Penggunaan serotonin dalam formulasi hormon pregnant mare serum gonadotropin dan antidopamin untuk menginduksi perkembangan gonad ikan ringau, Datnioides microlepis Bleeker, 1854  [Serotonin application in pregnant mare serum gonadotropin hormone and dopamin antagonist formulation to induce gonadal development of Indonesian tigerfish (Datnioides microlepis  Bleeker, 1854)]

The objective of study was to analyze the effect of serotonin (5-HT) in the formulation of hormones Pregnant Mare Serum Gonadotropin (PMSG) and dopamin antagonist (AD) hormones to gonad development of tigerfish. This study used completely randomized design (CRD) with five treatments of hormone namely: (P1) 1 ml of 0.9% NaCl (control); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG AD+10 mg+0.2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; and (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Each treatment was tested on five fish as individual replications. Hormone injected intramuscularly at the lower part of the dorsal fin of fish every 10 days. The fish were reared for 60 days. Fish that were used at this study were originate from natural catches as much as 25 fishes with 17.5-33.0 cm of total length and 118-926 g of body weight. During the study, fish fed using shrimp and small fish (live) twice daily at satiation. Measured parameters were gonadosomatic index (GSI), hepatosomatic index (HSI), 17P-estradiol (E2) plasma concentration and gonad maturity level based on morphology and histology examination. The results showed that the use of 2 mg of 5-HT are added to 20 IU PMSG and 10 mg AD (treatment P4) has stimulated the fish to had the highest GSI (2.38 ± 0.06%) and HSI (3,09±0,12%) which was significantly different to other treatment. The treatment (P4) could increase the E2 plasma concentration (37.14±2.99 pg.ml-1) two fold compared with the concentration before injection and stimulated the gonadal development to stage III. AbstrakPenelitian ini bertujuan untuk menganalisis pengaruh penggunaan serotonin (5- hydroxytryptamine atau 5-HT) dalam formulasi hormon Pregnant Mare Serum Gonadotropin (PMSG) dan antidopamin (AD) terhadap perkembangan gonad ikan ringau. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan (dosis.kg-1 bobot tubuh ikan): (P1) 1 ml NaCl 0,9% (kontrol); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG+10 mg AD+0,2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; dan (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Setiap perlakuan diujikan pada lima ekor ikan sebagai ulangan individu. Penyuntikan hormon dilakukan setiap 10 hari dengan lama penelitian 60 hari. Hormon disuntikkan secara intramuskular pada bagian bawah sirip punggung ikan uji. Ikan yang digunakan merupakan hasil tangkapan alam dengan ukuran panjang total 17,5-33,0 cm dan bobot tubuh 118-926 g. Selama penelitian, ikan uji diberi pakan berupa udang dan ikan-ikan kecil (hidup) dua kali sehari secara satiasi. Parameter yang diamati adalah indeks kematangan gonad, indeks hepatosomatik, konsentrasi estradiol-17p plasma dan tingkat kematangan gonad berdasarkan morfologi dan histologi gonad. Hasil penelitian menunjukkan penggunaan 2 mg 5-HT dalam 20 IU PMSG dan 10 mg AD (perlakuan P4) menghasilkan perkembangan gonad yang lebih baik dibandingkan perlakuan lainnya. Nilai indeks kematangan gonad, indeks hepatosomatik, dan konsentrasi E2 plasma tertinggi diperoleh pada perlakuan P4 dengan nilai masing-masing sebesar 2,38±0,06%; 3,09±0,12% dan 37,14±2,99 pg.ml-1. Nilai tersebut berbeda nyata p<0.05) dengan perlakuan lainnya. Perlakuan P4 meningkatkan konsentrasi E2 plasma dua kali lebih besar dibanding-kan sebelum penyuntikan serta menghasilkan perkembangan gonad yang mencapai tingkat kematangan gonad tahap III.

