scholarly journals LIPID PEROXIDATION AND OXIDATIVE MODIFICATION OF PROTEINS IN THE PATHOGENESIS OF TICK-BORNE ENCEPHALITIS AND IXODIC TICK-BORNE BORRELIOSIS

2021 ◽  
Author(s):  
Darya Barsukova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Free Radical Oxidation ◽  
Oxidative Modification ◽  
Secondary Products ◽  
Endemic Region ◽  
Oxidative Modification Of Proteins ◽  
Tick Borne Encephalitis ◽  
Vector Borne ◽  
Chelyabinsk Region

Ixodic tick-borne borreliosis occupies a leading place among natural focal vector-borne infections in the Chelyabinsk region, and tick-borne encephalitis is less common. In the pathogenesis of the vast majority of diseases known to date, non-specific pathochemical processes are of the greatest importance. One of the most significant metabolic processes of this kind is free radical oxidation. Objective: to study the etiological structure and clinical features of tick-borne encephalitis and ixodic tick-borne borreliosis in comparison with the characteristics of the manifestations of oxidative stress in various clinical forms of tick-borne infections in the endemic region (for example, the Chelyabinsk region). Methods: Clinical studies were conducted on the basis of the Department of Infectious Diseases of the city Center of Neuroinfections, the 1st and 4th infectious departments of the MAUZ OZP GKB No. 8 in Chelyabinsk. The diagnosis of CE, ICB was established after a comprehensive clinical and laboratory study. The content of carbonyl products of oxidative modification of proteins was evaluated by their reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH). In determining the content of primary and secondary products of lipid peroxidation, a method based on the phenomenon of rearrangement of double bonds into diene conjugates was used. Conclusions: The leading place among natural-focal vector-borne infections in the Chelyabinsk region is occupied by ICB, and CE is less common. The revealed changes in the study of oxidative stress in patients with CE and ICB not only indicate the prospects for developing new approaches to the pathogenetic therapy of tick-borne infections, involving the use of antioxidants in complex therapy, but also suggest a greater effectiveness of direct-acting lipophilic antioxidants that limit diene conjugation, and thereby prevent the accumulation of secondary cytotoxic products of lipid peroxidation.

scholarly journals Study of prooxidant activity and some indicators of the pituitary-gonadal system in patients suffering from chronic endometritis

2020 ◽  
Vol 22 (1) ◽  
pp. 66-69
Author(s):  
N V Kirillova ◽  
O M Spasenkova ◽  
A G Platonova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Modification ◽  
Aliphatic Aldehyde ◽  
Oxidative Modification ◽  
Control Group ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
Chronic Endometritis ◽  
Oxidative Protein Modification

The state of lipid peroxidation and the oxidative modification of proteins in the blood of 29 women with chronic endometritis aged 27 to 40 years were studied. The control group consisted of 19 healthy women aged 27 to 44 years. The intensity of lipid peroxidation was estimated by the level of diene conjugates and malondialdehyde, the oxidative modification of proteins - by the level of carbonyl derivatives. It was shown that in the blood of women with chronic endometritis, the concentration of aliphatic aldehyde groups and 1,9 times the carbonyl groups in protein molecules increases by 3,3 times compared with the control group. A significant increase in diene conjugates by 34% and malondialdehyde in blood plasma and red blood cells by 47 and 43%, respectively, was established. The increase in lipid peroxidation products and oxidative protein modification in the blood indicates the development of oxidative stress in women suffering from chronic endometritis. Compared with healthy patients with chronic endometritis, a convincing tendency toward an increase in prolactin and anti- Muller hormone against a decrease in progesterone was revealed in the group compared to healthy ones. To predict possible complications in patients with chronic endometritis, it is advisable to study the indicators of protein and lipid peroxidation as markers of oxidative stress and the severity of the inflammatory process. In addition, such patients should be advised to take antioxidant drugs in combination with the basic therapy.

