scholarly journals Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)

2017 ◽  
Vol 16 (1) ◽  
pp. 92 ◽  
Author(s):  
, Sukenda ◽  
Kiki Amalia Pratiwi ◽  
, Rahman ◽  
Dendi Hidayatullah
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Passive Immunization ◽  
Relative Survival ◽  
Control Fish ◽  
Cell Vaccine ◽  
Relative Survival Rate ◽  
Whole Cell Vaccine ◽  
Antibody Titers ◽  
And Control

<p class="Pa3"><strong>ABSTRACT </strong></p><p> </p><p class="Pa5">Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of <em>Aeromonas hydrophila </em>vaccine on the catfish broodstock to maternal transfer of immunity, and offspring resistance. The average body weight of broodstock used in this study were 650±50 g were kept in pool tarps sized 2×1×0.5 m3. This study used a randomized complete design with two treatments and three replications. Female broodstock were vaccinated using intraperitonial injections at a dose 0.4 mL/kg and control fish were injected with phospate buffered saline (PBS). The observed parameters include hematology of broodstock, mortality, the relative survival rate, and antibody titers. Antibody titer measurements on broodstock, eggs, and offspring. Vaccination on broodstock catfish delivers a significant antibody level (P&lt;0.05) on offspring compared to control catfish with relative survival rate of offspring at 5, 10, and 15 days after hatching were 67.76%, 82.66%, and 71.66% respectively.</p><p> </p><p class="Pa5">Keywords: catfish, <em>Aeromonas hydrophila</em>, vaccination, antibody transfer</p><p> </p><p> </p><p class="Pa3"><strong>ABSTRAK </strong></p><p> </p><p class="Pa5">Transfer kekebalan dari induk kepada benih melalui imunisasi pasif merupakan salah satu cara untuk memberikan proteksi pada benih. Tujuan penelitian ini untuk menguji efikasi vaksin sel utuh <em>Aeromonas hydrophila </em>pada induk ikan nila dalam mentransfer kekebalan spesifik ke benih dan menguji ketahanan benih hasil pemijahan induk yang divaksin. Induk lele yang digunakan pada penelitian ini memiliki bobot rata-rata 650±50 g dipelihara di kolam terpal berukuran 2×1×0,5 m3. Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Induk betina lele divaksinasi secara intraperitonial dengan dosis 0,4 mL/kg ikan dan induk lele kontrol disuntik dengan phospate buffer saline (PBS). Parameter yang diamati meliputi hematologi induk, mortalitas, tingkat kelangsungan hidup relatif benih, dan titer antibodi. Vaksinasi induk lele memberikan hasil level antibodi yang signifikan (P&lt;0,05) pada induk, telur, dan benih lele dibandingkan perlakuan kontrol dengan tingkat kelangsungan hidup relatif benih umur 5, 10, dan 15 hari pacatetas masing-masing sebesar 67,76; 82,66%; dan 71,66%.</p><p> </p><p>Kata kunci: ikan lele, <em>Aeromonas hydrophila</em>, vaksinasi, transfer antibodi</p>

scholarly journals CBTRUS Statistical Report: Primary Brain and Other Central Nervous System Tumors Diagnosed in the United States in 2012–2016

2019 ◽  
Vol 21 (Supplement_5) ◽  
pp. v1-v100 ◽  
Author(s):  
Quinn T Ostrom ◽  
Gino Cioffi ◽  
Haley Gittleman ◽  
Nirav Patil ◽  
Kristin Waite ◽  
...  
Keyword(s):  
United States ◽  
Central Nervous System ◽  
Nervous System ◽  
Survival Rate ◽  
Incidence Rate ◽  
Relative Survival ◽  
The United States ◽  
Population Based ◽  
Cns Tumors ◽  
Relative Survival Rate

