ROLE OF ERYTHROCYTES IN MECHANISMS OF NONSPECIFIC PROTECTION OF BLOOD IN INFECTION CAUSED BY THE FUNGUS OF GENUS PAECILOMYCES

2019 ◽  
Vol 64 (11) ◽  
pp. 677-680
Author(s):  
V. M. Akhunov ◽  
Zh. M. Sizova ◽  
László Galgóczi ◽  
A. M. Akhunova ◽  
T. P. Lavrentyeva
Keyword(s):  
Infectious Agent ◽  
Enzymatic Oxidation ◽  
Paecilomyces Variotii ◽  
Old Patients ◽  
Closed Cavity ◽  
Microbiological Methods ◽  
New Type ◽  
Human Infections ◽  
Permanent Formation

Paecilomyces variotii is a commonly occurring species in air and food, and it is also associated with many types of human infections. Tissue forms of the fungus Paecilomyces variotii or their cytoskeletons were revealed in the cytoplasm of erythrocytes in patients with allergy and bronchial asthma in paecilomycosis. Our study was aimed at investigating the role of red blood cells in the mechanisms of the nonspecific protection of the host in conditions of chronic persistent infection of the blood with the fungus of the genus Paecilomyces. We examined a total of eighty-four 16-to-72-year-old patients (39 men and 45 women) presenting with activation of paecilomyces infection in blood. We used laboratory, biochemical, allergic-and-immunological and microbiological methods of study. Fungal cultures were identified phenotypically and by means of phylogenetic analysis.Our findings are suggestive of a new type of the oxygen-dependent mechanism of cytotoxicity of erythrocytes, which is caused by permanent formation of reactive oxygen species as a result of non-enzymatic oxidation of haemoglobin to methaemoglobin. The resulting superoxide anion radical (O2-), hydrogen peroxide (H2O2), and hydroxyl radical (OH-) exhibit a powerful bactericidal action which is, probably, activated when the fungal cells are captured and immersed in the erythrocyte cytoplasm or in a closed cavity formed by RBCs around large fungal cells. In conditions of chronic blood infection with tissue forms of fungi of the genus Paecilomyces oxygen-dependent cytotoxicity of erythrocytes is the main mechanism of readjustment of blood from the infectious agent of Paecilomycosis.

Questions on the role of biofilms for the adaptation of microorganisms to unfavorable environmental factors by the example of P. aeruginosa

2020 ◽  
Vol 99 (4) ◽  
pp. 379-383
Author(s):  
Vasily N. Afonyushkin ◽  
N. A. Donchenko ◽  
Ju. N. Kozlova ◽  
N. A. Davidova ◽  
V. Yu. Koptev ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Environmental Factors ◽  
Barrier Function ◽  
Infectious Agent ◽  
Antagonistic Activity ◽  
The Body ◽  
Main Function ◽  
Pathogenic Potential ◽  
The Face

Pseudomonas aeruginosa is a widely represented species of bacteria possessing of a pathogenic potential. This infectious agent is causing wound infections, fibrotic cystitis, fibrosing pneumonia, bacterial sepsis, etc. The microorganism is highly resistant to antiseptics, disinfectants, immune system responses of the body. The responses of a quorum sense of this kind of bacteria ensure the inclusion of many pathogenicity factors. The analysis of the scientific literature made it possible to formulate four questions concerning the role of biofilms for the adaptation of P. aeruginosa to adverse environmental factors: Is another person appears to be predominantly of a source an etiological agent or the source of P. aeruginosa infection in the environment? Does the formation of biofilms influence on the antibiotic resistance? How the antagonistic activity of microorganisms is realized in biofilm form? What is the main function of biofilms in the functioning of bacteria? A hypothesis has been put forward the effect of biofilms on the increase of antibiotic resistance of bacteria and, in particular, P. aeruginosa to be secondary in charcter. It is more likely a biofilmboth to fulfill the function of storing nutrients and provide topical competition in the face of food scarcity. In connection with the incompatibility of the molecular radii of most antibiotics and pores in biofilm, biofilm is doubtful to be capable of performing a barrier function for protecting against antibiotics. However, with respect to antibodies and immunocompetent cells, the barrier function is beyond doubt. The biofilm is more likely to fulfill the function of storing nutrients and providing topical competition in conditions of scarcity of food resources.