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Pengaruh Hormon Pregnan Mare Serum (PMSG) Murni dan Kombinasi terhadap Gonadosomatik Indeks, Hepatosomatik Indeks Ikan Bawal Bintang (Trachinotus blochii)

Journal of Aquaculture Science ◽

10.31093/joas.v2i1.14 ◽

2017 ◽

Vol 2 (1) ◽

Author(s):

Wiwin Kusuma Atmaja Putra ◽

Tengku Said Razai

Keyword(s):

Body Weight ◽

Gonadosomatic Index ◽

Hormone Treatment ◽

Hepatosomatic Index ◽

Pregnant Mare Serum Gonadotropin ◽

Randomized Design ◽

Absolute Growth ◽

Serum Gonadotropin ◽

Completely Randomized Design ◽

Trachinotus Blochii

The research was title The Effect of Pregnant Mare Serum Gonadotropin (PMSG) Pure and Combinations against Gonadosomatik Index, Hepatosomatic Index Silver Pompano (Trachinotus blochii). The purpose of this research was to obtain the best hormone treatment to increase the gonadosomatic index and Hepatosomatic Index. This research used a Completely Randomized Design with 3 treatments and 3 replicates of fish. The treatments include Control (0.5 ml NaCl), PMSG (20 IU kg-1 fish body weight) and PG600 (20 IU. Kg-1 fish body weight). Parameters calculated include Gonadosomatic Index (GSI), Hepatosomatic Index (HSI) and Absolute Growth. The best results were PG600 treatment with Gonadosomatic Index (0.12±0.06), Hepatosomatic Index (2.29±0.15 %) and Absolute Growth (85±31.32 g) for 4 weeks. The conclusion of this study is the provision of pure PMSG and combination with a dose of 20 IU. Kg-1 body weight can increase gonadosomatik index, hepatosomatic index and absolute growth of Silver Pompano fish. Keywords : Silver Pompano Fish, PMSG, PG600, Gonadosomatic Index

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INDUKSI PEMATANGAN GONAD IKAN GURAMI COKELAT (Sphaerichthys osphromenoides Canestrini, 1860) MENGGUNAKAN PREGNANT MARE SERUM GONADOTROPIN DAN ANTIDOPAMIN

Jurnal Riset Akuakultur ◽

10.15578/jra.12.1.2017.69-76 ◽

2017 ◽

Vol 12 (1) ◽

pp. 69

Author(s):

Bastiar Nur ◽

Sawung Cindelaras ◽

Nina Meilisza

Keyword(s):

Body Weight ◽

Fish Species ◽

Gonadosomatic Index ◽

Gonad Development ◽

Stage Iv ◽

Reproductive Activity ◽

Gonadal Development ◽

Pregnant Mare Serum Gonadotropin ◽

Gonad Somatic Index ◽

Serum Gonadotropin

Ikan gurami cokelat (Sphaerichthys osphromenoides Canestrini, 1860) merupakan salah satu spesies ikan hias endemik perairan gambut dan memiliki potensi untuk dibudidayakan. Pada kondisi budidaya, berbagai faktor lingkungan yang berperan penting dalam menstimulasi perkembangan dan pematangan gonad hingga ovulasi dan pemijahan tidak mendukung aktivitas reproduksi beberapa spesies ikan. Penelitian ini dilakukan untuk menentukan dosis hormon yang efektif dapat merangsang pematangan gonad ikan gurami cokelat. Hormon yang digunakan adalah “Oodev®” (tersusun atas Pregnant Mare Serum Gonadotropin/PMSG) dan antidopamin) yang diberikan menggunakan metode “topical gill”. Ada tiga dosis Oodev® yang digunakan, yaitu: 0,02 mL; 0,04 mL; dan 0,06 mL; serta kontrol menggunakan NaCl 0,9% sebanyak 0,05 mL/g bobot badan ikan uji. Setiap perlakuan menggunakan 30 ekor induk betina ikan gurami cokelat (panjang total: 4,1 ± 0,3 cm; bobot: 1,41 ± 0,17 g). Pemberian hormon dilakukan setiap minggu hingga minggu ke-7. Pada minggu ke-8 dilakukan pembedahan untuk pengambilan gonad. Parameter yang diamati adalah: jumlah induk matang gonad, indeks gonadosomatik (IGS), fekunditas, diameter oosit, kadar estradiol-17â dalam darah, dan tingkat kematangan gonad (TKG) ikan uji pada masing-masing perlakuan. Hasil penelitian menunjukkan bahwa perlakuan hormon dengan dosis 0,04 mL/g bobot badan menghasilkan perkembangan gonad ikan uji yang lebih baik dengan jumlah induk matang gonad mencapai 23 ekor (76,67%), nilai IGS sebesar 2,33 ± 1,24%; fekunditas sebesar 73,5 ± 26,2 butir; diameter oosit berkisar antara 1,0-1,8 mm; kadar estradiol-17b dalam darah sebesar 15,9 ± 4,5 rg/mL dengan tingkat kematangan gonad mencapai tahap IV.Chocolate gourami (Sphaerichthys osphromenoides Canestrini, 1860) is an endemic ornamental fish species in peatland waters and potentially to be cultivated. In captive condition, some environmental factors that play important role for gonadal development, maturation, ovulation, and spawning are not suitable for supporting reproductive activity in some fish species. This study was conducted to determine the optimum dosages of hormone which is able to stimulate gonadal maturation of chocolate gourami. Oodev® (consisted of Pregnant Mare Serum Gonadotropin (PMSG) and antidopamin) was given using “topical gill” method. There were three Oodev® dosages used in this research: 0.02 mL; 0.04 mL; and 0.06 mL respectively; and control using 0.05 mL of 0.9% NaCl per gram of body weight. Each treatment was tested on 30 females (the averages of total length and body weight of 4.1 ± 0.3 cm and 1.41 ± 0.17 g, respectively). Hormone was given every week until the seventh week. At the eight weeks, surgery was performed for gonadal measurement. Parameters measured were: number of gonadal mature broodstocks, gonadosomatic index, fecundity, oocyte diameters, plasma estradiol-17â levels, and gonadal mature levels. The results showed that 0.04 mL Oodev® per g body weight of fish was superior in reproductive performance. In that treatment, there were 76.67% (23/30) fish being matured, gonad somatic index 2.33 ± 1.24%, egg fecundity 73.5 ± 26.2 eggs, oocyte diameters ranged 1.0-1.8 mm, blood level of estradiol-17b 15.9 ± 4.5 rg.mL-1, and the level of gonad development reached to stage IV.

