OXYGEN-DEPENDENT MECHANISMS AND OXIDATIVE STRESS IN CHILDREN FOR FEMORAL HEAD OSTEOCHONDROPATHY

2019 ◽  
Vol 64 (10) ◽  
pp. 594-598
Author(s):  
M. P. Teplen’kiy ◽  
E. I. Kuznetsova ◽  
M. V. Chepeleva ◽  
E. S. Spirkina ◽  
S. N. Luneva ◽  
...  
Keyword(s):  
Catalase Activity ◽  
Phagocytic Activity ◽  
Stimulation Index ◽  
Oxidative Modification ◽  
Myeloperoxidase Activity ◽  
Secondary Products ◽  
Sod Activity ◽  
Aseptic Inflammation ◽  
Oxidative Modification Of Proteins ◽  
Nbt Test

Degenerative-dystrophic processes in the articular tissues are accompanied by the changes in the values of congenital immunity, being a result of immune responses to aseptic inflammation. The purpose of this study - to investigate the oxygen-dependent mechanisms of neutrophil phagocytic activity (NBT-test, myeloperoxidase activity), the biochemical values of lipid peroxidation, the oxidative modification of proteins and the activity of enzyme antioxidant protection in children (at the age of 12-16 years) with osteochondropathy of the femoral head.The increase in spontaneous NBT-test and myeloperoxidase content in neutrophils, the decrease in NBT stimulation index, as well as the statistically significant increase in the activity of superoxide dismutase, the main producer of hydroperoxide, was revealed in the group examined. At the same time, no increase in catalase activity and in the content of lipoperoxidation products was observed, however, redistribution of the products of oxidative modification of proteins with ketone predominance was found. In case of osteochondropathy development in children the activation of the oxygen-dependent mechanisms of neutrophil phagocytic activity is observed, as well as the increase in the number of the secondary products of protein peroxidation - ketones. An increase in SOD activity, with a simultaneous and significant decrease in catalase activity evidence of accumulation of hydroperoxide causing neutrophil activation and chemotaxis. The study data can be used for predicting osteonecrosis development as additional criteria when decision-making of the advisability of performing reconstructive surgeries of the joint.

scholarly journals LIPID PEROXIDATION AND OXIDATIVE MODIFICATION OF PROTEINS IN THE PATHOGENESIS OF TICK-BORNE ENCEPHALITIS AND IXODIC TICK-BORNE BORRELIOSIS

2021 ◽  
Author(s):  
Darya Barsukova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Secondary Products ◽  
Endemic Region ◽  
Oxidative Modification Of Proteins ◽  
Tick Borne Encephalitis ◽  
Vector Borne ◽  
Chelyabinsk Region

Ixodic tick-borne borreliosis occupies a leading place among natural focal vector-borne infections in the Chelyabinsk region, and tick-borne encephalitis is less common. In the pathogenesis of the vast majority of diseases known to date, non-specific pathochemical processes are of the greatest importance. One of the most significant metabolic processes of this kind is free radical oxidation. Objective: to study the etiological structure and clinical features of tick-borne encephalitis and ixodic tick-borne borreliosis in comparison with the characteristics of the manifestations of oxidative stress in various clinical forms of tick-borne infections in the endemic region (for example, the Chelyabinsk region). Methods: Clinical studies were conducted on the basis of the Department of Infectious Diseases of the city Center of Neuroinfections, the 1st and 4th infectious departments of the MAUZ OZP GKB No. 8 in Chelyabinsk. The diagnosis of CE, ICB was established after a comprehensive clinical and laboratory study. The content of carbonyl products of oxidative modification of proteins was evaluated by their reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH). In determining the content of primary and secondary products of lipid peroxidation, a method based on the phenomenon of rearrangement of double bonds into diene conjugates was used. Conclusions: The leading place among natural-focal vector-borne infections in the Chelyabinsk region is occupied by ICB, and CE is less common. The revealed changes in the study of oxidative stress in patients with CE and ICB not only indicate the prospects for developing new approaches to the pathogenetic therapy of tick-borne infections, involving the use of antioxidants in complex therapy, but also suggest a greater effectiveness of direct-acting lipophilic antioxidants that limit diene conjugation, and thereby prevent the accumulation of secondary cytotoxic products of lipid peroxidation.

