scholarly journals Induced maturation of eel weighed 100–150 gram with PMSG, antidopamine, and 17α-methyltestosterone

2015 ◽  
Vol 14 (2) ◽  
pp. 135 ◽  
Author(s):  
Nadia Mega Aryani ◽  
Agus Oman Sudrajat ◽  
Odang Carman
Keyword(s):  
Gonadosomatic Index ◽  
Size Variation ◽  
Hormonal Treatment ◽  
Fish Length ◽  
Gonadal Maturation ◽  
Pregnant Mare Serum Gonadotropin ◽  
Gonadal Histology ◽  
Fish Weight ◽  
Serum Gonadotropin ◽  
Index Value

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">Marketed eel <em>Anguilla bicolor bicolor</em> is commonly produced from larvae rearing activity whose broodstocks and larvae are caught from the nature. Supply of eel broodstock is restricted by its life cycle and uncertain size variation of mature male and female. This study was aimed to evaluate the effect of hormonal induction through injection to enhance masculinization and gonadal maturation of eel at the weight of 100–150 g. The experiment used completely random design with the use of combination pregnant mare serum gonadotropin  (PMSG), antidopamine (AD), dan 17α-methyltestosterone (MT), which were (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), and P5 (control; without hormonal treatment). The result showed that an increasing of fish length along with fish weight were performed by treatment P4 and P3. The highest gonadosomatic index value was obtained by treatment P3 (1,3030±0,24262). Based on gonadal histology analysis, 2<sup>nd</sup> phase of spermatogonia development was found in P3 in week-8. The highest testosterone level was obtained by treatment P3, followed by P4, P2, and P1 in week-4. Combination of 10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT could enhance masculinization and gonadal maturation of eel in eight weeks of rearing period.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: gonadal maturation, <em>Anguilla bicolor bicolor</em>, PMSG, AD, MT</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">Ikan sidat <em>Anguilla bicolor bicolor</em> yang dipasarkan pada umumnya merupakan hasil usaha pembesaran yang benih dan induknya masih diperoleh dari alam. Penyediaan induk ikan sidat terkendala dengan siklus hidup dan variasi perbedaan ukuran induk ikan sidat jantan dan betina matang gonad yang belum pasti. Penelitian ini bertujuan untuk mengkaji peran induksi hormonal yang disuntikkan pada ikan sidat dalam mempercepat proses pematangan gonad ikan sidat ukuran 100–150 g. Metode penelitian yang digunakan adalah metode eksperimen rancangan acak lengkap dengan kombinasi <em>pregnant mare serum gonadotropin</em> (PMSG), antidopamin (AD), dan  17α-metiltestosteron (MT) sebagai berikut P1 (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), dan P5 (kontrol; tanpa perlakuan hormon). Hasil penelitian menunjukkan peningkatan pertambahan panjang seiring dengan pertambahan bobot diperoleh dari perlakuan P4 dan P3. Indeks gonadosomatik tertinggi diperoleh dari perlakuan P3 (1,3030±0,24262). Hasil histologi gonad ditemukan perkembangan spermatogonia fase 2 pada P3 di minggu kedelapan. Konsentrasi testosteron tertinggi didapat dari perlakuan P3 kemudian diikuti P4, P2, dan P1 pada minggu keempat. Kombinasi hormon 10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT dapat mempercepat pematangan gonad dan pertumbuhan pada ikan sidat selama delapan minggu pemeliharaan.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: pematangan gonad, <em>Anguilla bicolor bicolor</em>, PMSG, AD, MT</p><p> </p>

scholarly journals Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β

2015 ◽  
Vol 14 (2) ◽  
pp. 112
Author(s):  
Aprelia Martina Tomasoa ◽  
Agus Oman Sudrajat ◽  
Muhammad Zairin Junior
Keyword(s):  
Growth Hormone ◽  
Intramuscular Injection ◽  
Hormonal Treatment ◽  
Initial Body Weight ◽  
Gonadal Maturation ◽  
Combination Treatments ◽  
Pregnant Mare Serum Gonadotropin ◽  
Randomized Design ◽  
Serum Gonadotropin ◽  
Estradiol 17Β

