scholarly journals Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus

2016 ◽  
Vol 15 (2) ◽  
pp. 99 ◽  
Author(s):  
Wiwik Susanti ◽  
Agustin Indrawati ◽  
Fachriyan H Pasaribu
Keyword(s):  
Inflammatory Cell ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Fatty Degeneration ◽  
Edwardsiella Ictaluri ◽  
Bacterial Disease ◽  
Internal Organs ◽  
Hydropic Degeneration ◽  
Striped Catfish ◽  
Enteric Septicemia

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">One of major problem of striped catfish <em>Pangasionodon hypophthalmus</em> culture is enteric septicemia of catfish (ESC), bacterial disease of <em>Edwardsiella ictaluri</em>, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate <em>E. ictaluri</em>. Thirty individu of five group fishes, 6–10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 10<sup>2</sup> cfu/mL; 10<sup>4</sup> cfu/mL; 10<sup>6</sup> cfu/mL; 10<sup>8</sup> cfu/mL; 10<sup>10</sup> cfu/mL; and PBS as control, were culture in 18 of 60×40×45 cm<sup>3</sup> aquarium for seven days. External organs of fish (skin and abdomen) and internal organs (liver, kidney, and brain) were examined macroscopicly and microscopicly. Internal organ sample were taken on the 5<sup>th</sup> day for histopatologic test while blood sample was on the 1<sup>st</sup>, 3<sup>rd</sup>, and 5<sup>th</sup> day after infection. Mortality rate was count to reach LD50. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano <em>macrophage</em> center (MMC) and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P&lt;0,05) compared to controls. LD50 dose was 2,8×10<sup>4</sup> cfu/mL. The result indicated that <em>E. ictaluri</em> was very pathogenic on striped catfish <em>P. hypophthalmus</em>. </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Edwardsiella ictaluri</em>, enteric septicemia of catfish (ESC), pathogenicity, striped catfish</p><p> </p><p> </p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Salah satu kendala yang dijumpai pada budidaya ikan patin <em>Pangasionodon hypophthalmus</em> yaitu serangan penyakit bakterial. <em>Enteric septicemia of catfish</em> (ESC) adalah penyakit infeksi bakteri <em>Edwardsiella ictaluri</em> yang dapat menyebabkan kematian ikan patin sampai &gt;50%. Penelitian ini dilakukan untuk mengetahui patogenisitas <em>E. ictaluri</em> isolat lokal pada ikan patin. Masing-masing 30 ekor ikan patin ukuran 6–10 g/ekor diinjeksi secara intraperitoneal dengan 0,1 mL larutan bakteri kepadatan 10<sup>2</sup> cfu/mL; 10<sup>4</sup> cfu/mL; 10<sup>6</sup> cfu/mL; 10<sup>8</sup> cfu/mL; 10<sup>10</sup> cfu/mL; dan PBS sebagai kontrol. Ikan dipelihara selama tujuh hari pada akuarium berukuran 60×40×45 cm<sup>3</sup>. Pemeriksaan makroskopis dan mikroskopis dilakukan terhadap organ eksternal (kulit dan abdomen) dan internal (hati, ginjal, dan otak). Pengambilan sampel organ internal untuk uji histopatologi pada hari kelima dan sampel darah untuk uji gambaran darah pada hari pertama, ketiga, dan kelima pascainfeksi. Jumlah kematian ikan dihitung untuk mendapat nilai LD50. Pengamatan gejala klinis dan patologi anatomi ditemukan ikan berenang vertikal, adanya bercak merah pada kulit, pembengkakan abdomen, asites, hati pucat, dan ginjal berwarna merah kehitaman. Hasil histopatologi terlihat terjadinya degenerasi hidropik, degenerasi lemak, <em>melano macrophage center</em> (MMC), nekrosa, hemoragi, dan infiltrasi sel radang pada hati, ginjal, dan otak. Penurunan nilai hematokrit dan hemoglobin pada perlakuan secara statistik berbeda nyata (P&lt;0,05) dengan kontrol. Dosis LD50 didapat 2,8×10<sup>4</sup> cfu/mL. Hasil penelitian ini mengindikasikan bahwa <em>E. ictaluri</em> pada ikan patin bersifat sangat patogen.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: <em>Edwardsiella ictaluri</em>, <em>enteric septicemia of catfish </em>(ESC), patogenisitas, patin</p>

scholarly journals Importance and Antimicrobial Resistance of Mycoplasma bovis in Clinical Respiratory Disease in Feedlot Calves

