Role of apoptosis in the course of traumatic disease in old patients with multiple trauma

Abstract Background Network-based analysis of gene expression through co-expression networks can be used to investigate modular relationships occurring between genes performing different biological functions. An extended description of each of the network modules is therefore a critical step to understand the underlying processes contributing to a disease or a phenotype. Biological integration, topology study and conditions comparison (e.g. wild vs mutant) are the main methods to do so, but to date no tool combines them all into a single pipeline. Results Here we present GWENA, a new R package that integrates gene co-expression network construction and whole characterization of the detected modules through gene set enrichment, phenotypic association, hub genes detection, topological metric computation, and differential co-expression. To demonstrate its performance, we applied GWENA on two skeletal muscle datasets from young and old patients of GTEx study. Remarkably, we prioritized a gene whose involvement was unknown in the muscle development and growth. Moreover, new insights on the variations in patterns of co-expression were identified. The known phenomena of connectivity loss associated with aging was found coupled to a global reorganization of the relationships leading to expression of known aging related functions. Conclusion GWENA is an R package available through Bioconductor (https://bioconductor.org/packages/release/bioc/html/GWENA.html) that has been developed to perform extended analysis of gene co-expression networks. Thanks to biological and topological information as well as differential co-expression, the package helps to dissect the role of genes relationships in diseases conditions or targeted phenotypes. GWENA goes beyond existing packages that perform co-expression analysis by including new tools to fully characterize modules, such as differential co-expression, additional enrichment databases, and network visualization.

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EARLY PROBIOTICS IN PREVENTING VENTILATOR-ASSOCIATED PNEUMONIA AFTER MULTIPLE TRAUMA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i10.38114 ◽

2020 ◽

pp. 83-85

Author(s):

TAMER HABIB ◽

AMIRA B KASSEM ◽

ISLAM AHMED

Keyword(s):

Multiple Trauma ◽

University Hospital ◽

Control Group ◽

Trauma Patients ◽

Ventilator Associated Pneumonia ◽

Mechanically Ventilated ◽

Significant Difference ◽

Multiple Trauma Patients ◽

And Control

Objective: Using probiotics in preventing ventilator-associated pneumonia (VAP) remain controversial due to different intensive care unit (ICU) populations included in such studies. The aim of this study is to evaluate the role of probiotics in prophylaxis of VAP after multiple trauma. Methods: Sixty-five adult multiple trauma patients on mechanical ventilator (expected ≥48 h) after admission to the Critical Care Medicine Department, Alexandria Main University Hospital from June to November 2018. Patients were randomly assigned using computer sheet into two groups; probiotics group (32 patients received one Lacteol Forte® sachet through orogastric/nasogastric tube 3 times daily during their ICU stay) and control group (33 patients received similar regimen of placebo sachets). All patients were followed up and subjected to all possible strategies of the diagnosis of microbiologically confirmed VAP. Results: Sixty-five patients were enrolled with a mean of age (39.48±7.692) years, 80% of them were male. Regarding the incidence of VAP, it was 18.46% of all patients without statistically significant difference between probiotics group (15.63%) and control group (21.21%) (p=0.751). Conclusion: Routine use of early probiotics in mechanically ventilated multiple trauma patients was not associated with lower incidence of VAP, duration of MV, or ICU mortality.

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Serum Derived from Multiple Trauma Patients Promotes the Differentiation of Endothelial Progenitor Cells In Vitro: Possible Role of Transforming Growth Factor-??1 and Vascular Endothelial Growth Factor165

Shock ◽

10.1097/01.shk.0000101669.49265.50 ◽

2004 ◽

Vol 21 (1) ◽

pp. 13-16 ◽

Cited By ~ 36

Author(s):

Dirk Henrich ◽

Patrick Hahn ◽

Matthias Wahl ◽

Kerstin Wilhelm ◽

Elisabeth Dernbach ◽

...

Keyword(s):

Growth Factor ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Multiple Trauma ◽

Transforming Growth Factor ◽

Trauma Patients ◽

Vascular Endothelial ◽

Multiple Trauma Patients

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Ultrasound assessment of the meniscus complex formation in health children and with the 1st stage of longitudinal platypodia ones

HIGHER SCHOOL’S PULSE ◽

10.5604/01.3001.0014.6947 ◽

2013 ◽

Vol 14 (SUPPLEMENT 1) ◽

pp. 1-5

Author(s):

Alexei Ivantsov ◽

Daria Vorobyeva

Keyword(s):

Joint Space ◽

Healthy Children ◽

Old Patients ◽

Shock Absorbers ◽

Keywords Meniscus ◽

Foot Arch ◽

Ultrasound Assessment ◽

Transformation Processes ◽

Ultrasound Investigation

Objectives: Foot arch reduces the exposure of weight and buf load due to its bufer function. in longitudinal platypodia foot looses this function and articular menisci have to take the role of the bufers (shock absorbers). Materials and Methods: Ultrasound assessment of meniscus thickness was performed on the joint space level. Results: varus type (43.4%) of the meniscus complex adaptation was dominant in 5-7 years old patients with the 1st stage longitudinal platypodia; symmetric type (51.9%) – in 8-12 years old patients; valgus type (56%) – in 13-17 years old ones. Healthy children showed the valgus type in all age periods. Conclusions: in our opinion the dominance of the varus type of the meniscus complex in 5-7 aged children and its further conversion into the valgus type in case of the 1st stage longitudinal platypodia refects the reduction of the lower extremities transformation processes from the varus into the most functional convenient valgus position at the age of 13-17. Keywords: meniscus, children, ultrasound investigation.

