Selection by Genetic Expression Profiles of Desi and Kabuli Chickpea (Cicer arietinum L.) Genotypes Tolerant to High Temperature Stress

2020 ◽  
Author(s):  
A. P. Rodríguez-Vera ◽  
J. A. Acosta-Gallegos ◽  
J. E. Ruiz-Nieto ◽  
V. Montero-Tavera
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Expression Profiles ◽  
Grain Filling ◽  
High Temperature Stress ◽  
Soil Conditions ◽  
Tolerance Index ◽  
Genetic Characteristics ◽  
Induced Genes ◽  
Shock Proteins

Background: Mexico is an important producer of chickpea; however, high temperatures during flowering and grain filling limit seed yield and seed size. Plant adaptation strategies to heat stress depend on climatic and soil conditions, but mainly on the plant genetic characteristics. The increase in heat shock proteins (HSP) production occurs when plants experience an abrupt or gradual increase in temperature in order to whithstand stress with the least damage. Methods: Sixty-five Heat Shock Protein related genes that induce transcription under heat stress were studied according to their expression profiles. This strategy allows for the selection of chickpea genotypes bearing potential heat stress tolerance. Based on the number of overexpressed (induced) genes and on its level of expression, a tolerance index was calculated. Result: Tolerant desi genotypes were: ICC 10259, ICC 13020, ICC 4958 and Annigeri; and in the kabuli type outstanding genotypes were: Mazocahui, ICCV 2, Blanco Sinaloa 92, Tequi Blanco 95, Combo 743 and CUGA 08-1210. These genotypes showed profiles with a higher number of induced genes and higher Tolerance Indexes. These genotypes will be further evaluated in the field and under controlled conditions and in the near future used as parental stocks.

scholarly journals Proteomic analysis of heat shock proteins in maize (Zea mays L.)

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Mahmoud Hussien Abou-Deif ◽  
Mohamed Abdel-Salam Rashed ◽  
Kamal Mohamed Khalil ◽  
Fatma El-Sayed Mahmoud
Keyword(s):  
Heat Treatment ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Proteome Analysis ◽  
High Temperature Stress ◽  
Heat Treatments ◽  
Maize Plants ◽  
Adverse Influence ◽  
Shock Proteins

Abstract Background Maize is one of the important cereal food crops in the world. High temperature stress causes adverse influence on plant growth. When plants are exposed to high temperatures, they produce heat shock proteins (HSPs), which may impart a generalized role in tolerance to heat stress. Proteome analysis was performed in plant to assess the changes in protein types and their expression levels under abiotic stress. The purpose of the study is to explore which proteins are involved in the response of the maize plant to heat shock treatment. Results We investigated the responses of abundant proteins of maize leaves, in an Egyptian inbred line of maize “K1”, upon heat stress through two-dimensional electrophoresis (2-DE) on samples of maize leaf proteome. 2-DE technique was used to recognize heat-responsive protein spots using Coomassie Brilliant Blue (CBB) and silver staining. In 2-D analysis of proteins from plants treated at 45 °C for 2 h, the results manifested 59 protein spots (4.3%) which were reproducibly detected as new spots where did not present in the control. In 2D for treated plants for 4 h, 104 protein spots (7.7%) were expressed only under heat stress. Quantification of spot intensities derived from heat treatment showed that twenty protein spots revealed clear differences between the control and the two heat treatments. Nine spots appeared with more intensity after heat treatments than the control, while four spots appeared only after heat treatments. Five spots were clearly induced after heat treatment either at 2 h or 4 h and were chosen for more analysis by LC-MSMS. They were identified as ATPase beta subunit, HSP26, HSP16.9, and unknown HSP/Chaperonin. Conclusion The results revealed that the expressive level of the four heat shock proteins that were detected in this study plays important roles to avoid heat stress in maize plants.

scholarly journals Insights Into Heat Response Mechanisms in Clematis Species: Physiological Analysis, Expression Profiles and Function Verification

2021 ◽  
Author(s):  
Hao Zhang ◽  
Changhua Jiang ◽  
Rui Wang ◽  
Long Zhang ◽  
Ruonan Gai ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Expression Profiles ◽  
Interaction Network ◽  
Differentially Expressed ◽  
Genes Encoding ◽  
Heat Response ◽  
Physiological Analysis ◽  
Japanese Gardens ◽  
Shock Proteins

