Author(s):

M.N. Arun ◽

K. Bhanuprakash ◽

S. Shankara Hebbar ◽

T. Senthivel ◽

A.K. Nair ◽

...

Keyword(s):

Vigna Unguiculata ◽

Seed Quality ◽

Protein Profile ◽

Seed Priming ◽

Ammonium Molybdate ◽

Cellular Damage ◽

Repair System ◽

Seed Vigour ◽

Seed Deterioration ◽

Aged Seeds

Background: Loss in seed quality that occurs from maturity in the field to storage, leads to seed deterioration. Storage of cowpea seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzyme and protein levels. Method: The present study was performed to verify the effects of cowpea [Vigna unguiculata (L.) Walp.] seed priming (GA3, ammonium molybdate, Ca Cl2, KBr, Mg (NO3)2, ZnSO4, Hydro priming and dry non primed) with fresh and accelerated aged seeds.Result: The deterioration was rectified to the extent possible by the technique of seed priming. SDS PAGE profiling indicated the differential expression of proteins with seed priming. Esterase and peroxidase enzyme which were completely lost as a result of ageing showed reappearance after priming. The band intensity as well as the number of proteins induced by seed priming increased over control. Priming also restored the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds. The present study concluded that priming with GA3 (100 ppm) and Ammonium Molybdate (10-3 M) for 24 hours in aged seeds of cowpea showed increase enzyme activity, restored almost entire protein profile and esterase and peroxidise isozyme profile as it allowed repair system to combat sub-cellular damage and activated synthesis of enzymes and proteins.

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Combined seed enhancement technique involving seed priming and coating for improvised anatomical potential and vigour of okra seeds (Abelmoschus esculentus L.)

Journal of Phytology ◽

10.25081/jp.2019.v11.3857 ◽

2019 ◽

pp. 25-31

Author(s):

Anilkumar L ◽

Malarkodi K

Keyword(s):

Seed Germination ◽

Seed Priming ◽

Germination Percentage ◽

Abelmoschus Esculentus ◽

Seed Coating ◽

Seed Vigour ◽

Root Sheath ◽

Matter Production ◽

Seedling Length ◽

Vigour Index

The study was conducted to standardize the methodology of humid priming for improving the seed germination and vigour of okra seeds and to compare the efficacy of different enhancement techniques such as hydro priming, spin priming, seed coating and the combination of ‘humid priming + seed coating’ in improving the seed vigour characteristics of okra (Abelmoschus esculentus L.). In order to standardize the humid priming technique, okra seeds were subjected to humid priming for four different duration’s viz., 2, 4, 6 and 8 h, shade dried to original moisture content and subjected to evaluation of seed germination under shade net conditions in raised beds. The results revealed that 4 h of humid priming was statistically significant to the rest of the durations, with respect seed germination and seedling growth. With respect to anatomical potential, the radicle length (3.39mm), cotyledon length (6.69mm) and cotyledon width (6.30mm), recorded by humid priming (4 h) was 62.2 %, 6.4 % and 2.6 % higher than control seeds. The second experiment conducted to compare the efficacy of hydro priming, spin priming, polymer coating and combination of humid priming + seed coating. The study revealed that seeds subjected to combination of humid priming (4 h) + seed coating (3g kg-1) recorded highest physiological potential in terms of germination percentage (87 %), seedling length (20.2 cm), dry matter production (421.3 mg), vigour index (1765), root volume (3.5 cm3) and root sheath (2.560 g) while the control recorded only 70 %, 15.1 cm, 328.7 mg, 1055, 2.0 cm3 and 1.532 g, respectively.