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The study was aimed to induce gonadal maturation of eel <em>Anguilla bicolor bicolor</em> by hormonal treatment using <em>pregnant mare serum gonadotropin</em> (PMSG), antidopamine (AD), dan estradiol-17β (E2). The research used complete randomized design with five hormone combination treatments consisted of PK (NaCl 0.95%) as control, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), and P20BE (PMSG 20 IU + AD 10 ppm + E2 150 µg), with three individual replications for each treatment. Hormonal induction was applied through intramuscular injection weekly during eight weeks at initial body weight of 200 g. The result showed that P10BE treatment has obtained highest level on E2 (0.43 ng/mL), FSH (2.68 mIU/mL) has increased in week-4 and level on T (1.2 ng/mL), LH (2.80 mIU/mL) has increased in week-8. P10BE has affected spermatogenesis and the increased of GSI (2.46%) in fourth and sixth week compared to PK (1.28%), P10A (1.58%), P20A (1.34%), and P20BE (2.12%). In conclusion, combination of PMSG, AD, and E2 hormones could stimulate the gonadal maturation of eel at the size of 200 g into male.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Anguilla bicolor bicolor</em>, gonadal growth, hormone, maturation</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">Penelitian ini dilakukan untuk menginduksi pematangan gonad ikan sidat <em>Anguilla bicolor bicolor</em> secara hormonal dengan menggunakan <em>pregnant mare serum gonadothropin</em> (PMSG), antidopamin (AD), dan estradiol-17β (E2). Metode penelitian ini menggunakan metode eksperimen rancangan acak lengkap dengan lima perlakuan kombinasi hormon, yaitu PK (larutan NaCl 0,95%) sebagai kontrol, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), dan P20BE (PMSG 10 IU+AD 10 ppm+E2 150 µg), dengan tiga ulangan individu pada masing-masing perlakuan. Induksi hormonal dilakukan dengan metode penyutikan secara intramuskuler setiap minggu selama delapan minggu dengan bobot ikan yang berukuran 200 g. Hasil penelitian menunjukkan bahwa analisis hormon pada perlakuan P10BE memberikan nilai tertinggi masing-masing; kadar E2 (0,43 ng/mL), kadar FSH (2,68 mIU/mL) meningkat di minggu keempat dan kadar T (1,2 ng/mL), kadar LH (2,80 mIU/mL) mengalami peningkatan pada minggu kedelapan. P10BE memberikan efek pada spermatogenesis dan peningkatan nilai GSI (2,46%) pada minggu keempat sampai keenam selama penyuntikkan dibandingkan dengan PK (1,28%), P10A (1,58%), P20A (1,34%) dan P20BE (2,12%). Dengan demikian, kombinasi hormon PMSG, AD, dan E2 dapat merangsang perkembangan dan mempercepat pematangan gonad ikan sidat ukuran 200 g menjadi jantan.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: <em>Anguilla bicolor bicolor</em>, pertumbuhan gonad, hormon, maturasi</p>

scholarly journals Penggunaan serotonin dalam formulasi hormon pregnant mare serum gonadotropin dan antidopamin untuk menginduksi perkembangan gonad ikan ringau, Datnioides microlepis Bleeker, 1854 [Serotonin application in pregnant mare serum gonadotropin hormone and dopamin antagonist formulation to induce gonadal development of Indonesian tigerfish (Datnioides microlepis Bleeker, 1854)]

2017 ◽  
Vol 17 (1) ◽  
pp. 29
Author(s):  
NFN Bastiar ◽  
Agus Oman Sudrajat ◽  
Melta Rini Fahmi
Keyword(s):  
Plasma Concentration ◽  
Gonadosomatic Index ◽  
Gonad Development ◽  
Gonadal Development ◽  
Hepatosomatic Index ◽  
Small Fish ◽  
Pregnant Mare Serum Gonadotropin ◽  
Randomized Design ◽  
Serum Gonadotropin ◽  
Completely Randomized Design