Abstract Clematis species are commonly grown in western and Japanese gardens. Heat stress can inhibit many physiological processes mediating plant growth and development. The mechanism regulating responses to heat has been well characterized in Arabidopsis thaliana and some crops, but not in horticultural plants, including Clematis species. In this study, we found that Clematis alpina ‘Stolwijk Gold’ was heat-sensitive whereas Clematis vitalba and Clematis viticella ‘Polish Spirit’ were heat-tolerant based on the physiological analyses in heat stress. Transcriptomic profiling identified a set of heat tolerance-related genes (HTGs). Consistent with the observed phenotype in heat stress, 41.43% of the differentially expressed HTGs between heat treatment and control were down-regulated in heat-sensitive cultivar Stolwijk Gold, but only 9.80% and 20.79% of the differentially expressed HTGs in heat resistant C. vitalba and Polish Spirit, respectively. Co-expression network, protein–protein interaction network and phylogenetic analysis revealed that the genes encoding heat shock transcription factors (HSFs) and heat shock proteins (HSPs) played an essential role in Clematis resistance to heat stress. Ultimately, we proposed that two clades of HSFs may have diverse functions in regulating heat resistance from C. vitalba and CvHSFA2-2 could endow different host with high temperature resistance. This study provides first insights into the diversity of the heat response mechanisms among Clematis species.

scholarly journals Comparative Analysis of the Transcriptomes of Three Varieties Provides Insights Into the Diversity of the Heat Response Mechanisms in Clematis Species

2020 ◽  
Author(s):  
Hao Zhang ◽  
Changhua Jiang ◽  
Long Zhang ◽  
RuoNan Gai ◽  
Siyuan Peng ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Expression Levels ◽  
Protein Protein Interaction ◽  
Regulated Expression ◽  
Heat Response ◽  
Japanese Gardens ◽  
Shock Proteins

Abstract Background: Clematis species are commonly grown in western and Japanese gardens. Heat stress can inhibit many physiological processes mediating plant growth and development. The mechanism regulating responses to heat has been well characterized in Arabidopsis thaliana and some crops, but not in horticultural plants, including Clematis species. Results: In this study, we identified a heat-sensitive Clematis variety (Clematis alpina ‘Stolwijk Gold’) and two heat-tolerant Clematis varieties (Clematis vitalba and Clematis viticella ‘Polish Spirit’) based on heat-related physiological indices. The leaf transcriptomes under normal and heat stress conditions were analyzed by RNA sequencing. Additionally, heat tolerance-related genes (HTGs) were identified and their expression levels were analyzed. Following heat treatments, 41.67% of the differentially expressed HTGs in Stolwijk Gold had down-regulated expression levels, whereas only 9.80% and 21.36% of the differentially expressed HTGs in C. vitalba and Polish Spirit, respectively, had the same trend. The HTGs’ co-expression and protein–protein interaction networks revealed that the hub genes regulating Clematis resistance to heat stress encode heat shock transcription factors (HSFs) and heat shock proteins (HSPs). Moreover, the sensitivity of Stolwijk Gold to heat is mainly due to the heat-induced down-regulated expression of these genes. On the basis of phylogenetic and expression analyses, the differentially expressed HSF and HSP genes in the three examined varieties were divided into three and four clades, respectively, with similar expression profiles common among orthologous family members. Furthermore, we identified two HSF classes in C. vitalba that may have diverse functions influencing heat resistance. Conclusions: Our study provides insights into the diversity of the heat response mechanisms among Clematis species and may be useful for breeding new heat-resistant ornamental Clematis varieties.

scholarly journals Wheat (Triticum aestivum L.) TaHMW1D Transcript Variants Are Highly Expressed in Response to Heat Stress and in Grains Located in Distal Part of the Spike

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 687
Author(s):  
Chan Seop Ko ◽  
Jin-Baek Kim ◽  
Min Jeong Hong ◽  
Yong Weon Seo
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Grain Filling ◽  
High Temperature Stress ◽  
Two Dimensional Gel Electrophoresis ◽  
Transcript Variants