The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Modification ◽  
Resistance Development ◽  
Oxidative Modification Of Proteins ◽  
Kidney Beans ◽  
Calorie Diet ◽  
Diet Control ◽  
High Calorie Diet ◽  
High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

THE INTENSITY OF OXIDATIVE MODIFICATION OF PROTEINS AND PEROXIDATION OF LIPIDS IN THE BLOOD SERUM, BRAIN, LIVER AND PANCREAS TISSUES OF RATS WITH INSULIN RESISTANCE AND CONGENITAL IODINE DEFICIENCY

2019 ◽  
pp. 115-120
Author(s):  
V. B. Stetsevyat ◽  
N. M. Voronych-Semchenko
Keyword(s):  
Insulin Resistance ◽  
Iodine Deficiency ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
High Fructose ◽  
Liver And Pancreas ◽  
Dependent Processes

The results of the study about the nature of oxygen-dependent processes in rats that were on a high-fructose diet for 8 weeks under conditions of adequate iodine supply and congenital iodine deprivation is presented in the article. The significant activation of peroxide oxidation of proteins (by increasing the aldo- and keto-derivates of a neutral nature) and lipids (increased of diene conjugates content and products, that are responsible to thiobarbituric acid) of animals with insulin resistance was found. An aggravating factor in the course of these processes is a congenital iodine deficiency. The changes of oxidative modification of proteins in animals with congenital iodine deficiency had multidirectional character, and processes of lipid peroxidation mainly increased. The most pronounced changes of the studied processes were observed in the liver and pancreas of animals with insulin resistance against the background of congenital iodine deficiency.

Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain

2006 ◽  
Vol 27 (1) ◽  
pp. 42-53 ◽  
Author(s):  
Minna A. Korolainen ◽  
Gundars Goldsteins ◽  
Tuula A. Nyman ◽  
Irina Alafuzoff ◽  
Jari Koistinaho ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Frontal Cortex ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins

scholarly journals Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sahoko Ichihara ◽  
Yuka Suzuki ◽  
Jie Chang ◽  
Kentaro Kuzuya ◽  
Chisa Inoue ◽  
...  
Keyword(s):  
Atp Synthesis ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins

Indicator Characterizing Carbonyl-Dependent Modification of Erythrocytic Superoxydismutase as a Biochemical Marker of Oxidative Stress in Coronary Heart Disease

Kardiologiia ◽  
2020 ◽  
Vol 60 (5) ◽  
pp. 57-61
Author(s):  
A. K. Tikhaze ◽  
V. Ya. Kosach ◽  
V. Z. Lankin ◽  
A. A. Panferova ◽  
M. D. Smirnova
Keyword(s):  
Oxidative Stress ◽  
Heart Disease ◽  
Stress Intensity ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Oxidative Modification ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Sod Activity ◽  
Stress Marker

Aim To study the oxidative modification of red blood cell Cu,Zn superoxide dismutase (SOD) in patients with ischemic heart disease (IHD) in vivo and in vitro to substantiate the use of a new oxidative stress marker.Material and methods Red blood cell Cu,Zn SOD was measured by depression of nitrotetrazolium blue reduction by the superoxide anion generated in xanthine oxidase xanthine oxidation. Red blood cell Cu,Zn SOD was measured immunochemically. The biochemical study was performed in the control group (patients with low extremity fracture without known history of cardiovascular diseases and hyperlipidemia) and in groups of patients with acute myocardial infarction, stable angina, and decompensated heart failure. For evaluation of oxidative stress intensity in IHD patients, an empirical SOD oxidative modification coefficient (OMCSOD) was proposed, which is a Cu,Zn SOD activity / Cu,Zn SOD content ratio.Results The red blood cell Cu,Zn SOD activity was significantly decreased in all IHD groups compared to the control group. Furthermore, OMCSOD was also considerably decreased in IHD patients, which warrants the use of this biochemical index as an oxidative stress marker.Conclusion It was shown that the Cu,Zn SOD modification was induced by interaction of the enzyme molecules with a natural dicarbonyl, malonic dialdehyde, and OMCSOD can be used for evaluation of oxidative stress intensity in IHD patients.