The objective of study was to analyze the effect of serotonin (5-HT) in the formulation of hormones Pregnant Mare Serum Gonadotropin (PMSG) and dopamin antagonist (AD) hormones to gonad development of tigerfish. This study used completely randomized design (CRD) with five treatments of hormone namely: (P1) 1 ml of 0.9% NaCl (control); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG AD+10 mg+0.2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; and (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Each treatment was tested on five fish as individual replications. Hormone injected intramuscularly at the lower part of the dorsal fin of fish every 10 days. The fish were reared for 60 days. Fish that were used at this study were originate from natural catches as much as 25 fishes with 17.5-33.0 cm of total length and 118-926 g of body weight. During the study, fish fed using shrimp and small fish (live) twice daily at satiation. Measured parameters were gonadosomatic index (GSI), hepatosomatic index (HSI), 17P-estradiol (E2) plasma concentration and gonad maturity level based on morphology and histology examination. The results showed that the use of 2 mg of 5-HT are added to 20 IU PMSG and 10 mg AD (treatment P4) has stimulated the fish to had the highest GSI (2.38 ± 0.06%) and HSI (3,09±0,12%) which was significantly different to other treatment. The treatment (P4) could increase the E2 plasma concentration (37.14±2.99 pg.ml-1) two fold compared with the concentration before injection and stimulated the gonadal development to stage III. AbstrakPenelitian ini bertujuan untuk menganalisis pengaruh penggunaan serotonin (5- hydroxytryptamine atau 5-HT) dalam formulasi hormon Pregnant Mare Serum Gonadotropin (PMSG) dan antidopamin (AD) terhadap perkembangan gonad ikan ringau. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan (dosis.kg-1 bobot tubuh ikan): (P1) 1 ml NaCl 0,9% (kontrol); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG+10 mg AD+0,2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; dan (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Setiap perlakuan diujikan pada lima ekor ikan sebagai ulangan individu. Penyuntikan hormon dilakukan setiap 10 hari dengan lama penelitian 60 hari. Hormon disuntikkan secara intramuskular pada bagian bawah sirip punggung ikan uji. Ikan yang digunakan merupakan hasil tangkapan alam dengan ukuran panjang total 17,5-33,0 cm dan bobot tubuh 118-926 g. Selama penelitian, ikan uji diberi pakan berupa udang dan ikan-ikan kecil (hidup) dua kali sehari secara satiasi. Parameter yang diamati adalah indeks kematangan gonad, indeks hepatosomatik, konsentrasi estradiol-17p plasma dan tingkat kematangan gonad berdasarkan morfologi dan histologi gonad. Hasil penelitian menunjukkan penggunaan 2 mg 5-HT dalam 20 IU PMSG dan 10 mg AD (perlakuan P4) menghasilkan perkembangan gonad yang lebih baik dibandingkan perlakuan lainnya. Nilai indeks kematangan gonad, indeks hepatosomatik, dan konsentrasi E2 plasma tertinggi diperoleh pada perlakuan P4 dengan nilai masing-masing sebesar 2,38±0,06%; 3,09±0,12% dan 37,14±2,99 pg.ml-1. Nilai tersebut berbeda nyata p<0.05) dengan perlakuan lainnya. Perlakuan P4 meningkatkan konsentrasi E2 plasma dua kali lebih besar dibanding-kan sebelum penyuntikan serta menghasilkan perkembangan gonad yang mencapai tingkat kematangan gonad tahap III.

scholarly journals Reproductive Biology of Splendid Ponyfish Leiognathus splendens (Cuvier, 1829) in Myeik Coastal Waters, Myanmar

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Khin May Chit Maung ◽  
Phan Minh-Thu ◽  
Nyo Nyo Tun
Keyword(s):  
Reproductive Biology ◽  
Coastal Waters ◽  
Gonadosomatic Index ◽  
Fish Length ◽  
Gonad Weight ◽  
Fish Weight ◽  
The Mean ◽  
First Maturity ◽  
Relationship Of ◽  
Trawl Fisheries

Reproductive biology of Leiognathus splendens was studied by using the samples collected from the catches of trawl fisheries in Myeik coastal waters during January to December 2014. Spawning takes place throughout the year, with a peak during April-May and September-December. The observed length at first maturity was 9.0 cm total length in males and 8.4 cm in females. The mean gonadosomatic index was more prominently in females (2.5) than males (1.5). Sex ratio (1 male: 1.1 females) also indicates the general dominant of females over males  Fecundity varied from 6120 to 58412 eggs. Relationship of fecundity to fish length, fish weight and ovary weight showed that fecundity is more related to the gonad weight (r=0.9) than length (r=0.8) and weight of fish (r=0.7)

scholarly journals Pengaruh Hormon Pregnan Mare Serum (PMSG) Murni dan Kombinasi terhadap Gonadosomatik Indeks, Hepatosomatik Indeks Ikan Bawal Bintang (Trachinotus blochii)