High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE) and peptide analyses were performed. Spots showing decreased contents in stressed plants were shown to have strong similarities with a high-molecular glutenin gene, TraesCS1D02G317301 (TaHMW1D). QRT-PCR results confirmed that TaHMW1D was expressed in its full form and in the form of four different transcript variants. These events always occurred between repetitive regions at specific deletion sites (5′-CAA (Glutamine) GG/TG (Glycine) or (Valine)-3′, 5′-GGG (Glycine) CAA (Glutamine) -3′) in an exonic region. Heat stress led to a significant increase in the expression of the transcript variants. This was most evident in the distal parts of the spike. Considering the importance of high-molecular weight glutenin subunits of seed storage proteins, stressed plants might choose shorter polypeptides while retaining glutenin function, thus maintaining the expression of glutenin motifs and conserved sites.

Localization of metallothionein, heat shock protein (Hsp70), and superoxide dismutase expression in Hemidiaptomus roubaui (Copepoda, Crustacea) exposed to cadmium and heat stress

2007 ◽  
Vol 85 (3) ◽  
pp. 362-371 ◽  
Author(s):  
Martine Liberge ◽  
Roxane-M. Barthélémy
Keyword(s):  
Superoxide Dismutase ◽  
Heat Stress ◽  
Heat Shock ◽  
Metal Binding ◽  
Stress Proteins ◽  
Reproductive Function ◽  
Cadmium Stress ◽  
Specific Distribution ◽  
Freshwater Crustacean ◽  
Shock Proteins

Immunohistochemical methods were applied in the present study to investigate the expression of stress proteins such as metallothioneins (MT), which are metal-binding proteins, and heat shock proteins (Hsp70), as well as an antioxidant enzyme (superoxide dismutase, SOD), in the freshwater crustacean copepod Hemidiaptomus roubaui (Richard, 1888) exposed to cadmium or heat stress. The results show a tissue-specific distribution of MT-like protein after cadmium exposure in the brain and in the nerve cord. Cadmium stress did not provoke inducible Hsp70 or SOD expression. Unlike cadmium, heat stress induced the expression of Hsp70 and SOD in the shell glands, a structure involved in the reproductive function, and more particularly in the formation of the diapause egg envelope. MT expression is not induced in animals exposed to heat stress.

scholarly journals Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in Lentinula Edodes Thermotolerance

2018 ◽  
Vol 50 (5) ◽  
pp. 1617-1637 ◽  
Author(s):  
Gang-Zheng Wang ◽  
Chao-Jun Ma ◽  
Yi Luo ◽  
Sha-Sha Zhou ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Indoleacetic Acid ◽  
Lentinula Edodes ◽  
Acid Biosynthesis ◽  
Over Expression ◽  
Indoleacetic Acid Biosynthesis ◽  
Shock Proteins ◽  
Exogenous Iaa

Background/Aims: Heat stress could cause huge losses for Lentinula edodes in China and other Asian cultivation areas. Yet our understanding of mechanism how to defend to heat stress is incomplete. Methods: Using heat-tolerant and heat-sensitive strains of L. edodes, we reported a combined proteome and transcriptome analysis of L. edodes response to 40 °C heat stress for 24 h. Meanwhile, the effect of LeDnaJ on the thermotolerance and IAA (indoleacetic acid) biosynthesis in L. edodes was analyzed via the over-expression method. Results: The proteome results revealed that HSPs (heat shock proteins) such as Hsp40 (DnaJ), Hsp70, Hsp90 and key enzymes involved in tryptophan and IAA metabolism process LeTrpE, LeTrpD, LeTam-1, LeYUCCA were more highly expressed in S606 than in YS3357, demonstrating that HSPs and tryptophan as well as IAA metabolism pathway should play an important role in thermotolerance. Over-expression of LeDnaJ gene in S606 strains showed better tolerance to heat stress. It was also documented that intracellular IAA accumulation of S606 (8-fold up) was more than YS3357 (2-fold up), and exogenous IAA enhanced L. edodes tolerance to heat stress. Conclusion: Our data support the interest of LeTrpE, LeDnaJ, tryptophan and IAA could play a pivotal role in enhancing organism thermotolerance.

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