EFIKASI RUTE VAKSIN Aeromonas hydrophila ASB-01 PADA IKAN GABUS (Ophiocephalus striatus)

2016 ◽  
Vol 3 (6) ◽  
pp. 131
Author(s):  
Olga Olga ◽  
Fatmawaty Fatmawaty
Keyword(s):  
Antibody Titer ◽  
Aeromonas Hydrophila ◽  
Survival Rates ◽  
Booster Vaccination ◽  
Time Of Death ◽  
Blood Draw ◽  
Percent Survival ◽  
Antibody Titers ◽  
And Control ◽  
Mean Time

Penelitian ini bertujuan untuk mengetahui rute pemberian vaksin A.hydrophila ASB-01 yang efektif untuk mengendalikan MAS pada ikan gabus.  Efektivitas rute vaksinasi dievaluasi melalui titer antibodi, sintasan, RPS (relative percent survival) dan RWK (Rerata waktu kematian).  Penelitian ini terdiri dari 5 perlakuan (vaksinasi secara rendaman (R), oral (O), injeksi intramuscular (IM), injeksi intraperitoneal (IP) dan Kontrol (PBS pH 7,0) dengan  3 ulangan. Dosis vaksinasi sebanyak 107 sel/ml. Vaksinasi booster dilakukan setelah 14 hari kemudian, dosisnya sama dengan vaksinasi awal.  Selanjutnya, 14 hari berikutnya ikan ditantang dengan A.hydrophila ASB-01. Untuk memperoleh data titer antibodi dilakukan pengambilan darah pada saat sebelum divaksinasi, sesaat sebelum vaksinasi booster dan 14 hari setelah vaksinasi booster. Ikan tantang diamati selama 14 hari untuk memperoleh data sintasan, RPS dan RWK. Hasil menunjukkan bahwa semua rute pemberian vaksin dapat meningkatkan titer antibodi, akan tetapi titer antibodi tertinggi diperoleh dari ikan yang divaksinasi secara injeksi.  Sintasan gabus yang divaksinasi secara IM (84,47%), IP (82,20%), R (42,27%), O (42,20%) dan kontrol (13,13 %). RPS gabus yang divaksinasi melalui rute IM (82,08%), IP (79,46%), R (33,38%), O (33,31%), sedangkan RWK gabus melalui rute IP (3,63 hari), IM (79,46 3,57 hari), R (2,46 hari), O (1,85 hari) dan kontrol (1,03 hari). Rute vaksinasi yang efektif adalah melalui injeksi.This study aims to determine the vaccine A.hydrophila ASB-01 is effective for the control of MAS on snakehead fish. Effectiveness of vaccination was evaluated through the antibody titer, survival, RPS (relative percent survival) and RWK (mean time of death). The study consisted of 5 treatments (immersion vaccination (R), oral (O), intramuscular injection (IM), intraperitoneal injection (IP) and control (PBS pH 7.0) with 3 replications. Vaccination doses were 107 cells / ml. Booster vaccination after 14 days later. Dose is equal to the initial vaccination. Furthermore, the next day 14 fish were challenged with A.hydrophila ASB-01. To obtain data on antibody titer blood draw done at the time before being vaccinated, shortly before the booster vaccination and 14 days after the booster vaccination. Challenged fish were observed for 14 days to obtain data on survival, RPS and RWK.  The result showed that all these vaccines may increase the antibody titer, but the highest antibody titers obtained from fish vaccinated injection. Survival rates were vaccinated IM (84.47%), IP (82.20%), R (42.27%), O (42.20%) and controls (13.13%). RPS to IM (82.08%), IP (79.46%), R (33.38%), O (33.31%), while RWK through the IP (3.63 days), IM (79.46 3.57 days), R (2.46 days), O (1.85 days) and controls (1.03 days). Effective vaccination route is through injection.

scholarly journals Pengaruh Cara Booster terhadap Efikasi Vaksinasi Oral dengan Debris Sel Aeromonas hydrophila pada Lele Dumbo (Clarias sp.)