Toward a theory on workplaces for smart workers

Facilities ◽  
2019 ◽  
Vol 38 (3/4) ◽  
pp. 298-315
Author(s):  
Luisa Errichiello ◽  
Tommasina Pianese
Keyword(s):  
Web Sites ◽  
Participant Observation ◽  
Secondary Data ◽  
Primary Data ◽  
Innovative Design ◽  
Content Type ◽  
New Type ◽  
Smart Working ◽  
Research Propositions

Purpose The purpose of this paper is to identify the main features of smart work centers (SWCs) and show how these innovative offices would support the implementation of smart working and related changes in workspaces (“bricks”), technologies (“bytes”) and organizational practices (“behaviors”). Design/methodology/approach In this study, scientific literature is combined with white papers and business reports and visits to 14 workplaces, including offices designed as SWCs, co-working spaces, one telecenter, one accelerator and one fab lab. Primary data were collected through interviews with managers and users and non-participant observation, whereas secondary data included web-sites, brochures, presentations, press releases and official documents. Findings The authors developed research propositions about how the design of spaces and the availability of technology within SWCs would support the “bricks” and “bytes” levers of smart working. More importantly, the authors assumed that this new type of workplace would sustain changes in employees’ behaviors and managers’ practices, thus helping to overcome several challenges traditionally associated with remote working. Research limitations/implications The exploratory nature of the research only provides preliminary information about the role of SWCs within smart working programs. Additional qualitative and quantitative empirical investigation is required. Practical implications This study provides valuable knowledge about how the design of corporate offices can be leveraged to sustain the implementation of smart working. Originality/value This study advances knowledge on workplaces by focusing on an innovative design of traditional offices (SWC). It also lays the foundations for future investigation aimed at testing the developed propositions.

scholarly journals GWENA: gene co-expression networks analysis and extended modules characterization in a single Bioconductor package

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Gwenaëlle G. Lemoine ◽  
Marie-Pier Scott-Boyer ◽  
Bathilde Ambroise ◽  
Olivier Périn ◽  
Arnaud Droit
Keyword(s):  
Muscle Development ◽  
R Package ◽  
Topological Information ◽  
Old Patients ◽  
Network Modules ◽  
Extended Analysis ◽  
Networks Analysis ◽  
Underlying Processes

Abstract Background Network-based analysis of gene expression through co-expression networks can be used to investigate modular relationships occurring between genes performing different biological functions. An extended description of each of the network modules is therefore a critical step to understand the underlying processes contributing to a disease or a phenotype. Biological integration, topology study and conditions comparison (e.g. wild vs mutant) are the main methods to do so, but to date no tool combines them all into a single pipeline. Results Here we present GWENA, a new R package that integrates gene co-expression network construction and whole characterization of the detected modules through gene set enrichment, phenotypic association, hub genes detection, topological metric computation, and differential co-expression. To demonstrate its performance, we applied GWENA on two skeletal muscle datasets from young and old patients of GTEx study. Remarkably, we prioritized a gene whose involvement was unknown in the muscle development and growth. Moreover, new insights on the variations in patterns of co-expression were identified. The known phenomena of connectivity loss associated with aging was found coupled to a global reorganization of the relationships leading to expression of known aging related functions. Conclusion GWENA is an R package available through Bioconductor (https://bioconductor.org/packages/release/bioc/html/GWENA.html) that has been developed to perform extended analysis of gene co-expression networks. Thanks to biological and topological information as well as differential co-expression, the package helps to dissect the role of genes relationships in diseases conditions or targeted phenotypes. GWENA goes beyond existing packages that perform co-expression analysis by including new tools to fully characterize modules, such as differential co-expression, additional enrichment databases, and network visualization.