2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Wiwin Kusuma Atmaja Putra ◽  
Tengku Said Razai
Keyword(s):  
Body Weight ◽  
Gonadosomatic Index ◽  
Hormone Treatment ◽  
Hepatosomatic Index ◽  
Pregnant Mare Serum Gonadotropin ◽  
Randomized Design ◽  
Absolute Growth ◽  
Serum Gonadotropin ◽  
Completely Randomized Design ◽  
Trachinotus Blochii

The research was title The Effect of Pregnant Mare Serum Gonadotropin (PMSG) Pure and Combinations against Gonadosomatik Index, Hepatosomatic Index Silver Pompano (Trachinotus blochii). The purpose of this research was to obtain the best hormone treatment to increase the gonadosomatic index and Hepatosomatic Index. This research used a Completely Randomized Design with 3 treatments and 3 replicates of fish. The treatments include Control (0.5 ml NaCl), PMSG (20 IU kg-1 fish body weight) and PG600 (20 IU. Kg-1 fish body weight). Parameters calculated include Gonadosomatic Index (GSI), Hepatosomatic Index (HSI) and Absolute Growth. The best results were PG600 treatment with Gonadosomatic Index (0.12±0.06), Hepatosomatic Index (2.29±0.15 %) and Absolute Growth    (85±31.32 g) for 4 weeks. The conclusion of this study is the provision of pure PMSG and combination with a dose of      20 IU. Kg-1 body weight can  increase gonadosomatik index, hepatosomatic index and absolute growth of Silver Pompano fish. Keywords : Silver Pompano Fish, PMSG, PG600, Gonadosomatic Index

scholarly journals PERTUMBUHAN DAN PEMATANGAN GONAD IKAN GIRU Amphiprion clarkii YANG DIBERI PAKAN MENGANDUNG HORMON OODEV

2019 ◽  
Vol 9 (2) ◽  
pp. 163-168
Author(s):  
Aprelia Martina Tomasoa ◽  
Deidy Azhari ◽  
Walter Balansa
Keyword(s):  
Gonadosomatic Index ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin ◽  
Amphiprion Clarkii

Pertumbuhan dan pematangan gonad memainkan peranan penting dalam budidaya ikan. Untuk memperoleh benih yang berkualitas, induk harus benar benar matang gonad dan memiliki ukuran tubuh yang sesuai. Umum digunakan dalam kegiatan budidaya ikan, hormon Oodev dapat merangsang pematangan gonad karena hormon Oodev ini mengandung pregnant mare serum gonadotropin dan antidopamin yang sama-sama berperan dalam pematangan gonad. Sekalipun hormon ini begitu populer di bidang budidaya ikan, pengaruh hormon ini terhadap pertumbuhan tubuh dan pematangan gonad terhadap ikan giru (Amphiprion clarkii) belum banyak diketahui. Penelitian ini bertujuan untuk mengevaluasi pengaruh dan menentukan dosis pemberian hormon Oodev dalam pakan terhadap pertumbuhan panjang dan bobot serta pematangan gonad ikan giru. Ikan giru diberi perlakuan dengan tiga dosis hormon Oodev berbeda yaitu 0 mL/kg, 0,5 mL/kg, dan 1 mL/kg dengan tiga pengulangan selama 30 hari pemeliharaan. Hasil menunjukkan dosis 1 mL/kg meningkatkan pertumbuhan panjang tubuh pada jantan (0,9 cm) maupun betina (0,7 cm) dan pertumbuhan bobot pada jantan (1,32 gram) maupun betina (3,05 gram) lebih tinggi dengan kontrol. Sejalan dengan pertumbuhan panjang dan bobot, dosis 1 mL/kg meningkatkan nilai gonadosomatic index jantan dan betina yaitu 0,47% dan 0,58% lebih tinggi dengan kontrol. Dari hasil yang diperoleh dapat ditarik kesimpulan bahwa hormon Oodev dengan dosis 1 mL/kg dapat meningkatkan pertumbuhan dan nilai GSI A. clarkii.

scholarly journals Reproductive characteristics of Liza parsia (Ham.) inhabiting southwest coast of Bangladesh

1970 ◽  
Vol 8 (1) ◽  
pp. 173-178 ◽  
Author(s):  
M Begum ◽  
MA Islam ◽  
HK Pal ◽  
MJ Alam
Keyword(s):  
Sex Ratio ◽  
Gonadosomatic Index ◽  
Fish Length ◽  
Gonad Weight ◽  
Reproductive Characteristics ◽  
Linear Relationships ◽  
Fish Weight ◽  
Reproduction Period ◽  
Two Peaks ◽  
Egg Diameter