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1470
Author(s):  
Ana García-Galán ◽  
Juan Seva ◽  
Ángel Gómez-Martín ◽  
Joaquín Ortega ◽  
Francisco Rodríguez ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Clinical Signs ◽  
Antimicrobial Treatment ◽  
Mycoplasma Bovis ◽  
Bacterial Disease ◽  
Feedlot Calves ◽  
Fixed Panel

Bovine respiratory disease (BRD) is an important viral and/or bacterial disease that mainly affects feedlot calves. The involvement of Mycoplasma bovis in BRD can lead to chronic pneumonia poorly responsive to antimicrobial treatment. Caseonecrotic bronchopneumonia is a pulmonary lesion typically associated with M. bovis. In Spain, M. bovis is widely distributed in the feedlots and circulating isolates are resistant to most antimicrobials in vitro. However, the role of this species in clinical respiratory disease of feedlot calves remains unknown. Furthermore, available data are relative to a fixed panel of antimicrobials commonly used to treat BRD, but not to the specific set of antimicrobials that have been used for treating each animal. This study examined 23 feedlot calves raised in southeast Spain (2016–2019) with clinical signs of respiratory disease unresponsive to treatment. The presence of M. bovis was investigated through bacteriology (culture and subsequent PCR), histopathology and immunohistochemistry. The pathogen was found in 86.9% (20/23) of the calves, mainly in the lungs (78.26%; 18/23). Immunohistochemistry revealed M. bovis antigens in 73.9% (17/23) of the calves in which caseonecrotic bronchopneumonia was the most frequent lesion (16/17). Minimum inhibitory concentration assays confirmed the resistance of a selection of 12 isolates to most of the antimicrobials specifically used for treating the animals in vivo. These results stress the importance of M. bovis in the BRD affecting feedlot calves in Spain.

scholarly journals Ehlers–Danlos syndrome: how to diagnose and when to perform genetic tests

2014 ◽  
Vol 100 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Glenda Sobey
Keyword(s):  
Genetic Basis ◽  
Correct Diagnosis ◽  
Clinical Signs ◽  
Accurate Diagnosis ◽  
Connective Tissue Disorders ◽  
Internal Organs ◽  
Ehlers Danlos Syndrome ◽  
Starting Point ◽  
Life Threatening ◽  
Danlos Syndrome

The term Ehlers–Danlos syndrome (EDS) encompasses a group of inherited connective tissue disorders. The manifestations of EDS can be seen in skin, joints, blood vessels and internal organs and vary from mild to severe and life threatening. Each subtype is a separate and different condition. The genetic basis of many subtypes has now been elucidated, confirming heterogeneity. An awareness of the different conditions within this group is the starting point towards accurate diagnosis. Accurate elicitation of history and clinical signs is vital in selecting the correct confirmatory investigation. Skin biopsy with electron microscopy can be helpful in the decision process of whether and when to perform genetic testing. Correct diagnosis within the EDSs allows targeted management, family screening and prenatal diagnosis.

scholarly journals Acute fasciolosis in cattle in southern Brazil

2013 ◽  
Vol 33 (6) ◽  
pp. 705-709 ◽  
Author(s):  
Maria de Lourdes Adrien ◽  
Ana Lucia Schild ◽  
Clairton Marcolongo-Pereira ◽  
Letícia Fiss ◽  
Jerônimo L. Ruas ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Early Spring ◽  
Eosinophil Infiltration ◽  
Coagulation Necrosis ◽  
Economic Losses ◽  
Southern Brazil ◽  
Bile Duct Proliferation ◽  
Mild Anemia

This study describes the epidemiological and pathological aspects of an outbreak of acute fasciolosis in cattle in southern Brazil. Fifteen out of 70 three-year-old pregnant cows lost weight in the 30-40 days prior to calving. Clinical signs included diarrhea, weakness, mild anemia and jaundice. Dark yellow fluid in the abdominal cavity was observed at necropsy. Fibrin and clotted blood were adhered to the pericardium and lung, primarily in the diaphragmatic lobes. The liver was enlarged, and the capsular surface was irregular with clear areas and petechiae. At the cut surface, the liver was irregular, firm and edematous, and several hemorrhagic channels could be observed. Areas of fibrosis through the parenchyma and whitish thrombi occluding the great vessels were also observed. The livers of 10 cows that not died were condemned at slaughter for lesions of fasciolosis similar to those observed at necropsy. Microscopically, the liver showed areas of coagulation necrosis, extensive hemorrhages in the streaks or foci and disruption of the parenchyma with neutrophil and eosinophil infiltration. Fibrosis and bile duct proliferation were also observed. Immature Fasciola hepatica flukes were observed in the parenchyma surrounded by degenerated hepatocytes, neutrophils, eosinophils, and hemorrhages. The outbreak occurred on a farm located in an area endemic for fasciolosis, although the acute form of the disease is not common in cattle in this region. It is likely that the cows were infected by F. hepatica metacercariae released in the late fall or early spring in the rice stubble where the herd was grazing prior to calving. Although mortality due to fasciolosis in cattle is infrequent, outbreaks can occur and treatments that are effective in both the immature and adult forms of the parasite should be administered to prevent economic losses.