The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Modification ◽  
Resistance Development ◽  
Oxidative Modification Of Proteins ◽  
Kidney Beans ◽  
Calorie Diet ◽  
Diet Control ◽  
High Calorie Diet ◽  
High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

scholarly journals Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Muscle Tissue ◽  
Salmo Trutta ◽  
Protein Carbonyl ◽  
Oxidative Modification ◽  
Oxidative Stress Biomarkers ◽  
Development Stages ◽  
Stress Biomarkers ◽  
Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

Effect of sucrose and glucose on oxidative modification of proteins upon heat stress in Arabidopsis thaliana cat2cat3 knockout mutant

2020 ◽  
Vol 12 (2) ◽  
pp. 150-155
Author(s):  
Inna Buzduga ◽  
Tetiana Tkachuk ◽  
Irina Panchuk
Keyword(s):  
Oxidative Stress ◽  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
Potassium Phosphate ◽  
Oxidative Modification ◽  
Soluble Carbohydrates ◽  
Carbonyl Groups ◽  
Wild Type ◽  
Knockout Mutant ◽  
Oxidative Modification Of Proteins

High temperature negatively affects the plants. In particular, under the heat stress he production of reactive oxygen species increases in the plant cell. It leads to the development of oxidative stress. The formation of carbonyl groups in proteins is a marker of oxidative damage of plant cells. Plants have a defense system that consists of soluble carbohydrates such as sucrose and glucose and antioxidant enzymes, including catalase. Carbohydrates have protective mechanisms and can activate different signaling pathways with following changes in gene expression. Despite the data available, information on the effects of sucrose and glucose on the oxidative modification of proteins under heat stress is insufficient. The aim of our work was to study the role of sucrose and glucose for the carbonyl groups content in cat2cat3 knockout plants of A. thaliana under heat stress. We used 7-week-old Arabidopsis thaliana plants of wild-type and knockout cat2cat3 line, which lacks the expression of two catalase genes – cat2 and cat3. Plants were grown under 16-hour light day at a temperature of + 20°C and an illumination of 2.5 kL. Heat treatment was performed on a water bath in glass flasks with 15-20 leaves which were incubated in 1 mm potassium phosphate buffer without carbohydrates and with addition of sucrose or glucose (1% final concentration) during 2 and 4 hours at the +37°C and +44° C. The content of carbonyl groups and total protein was determined photometrically. It has been shown that intact knockout cat2cat3 plants have a higher content of carbonyl groups, which indicates chronic oxidative stress. Addition of exogenous sucrose or glucose to the incubation buffer had a protective effect during 4 hours of stress. Carbonyl groups formation in wild type decreased under the +37ºС and +44ºС, while in the cat2сat3 line only under the moderate (+37ºС) heat stress. In the knockout mutant alternative ways of defense are exhausted under +44ºC.

The level of carbonylation of plasma proteins and peripheral blood leukocytes in patients with different duration of Alzheimer’s disease

2021 ◽  
pp. 235-243
Author(s):  
Petrov Dmitriy Sergeevich Petrov Dmitriy Sergeevich ◽  
Mariya Germanovna Engalycheva ◽  
Natalya Vasilievna Korotkova ◽  
Ulugbek Usmanovich Ochilov
Keyword(s):  
Oxidative Stress ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Blood Plasma ◽  
Peripheral Blood ◽  
Plasma Proteins ◽  
Oxidative Modification ◽  
Mononuclear Leukocytes ◽  
Adaptive Potential ◽  
Oxidative Modification Of Proteins