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Plasma levels of luteinizing hormone and gonadal maturation of lambari females under different photoperiods

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2013000800036 ◽

2013 ◽

Vol 48 (8) ◽

pp. 1064-1070 ◽

Cited By ~ 1

Author(s):

Fernanda Keley Silva Pereira Navarro ◽

Rodrigo Diana Navarro ◽

Luis David Solis Murgas ◽

Marina Apocalypse Nogueira Pereira ◽

Guilherme Crispim Hundley

Keyword(s):

Luteinizing Hormone ◽

Plasma Levels ◽

Gonadosomatic Index ◽

Hepatosomatic Index ◽

Ovarian Maturation ◽

Gonadal Maturation ◽

Ovary Weight ◽

Randomized Design ◽

Final Weight ◽

Completely Randomized Design

The objective of this work was to evaluate the effect of photoperiod on luteinizing hormone (LH) plasma levels and gonadal maturation of lambari females. One hundred and twenty adult lambaris, distributed into 12 aquaria of 20 L each, were randomly subjected to three different treatments, in a completely randomized design, and four replicates. Treatments were photoperiods in hours of light (L) and darkness (D): T1, 0 L:24 D; T2, 12 L:12 D; T3, 24 L:0 D. After 40 days, fish were subjected to fasting for 24 hours and, then, anesthetized. Immediately after slaughter, fish were weighed, and their gonads and livers were removed and weighed. Ovaries were weighed and immersed in Bouin's fixative solution for 24 hours and, then, kept in 70% alcohol until processing the material. Subsequently, the oocyte developmental stage was determined. No significant differences were observed between treatments for final weight, ovary weight, gonadosomatic index (GSI), hepatosomatic index (HSI) and LH levels. In all treatments, lambari females showed maturing ovaries with prevalence of vitellogenic oocytes. Photoperiod does not affect the LH levels and ovarian maturation in lambari females.