scholarly journals Application of a temporary intestinal storage device in a small intestine gunshot wound dog model

2021 ◽  
Author(s):  
Weihang Wu ◽  
Mingwei Wang ◽  
Weikang Zhou ◽  
Yuewen Zhu ◽  
Tianyu Lin ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gunshot Wound ◽  
Inflammatory Cell ◽  
Treatment Time ◽  
Abdominal Cavity ◽  
First Aid ◽  
Storage Device ◽  
Wound Model ◽  
Transmission Electron ◽  
Normal Intestine

Abstract Background: We aimed to verify the feasibility of a novel temporary intestinal storage device (TISD) using a simple intestinal gunshot wound model. Methods: Ten female beagle dogs were fasted for 12 hours and anesthetized. An incision protector was inserted into a 10-cm abdominal incision. The small intestine was exposed to the body by natural drooping. An automatic rifle was used to shoot the intestine from a distance of 25 meters to introduce a simple intestinal gunshot wound. The three phases of first aid for war injuries were followed: Care Under Fire, Tactical Field Care, and Tactical Evacuation Care. For Tactical Field Care, a novel TISD was used to reconstruct the ruptured intestine, and necrotic intestinal tissue was stored. The abdominal cavity was temporarily closed, and the abdomen was opened for exploration 4 hours after surgery. Treatment time was observed during Care Under Fire, transfer time was observed from Tactical Field Care to Tactical Evacuation Care, rescue was observed during Tactical Evacuation Care, and the treatment time of each intestinal segment was measured. After 4 hours, intestinal vitality was observed, and the heart, liver, spleen, lung, kidney, stomach, normal intestine, and necrotic intestine were examined before and 4 hours after surgery by light microscopy. The broken ends of the intestine were connected to the intestinal reconstruction device before and 4 hours after surgery and were examined by transmission electron microscopy. Results: The processing time of Care Under Fire was 41.55 ± 10.46 seconds, which is shorter than the maximum time limit of the battlefield first aid principle. Transit time from Care Under Fire to Tactical Field Care transit was 60.78 ± 15.95 seconds, which is shorter than the battlefield first aid principle. The treatment time of Tactical Field Care was 29.75 ± 5.13 minutes, and the reconstruction time of each intestinal segment was 4.44 ± 0.31 minutes. One dog died of anesthetic overdose, two died of splenic bleeding, and the rest completed all phases. The abdominal cavity was explored 4 hours after surgery, and the TISD was positioned. Intestinal tract reconstruction was normal, and no obvious necrosis was observed. Necrotic intestine had the same vitality as before storage. With light microscopy, the heart, liver, spleen, lung, kidney, and stomach showed no obvious necrosis, inflammatory cell infiltration, or necrosis of normal intestine before and after surgery. Before and 4 hours after surgery, intestinal necrosis involved local necrosis of villi and tissues, and marked inflammatory cell infiltration. Transmission electron microscopy showed that the villi of the intestinal stump connected to the TISD before surgery were intact, and no obvious necrosis was observed. The villi of the intestinal stump were moderately damaged after surgery, and focal necrosis was observed. Conclusions: The novel TISD can be used in the emergency treatment of simple small intestine gunshot wounds in beagle dogs and can prevent further deterioration after intestinal injury. Background: We aimed to verify the feasibility of a novel temporary intestinal storage device (TISD) using a simple intestinal gunshot wound model.

scholarly journals MIGRATION OF THE INTRAUTERINE DEVICE TO THE ABDOMINAL CAVITY

2017 ◽  
Vol 25 (2) ◽  
pp. 247-262
Author(s):  
D. A. Rahmonov ◽  
F. Sh. Rashidov ◽  
E. L. Kalmykov ◽  
M. M. Marizoeva ◽  
O. B. Bobdjonova ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Intrauterine Device ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Uterine Cavity ◽  
Pain Syndrome ◽  
Pregnant Patient ◽  
Pelvic Cavity ◽  
And Migration ◽  
Laparoscopic Removal