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SEED PRIMING: A MULTIFACETED AND COST-EFFECTIVE TECHNIQUE TO IMPROVE CROP PRODUCTION

Kongunadu Research Journal ◽

10.26524/krj256 ◽

2018 ◽

Vol 5 (1) ◽

pp. 64-68

Author(s):

Sabarni Biswas ◽

Alivia Paul ◽

Asok Kumar Biswas

Keyword(s):

Abiotic Stress ◽

Seed Germination ◽

Antioxidant Enzyme ◽

Crop Production ◽

Membrane Damage ◽

Cost Effective ◽

Seed Priming ◽

Effective Technique ◽

Seed Vigour ◽

Cost Effective Technique

Seed priming is a cost-effective technique which involves prior seed exposure to an abiotic stress that makes the seed more resistant to future lethal exposure. Seed priming stimulates pre-germination metabolic processes and makes the seed ready for sprouting. It helps to up regulate the antioxidant enzyme activitiesand repairs membrane damage. These changes promote seed vigour and emergence under abiotic stress. This article aims to review the different priming processes as well as the physiological, biochemical and molecular changes induced by priming that lead to synchronized seed germination. Plants’ responses to some priming agents under abiotic stress have been reported based on recent investigations.

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Effect of Seed Priming with Proline on Germination and Seedling Growth of Mungbean under Salt Stress

South Asian Journal of Agriculture ◽

10.3329/saja.v7i1-2.56744 ◽

2019 ◽

pp. 15-18

Author(s):

Muhammad Abdus Sobahan

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Seedling Growth ◽

Seed Priming ◽

Germination Percentage ◽

Fresh Weight ◽

Seed Vigour ◽

Control Seed ◽

Vigour Index ◽

Radicle Length

Seed priming is a pre-sowing treatment that enhances germination performance and stress tolerance of germinating seeds. The effect of seed priming with proline on germination and seedling growth of mungbean (Vigna radiata L.) under salt stress was investigated. The experiment carried out in completely randomized design with three replications in May 2018 at the Research Laboratory of the School of Agriculture and Rural Development, Bangladesh Open University, Gazipur, Bangladesh. Salt stress at 5 dSm-1 decreased seed germination percentage, plumule length, radicle length, plumule fresh weight, radicle fresh weight and seed vigour index compared to control. Seed priming with proline increased germination percentage (53.84%), plumule length, radicle length, plumule fresh weight, radicle fresh weight and seed vigour index under salt stress. The results suggested that seed priming with proline could effectively alleviate the inhibitory effects of salt stress on seed germination and seedling growth of mungbean. South Asian J. Agric., 7(1&2): 15-18

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Alleviating Salinity Stress During Seed Germinating by Priming Techniques : A Review

International Journal of Scientific Research in Science and Technology ◽

10.32628/ijsrst218225 ◽

2021 ◽

pp. 198-202

Author(s):

Sandhya R Verma ◽

Hitesh A Solanki

Keyword(s):

Abiotic Stress ◽

Seed Germination ◽

Antioxidant Defense ◽

Seed Priming ◽

Antioxidant Defense System ◽

Germination Percentage ◽

Adverse Conditions ◽

Radicle Protrusion ◽

Priming Process ◽

Metabolic Activities

Seed germination and seedling growth are the two critical stages for crop establishment. These stages are the most sensitive to abiotic stresses, which decreases the germination percentage and increases germination time. Due to such abiotic stress, the germination of crop fails in adverse conditions. Salinity is one of the major abiotic stress, which adversely affects almost every aspect of the plant’s physiology, biochemistry and decreases yield. Salinity is the most severe threat to agriculture and major environmental factors that limit crop growth and productivity. Various techniques have been shown to improve emergence and stand establishment under salt stress. One of the most frequently utilized technique is seed priming. The seed priming process deals with the prior exposure of abiotic stress, making a seed more resistant to future exposure. Seed priming stimulates pre-germination metabolic activities and enhances radicle protrusion. It enhances the antioxidant defense system and the repair of membranes. The process of seed priming and the mechanism of the effect of salinity on seed germination have been discussed. The physiological, biochemical, and molecular changes induced by priming leading to seed enhancement have also been covered.