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Hormonal induction maturation of silver pompano Trachinotus blochii

Jurnal Akuakultur Indonesia ◽

10.19027/jai.19.1.61-73 ◽

2020 ◽

Vol 19 (1) ◽

pp. 61-73

Author(s):

Wiwin Kusuma Atmaja Putra ◽

Tengku Said Raza’i

Keyword(s):

Body Weight ◽

Gonadosomatic Index ◽

Hormone Treatment ◽

Hepatosomatic Index ◽

Gonad Maturation ◽

Maturity Level ◽

Randomized Design ◽

Absolute Growth ◽

Completely Randomized Design ◽

Trachinotus Blochii

ABSTRACT This study was aimed to acquire the most suitable hormone treatment to induce gonad maturation of silver pompano Trachinotus blochii. This study used a completely randomized design with four treatments and three replications, i.e NaCl 90% (control) (0.5 mL/kg), hCG (20 IU/kg fish body weight), PMSG (20 IU/kg fish body weight), and PG600 (20 IU/kg fish body weight). Parameters measured were gonadosomatic index (GSI), hepatosomatic index (HSI), gonad maturity level (TKG), gonad histology, and absolute growth. The best result was PG600 treatment with 0.12% gonadosomatic index, 2.29% hepatosomatic index, TKG IV, and 85 g absolute growth for four weeks. This study concludes that the most suitable hormone to induce gonad maturation of silver pompano Trachinotus blochii is PG600 with 20 IU/kg body weight, which effectively affects GSI, HSI, and absolute growth of silver pompano. Keywords: hCG, maturation, PG600, PMSG, silver pompano. ABSTRAK Penelitian ini bertujuan untuk mendapatkan hormon yang terbaik untuk menginduksi maturasi gonadikan bawal bintang. Hormon yang digunakan pada penelitian ini adalahkontrol NaCl 90% (dosis 0,5 mL/kg), hCG(dosis 20 IU/kg bobot tubuh ikan), PMSG (dosis 20 IU/kg bobot tubuh ikan), dan PG600 (dosis 20 IU/kg bobot tubuh ikan). Parameter yang diamati adalah indeks gonadosomatik (GSI), indeks hepatosomatik (HSI), tingkat kematangan gonad (TKG), histologi gonad, dan pertumbuhan bobot mutlak. Hasil terbaik adalah perlakuan PG600 dengan gonadosomatik indeks (0,12%), hepatosomatik indeks (2,29%), TKG IV,dan pertumbuhan mutlak (85 g) selama empat minggu. Kesimpulan penelitian ini adalah perlakuan hormone yang berpotensi menginduksi maturasi gonad ikan bawal bintang adalah hormon PG600 dengan dosis 20 IU/kg bobot tubuh dikarenakan adanya pengaruh nyata tehadap parameter GSI, HSI dan pertumbuhan mutlak ikan bawal bintang. Kata kunci: ikan bawal bintang, hCG, PMSG, PG600, maturasi.

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Effect of electrical field on gonadal development of goldfish in saline media

Jurnal Akuakultur Indonesia ◽

10.19027/jai.14.9-17 ◽

2015 ◽

Vol 14 (1) ◽

pp. 9

Author(s):

Kukuh Nirmala ◽

Ahmad Habibie ◽

Harton Arfah

Keyword(s):

Body Weight ◽

Carassius Auratus ◽

Gonadosomatic Index ◽

Gonadal Development ◽

Electrical Field ◽

Field Exposure ◽

Randomized Design ◽

Completely Randomized Design ◽

Average Body ◽

Average Body Weight

ABSTRACT The aim of this study was to evaluate the effect of electric field exposure duration at the voltage of 10 volt on goldfish Carassius auratus auratus gonadal development maintained in 3 ppt salinity media. The experiment consisted of four treatments in triplicates i.e. control, two, four, and six minutes of electrical-field exposure. The experiment design used was completely randomized design. Fish used was female goldfish at the density of 4 fish/aquarium with an average total length of 12.27±0.05 cm and average body weight of 22.29±0.54 g. Result of study showed that the electrical-field exposure at 10 volt for all duration treatments in 3 ppt of media salinity did not give significant effect on gonadosomatic index (GSI) and gonadal development of goldfish. Keywords: electrical field, Carassius auratus auratus, gonad, salinity ABSTRAK Tujuan penelitian ini adalah untuk mengevaluasi pengaruh lama waktu pemaparan medan listrik dengan voltase 10 volt terhadap perkembangan gonad ikan komet Carassius auratus auratus yang dipelihara pada media bersalinitas 3 ppt. Perlakuan pada penelitian ini terdiri atas empat perlakuan, yaitu: perlakuan kontrol, dua, empat, dan enam menit. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Ikan uji yang digunakan adalah ikan komet betina. Jumlah ikan yang digunakan adalah 4 ekor/akuarium dengan panjang total rata-rata 12,27±0,05 cm dan bobot tubuh rata-rata 22,29±0,54 g. Hasil penelitian menunjukkan bahwa pemberian medan listrik sebesar 10 volt dengan lama waktu pemaparan medan listrik pada semua perlakuan durasi di media pemeliharaan bersalinitas 3 ppt tidak memberikan pengaruh yang nyata terhadap parameter indeks gonadosomatik (GSI) dan perkembangan gonad ikan komet. Kata kunci: medan listrik, Carassius auratus auratus, gonad, salinitas