The aim: demonstration of our experience of surgical treatment of patients with migrated intrauterine device (IUD) into the abdominal cavity. The results of surgical treatment of migrated IUDs in the pelvic cavity are summarized in 17 women. The average age of the patients was 33,23,4 years. The timing of implantation of the IUDs varied from 10 days to 24 months. In all cases, the intra operational finding was T-shaped a copper device. The reason behind the women's consultation was an increase in pain syndrome in the lesser pelvis (n=15), dysuric phenomenon (n=1) and the onset of pregnancy (n=1). Perforation of the uterus and migration of the spiral occurred from 10 days to 2 years after its implantation. All patients were operated laparoscopicaly. The average duration of operations was 45,510,5 minutes. In the postoperative period there were no complications from the pelvic organs and postoperative wounds. The period of hospitalization of patients was 3,50,7 days. In all cases there was a regression of clinical signs and recovery. In one pregnant patient (gestation period 5-6 weeks) the pregnancy proceeded without particular pathological abnormalities and resulted in the birth of a full-term child. Laparoscopic removal of the IUD migrating from the uterine cavity to the abdominal cavity is the method of choice in the treatment of this group of patients, avoiding development of intra- and postoperative complications and a shorter length of stay in the hospital. The effectiveness of the procedure reaches 100%. The most common cause of complication of the IUD is the perforation of the uterus during its implantation.

Intestinal lymphoma in a Boxer breed dog – case report

2019 ◽  
Vol XXIV (142) ◽  
pp. 72-82
Author(s):  
Sayonara da Luz Ferro ◽  
Fernanda Jönck ◽  
Marta Cristina T. Heckler ◽  
Ewerton Cardoso ◽  
Mateus Rychescki ◽  
...  
Keyword(s):  
Weight Loss ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Exploratory Laparotomy ◽  
Needle Aspiration ◽  
Free Fluid ◽  
Intestinal Lymphoma ◽  
Mesenteric Lymph Nodes ◽  
Abdominal Ultrasonography ◽  
Tissue Biopsy

Intestinal lymphoma is the second most common form of lymphoma in dogs. It is characterized by the presence of neoplastic lymphocytes in the gastrointestinal tract or mesenteric lymph nodes. The chief clinical signs are vomiting, diarrhea, anorexia and weight loss. The diagnosis is made through fine needle aspiration and cytology, or by tissue biopsy and histopathology, and chemotherapy is the treatment of choice. We report a case of intestinal lymphoma in a five years old Boxer, presented with history of hematochezia and lethargy of 7 days duration, and 20 days of anorexia and persistent weight loss. Abdominal ultrasonography revealed free fluid in the abdominal cavity, and an intestinal mass. Exploratory laparotomy was performed and a tissue biopsy was performed. Histopathology confirmed the diagnosis of intestinal lymphoma, but the patient died during the surgical procedure.

Gastrointestinal Stromal Tumors and Leiomyomas in the Dog: A Histopathologic, Immunohistochemical, and Molecular Genetic Study of 50 Cases

2003 ◽  
Vol 40 (1) ◽  
pp. 42-54 ◽  
Author(s):  
D. Frost ◽  
J. Lasota ◽  
M. Miettinen
Keyword(s):  
Smooth Muscle ◽  
Gastrointestinal Stromal Tumors ◽  
Smooth Muscle Actin ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Muscle Actin ◽  
Molecular Genetic Study ◽  
Stromal Tumors ◽  
Pathologic Features ◽  
S 100

Fifty canine gastrointestinal (GI) mesenchymal tumors were examined to determine the occurrence of leiomyomas (LM) and GI stromal tumors and to compare their clinicopathologic features. Twenty-one tumors (42%) were histologically reclassified as gastrointestinal stromal tumors (GISTs) and 29 tumors (58%) as LMs on the basis of their histologic similarity with homologous human tumors. The GISTs occurred equally in males and females, with a mean age of 11 years (range 5–14 years). Five GISTs (24%) were associated with clinical signs and six (29%) had metastasis in liver or abdominal cavity. The GISTs occurred in large intestine (10, 48%), small bowel (six, 29%), stomach (four, 19%), and mesentery of small intestine (one, 5%). Histologically, they were highly cellular spindle, or less commonly epithelioid tumors with mitotic rates ranging from 0 to 19 per 10 HPF. Eleven tumors (52%) were positive for CD117 (KIT); seven (33%) were positive for smooth muscle actin but none for desmin and S-100 protein. Sequences of KIT exon 11, often mutated in human GISTs, were evaluated from four GISTs. Deletion of Try556-Lys557 coexisting with duplication of Gln555 in one case of GIST and T to C transition resulting in substitution of Pro for Leu575 in another were identified. The LMs occurred predominantly in males (82%) with a mean age of 11 years (range 8–17 years). Nine tumors (31%) had associated clinical signs. They occurred in the stomach (22, 76%), esophagus (four, 14%), and intestines (three, 10%); all were paucicellular, had no mitoses, and were composed of mature smooth muscle cells. Twenty-eight (97%) were positive for smooth muscle actin and 18(62%) for desmin but none for CD117 and S-100. Both GISTs and true LMs occur in the GI tract of dogs. Both tumors have distinctive pathologic features.