2006 ◽  
Vol 8 (1) ◽  
pp. 36 ◽  
Author(s):  
Dini Siswani Mulia ◽  
Rachmansyah Rachmansyah ◽  
Triyanto Triyanto
Keyword(s):  
Survival Rate ◽  
Antibody Titer ◽  
Aeromonas Hydrophila ◽  
Challenge Test ◽  
Cell Debris ◽  
Oral Vaccination ◽  
Booster Injection ◽  
Time To Death ◽  
And Control ◽  
Mean Time

The aims of this research were to evaluate the effect of booster on the efficacy of vaccination with cell debris of Aeromonas hydrophila. Catfish (Clarias sp.) (10-13 cm of total length) were used for experiment with 4 treatments, i.e. (1) OS: oral vaccination and injection booster; (2) OO: oral vaccination and oral booster; (3) OR: oral vaccination and immersion booster; (4) without vaccination and booster (control). Booster was conducted one week after vaccination. The challenge test was conducted two weeks after booster. The results showed that the survival rate, Relative Percent Survival (RPS), Mean Time to Death (MTD), and antibody titer of OS treatment was significantly different (P<0.05) with OO, OR, and control. The survival rate and RPS of fish vaccinated by OS treatment reached 100%. The highest antibody titer was 2048 for OS treatment. The result sugested that oral vaccination followed by booster injection was the most effective method of vaccination.

scholarly journals THE EFFECT OF GIVING ONION (Allium Sativum) EXTRACT TO TREAT THE SEED OF TILAPIA (Oreochromis Niloticus) BACTERIAL INFECTED Aeromonas hydrophila

2021 ◽  
Vol 1 (2) ◽  
pp. 80-87
Author(s):  
Ani Lestari ◽  
Nurliah ◽  
Baiq Hilda Astriana
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Aquatic Environment ◽  
Allium Sativum ◽  
Bacterial Resistance ◽  
Survival Rates ◽  
Garlic Extract ◽  
Percent Survival ◽  
Randomized Design ◽  
Prevention And Treatment

MAS disease (Motile Aeromonas Septicemia) is a disease caused by A. hydrophila bacteria that attack tilapia and other freshwater fish species. The use of antibotics and chemicals in the continuous prevention and treatment of this disease can damage the aquatic environment and can cause bacterial resistance to antibotics, so antibotic use becomes ineffective. The study used a Complete RandomIzed Design (RAL) with an experimental method consisting of 5 treatments and 3 repeats. Treatment in this study is (P1) without soaking garlic extract and without infected bacteria Aeromonas hydrophila, (P2) without soaking garlic extract and infected bacteria Aeromonas hydrophila, (P3) soaking garlic extract with a concentration of 1% and disinfection of bacteria Aeromonas hydrophila, (P4) immersion of garlic extract with a concentration of 1.5% and infected bacteria Aeromonas hydrophila, (P5) soaked garlic extract with a concentration of 2% and infected with Aeromonas hydrophila bacteria. The results showed that the SR value of tilapia fish in each treatment had values ranging from 0.0% to 66.7%. Relative percent survival (RPS) tilapia seeds in each treatment have values ranging from 0.0% to 66.7%. The conclusion of this study is that giving garlic extract with different concentrations has a real different influence on survival rates and relative percent survival (RPS).

scholarly journals Individual and flock immunity responses of naïve ducks on smallholder farms after vaccination with H5N1 Avian Influenza vaccine: a study in a province of the Mekong Delta, Vietnam

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6268
Author(s):  
Hoa Thi Thanh Huynh ◽  
Liem Tan Truong ◽  
Tongkorn Meeyam ◽  
Hien Thanh Le ◽  
Veerasak Punyapornwithaya
Keyword(s):  
Avian Influenza ◽  
Influenza Vaccine ◽  
Prevention And Control ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Vaccination Program ◽  
Smallholder Farms ◽  
H5n1 Avian Influenza ◽  
Antibody Titers ◽  
And Control

In Vietnam, vaccination has played a crucial role in the national strategy for the prevention and control of H5 highly pathogenic avian influenza (HPAI). This study aimed to evaluate antibody responses of immunologically naïve domestic ducks to H5N1 avian influenza vaccine currently used in the national mass vaccination program of Vietnam. Blood samples of 166 ducks reared on smallholder farms were individually collected at three sampling time points, namely, right before vaccination, 21 days after primary vaccination, and 21 days after booster vaccination. Vaccine-induced antibody titers of duck sera were measured by the hemagglutination inhibition assay. Temporal differences in mean antibody titers were analyzed using the generalized least-squares method. No sampled ducks showed anti-H5 seropositivity pre-vaccination. The geometric mean titer (GMT) of the vaccinated ducks was 5.30 after primary vaccination, with 80% of the vaccinated ducks showing seropositivity. This result indicates that the immunity of duck flocks met the targets of the national poultry H5N1 HPAI mass vaccination program. GMT and seropositive rates of the ducks were 6.48 and 96.3%, respectively, after booster vaccination, which were significantly higher than those after primary vaccination. Flock-level seroprotection rate significantly increased from 68% to 84.7%, whereas variability in GMT titers decreased from 34.87% to 26.3%. This study provided important information on humoral immune responses of ducks to the currently used H5N1 vaccine under field conditions. Our findings may help guide veterinary authorities in planning effective vaccine protocols for the prevention and control of H5N1 in the target poultry population.

