Oral application of probiotic, prebiotic, and synbiotic in Pacific white shrimp(Litopenaeus vannamei)challenged with Vibrio harveyi

The use of antibiotics for controlling of luminous vibriosis caused by Vibrio harveyi is restricted now, because it induces antibiotic-resistant bacteria and leave residue in shrimp’s body. An alternative solution that can be done to treat the disease is by using applications of probiotic, prebiotic, and synbiotic. The aim of this research was to examine the effect of probiotic, prebiotic, and synbiotic on the survival rate and growth of Pacific white shrimp against V. harveyi infection. Feed as a treatment was supplemented with probiotic 1%, prebiotic 2%, and probiotic 1%+prebiotic 2% (synbiotic). Shrimps feed without supplementation of probiotic and prebiotic was used as a control treatment. The shrimps were maintainedin the aquarium (60×30×35 cm3) with a density of 40 shrimps/40 L and an average weight of 0.4±0.1 g. After 30 days of feeding treatment, the shrimp was challenged by immersion method with V. harveyi solution containing 106 CFU/mL. The results showed that before challenge, synbiotic feed treated shrimp has a growth rate (5.89%), feed conversionrate (1.21), and a high survival rate (80%). After challenge, survival rate (83.33%) of shrimp fed diet supplemented with synbiotic was higher than prebiotic (51.67%) and positive control (31.67%). Keywords: probiotic, prebiotic, synbiotic, Vibrio harveyi,Pacific white shrimp

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Microencapsulation of probiotics and its applications with prebiotic in Pacific white shrimp larvae through Artemia sp.

Jurnal Akuakultur Indonesia ◽

10.19027/jai.18.2.130-140 ◽

2019 ◽

Vol 18 (2) ◽

pp. 130-140

Author(s):

Dian Eka Ramadhani ◽

Widanarni Widanarni ◽

Sukenda Sukenda

Keyword(s):

Disease Resistance ◽

Immune Responses ◽

Population Growth ◽

Freeze Drying ◽

Vibrio Harveyi ◽

Pacific White Shrimp ◽

White Shrimp ◽

Immersion Method ◽

Mannan Oligosaccharide ◽

Growth Performances

ABSTRACTThis study aimed to produce microencapsulated probiotic Pseudoalteromonas piscicida (1Ub) and evaluate it with prebiotic mannan-oligosaccharide (MOS) through the enrichment of Artemia sp., on bacterial population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Microencapsulation of probiotic was done by the freeze-drying method. The shrimp larvae were reared for 13 days and fed by the Artemia sp. enriched with microcapsule of probiotic 1Ub (10 g/L), prebiotic MOS (12 mg/L), synbiotic, and control without administration of microencapsulated probiotic and prebiotic, including negative (C-) and positive (C+) control. On the day 14, all of the experimental shrimp larvae except C- were challenged through immersion method with Vibrio harveyi MR5339 (107 CFU/mL). This study showed that the administration of microcapsule of probiotic 1Ub, prebiotic MOS, and synbiotic through the enrichment of Artemia sp. could increase the bacteria population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Moreover, synbiotic treatment demonstrated the best result compared to other treatments.Keywords: probiotic, prebiotic, synbiotic, Pacific white shrimp, microencapsulation ABSTRAKPenelitian ini bertujuan untuk membuat mikrokapsul probiotik Pseudoalteromonas piscicida (1Ub) dan mengevaluasinya dengan prebiotik mannan-oligosaccharides (MOS) melalui pengayaan Artemia sp. terhadap populasi bakteri, performa pertumbuhan, respons imun dan resistensi penyakit pada larva udang vaname. Mikroenkapsulasi probiotik dilakukan dengan metode freeze-drying. Larva udang dipelihara selama 13 hari dan diberi pakan Artemia sp. yang telah diperkaya dengan mikrokapsul probiotik 1Ub (10 g/L), prebiotik MOS (12 mg/L), sinbiotik, dan kontrol tanpa penambahan mikrokapsul probiotik dan prebiotik, termasuk kontrol negatif (C-) dan positif (C+). Pada hari ke-14, seluruh larva udang percobaan kecuali C- diuji tantang melalui metode perendaman dengan Vibrio harveyi MR5339 (107 CFU/mL). Hasil penelitian menunjukkan bahwa pemberian mikrokapsul probiotik 1Ub, prebiotik MOS, dan sinbiotik melalui pengayaan Artemia sp. dapat meningkatkan populasi bakteri, performa pertumbuhan, respons imun, dan resistensi penyakit pada larva udang vaname. Selain itu, perlakuan sinbiotik menunjukkan hasil terbaik dibandingkan perlakukan lainnya.Kata kunci : probiotik, prebiotik, sinbiotik, udang vaname, mikroenkapsulasi

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Draft Genome Sequence of a Vibrio harveyi Strain Associated with Vibriosis in Pacific White Shrimp (Litopenaeus vannamei)

Genome Announcements ◽

10.1128/genomea.01662-16 ◽

2017 ◽

Vol 5 (7) ◽

Cited By ~ 5

Author(s):

Emille Moreno ◽

Marci Parks ◽

Lee J. Pinnell ◽

James J. Tallman ◽

Jeffrey W. Turner

Keyword(s):

Genome Sequence ◽

Litopenaeus Vannamei ◽

Vibrio Harveyi ◽

Draft Genome ◽

Penaeid Shrimp ◽

Pacific White Shrimp ◽

Draft Genome Sequence ◽

White Shrimp ◽

Content Type ◽

Future Studies

ABSTRACT Vibrio harveyi is a Gram-negative bacterium associated with vibriosis in penaeid shrimp. Here, we report the draft genome sequence of a V. harveyi strain isolated from Pacific white shrimp (Litopenaeus vannamei) during a vibriosis outbreak. The availability of this genome will aid future studies of vibriosis in shrimp aquaculture.