The Entrepreneurial University: Evidence of the Changing Role of Universities in Modern Russia

2014 ◽  
Vol 28 (4) ◽  
pp. 271-280 ◽  
Author(s):  
Dina Williams ◽  
Alexey Kluev
Keyword(s):  
Entrepreneurial University ◽  
Primary Data ◽  
Strategy Development ◽  
Infrastructure Development ◽  
Innovation Development ◽  
Policy Makers ◽  
Managerial Competencies ◽  
New Type ◽  
Changing Role

This paper examines the evolution of the entrepreneurial function of Russia's leading universities: the study is based on a conceptualization of a university's entrepreneurial development pathway developed by the OECD in 2009. The data on entrepreneurship development were collected in 2012 through a survey of technology transfer and innovation development managers of 18 National Research Universities. The primary data were complemented by desktop analysis of the strategy development documents of the universities surveyed. The results suggest that in the period studied, 2008–2012, a new type of university, the entrepreneurial university, has emerged in Russia. However, these entrepreneurial universities are facing serious challenges in the implementation of an entrepreneurial, innovation-oriented mission due to the lack of managerial competencies and inadequate infrastructure development. The paper has significant theoretical and practical implications in shedding light on the development of the entrepreneurial university in Russia, where the process is still in its infancy. The authors depict a trajectory of entrepreneurial transformation in and barriers to this process that, it is argued, university executives and policy makers should take into consideration.

Some Origins of Nationalism in East Africa

1968 ◽  
Vol 9 (1) ◽  
pp. 119-146 ◽  
Author(s):  
J. M. Lonsdale
Keyword(s):  
Central Government ◽  
Colonial Period ◽  
Political Activity ◽  
Colonial Rule ◽  
Face To Face ◽  
National Movements ◽  
New Type ◽  
First Time ◽  
Official Recognition

This paper attempts to provide a frame of reference for evaluating the role of ordinary rural Africans in national movements, in the belief that scholarly preoccupation with élites will only partially illumine the mainsprings of nationalism. Kenya has been taken as the main field of enquiry, with contrasts and comparisons drawn from Uganda and Tanganyika. The processes of social change are discussed with a view to establishing that by the end of the colonial period one can talk of peasants rather than tribesmen in some of the more progressive areas. This change entailed a decline in the leadership functions of tribal chiefs who were also the official agents of colonial rule, but did not necessarily mean the firm establishment of a new type of rural leadership. The central part of the paper is taken up with an account of the competition between these older and newer leaderships, for official recognition rather than a mass following. A popular following was one of the conditions for such recognition, but neither really achieved this prior to 1945 except in Kikuyuland, and there the newer leaders did not want official recognition. After 1945 the newer leadership, comprising especially traders and officials of marketing co-operatives, seems everywhere to have won a properly representative position, due mainly to the enforced agrarian changes which brought the peasant face to face with the central government, perhaps for the first time. This confrontation, together with the experience of failure in earlier and more local political activity, resulted in a national revolution coalescing from below, co-ordinated rather than instigated by the educated élite.

scholarly journals EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS

2017 ◽  
pp. 28-33 ◽  
Author(s):  
G. V. Demidova ◽  
E. P. Sokolova ◽  
V. P. Zyuzina ◽  
V. A. Rykova ◽  
I. V. Morozova ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Active Form ◽  
Infectious Agent ◽  
Toxic Shock ◽  
Toxic Activity ◽  
Virulent Strains ◽  
Extrachromosomal Elements ◽  
Live Bacteria ◽  
First Time