scholarly journals Susceptibility and pathological consequences of catla, Catla catla (Hamilton) experimentally infected with Edwardsiella tarda

2016 ◽  
Vol 24 (4) ◽  
pp. 209-217 ◽  
Author(s):  
Thongam Bidya Devi ◽  
T. Jawahar Abraham ◽  
Dibyendu Kamilya
Keyword(s):  
Lethal Dose ◽  
Clinical Signs ◽  
Infected Fish ◽  
Edwardsiella Tarda ◽  
Catla Catla ◽  
Cell Hyperplasia ◽  
Hydropic Degeneration ◽  
Leucocyte Infiltration ◽  
Gill Lamellae ◽  
Kidney Liver

AbstractThe present study tested the susceptibility and pathological changes of catla, Catla catla (Hamilton) infected with Edwardsiella tarda (ET-PG-29). The bacterium was isolated from the kidney of a diseased pangas catfish. To determine the median lethal dose (LD50), C. catla were challenged with this bacterium (108-103CFU ml-1), and the LD50was calculated as 105.5CFU ml-1. Another set of healthy C. catla were injected intraperitoneally with the LD50dose to induce edwardsiellosis. The clinical signs of the infected C. catla were observed and recorded. Tissues such as kidney, liver, intestine, heart, and gill from the infected fish with clinical signs of edwardsiellosis were used for histopathology. The clinical and gross signs were first visible at 1 d post-injection, and the infected fish showed typical signs of hemorrhagic septicemia. The most striking histopathological features were found in the kidney which showed multi-focal necrosis with the formation of granuloma indicating an inflammatory response against the pathogen. The intestine displayed goblet cell hyperplasia, the liver showed hydropic degeneration with hyperemic central veins, and there was inflammation of gill lamellae and cardiac myositis associated with leucocyte infiltration. Collectively, the results confirmed the susceptibility of C. catla to E. tarda infection and that this bacterium is a threat to C. catla in aquaculture practices.

scholarly journals Total prostatectomy combined with urethral anastomosis in a dog: a case report

2019 ◽  
Vol 64 (No. 6) ◽  
pp. 280-286
Author(s):  
P Przadka ◽  
B Liszka ◽  
A Piatek ◽  
P Skrzypczak ◽  
S Dzimira ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Abdominal Cavity ◽  
Prostate Gland ◽  
Companion Animals ◽  
Clinical Signs ◽  
Skeletal System ◽  
Malignant Tumours ◽  
Neoplastic Process ◽  
Laparotomy Wound ◽  
Total Prostatectomy

Prostate cancer is the most common prostate disease diagnosed in castrated dogs. However, prostate cancer is considered to be quite rare in dog population. The presence of prostate tumours can lead to urethral obstruction. Other clinical signs include asymmetric, painful, hard growths on rectal examination, neurological deficit or lameness of the pelvic limbs and general symptoms such as anorexia, weight loss or difficult urination. The neoplastic process may spread to the neighbouring organs and give metastases to the lymph nodes, lungs and skeletal system. Patient with histopathologically confirmed prostate cancer should be examined for metastases. The prognosis is usually unfavourable because of late diagnosis and the presence of metastases. Therapeutic options include surgery, chemotherapy and radiotherapy. Complete prostatectomy carries with it many complications and often requires osteotomy of pelvic fusions. For that reason, total prostatectomy is not commonly performed in companion animals; however, it should be considered in cases of early detection with the absence of metastases. This report presents the procedure of a complete prostatectomy in a dog with a pre-pubic anastomosis of the urethra, without osteotomy of the pelvic septum. The anastomosis of the urinary tract was placed outside the abdominal cavity, caudally from the laparotomy wound. The presented technique seems to be appropriate for the treatment of malignant tumours of the prostate gland in dogs.

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