Effect of Cu/Zn-Superoxide Dismutase from the Fungal Strain Humicola lutea 103 on Antioxidant Defense of Graffi Tumor-Bearing Hamsters

2002 ◽  
Vol 57 (1-2) ◽  
pp. 197-204 ◽  
Author(s):  
Emilia Ivanova ◽  
Maria Angelova ◽  
Ludmila Slokoska ◽  
Svetlana Pashova ◽  
Reneta Toshkova ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Tumor Growth ◽  
Tumor Progression ◽  
Catalase Activity ◽  
Early Stage ◽  
Fungal Strain ◽  
Sod Activity ◽  
Tumor Bearing ◽  
Low Levels ◽  
Humicola Lutea

A novel Cu/Zn-containing superoxide dismutase (SOD) was isolated from the fungal strain Humicola lutea 103. Previously, a protective effect of this enzyme (HLSOD) against tumor growth and also superoxide production in Graffi tumor-bearing hamsters (TBH) were established. The aim of the present study was to investigate the effect of HLSOD on the activity of endogenous SOD and catalase in the cells from TBH during tumor progression. Our results point out that transplantation of Graffi tumor causes a significant decrease in SOD activity in the cells from liver of the hosts (from 35 to 59% compared to the control). In the tumor cells relatively low levels of SOD (about 7 U mg protein-1) were found, and Cu/ZnSOD was the main isoenzyme in total SOD activity. Tumor growth resulted in a reduction of catalase activity, which correlated with the process of tumor progression. A single dose (65 U) treatment with HLSOD caused an increase in endogenous SOD and catalase activity in healthy animals and resulted in restoration of the antioxidant ability in liver cells of the hosts at the early stage of tumor progression. The results show the possible participation of HLSOD in the host oxidant-antioxidant balance, which is probably one of the factors of its immunoprotective action established earlier.

Oxidative modification of proteins and phospholipids in rotenone-induced neurotoxicity in PC12 cells

2016 ◽  
Vol 96 ◽  
pp. S68
Author(s):  
Elisabetta Chiaradia ◽  
Fiorella D'Onofrio ◽  
Angela Gambelunghe ◽  
Lorena Urbanelli ◽  
Carla Emiliani ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins

scholarly journals PROSPECTS FOR THE USE OF THE PARAMETERS OF OXIDATIVE MODIFICATION OF SERUM PROTEINS FOR ASSESSMENT OF THE AGONAL PERIOD DURATION

2019 ◽  
Vol 5 (3) ◽  
pp. 28-32
Author(s):  
I. S. Edelev ◽  
L. M. Obukhova ◽  
N. A. Andriyanova ◽  
N. S. Edelev
Keyword(s):  
Serum Proteins ◽  
Oxidative Modification ◽  
Evidence Based ◽  
Natural Death ◽  
Oxidative Modification Of Proteins ◽  
Carbonyl Derivatives ◽  
Forensic Examination ◽  
Autopsy Data ◽  
Period Duration ◽  
Relevant Factors

In the article we present results of the studies in cases of death with a long agonal period to determine the length of the agony.Objectives. The task was to examine the features of oxidative modification of serum proteins in cases of deaths with a long agonal period.Material and methods. In all experiments we analyzed the liquid blood sampled from 54 corpses of persons of both sexes who died from the violent and natural death in age of 13 to 89 years. The assessment of the oxidative modification of proteins (OMP) was performed by the level of carbonyl derivatives detected in the reaction with 2,4-dinitrophenylhydrazine. The levels of absolute and relative (in terms of protein) total OMP in cases of long agonal period were compared with the counterpart levels in cases of the short agony. The duration of the agonу was determined using information about the circumstances of the case and investigative data with the permission of the authority ordered the forensic examination. The autopsy data also were taken into account.Results. We revealed the dependence of the OMP level on the duration of the agonal period. The total OMP (in terms of protein) in cases of long agony have decreased by 45.46 %. The data obtained have showed that the parameters of the OMP, taking into account all the relevant factors, can be used to develop criteria for establishing the duration of the agonal period as well as to increase the evidence-based significance of medico-legal conclusions.

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