Aim. Elucidation of the role of extrachromosomal elements of heredity in manifestations of toxic properties of Yersinia pestis. Materials and methods. The study was carried out in vaccine strain Y. pestis EV76 (pMTl, pCDl, pPCPl) and non-plasmid variants of vaccine EV76 (pMTl\ pCDl', pPCPl') and virulent 231 (рМТГ, pCDl’, pPCPl') strains of Y. pestis. Presence of functionally active form of lipopolysaccharide (LPS) in the incubation medium of the bacteria was evaluated via toxicity of supernatant of Y. pestis for intact animals (infection-toxic shock) and mice sensitized by D-GalN. Results. 37°C cultures of Y. pestis EV76 containing a full amount of plasmids were established to release LPS into the environment. Non-plasmid variants of both vaccine and virulent strains of Y. pestis pMTl', pCD Г, рРСР 1 do not have this ability. Separation of LPS from cell wall was detected in live bacteria of plague infectious agent. This process is assumed to be coupled with translocation of proteins coded by pMTl, pCDl, pPCPl plasmids from the cell into the environment. Conclusion. Functional interconnection between extrachromosomal elements of heredity and toxic activity of Y. pestis LPS is established for the first time.

scholarly journals Biological role of zonulin: a biomarker of increased intestinal permeability syndrome

2021 ◽  
Vol 66 (1) ◽  
pp. 31-38
Author(s):  
A. I. Khavkin ◽  
N. M. Bogdanova ◽  
V. P. Novikova
Keyword(s):  
Digestive Tract ◽  
Intestinal Microbiota ◽  
Intestinal Mucosa ◽  
Inflammatory Process ◽  
Biological Rhythms ◽  
Infectious Agent ◽  
Biological Role ◽  
Pathological Conditions ◽  
Intercellular Connections

.Both changes in diet and pathological conditions caused by an infectious agent, allergic or autoimmune inflammatory process, affect the biological rhythms of the digestive tract, which negatively affects the intestinal microbiota and increases the permeability of the intestinal mucosa. The altered microbiota potentiates inflammation and causes a “vicious circle”. The zonulin protein is the agent that modulates the density of intercellular connections. The review presents data on the biological role of zonulin, correction of its synthesis violation with the help of functional products for baby food.

scholarly journals THE ROLE OF MALAY TRADITIONAL HOUSE AND RUMAH GADANG AS HOMESTAYS

2021 ◽  
Vol 8 (1) ◽  
pp. 125
Author(s):  
Rohaslinda Ramele ◽  
Jonny Wongso
Keyword(s):  
Rural Tourism ◽  
West Sumatra ◽  
New Type

In both Malaysia and Indonesia, traditional houses have been used as homestays, as a new type of rural tourism accommodation. This study aims to compare the implementation of rural tourism in Malaysia and Indonesia, to reveal the usage of traditional Malay house and Rumah Gadang as homestays in both countries, and to promote homestay as an alternative for rural tourism accommodation. Experiences on participating in both homestays in Kampung Lonek Homestay, Negeri Sembilan, Malaysia and Nagari Sumpur, West Sumatra, both majorly populated by the Minang community, are shared and analyzed to achieve these aims.   

scholarly journals Authorial Self and Modernity as Reflected in Diaries and Memoirs. Three 19th-Century Hungarian Case Studies

2019 ◽  
Vol 9 (1) ◽  
pp. 85-97
Author(s):  
Béla Mester
Keyword(s):  
Intellectual History ◽  
Social Role ◽  
Public Intellectual ◽  
Self Reflection ◽  
Individual Character ◽  
History Of ◽  
New Type ◽  
The Moment ◽  
The One

Abstract The role of the diaries and memoirs in the process of the conscious self-reflection and their contribution to the emergence of modern individual personalities are well-known facts of the intellectual history. The present paper intends to analyze a special form of the creation of modern individual character; it is the self-creation of the writer as a conscious personality, often with a clearly formulated opinion about her/his own social role. There will be offered several examples from the 19th-century history of the Hungarian intelligentsia. This period is more or less identical with the modernization of the “cultural industry” in Hungary, dominated by the periodicals with their deadlines, fixed lengths of the articles, and professional editing houses on the one hand and the cultural nation building on the other. Concerning the possible social and cultural role of the intelligentsia, it is the moment of the birth of a new type, so-called public intellectual. I will focus on three written sources, a diary of a Calvinist student of theology, Péter (Litkei) Tóth, the memoirs of an influential public intellectual, Gusztáv Szontagh, and a belletristic printed diary of a young intellectual, János Asbóth.

Sign in / Sign up

Export Citation Format

Share Document