In order to determine the reproductive characteristics of Liza parsia, a fish of commercial importance, the GSI, egg diameter, sex ratio and fecundity were examined in 331 specimens caught monthly for a period of ten months from September 2006 to June 2007. According to the GSI and egg diameter, the reproduction period of Liza parsia was determined to be in November to March with two peaks in the months of December and February. The maximum GSI values obtained for male and female were 1.49 and 14.71 respectively in the month of February. In December, the gonadosomatic index decreased in female (13.5) and in male (1.4). The average egg diameter of Liza parsia ranged from 0.35 to 0.50 mm. The sex ratio (Male:Female) of the investigated fish was1:1.21. Fecundity of the fish ranged from 18,950 to 1,71,210 during the period of study. Linear relationships between fecundity and total-length, bodyweight, gonad-length and gonad-weight were found and fecundity was more related to the ovary length (r=0.8172) and ovary weight (r=0.8552) than the fish length (r=0.7538) and fish weight (r=0.7592). Keywords: Reproductive characteristics; Liza parsia; GSI; Fecundity DOI: 10.3329/jbau.v8i1.6415J. Bangladesh Agril. Univ. 8(1): 173-178, 2010

scholarly journals INDUKSI PEMATANGAN GONAD IKAN GURAMI COKELAT (Sphaerichthys osphromenoides Canestrini, 1860) MENGGUNAKAN PREGNANT MARE SERUM GONADOTROPIN DAN ANTIDOPAMIN

2017 ◽  
Vol 12 (1) ◽  
pp. 69
Author(s):  
Bastiar Nur ◽  
Sawung Cindelaras ◽  
Nina Meilisza
Keyword(s):  
Body Weight ◽  
Fish Species ◽  
Gonadosomatic Index ◽  
Gonad Development ◽  
Stage Iv ◽  
Reproductive Activity ◽  
Gonadal Development ◽  
Pregnant Mare Serum Gonadotropin ◽  
Gonad Somatic Index ◽  
Serum Gonadotropin

Ikan gurami cokelat (Sphaerichthys osphromenoides Canestrini, 1860) merupakan salah satu spesies ikan hias endemik perairan gambut dan memiliki potensi untuk dibudidayakan. Pada kondisi budidaya, berbagai faktor lingkungan yang berperan penting dalam menstimulasi perkembangan dan pematangan gonad hingga ovulasi dan pemijahan tidak mendukung aktivitas reproduksi beberapa spesies ikan. Penelitian ini dilakukan untuk menentukan dosis hormon yang efektif dapat merangsang pematangan gonad ikan gurami cokelat. Hormon yang digunakan adalah “Oodev®” (tersusun atas Pregnant Mare Serum Gonadotropin/PMSG) dan antidopamin) yang diberikan menggunakan metode “topical gill”. Ada tiga dosis Oodev® yang digunakan, yaitu: 0,02 mL; 0,04 mL; dan 0,06 mL; serta kontrol menggunakan NaCl 0,9% sebanyak 0,05 mL/g bobot badan ikan uji. Setiap perlakuan menggunakan 30 ekor induk betina ikan gurami cokelat (panjang total: 4,1 ± 0,3 cm; bobot: 1,41 ± 0,17 g). Pemberian hormon dilakukan setiap minggu hingga minggu ke-7. Pada minggu ke-8 dilakukan pembedahan untuk pengambilan gonad. Parameter yang diamati adalah: jumlah induk matang gonad, indeks gonadosomatik (IGS), fekunditas, diameter oosit, kadar estradiol-17â dalam darah, dan tingkat kematangan gonad (TKG) ikan uji pada masing-masing perlakuan. Hasil penelitian menunjukkan bahwa perlakuan hormon dengan dosis 0,04 mL/g bobot badan menghasilkan perkembangan gonad ikan uji yang lebih baik dengan jumlah induk matang gonad mencapai 23 ekor (76,67%), nilai IGS sebesar 2,33 ± 1,24%; fekunditas sebesar 73,5 ± 26,2 butir; diameter oosit berkisar antara 1,0-1,8 mm; kadar estradiol-17b dalam darah sebesar 15,9 ± 4,5 rg/mL dengan tingkat kematangan gonad mencapai tahap IV.Chocolate gourami (Sphaerichthys osphromenoides Canestrini, 1860) is an endemic ornamental fish species in peatland waters and potentially to be cultivated. In captive condition, some environmental factors that play important role for gonadal development, maturation, ovulation, and spawning are not suitable for supporting reproductive activity in some fish species. This study was conducted to determine the optimum dosages of hormone which is able to stimulate gonadal maturation of chocolate gourami. Oodev® (consisted of Pregnant Mare Serum Gonadotropin (PMSG) and antidopamin) was given using “topical gill” method. There were three Oodev® dosages used in this research: 0.02 mL; 0.04 mL; and 0.06 mL respectively; and control using 0.05 mL of 0.9% NaCl per gram of body weight. Each treatment was tested on 30 females (the averages of total length and body weight of 4.1 ± 0.3 cm and 1.41 ± 0.17 g, respectively). Hormone was given every week until the seventh week. At the eight weeks, surgery was performed for gonadal measurement. Parameters measured were: number of gonadal mature broodstocks, gonadosomatic index, fecundity, oocyte diameters, plasma estradiol-17â levels, and gonadal mature levels. The results showed that 0.04 mL Oodev® per g body weight of fish was superior in reproductive performance. In that treatment, there were 76.67% (23/30) fish being matured, gonad somatic index 2.33 ± 1.24%, egg fecundity 73.5 ± 26.2 eggs, oocyte diameters ranged 1.0-1.8 mm, blood level of estradiol-17b 15.9 ± 4.5 rg.mL-1, and the level of gonad development reached to stage IV.