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Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish

Jurnal Akuakultur Indonesia ◽

10.19027/jai.14.24-29 ◽

2015 ◽

Vol 14 (1) ◽

pp. 24 ◽

Cited By ~ 1

Author(s):

Rizal Akbar Hutagalung ◽

Maheno Sri Widodo ◽

Abdul Rahem Faqih

Keyword(s):

Gonadosomatic Index ◽

Follicle Stimulating Hormone ◽

Economic Value ◽

Female Parent ◽

Hepatosomatic Index ◽

Female Fish ◽

Pregnant Mare Serum Gonadotropin ◽

Serum Gonadotropin ◽

Channa Striata ◽

Different Doses

ABSTRACT Snakehead fish (Channa striata) is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG) and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH) which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI) and hepatosomatic index (HSI). The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively. Keywords: PMSG, GSI, HSI, snakehead fish ABSTRAK Ikan gabus (Channa striata) merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah pregnant mare serum gonadotropin (PMSG) dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur follicle stimulating hormon (FSH) yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS) dan indeks hepatosomatik (IHS). Induk ikan gabus yang digunakan berukuran 30−40 cm dengan bobot 500–700 g, diaklimatisasikan di akuarium kemudian diinduksi hormon PMSG dengan perlakuan dosis A: 0,75 mL/kg; B: 1,0 mL/kg; C: 1,25 mL/kg; D: 1,5 mL/kg dan dilakukan pengulangan sebanyak tiga kali. Selanjutnya ditunggu hingga 72 jam untuk mengevaluasi perkembangan IGS dan IHS nya. Hasil penelitian menunjukkan bahwa perlakuan terbaik adalah dosis 1,25 ml/kg dengan nilai rata-rata IHS 1,37% dan IGS 3,35%, Kata kunci: PMSG, IGS, IHS, ikan gabus

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The Effect of Vitamin E Supplement in the Diet on Gonad Maturation of Nilem (Osteochilus hasselti, CV)

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.26509 ◽

2017 ◽

Vol 19 (1) ◽

pp. 1

Author(s):

Nurbety Tarigan ◽

Iman Supriatna ◽

M. Agus Setiadi ◽

Ridwan Affandi

Keyword(s):

Vitamin E ◽

Gonadosomatic Index ◽

Critical Role ◽

Gonad Development ◽

Feed Additives ◽

Hepatosomatic Index ◽

Gonad Maturation ◽

Fish Reproduction ◽

Gonad Maturity

The aim of this study was to examine the effect of vitamin E on gonad maturation and determining the best doses in improving gonad maturation in nilem fish. In this study used a flockof fishbrood which is never experienced spawning. There are 4 doses of vitamin E which used in this research following by 0, 125, 250, and 375 mg/kg. The results showed that fortifiedof vitamin E which were in feed significantly affected to the percentage of gonad development, gonadosomatic index (GSI), hepatosomatic index (IHS), fecundity, the diameter of eggs, and the percentage of egg maturity at P<0.05. The result showed that the best dose of vitamin E to reach gonad maturity was 375 mg kg-1 with six weeks maintenance. The percentage of gonad maturity was 100%, IGS was 11.50%, IHS was 0.34%, fecundity was 23.484 eggs/individual, the diameters of the eggs were 0.31 mm, and the percentage of mature eggs was 64.44%. These results supported the potential of feed additives such as vitamin E plays a critical role in enhancing gonad maturation in nilem fish thereby providing methods to improve fish reproduction

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Reproduction of the turban shell Turbo torquatus Gmelin 1791 (Mollusca : Gastropoda), in New South Wales, Australia

Marine and Freshwater Research ◽

10.1071/mf00066 ◽

2002 ◽

Vol 53 (1) ◽

pp. 85 ◽

Cited By ~ 8

Author(s):