AbstractThe Central Brain Tumor Registry of the United States (CBTRUS), in collaboration with the Centers for Disease Control and Prevention and National Cancer Institute, is the largest population-based registry focused exclusively on primary brain and other central nervous system (CNS) tumors in the United States (US) and represents the entire US population. This report contains the most up-to-date population-based data on primary brain tumors available and supersedes all previous reports in terms of completeness and accuracy. All rates are age-adjusted using the 2000 US standard population and presented per 100,000 population. The average annual age-adjusted incidence rate (AAAIR) of all malignant and non-malignant brain and other CNS tumors was 23.41 (Malignant AAAIR = 7.08, non-Malignant AAAIR = 16.33). This rate was higher in females compared to males (25.84 versus 20.82), Whites compared to Blacks (23.50 versus 23.34), and non-Hispanics compared to Hispanics (23.84 versus 21.28). The most commonly occurring malignant brain and other CNS tumor was glioblastoma (14.6% of all tumors), and the most common non-malignant tumor was meningioma (37.6% of all tumors). Glioblastoma was more common in males, and meningioma was more common in females. In children and adolescents (age 0–19 years), the incidence rate of all primary brain and other CNS tumors was 6.06. An estimated 86,010 new cases of malignant and non-malignant brain and other CNS tumors are expected to be diagnosed in the US in 2019 (25,510 malignant and 60,490 non-malignant). There were 79,718 deaths attributed to malignant brain and other CNS tumors between 2012 and 2016. This represents an average annual mortality rate of 4.42. The five-year relative survival rate following diagnosis of a malignant brain and other CNS tumor was 35.8%, and the five-year relative survival rate following diagnosis of a non-malignant brain and other CNS tumors was 91.5%.

EFIKASI RUTE VAKSIN Aeromonas hydrophila ASB-01 PADA IKAN GABUS (Ophiocephalus striatus)

2016 ◽  
Vol 3 (6) ◽  
pp. 131
Author(s):  
Olga Olga ◽  
Fatmawaty Fatmawaty
Keyword(s):  
Antibody Titer ◽  
Aeromonas Hydrophila ◽  
Survival Rates ◽  
Booster Vaccination ◽  
Time Of Death ◽  
Blood Draw ◽  
Percent Survival ◽  
Antibody Titers ◽  
And Control ◽  
Mean Time

Penelitian ini bertujuan untuk mengetahui rute pemberian vaksin A.hydrophila ASB-01 yang efektif untuk mengendalikan MAS pada ikan gabus.  Efektivitas rute vaksinasi dievaluasi melalui titer antibodi, sintasan, RPS (relative percent survival) dan RWK (Rerata waktu kematian).  Penelitian ini terdiri dari 5 perlakuan (vaksinasi secara rendaman (R), oral (O), injeksi intramuscular (IM), injeksi intraperitoneal (IP) dan Kontrol (PBS pH 7,0) dengan  3 ulangan. Dosis vaksinasi sebanyak 107 sel/ml. Vaksinasi booster dilakukan setelah 14 hari kemudian, dosisnya sama dengan vaksinasi awal.  Selanjutnya, 14 hari berikutnya ikan ditantang dengan A.hydrophila ASB-01. Untuk memperoleh data titer antibodi dilakukan pengambilan darah pada saat sebelum divaksinasi, sesaat sebelum vaksinasi booster dan 14 hari setelah vaksinasi booster. Ikan tantang diamati selama 14 hari untuk memperoleh data sintasan, RPS dan RWK. Hasil menunjukkan bahwa semua rute pemberian vaksin dapat meningkatkan titer antibodi, akan tetapi titer antibodi tertinggi diperoleh dari ikan yang divaksinasi secara injeksi.  Sintasan gabus yang divaksinasi secara IM (84,47%), IP (82,20%), R (42,27%), O (42,20%) dan kontrol (13,13 %). RPS gabus yang divaksinasi melalui rute IM (82,08%), IP (79,46%), R (33,38%), O (33,31%), sedangkan RWK gabus melalui rute IP (3,63 hari), IM (79,46 3,57 hari), R (2,46 hari), O (1,85 hari) dan kontrol (1,03 hari). Rute vaksinasi yang efektif adalah melalui injeksi.This study aims to determine the vaccine A.hydrophila ASB-01 is effective for the control of MAS on snakehead fish. Effectiveness of vaccination was evaluated through the antibody titer, survival, RPS (relative percent survival) and RWK (mean time of death). The study consisted of 5 treatments (immersion vaccination (R), oral (O), intramuscular injection (IM), intraperitoneal injection (IP) and control (PBS pH 7.0) with 3 replications. Vaccination doses were 107 cells / ml. Booster vaccination after 14 days later. Dose is equal to the initial vaccination. Furthermore, the next day 14 fish were challenged with A.hydrophila ASB-01. To obtain data on antibody titer blood draw done at the time before being vaccinated, shortly before the booster vaccination and 14 days after the booster vaccination. Challenged fish were observed for 14 days to obtain data on survival, RPS and RWK.  The result showed that all these vaccines may increase the antibody titer, but the highest antibody titers obtained from fish vaccinated injection. Survival rates were vaccinated IM (84.47%), IP (82.20%), R (42.27%), O (42.20%) and controls (13.13%). RPS to IM (82.08%), IP (79.46%), R (33.38%), O (33.31%), while RWK through the IP (3.63 days), IM (79.46 3.57 days), R (2.46 days), O (1.85 days) and controls (1.03 days). Effective vaccination route is through injection.