The course of Alzheimer’s disease is associated with an increase in oxidative stress associated with an increase in the production of reactive oxygen species against the background of neurodegenerative inflammation, and a simultaneous depletion of the antioxidant defense capabilities of brain cells. The result is the oxidative modification of macromolecules: proteins, lipids, nucleic acids. Protein carbonylation products accumulate not only in neurons, and in direct correlation with the degree of increase in amyloidosis and neurodegeneration, but also in extra-neuronal tissues, including leukocytes. In the course of this study, the levels of spontaneous and induced oxidative modification of proteins were determined in the blood plasma and fractionated leukocytes of peripheral blood of patients with different durations of Alzheimer’s disease, and the value of the reserve-adaptive potential was assessed as markers of the severity of oxidative stress. It has been established that the course of Alzheimer’s disease has a greater effect on the accumulation of carbonyl derivatives in blood plasma. In patients with a disease duration of 5–10 years, the total level of aldehyde and ketone derivatives of modified plasma proteins exceeds the same indicator in subgroups with a shorter duration of the disease. This tendency is less typical for mononuclear leukocytes. The level of induced oxidative modification of proteins increases to a greater extent in blood plasma than in fractionated leukocytes. This indicates the depletion of the reserve-adaptive potential of plasma antioxidant capabilities, which is more pronounced in patients with a long course of Alzheimer’s disease. For polymorphonuclear leukocytes, such a pattern was not revealed, which is probably associated with a short cell life. In mononuclear leukocytes, as well as in plasma, there is a tendency to depletion of the reserve-adaptive potential, but to a lesser extent.

scholarly journals Correction of redox metabolism and the relationship of markers of systemic chronic inflammation with a measure of overall survival in cancer patients with multiorgan resections

2021 ◽  
Vol 17 (4) ◽  
pp. 74-79
Author(s):  
Ye.V. Shulga
Keyword(s):  
Lipid Peroxidation ◽  
Overall Survival ◽  
Intensive Care ◽  
Cancer Patients ◽  
Tumor Necrosis ◽  
Oxidative Modification ◽  
Necrosis Factor Alpha ◽  
Oxidative Modification Of Proteins ◽  
Factor Alpha ◽  
Necrosis Factor

Background. According to the literature, multiorgan resections are carried out with the most radical removal of healthy tissue. Therefore, there is a need for the most favorable preparation of the patient with perioperative therapy to reduce further complications with obtaining improved clinical and pathological prognostic indicators to increase the overall survival. Purpose: correction of redox metabolism based on the data of the oxidative modification of proteins, degree of their catabolic reaction, and data on the enzymatic state and lipid peroxidation in cancer patients with multiorgan resections on the gastrointestinal tract under conditions of perioperative intensive care. Materials and methods. The study involved 117 cancer patients aged 67.6 ± 3.7 years with locally advanced tumor processes of the gastrointestinal tract. Studies have been conducted on the enzymatic state, lipid peroxidation and oxidative modification of proteins, both in terms of the degree of fragmentation of their products in spontaneous and iron-induced reactions, and the level of medium-weight protein molecules. Serum levels of circulating tumor necrosis factor alpha, interleukin-6 and -8 were determined. Survival analysis was performed using the Kaplan-Meier method. Results. The study of patients has shown an improvement in the indicators of oxidative modification of proteins and lipid peroxidation. Survival in cancer patients with multiorgan resections demonstrated dependence on the levels of tumor necrosis factor alpha, interleukin-6 and -8 in the blood serum. Conclusions. Conducting perioperative intensive care makes it possible to reliably restore indicators of the enzymatic state, lipid peroxidation and oxidative protein modification products (on the fourth day and second week, p < 0.05). This gives us grounds for recommending the determination of these parameters in the group of cancer patients and the implementation of metabolic intensive care. In the group of L-ornithine L-aspartate and quercetin combination with povidone, the lowest indicators of systemic chronic inflammation and the best overall survival were found.

scholarly journals Oxidative metabolism of neutrophils in patients with community-acquired pneumonia

2016 ◽  
Vol 29 (1) ◽  
pp. 5-7
Author(s):  
Lyudmila Andreevna Demidchik ◽  
Valentina Vitalyevna Lee ◽  
Larissa Yevgenyevna Muravlyova ◽  
Vilen Borisovich Molotov-Luchanskiy ◽  
Ryszhan Yemelyevna Bakirova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Advanced Oxidation ◽  
Community Acquired Pneumonia ◽  
Oxidative Modification ◽  
Control Group ◽  
Advanced Oxidation Protein Products ◽  
Oxidative Modification Of Proteins ◽  
Moderate Severity ◽  
Carbonyl Derivatives ◽  
Derivatives Of