scholarly journals Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)

2017 ◽  
Vol 16 (1) ◽  
pp. 92 ◽  
Author(s):  
, Sukenda ◽  
Kiki Amalia Pratiwi ◽  
, Rahman ◽  
Dendi Hidayatullah
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Passive Immunization ◽  
Relative Survival ◽  
Control Fish ◽  
Cell Vaccine ◽  
Relative Survival Rate ◽  
Whole Cell Vaccine ◽  
Antibody Titers ◽  
And Control

<p class="Pa3"><strong>ABSTRACT </strong></p><p> </p><p class="Pa5">Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of <em>Aeromonas hydrophila </em>vaccine on the catfish broodstock to maternal transfer of immunity, and offspring resistance. The average body weight of broodstock used in this study were 650±50 g were kept in pool tarps sized 2×1×0.5 m3. This study used a randomized complete design with two treatments and three replications. Female broodstock were vaccinated using intraperitonial injections at a dose 0.4 mL/kg and control fish were injected with phospate buffered saline (PBS). The observed parameters include hematology of broodstock, mortality, the relative survival rate, and antibody titers. Antibody titer measurements on broodstock, eggs, and offspring. Vaccination on broodstock catfish delivers a significant antibody level (P&lt;0.05) on offspring compared to control catfish with relative survival rate of offspring at 5, 10, and 15 days after hatching were 67.76%, 82.66%, and 71.66% respectively.</p><p> </p><p class="Pa5">Keywords: catfish, <em>Aeromonas hydrophila</em>, vaccination, antibody transfer</p><p> </p><p> </p><p class="Pa3"><strong>ABSTRAK </strong></p><p> </p><p class="Pa5">Transfer kekebalan dari induk kepada benih melalui imunisasi pasif merupakan salah satu cara untuk memberikan proteksi pada benih. Tujuan penelitian ini untuk menguji efikasi vaksin sel utuh <em>Aeromonas hydrophila </em>pada induk ikan nila dalam mentransfer kekebalan spesifik ke benih dan menguji ketahanan benih hasil pemijahan induk yang divaksin. Induk lele yang digunakan pada penelitian ini memiliki bobot rata-rata 650±50 g dipelihara di kolam terpal berukuran 2×1×0,5 m3. Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Induk betina lele divaksinasi secara intraperitonial dengan dosis 0,4 mL/kg ikan dan induk lele kontrol disuntik dengan phospate buffer saline (PBS). Parameter yang diamati meliputi hematologi induk, mortalitas, tingkat kelangsungan hidup relatif benih, dan titer antibodi. Vaksinasi induk lele memberikan hasil level antibodi yang signifikan (P&lt;0,05) pada induk, telur, dan benih lele dibandingkan perlakuan kontrol dengan tingkat kelangsungan hidup relatif benih umur 5, 10, dan 15 hari pacatetas masing-masing sebesar 67,76; 82,66%; dan 71,66%.</p><p> </p><p>Kata kunci: ikan lele, <em>Aeromonas hydrophila</em>, vaksinasi, transfer antibodi</p>

Response of Rainbow Trout (Salmo gairdneri) to Antigens of Aeromonas hydrophila

1966 ◽  
Vol 23 (10) ◽  
pp. 1487-1494 ◽  
Author(s):  
George Post
Keyword(s):  
Rainbow Trout ◽  
Antibody Titer ◽  
Aeromonas Hydrophila ◽  
Synthesis Method ◽  
Phagocytic Index ◽  
Salmo Gairdneri ◽  
Antibody Synthesis ◽  
Humoral Antibody ◽  
Antibody Titers ◽  
Immunization Procedure