scholarly journals Vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in its fry against Aeromonas hydrophila

2017 ◽  
Vol 16 (2) ◽  
pp. 268 ◽  
Author(s):  
Sukenda Sukenda ◽  
Odang Carman ◽  
Rahman Rahman ◽  
Dendi Hidayatullah ◽  
Nurfitriani Siti Yumaidawati
Keyword(s):  
Disease Resistance ◽  
Immunosorbent Assay ◽  
Antibody Level ◽  
Aeromonas Hydrophila ◽  
Nile Tilapia ◽  
Cell Vaccine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Whole Cell ◽  
Whole Cell Vaccine ◽  
Phosphate Buffered Saline

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">The aim of this study was to analyze the effectivity of vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in fry tilapia against <em>Aeromonas hydrophila</em>. Tilapia Nirwana strain that used for this had average body weight of 185±13.23 g and were maintained in ponds sizing of (2.5×2.5×1 m<sup>3</sup>). Vaccinations that has been done through intraperitoneal injection using dose of 0.1 mL/fish, meanwhile the fish for control was injected by phosphate buffered saline (PBS). This study used complete randomized design with two treatments and three replications. Antibody level was measured by using indirect enzyme-linked immunosorbent assay (ELISA) method in the broodstock, egg, and fry.  Challenge test in fry tilapia performed at the age of 5, 10, and 15 days. The results showed that vaccination in tilapia broodstock delivered a significant antibody level in broodstock, eggs, and fry (P&lt;0.05) compared to the control. Relative percent survival of offspring at 5, 10, and 15 days were 78.26%, 70.59%, and 65.52%, respectively.  As a conclusion, vaccination in tilapia broodstock was effective to improve specific and non-specific immunity, and protect fry tilapia from <em>A. hydrophila</em> infection through maternal immunity.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: vaccination, antibody, maternal immunity, tilapia, <em>Aeromonas hydrophila</em></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk menganalisis efikasi vaksinasi pada induk nila dengan vaksin sel utuh dan ketahanan benih yang dihasilkan terhadap <em>Aeromonas hydrophila</em>. Ikan nila stain Nirwana yang digunakan dalam penelitian memiliki bobot rata-rata 185±13,23 g dan ikan dipelihara dalam kolam (2,5×2,5×1 m<sup>3</sup>). vaksinasi dilakukan melalui penyuntikan intraperitoneal dengan dosis 0,1 mL/ikan, sementara itu ikan kontrol disuntik dengan <em>phosphate buffered saline</em> (PBS). Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Tingkat antibodi diukur dengan menggunakan metode<em> indirect enzyme-linked immunosorbent assay</em> (ELISA) pada induk, telur dan benih. Uji tantang pada benih dilakukan pada umur 5, 10, dan 15 hari. Hasil penelitian menunjukan bahwa vaksinasi pada induk nila secara signifikan dapat meningkatkan level antibodi pada induk, telur, dan benih (P&lt;0,05) dibandingkan dengan kontrol. Kelangsungan hidup relatif pada benih berumur 5, 10, dan 15 hari masing-masing adalah 78,26%; 70,59%; dan 65,52%. Sebagai kesimpulan vaksinasi pada induk nila efektif dalam memperbaiki imunitas spesifik dan non spesifik serta melindungi benih dari infeksi <em>A. hydrophila</em> melalui imunitas maternal.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: vaksinasi, antibodi, imunitas maternal, ikan nila, <em>Aeromonas hydrophila</em></p>