scholarly journals Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish

2015 ◽  
Vol 14 (1) ◽  
pp. 24 ◽  
Author(s):  
Rizal Akbar Hutagalung ◽  
Maheno Sri Widodo ◽  
Abdul Rahem Faqih
Keyword(s):  
Gonadosomatic Index ◽  
Follicle Stimulating Hormone ◽  
Economic Value ◽  
Female Parent ◽  
Hepatosomatic Index ◽  
Female Fish ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin ◽  
Channa Striata ◽  
Different Doses

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Snakehead fish (<em>Channa striata</em>) is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG) and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH) which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI) and hepatosomatic index (HSI). The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: PMSG, GSI, HSI, snakehead fish</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Ikan gabus (<em>Channa striata</em>) merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah <em>pregnant mare serum gonadotropin</em> (PMSG) dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur <em>follicle stimulating hormon </em>(FSH) yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS) dan indeks hepatosomatik (IHS). Induk ikan gabus yang digunakan berukuran 30−40 cm dengan bobot 500–700 g, diaklimatisasikan di akuarium kemudian diinduksi hormon PMSG dengan perlakuan dosis A: 0,75 mL/kg; B: 1,0 mL/kg; C: 1,25 mL/kg; D: 1,5 mL/kg dan dilakukan pengulangan sebanyak tiga kali. Selanjutnya ditunggu hingga 72 jam untuk mengevaluasi perkembangan IGS dan IHS nya. Hasil penelitian menunjukkan bahwa perlakuan terbaik adalah dosis 1,25 ml/kg dengan nilai rata-rata IHS 1,37% dan IGS 3,35%,</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: PMSG, IGS, IHS, ikan gabus</p><p> </p>

scholarly journals Reproductive Biology of Splendid Ponyfish Leiognathus Splendens (Cuvier, 1829) in Myeik Coastal Waters, Myanmar

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Khin May Chit Maung ◽  
Phan Minh-Thu ◽  
Nyo Nyo Tun
Keyword(s):  
Reproductive Biology ◽  
Coastal Waters ◽  
Gonadosomatic Index ◽  
Fish Length ◽  
Gonad Weight ◽  
Fish Weight ◽  
The Mean ◽  
First Maturity ◽  
Relationship Of ◽  
Trawl Fisheries

Reproductive biology of Leiognathus splendens was studied by using the samples collected from the catches of trawl fisheries in Myeik coastal waters during January to December 2014. Spawning takes place throughout the year, with a peak during April-May and September-December. The observed length at first maturity was 9.0 cm total length in males and 8.4 cm in females. The mean gonadosomatic index was more prominently in females (2.5) than males (1.5). Sex ratio (1 male: 1.1 females) also indicates the general dominant of females over males  Fecundity varied from 6120 to 58412 eggs. Relationship of fecundity to fish length, fish weight and ovary weight showed that fecundity is more related to the gonad weight (r=0.9) than length (r=0.8) and weight of fish (r=0.7)

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