Abstract At the present time, available views show our limited knowledge of the peculiarities of the functional status of neutrophils and their metabolism in patients with community-acquired pneumonia (CAP). The studying of changes of metabolic status of neutrophils can broaden our views about pneumonia pathogenesis and define datum points of therapeutic effect. Purpose of our research: to define oxidative stress activity and the level of oxidative modification of proteins of neutrophils in CAP patients. Materials and methods: neutrophils obtained from 23 patients with community-acquired pneumonia. Control group consisting of 19 healthy volunteers. The reactive carbonyl derivatives of proteins and advanced oxidation protein products were defined so as to assess the oxidative damage of proteins. The malondialdehyde and nitrite ions were assessed as being indicators of the oxidative stress. The neutrophils of CAP patients with moderate severity were characterized by a tendency of evidencing decreasing content of advanced oxidation protein products, along with the statistically important enhanced levels of carbonyl derivatives and nitrite ions, while their malondialdehyde status practically leveled off with the control and had only an insignificant trend towards growth. We have demonstrated the accumulation of carbonyl derivatives and nitrite ions in the peripheral neutrophils of CAP patients. These results give evidence of an oxidative misbalance in the cells which contributes to the aggravation of the disease.

scholarly journals New Markers of Oxidative Damage to Macromolecules

2008 ◽  
Vol 27 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Emina Čolak
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Free Radicals ◽  
Oxidative Damage ◽  
Protein Modification ◽  
Lipid Hydroperoxide ◽  
The Body ◽  
Oxidative Modification ◽  
Toxic Effects ◽  
Specific Marker

New Markers of Oxidative Damage to Macromolecules The presence of free radicals in biological material has been discovered some 50 years ago. In physiological conditions, free radicals, in the first place the ones of oxygen and nitrogen, are continuously synthesized and involved in the regulation of a series of physiological processes. The excess of free radicals is efficiently eliminated from the body in order to prevent their toxic effects. Toxic effects of free radicals may be classified into three groups: a) change of intracellular redox potential, b) oxidative modification of lipids, proteins and DNA, and c) gene activation. Lipid peroxidation involving cell membranes, lipoproteins and other molecules leads to the production of primary high-reactive intermediaries (alkyl radicals, conjugated dienes, peroxy- and alkoxyl radicals and lipid hydroperoxide), whose further breakdown generates the secondary products of lipid peroxidation: short-chain evaporable hydrocarbons, aldehydes and final products of lipid peroxidation: isoprostanes, MDA, 4-hydroxy-2, 3-transnonenal and 4,5-dihydroxydecenal which are important mediators of atherosclerosis, coronary disease, acute myocardial infarction, rheumatoid arthritis, systemic sclerosis and lupus erythematodes. Oxidative modification of proteins is manifested by changes in their primary, secondary and tertiary structures. Proteins have a specific biological function, and therefore their modification results in unique functional consequences. The nature of protein modification may provide valid information on the type of oxidants causing the damage. Chlorotyrosyl is a specific marker of oxidative damage to tyrosine caused by HOCl action, which most commonly reflects the involvement of neutrophils and monocytes in oxidative stress, while nitrotyrosyl indicates the presence of higher peroxy-nitrite synthesis. Methyonin and cysteine are the amino acids most sensitive to oxidative stress, carbonyl groups are markers of severe damage caused by free radicals, and di-tyrosyl is the most significant and sensitive marker of oxidative modification made by γ rays. >Carbonyl stress< is an important form of the secondary oxidation of proteins, where reducing sugars non-enzymatically react with amino groups of proteins and lipids and give rise to the production of covalent compounds known as advanced glycosylated end products (AGE-products). A hydroxyl radical damages the DNA, leading to a loss of base and the formation of abasic sites (AP sites), break of DNA chain and sugar modification. Final lipid peroxidation products (MDA) may covalently bind to DNA, producing the >DNA radicals< which are responsible for mutations. Measurement of an adequate oxidative stress biomarker may not only point to an early onset of disease, its progression and assessment of therapy effectiveness, but can also help in the clarification of the pathophysiological mechanisms of tissue damage caused by oxidative stress, prediction of disease prognosis and choice of appropriate treatment in the early stages of disease.

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