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Virulence properties and pathogenicity of multidrug-resistant Vibrio harveyi associated with luminescent vibriosis in pacific white shrimp, Penaeus vannamei

Journal of Invertebrate Pathology ◽

10.1016/j.jip.2021.107594 ◽

2021 ◽

pp. 107594

Author(s):

Wan Nurhafizah Wan Ibrahim ◽

Lee Kok Leong ◽

Laith Abdul Razzak ◽

Nadirah Musa ◽

Muhd Danish-Daniel ◽

...

Keyword(s):

Vibrio Harveyi ◽

Multidrug Resistant ◽

Pacific White Shrimp ◽

White Shrimp ◽

Penaeus Vannamei ◽

Virulence Properties

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Effect of Galangal (Alpinia galanga Linn.) Extract on the Growth Rate and Resistance to Vibrio harveyi and White Spot Diseases in Pacific White Shrimp (Litopenaeus vannamei)

Journal of Agricultural Science ◽

10.5539/jas.v7n9p117 ◽

2015 ◽

Vol 7 (9) ◽

Author(s):

Tidaporn Chaweepack ◽

Chutima Khomvilai ◽

Surachart Chaweepack ◽

Kaeko Kamei

Keyword(s):

Growth Rate ◽

Litopenaeus Vannamei ◽

Vibrio Harveyi ◽

Pacific White Shrimp ◽

White Spot ◽

White Shrimp ◽

Alpinia Galanga

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Effect of Red tilapia (Oreochromis mossambicus × Oreochromis niloticus) on growth and survival of the pacific shrimp (Litopenaeus vannamei) in simultaneous polyculture under marine conditions//Efecto de la tilapia roja (Oreochromis mossambicus × Oreochromis niloticus) en el crecimiento y supervivencia del camarón (Litopenaeus vannamei) en policultivo simultáneo bajo condiciones marinas

Biotecnia ◽

10.18633/biotecnia.v22i2.1237 ◽

2020 ◽

Vol 22 (2) ◽

pp. 5-12

Author(s):

Alejandro Mcdonal-Vera ◽

Wilfrido M. Contreras-Sánchez ◽

Neil J. Duncan ◽

Kevin Fitzsimmons ◽

Maria J. Contreras- García ◽

...

Keyword(s):

Litopenaeus Vannamei ◽

Oreochromis Niloticus ◽

Oreochromis Mossambicus ◽

Pacific White Shrimp ◽

White Shrimp ◽

Average Weight ◽

Growth And Survival ◽

Red Tilapia ◽

The Pacific ◽

The Mean

In the present investigation, we evaluated if the presence of tilapia affects growth and survival of shrimp during pre-growth and grow-out phases. In both experiments, we used a random-blocks design in a single earth pond (0.75 ha) that was prepared and divided into nine (7 x 30 m) enclosures that were considered to be three blocks of three enclosures each. Each randomly stocked block consisted on Pacific white shrimp Litopenaeus vannamei (30 shrimp/m2) and three different densities (0.0, 0.25 and 0.50 fish/m2) of red tilapia hybrids (Oreochromis mossambicus x Oreochromis niloticus) to give three different treatments with three replicates. The results from the pre-growth and grow-out experiments followed the same trend. In both cases, we found no significant statistical differences (P>0.05) in the final average weight of the shrimp. However, the experimental blocks had a significant (P<0.05) effect on the mean sample weight of shrimp. Shrimp in blocks B and C were significantly bigger than shrimp from block A (P<0.05). For both experiments, we found no effect of the presence of tilapias on survival or growth of shrimp.RESUMENEvaluamos si la presencia de tilapias libres afecta el crecimiento y la supervivencia de camarones en fase de preengorda y engorda. Para ambos experimentos, utilizamos un diseño de bloques al azar en un estanque rústico (0.75 ha) que fue preparado y dividido en nueve encierros (7 x 30 m), se formaron tres bloques con tres encierros cada uno. Cada encierro dentro de cada bloque fue aleatoriamente seleccionado para sembrar camarón blanco del Pacífico, Litopenaeus vannamei (30 camarones/m2) y tres diferentes densidades (0.0, 0.25 y 0.50 peces/m2) de híbrido de tilapia roja (Oreochromis mossambicus x Oreochromis niloticus) para formar tres tratamientos con tres réplicas. Los resultados del experimento de pre-engorda y engorda siguieron la misma tendencia. En ambos experimentos no encontramos diferencias estadísticamente en el crecimiento de los camarones ni en las tilapias. Sin embargo, los bloques experimentales presentaron diferencias significativas (P<0.05) en el peso promedio final. Los camarones de los bloques B y C fueron estadísticamente más grandes (P<0.05) que los del bloque A. Para las dos etapas de crecimiento de los camarones, no se observó un efecto de la presencia de tilapias sobre la sobrevivencia o crecimiento de los camarones.

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