scholarly journals Upaya Peningkatkan Pengetahuan Sadari Keluarga Penderita Kanker Payudara dengan Pendekatan Health Beliefe Model

2018 ◽  
Vol 14 (1) ◽  
pp. 35
Author(s):  
Yuniastini Yuniastini ◽  
Ratna Dewi ◽  
Arif Yulinda
Keyword(s):  
Survival Rate ◽  
Self Efficacy ◽  
Relative Survival ◽  
Perceived Barriers ◽  
Motivating Factors ◽  
Perceived Susceptibility ◽  
Relative Survival Rate ◽  
Perceived Seriousness ◽  
Cues To Action

<p>Kanker payudara banyak terjadi pada wanita. Di Lampung, pada tahun 2014,  jumlah penderita kanker payudara adalah yang tertinggi dan sebagian besar datang pada stadium lanjut.  Stadium kanker payudara saat terdiagnosis berhubungan dengan <em>survival rate</em> yang ditimbulkan oleh kanker tersebut. Semakin dini stadium kanker terdiagnosis maka semakin banyak penderita dengan daya tahan hidup selama lima tahun <em>(five-year relative survival rate)</em>. Tujuan penelitian ini meningkatkan pengetahuan tentang kanker payudara dan sadari keluarga penderita  kanker payudara dengan pendekatan <em>Health Beliefe Model </em>(HBM).Penelitian dirancang dengan pendekatan <em>One Group </em><em>Pretest-Posttest</em>.Sampel penelitian adalah sebagian dari keluarga pasien kanker payudara dengan  kriteria:  merupakan keluarga pasien kanker payudara dari satu garis keturunan, berjenis kelamin perempuan, berusia ≥ 20 tahun, dalam kondisisehat dan bersedia berpartisipasi dalam penelitian berjumlah 30 orang. Media yang digunakan video, dirancang dengan pendekatan HBM, yakni dengan memasukkan konstruksi dari HBM yakni <em>perceived seriousness, perceived susceptibility</em>, <em>perceive benefits,perceived barriers, cues to action, motivating factors</em>, <em>self-efficacy</em>. Pengetahuan yang ditayangkan dalam media meliputi pengertian,  tanda dan gejala kanker payudara, faktor resiko kanker payudara, manfaat sadari dan teknik sadari.Analisis yang digunakan  <em>Wilcoxon Signed Ranks Test</em><em>.</em>Hasil penelitian diperoleh adanya  perbedaan antara pengetahuan keluarga penderita kanker payudara sebelum dan sesudah diberikan perlakuan dengan pendekatan HBM (<em>p</em>=0,000 ; positive ranks 22). <em>Kesimpulan, </em>pendekatan HBM secara signifikan dapat meningkatkan pengetahuan keluarga penderita kanker payudara dan disarankan untuk diterapkan.</p>

Sign in / Sign up

Export Citation Format

Share Document