D. W. Ward ◽

A. R. Davis

Keyword(s):

Reproductive Cycle ◽

Developmental Stages ◽

New South ◽

Gonadosomatic Index ◽

New South Wales ◽

Gonad Development ◽

Gonadal Development ◽

Small Scale ◽

Frequency Distributions ◽

South Wales

The Sydney turban shell Turbo torquatus is the focus of a small-scale commercial fishery in New South Wales. Effective management requires knowledge of the reproductive biology, yet this is lacking for NSW waters. The reproductive cycle was investigated at three localities on the southern New South Wales coast. Samples of T. torquatus were collected monthly at Wollongong, Ulladulla and Eden from February 1996 until August or December 1997. The reproductive cycle was investigated by three methods: monthly determination of a gonadosomatic index, estimation of oocyte size-frequency distributions and classification of female gonads into developmental stages following histological sectioning. Males and females within a population underwent synchronous gonad development and spawning. Spawning events were often protracted over a period of several months with females in various stages of gonadal development. Two spawning events occurred each year, with a spawning event in autumn–winter and another in spring–summer. These events were asynchronous among the three localities, and partial spawning appeared to be a common occurrence. Owing to variation in the timing of spawning between populations separated by a distance as small as 15 km, seasonal closures to protect spawning stocks are unlikely to be effective.

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Pengaruh ablasi mata terhadap kecepatan kematangan gonad kepiting bakau (Sylla serrata) betina

Acta Aquatica Aquatic Sciences Journal ◽

10.29103/aa.v1i1.292 ◽

2014 ◽

Vol 1 (1) ◽

pp. 14

Author(s):

Muhammad Robi ◽

Erlangga Erlangga

Keyword(s):

Survival Rate ◽

Economic Value ◽

Gonadal Development ◽

Scylla Serrata ◽

Mud Crab ◽

Average Value ◽

Gonadal Maturity ◽

Mangrove Crab ◽

Randomized Design ◽

Completely Randomized Design

Kepiting bakau (Scylla serrata) merupakan jenis golongan Crustaceae yang mengandung protein hewani yang cukup tinggi dan memiliki nilai ekonomis yang tinggi. Ablasi mata merupakan salah satu cara untuk menghilangkan hambatan perkembangan telur (gonad) pada kepiting bakau. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) non factorial dengan 4 perlakuan dan 3 ulangan. Adapun perlakuan dalam penelitian ini adalah perlakuan A (ablasi satu pasang mata), perlakuan B (ablasi mata kiri), C (ablasi mata kanan), D (tanpa ablasi). Hasil penelitian menunjukkan bahwa kecepatan kematangan gonad tertinggi pada perlakuan C (ablasi mata kanan) yaitu pertumbuhan gonad mencapai 21,53% dan terendah terjadi D (tanpa ablasi) pertumbuhan gonad hanya 14,8%. Analisa statistik dengan uji F menunjukan bahwa ablasi mata berpengaruh terhadap kecepatan kematangan gonad kepiting bakau. Pertambahan bobot tertinggi terdapat pada perlakuan C (ablasi mata kanan) yaitu sebesar 2,67 gr kemudian disusul oleh D (tanpa ablasi) sebesar 1,89 gr dan terakhir pada B (ablasi mata kiri) dengan nilai rata-rata sebesar 0,77 gr. Tingkat kelangsungan hidup terbaik terdapat pada perlakuan D (tanpa ablasi) yaitu 100%, perlakuan B (ablasi mata kiri) 77,77%, perlakuan C (ablasi mata kanan) 66,66% dan kelansungan hidup terendah terjadi pada perlakuan A (ablasi satu pasang mata) 0%. Rata-rata kualitas air selama penelitian adalah suhu 27 0C dan Ph 7,65.Mangrove crab (Scylla serrata) have been classifying in Crustaceae clas that containing high animal protein and high economic value. Ablation of the eye is an solution to eliminate the egg development (gonads) barriers in the mangrove crab. The research design used a completely randomized design (CRD) non- factorial with 4 treatments and 3 replications. Treatment in this study were the treatment of A (ablation of the pairs of eyes), treatment B (left eye ablation), treatment C (right eye ablation), D (without ablation). The results showed that the highest rate of gonadal maturation was in treatment C (right eye ablation) where gonadal development growth reached 21.53 %, whereas the lowest was in treatment D (without ablation) where gonadal development growth was only 14.8 %. Statistical analysis by F test showed that ablation of the eye was affected the mud crab gonadal maturity. The Weight gain was found highest in the treatment C (right eye ablation) that reached 2.67 g and followed by treatment D (without ablation) 1.89 grams and the last in the treatment B (left eye ablation) with was an average value 0.77 grams. The higest survival rate was found in treatment D (without ablation) 100 %, treatment B (left eye ablation) 77.77 %, treatment C (right eye ablation) 66.66 % and the lowest survival rate was occurred in treatment A (ablation of the pairs of eyes) 0 %. The avarge values of water quality during the study showed that the temperature was reched 27 0C and pH was 7, 65.