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Gibberellic Acid Biosynthesis During Dehydration Phase of Priming Increases Seed Vigour of Tomato

10.21203/rs.3.rs-522160/v1 ◽

2021 ◽

Author(s):

Seethalakshmi S ◽

Umarani R ◽

Djanaguiraman Maduraimuthu

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Seed Priming ◽

Seedling Vigour ◽

Seed Vigour ◽

Seedling Traits ◽

Physiological Processes ◽

Germination Process ◽

Priming Process ◽

Subsequent Effect

Abstract Priming of seed is intended to reduce the time to germination through activation of pre-germinative processes. Seed priming is controlled hydration and followed by a drying (dehydration) process. The physiological processes during hydration (imbibition) were studied in detail in tomato. In contrast, gibberellic acid (GA) changes during the dehydration phase were not studied in detail. We hypothesize that there would be a change in the GA concentration during the dehydration phase of the seed priming process, which may influence the vigour characteristics of the resultant seedling. The objective was to understand the influence dehydration phase of seed priming on GA biosynthesis and its subsequent effect on seed germination and seedling traits of tomato. First, the hydroprimed and unprimed seeds were re-imbibed for 3, 6, 9, and 12 h to initiate the germination process, and the GA concentration and seedling vigour associated parameters were recorded. In the second experiment, the imbibed seeds were dehydrated for 3, 6, 9, and 12 h, then re-imbibed for 3, 6, 9, and 12 h to understand the effect of dehydration on the GA concentration and its associated traits. Results revealed that hydroprimed seeds had a higher GA concentration and seedling vigour than unprimed seeds. The seeds that are completely dehydrated for 12 h had the highest GA and seed vigour parameters. Therefore, increased vigour of hydroprimed seeds is due to the higher levels of GA accumulated during the dehydration phase of seed priming, which can improve seed germination and seedling vigour of tomato.

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Seed Priming: An Interlinking Technology between Seeds, Seed Germination and Seedling Establishment

10.5772/intechopen.100804 ◽

2021 ◽

Author(s):

Sananda Mondal ◽

Bandana Bose

Keyword(s):

Seed Germination ◽

Seedling Establishment ◽

Seed Priming ◽

Growth Cycle ◽

Complex Processes ◽

Seed Physiology ◽

Seed Reserves ◽

Adverse Environmental Conditions ◽

Metabolic Activities ◽

Critical Phases

Biologically seed is a small embryonic plant along with either endosperm or cotyledons, enclosed with in an outer protecting covering called seed coat. During the time of seed development large metabolic conversions take place, including proper partitioning of photo-assimilates and the formation of complex polymeric forms of carbohydrate, protein and fats for storing as seed reserves. In developing phase of seeds, every detail information stored in the embryonic plant are genetically and sometimes epigenetically also predetermined and influenced by various environmental/external factors already faced by the mother plant. In the growth cycle of plants, seed germination and seedling establishment are the two critical phases where survivability of the seedlings in natural habitats is a matter of question until the onset of photosynthesis by the established seedling. The various sequence of complex processes known to occur in both the phases i.e., an array of metabolic activities are initiating which eventually leads to the renewal of embryo growth of the dormant seeds and ultimately seedlings are established. Efficient seed germination is an important factor for agricultural sciences and successful establishment of germinated seedling requires a rapid and uniform emergence and root growth. With these aspects of seed physiology kept in mind the present chapter will be designed in such a way where, a gap filling, inter linking, eco- and farmers\' friendly technology i.e., ‘seed priming’ (a pre-sowing partial hydration of seeds) will be considered to improve the rate and uniformity of germination and seedling establishment. Under optimal and adverse environmental conditions, the primed seeds of diversified species lead to an enhanced germination performance with increased vigor index has been reported by various scientists which indicates a good establishment of seedlings in the field and thereafter enhance the performance of crops as a whole.

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