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The Impact of General Self-efficacy and the Severity of Malocclusion on Acceptance of Removable Orthodontic Appliances in 10- to 12-year-old Patients

10.21203/rs.3.rs-19887/v2 ◽

2020 ◽

Author(s):

Navid Naseri ◽

Tahereh Baherimoghadam ◽

Niloofar Bassagh ◽

Shahram Hamedani ◽

Elmira Bassagh ◽

...

Keyword(s):

Self Efficacy ◽

Orthodontic Treatment ◽

Self Report ◽

Orthodontic Appliances ◽

Old Patients ◽

Removable Orthodontic Appliances ◽

The Impact ◽

General Self Efficacy ◽

Patients Acceptance

Abstract Background: The patients’ acceptance of a treatment plan and their subsequent cooperation play a crucial role in achieving the best results in orthodontic treatments. Evidences show some personality traits such as general self-efficacy (GSE) and some dental traits such as severity of malocclusion are correlated with motivation of orthodontic treatment. These factors may predict the patients’ compliance and acceptance in using removable orthodontic appliances. This cross sectional study was conducted to assess the correlation of GSE and the severity of malocclusion with patients’ acceptance in using removable orthodontic appliances. Methods: This study recruited 50 patients aged 10-12 years who required removable orthodontic appliances. The severity of malocclusion was determined using the index of orthodontic treatment need (IOTN) before the onset of treatment and GSE of participants were assessed using GSE scale self-report. The acceptance questionnaire was proposed to the patients on first (T1), third (T2), and sixth (T3) month after the delivery of the appliance. Results: The GSE score had a statistically significant correlation with the total score of the acceptance questionnaire, subscale score of satisfaction with the appliance during eating and oral hygiene practice, duration of usage of the appliance, and interest in using it (P<0.05). The IOTN had no significant correlation with the acceptance questionnaire. Conclusions: Our findings substantiate the role of the GSES, concurrently declining the role of the IOTN in prediction of 10-12-year-old children's acceptance and cooperation in treatment of malocclusion with removable appliances.

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ROLE OF ERYTHROCYTES IN MECHANISMS OF NONSPECIFIC PROTECTION OF BLOOD IN INFECTION CAUSED BY THE FUNGUS OF GENUS PAECILOMYCES

Russian Clinical Laboratory Diagnostics ◽

10.18821/0869-2084-2019-64-11-677-680 ◽

2019 ◽

Vol 64 (11) ◽

pp. 677-680

Author(s):

V. M. Akhunov ◽

Zh. M. Sizova ◽

László Galgóczi ◽

A. M. Akhunova ◽

T. P. Lavrentyeva

Keyword(s):

Infectious Agent ◽

Enzymatic Oxidation ◽

Paecilomyces Variotii ◽

Old Patients ◽

Closed Cavity ◽

Microbiological Methods ◽

New Type ◽

Human Infections ◽

Permanent Formation

Paecilomyces variotii is a commonly occurring species in air and food, and it is also associated with many types of human infections. Tissue forms of the fungus Paecilomyces variotii or their cytoskeletons were revealed in the cytoplasm of erythrocytes in patients with allergy and bronchial asthma in paecilomycosis. Our study was aimed at investigating the role of red blood cells in the mechanisms of the nonspecific protection of the host in conditions of chronic persistent infection of the blood with the fungus of the genus Paecilomyces. We examined a total of eighty-four 16-to-72-year-old patients (39 men and 45 women) presenting with activation of paecilomyces infection in blood. We used laboratory, biochemical, allergic-and-immunological and microbiological methods of study. Fungal cultures were identified phenotypically and by means of phylogenetic analysis.Our findings are suggestive of a new type of the oxygen-dependent mechanism of cytotoxicity of erythrocytes, which is caused by permanent formation of reactive oxygen species as a result of non-enzymatic oxidation of haemoglobin to methaemoglobin. The resulting superoxide anion radical (O2-), hydrogen peroxide (H2O2), and hydroxyl radical (OH-) exhibit a powerful bactericidal action which is, probably, activated when the fungal cells are captured and immersed in the erythrocyte cytoplasm or in a closed cavity formed by RBCs around large fungal cells. In conditions of chronic blood infection with tissue forms of fungi of the genus Paecilomyces oxygen-dependent cytotoxicity of erythrocytes is the main mechanism of readjustment of blood from the infectious agent of Paecilomycosis.

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