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Induced maturation of eel weighed 100–150 gram with PMSG, antidopamine, and 17α-methyltestosterone

Jurnal Akuakultur Indonesia ◽

10.19027/jai.14.135-143 ◽

2015 ◽

Vol 14 (2) ◽

pp. 135 ◽

Cited By ~ 1

Author(s):

Nadia Mega Aryani ◽

Agus Oman Sudrajat ◽

Odang Carman

Keyword(s):

Gonadosomatic Index ◽

Size Variation ◽

Hormonal Treatment ◽

Fish Length ◽

Gonadal Maturation ◽

Pregnant Mare Serum Gonadotropin ◽

Gonadal Histology ◽

Fish Weight ◽

Serum Gonadotropin ◽

Index Value

ABSTRACT Marketed eel Anguilla bicolor bicolor is commonly produced from larvae rearing activity whose broodstocks and larvae are caught from the nature. Supply of eel broodstock is restricted by its life cycle and uncertain size variation of mature male and female. This study was aimed to evaluate the effect of hormonal induction through injection to enhance masculinization and gonadal maturation of eel at the weight of 100–150 g. The experiment used completely random design with the use of combination pregnant mare serum gonadotropin (PMSG), antidopamine (AD), dan 17α-methyltestosterone (MT), which were (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), and P5 (control; without hormonal treatment). The result showed that an increasing of fish length along with fish weight were performed by treatment P4 and P3. The highest gonadosomatic index value was obtained by treatment P3 (1,3030±0,24262). Based on gonadal histology analysis, 2nd phase of spermatogonia development was found in P3 in week-8. The highest testosterone level was obtained by treatment P3, followed by P4, P2, and P1 in week-4. Combination of 10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT could enhance masculinization and gonadal maturation of eel in eight weeks of rearing period. Keywords: gonadal maturation, Anguilla bicolor bicolor, PMSG, AD, MT ABSTRAK Ikan sidat Anguilla bicolor bicolor yang dipasarkan pada umumnya merupakan hasil usaha pembesaran yang benih dan induknya masih diperoleh dari alam. Penyediaan induk ikan sidat terkendala dengan siklus hidup dan variasi perbedaan ukuran induk ikan sidat jantan dan betina matang gonad yang belum pasti. Penelitian ini bertujuan untuk mengkaji peran induksi hormonal yang disuntikkan pada ikan sidat dalam mempercepat proses pematangan gonad ikan sidat ukuran 100–150 g. Metode penelitian yang digunakan adalah metode eksperimen rancangan acak lengkap dengan kombinasi pregnant mare serum gonadotropin (PMSG), antidopamin (AD), dan 17α-metiltestosteron (MT) sebagai berikut P1 (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), dan P5 (kontrol; tanpa perlakuan hormon). Hasil penelitian menunjukkan peningkatan pertambahan panjang seiring dengan pertambahan bobot diperoleh dari perlakuan P4 dan P3. Indeks gonadosomatik tertinggi diperoleh dari perlakuan P3 (1,3030±0,24262). Hasil histologi gonad ditemukan perkembangan spermatogonia fase 2 pada P3 di minggu kedelapan. Konsentrasi testosteron tertinggi didapat dari perlakuan P3 kemudian diikuti P4, P2, dan P1 pada minggu keempat. Kombinasi hormon 10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT dapat mempercepat pematangan gonad dan pertumbuhan pada ikan sidat selama delapan minggu pemeliharaan. Kata kunci: pematangan gonad, Anguilla bicolor bicolor, PMSG, AD, MT

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