Genetic variation in fatty acid composition of fenugreek(Trigonella foenum-graecum L.) seed oil

Author(s):  
S. S. Rathore ◽  
S. N. Saxena ◽  
R. K. Kakani ◽  
L. K. Sharma ◽  
D. Agrawal ◽  
...  

An analysis has been carried out to explore the genetic variation in seventeen selected varieties of fenugreek. Sixteen fatty acids were identified in seed oil. Linoleic acid (18:2; n-6) and a-Linolenic acid (18:3; n-3) were the major contributor found in the range of 26-43% in variety Lam Selection. The MUFA content varied from 3.50% in Azad Methi to 19.31 % in Hisar Madhvi. The ratio of n-6 to n-3 fatty acids indicated that fenugreek seed lipids are a good source of PUFA. PCA identified linolenic and oleic acid as the most important traits responsible for variation in presented material and improving quality through breeding. Estimates of genetic distance values showed wide range of variation among the fenugreek genotypes. The Euclidean based UPGMA clustering revealed three real and four singleton clusters. Genetic diversity showed no relationship to geographical origin. Dissimilarities obtained showed a wide variation in oil content and in composition.

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 202
Author(s):  
Saqib Gulzar ◽  
Krisana Nilsuwan ◽  
Navaneethan Raju ◽  
Soottawat Benjakul

Shrimp oil (SO) rich in n-3 fatty acids and astaxanthin, mixed with antioxidant-rich tea seed oil (TSO), was microencapsulated using mung bean protein isolate and sodium alginate and fortified into whole wheat crackers. SO and TSO mixed in equal proportions were emulsified in a solution containing mung bean protein isolate (MBPI) and sodium alginate (SA) at varied ratios. The emulsions were spray-dried to entrap SO-TSO in MBPI-SA microcapsules. MBPI-SA microcapsules loaded with SO-TSO showed low to moderately high encapsulation efficiencies (EE) of 32.26–72.09% and had a fair flowability index. Two selected microcapsules with high EE possessed the particle sizes of 1.592 and 1.796 µm with moderate PDI of 0.372 and 0.403, respectively. Zeta potential values were −54.81 mV and −53.41 mV. Scanning electron microscopic (SEM) images indicated that microcapsules were spherical in shape with some shrinkage on the surface and aggregation took place to some extent. Fourier transform infrared (FTIR) and differential scanning calorimetry (DSC) analyses of samples empirically validated the presence of SO-TSO in the microcapsules. Encapsulated SO-TSO showed superior oxidative stability and retention of polyunsaturated fatty acids (PUFAs) to unencapsulated counterparts during storage of 6 weeks. When SO-TSO microcapsules were fortified in whole wheat crackers at varying levels (0–10%), the crackers showed sensorial acceptability with no perceivable fishy odor. Thus, microencapsulation of SO-TSO using MBPI-SA as wall materials could be used as an alternative carrier system, in which microcapsules loaded with PUFAs could be fortified in a wide range of foods.


2015 ◽  
Vol 140 (4) ◽  
pp. 362-372 ◽  
Author(s):  
Joyce W. Ngure ◽  
Chunyan Cheng ◽  
Shuqiong Yang ◽  
Qunfeng Lou ◽  
Ji Li ◽  
...  

Cucumber (Cucumis sativus) seed oil has the potential for use as an edible oil and as a pharmaceutical, cosmetic, insecticidal, and industrial product. In this study, we investigated, for the first time, the effect of cultivar and season on seed number, oil content, and fatty acid profiles as well as their proportions in different cucumber cultivars. We examined the effects of spring and autumn seasons on seed oil content and fatty acid composition in 46 cucumber cultivars and one wild species of cucumber (C. anguria) grown in greenhouse experiments in 2013 and 2014. Seed oil was determined using the Soxhlet method and fatty acids using the gas chromatography-mass spectrometry method. Seed oil content in the cucumber seeds ranged from 41.07% in ‘Hazerd’ to 29.24% in ‘Lubao’ while C. anguria had 23.3%. Fatty acids detected were linoleic (C18:2), palmitic (C16:0), oleic (C18:1), stearic (C18:0), linolenic (C18:3), behenic (C22:0), arachidic C20:0), lignoceric (C24:0), eicosenoic (C20:1), palmitoleic (C16:1), and myristic (C14:0), among other unidentified fatty acids. The results showed significant effects of cultivar genotype, growing season, and interactions on the variables examined. The content of seed oil and fatty acids differed significantly among the cultivar genotypes. Spring-grown cucumbers had higher quantities of oil than the autumn-grown cucumbers. The content of fatty acids (mainly palmitic, palmitoleic, stearic, oleic, eicosenoic, and lignoceric) also was higher in spring. In autumn there were more seeds, and higher linoleic, linolenic, and other unspecified fatty acids. The higher the oleic acid content the lower was the linoleic acid indicating a strong negative relationship in these two fatty acids. The higher the seed oil content the higher was linoleic and oleic indicating a positive relationship between the seed oil and the two fatty acids. Results of this study provide important information applicable in improving management and production of cucumber seed oil especially considering its versatility in uses. Furthermore, the wide range of fatty acids found in the studied cucumber cultivars could be used in the production of novel industrial oils through genetic engineering.


2011 ◽  
Vol 60 (6) ◽  
pp. 301-311 ◽  
Author(s):  
I. Ovando-Medina ◽  
FJ. Espinosa-García ◽  
J. Núñez-Farfán ◽  
M. Salvador-Figueroa

1970 ◽  
Vol 29 ◽  
pp. 65-67
Author(s):  
Md Kamrul Ahsan ◽  
SM Rahman

An investigation was conducted with ten exotic varieties of silkworm, Bombyx mori L. to estimate variability, heritability and genetic advance for nine quantitative characters. A wide range of variation was observed for the majority of the traits and a major portion of the total phenotypic variation was of genetic in nature. The maximum phenotypic and genotypic coefficient of variation was found in filament length (21.45, 21.44) and the minimum in hatching percentage (1.52, 1.44), respectively. A moderate to high heritability was recorded for these varieties. Filament length showed the highest heritability (99.98). A comparatively high heritability accompanied by a high genetic advance was also noted for majority of the traits. Substantial variability, high heritability and high genetic advance for filament length, weight of mature larvae, total number of eggs laid per female and effective rate of rearing by weight recorded in this study would afford scope for effective selection.   Key words: Bombyx mori; genetic variation; exotic varieties. DOI: http://dx.doi.org/10.3329/ujzru.v29i1.9469 UJZRU 2010; 29(1): 65-67


1994 ◽  
Vol 29 (3) ◽  
pp. 207-209 ◽  
Author(s):  
H. Puzicha

Effluents from point sources (industries, communities) and diffuse inputs introduce pollutants into the water of the river Rhine and cause a basic contaminant load. The aim is to establish a biological warning system to detect increased toxicity in addition to the already existing chemical-physical monitoring system. To cover a wide range of biocides, continuous working biotests at different trophic levels (bacteria, algae, mussels, water fleas, fishes) have been developed and proved. These are checked out for sensitivity against toxicants, reaction time, validity of data and practical handling under field conditions at the river. Test-specific appropriate methods are found to differentiate between the normal range of variation and true alarm signals.


Author(s):  
Bruce Walsh ◽  
Michael Lynch

One of the major unresolved issues in quantitative genetics is what accounts for the amount of standing genetic variation in traits. A wide range of models, all reviewed in this chapter, have been proposed, but none fit the data, either giving too much variation or too little apparent stabilizing selection.


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