Rainbow trout (Salmo gairdneri) produced antibodies specific for Aeromonas hydrophila. Two parenteral routes yielded no measurable difference in rate of antibody synthesis. Method of bacterin suspension indicated adjuvant suspension was superior in maintenance of humoral antibody and challenge protection when compared to saline suspension of the organism.Antibody synthesis from adjuvant-suspended bacterin lagged approximately 30 days behind the rate of synthesis by fish receiving saline suspended bacteria. The highest measurable antibody titer reached by an individual was 1:64. Individuals receiving saline suspended bacterin showed evidence of loss of titer approximately 2 months after the last antigen inoculation. Definite decrease in titer was noted 5 months after the last antigen inoculation and the return to a probable nonimmune class by approximately one-half of the individuals 7 months after the last inoculation. All individuals receiving adjuvant-suspended bacterin remained in the probable immune class for as long as 6 months following the last bacterin inoculation, but only approximately one-half of the individuals remained in the probable immune class 7 months postinoculation.All fish receiving a weighed quantity of killed A. hydrophila orally each day for 9 consecutive months showed presence of humoral A. hydrophila antibody. This immunization procedure produced 50% of the individuals with humoral antibody titers considered to be in the probable immune class.Fish receiving no immunization remained with negative antibody titers throughout the experimental period.Definite protection was given fish which had been immunized by parenteral inoculation.Similar protection was given after immunization by either intramuscular or intraperitoneal routes. Fish receiving either saline or adjuvant-suspended bacterin parenterally showed analogous protection when challenged by the specific bacteria. Fish receiving oral bacterin received questionable protection against parenteral challenge of the specific organism.Phagocytosis was found to occur to a greater extent in immunized rainbow trout than in nonimmunized rainbow trout. An average phagocytic index of parenterally immunized rainbow trout was 1.08. Nonimmunized rainbow trout of the same age and egg source had an average phagocytic index of 0.30. The opsonic index was 3.6.

scholarly journals Immunogenicity of a commercial Salmonella enteritidis vaccine in layer birds

2019 ◽  
Author(s):  
F. Nasrin ◽  
M. S. R. Khan ◽  
M. A. Islam
Keyword(s):  
Immune Response ◽  
Antibody Titer ◽  
Salmonella Enteritidis ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Vaccination Schedule ◽  
Post Vaccination ◽  
Layer Chicken ◽  
Antibody Titers ◽  
Group A

Background: The aged birds are known to induce good immunity against Salmonella enteritidisas compared to young. To judge this hypothesis layer birds at 42 and 49 days old were vaccinated with AVI Pro®109SE4 vaccine and immune response in terms of antibody titers was measured. Methods: A composition of antibody production in vaccinated chicken was performed following a usual vaccination schedule with a newly suggested vaccination schedule. To study the immunogenicity of vaccine a total of 15 chickenswere divided into three groups. Each group comprised of 5 layer chicken. Chicken in group A and B were vaccinated with AVI Pro®109SE4 vaccine with a dose of 0.5ml/bird through SC route. Primary vaccination was performed at 42 days and 49 days of age respectively and booster vaccination was given at 72 days and 79 days of age respectively. Blood samples were collected to obtain sera from each chicken at every 7 days interval up to 93 days post vaccination for the determination of antibody titer using microplate agglutination test. Results: Highest mean antibody titers were recorded as179.20±70.11and 307.20±114.49 in birds of group A and B respectively. The highest mean antibody titer was recorded as 307.20±114.49 in chicken at 21 days post vaccination with AVI Pro®109SE4 vaccine using newly suggested schedule as compared to usual schedule of vaccination. Conclusions: Primary vaccination at birds at 49 days (newly planned vaccination schedule) of age induced better immune response as compared to birds vaccinated at 42 days of age.

scholarly journals THE EFFECT OF ONION (Allium sativum) EXTRACT TO TREAT TILAPIA FRY (Oreochromis niloticus) WITH BACTERIAL INFECTED Aeromonas hydrophila

2021 ◽  
Vol 1 (1) ◽  
pp. 29-39
Author(s):  
Ani Lestari ◽  
Nurliah ◽  
Baiq Hilda Astriana
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Allium Sativum ◽  
Clinical Symptoms ◽  
Survival Rates ◽  
Garlic Extract ◽  
Study Program ◽  
Percent Survival ◽  
Randomized Design ◽  
Completely Randomized Design

Research on the effect of garlic extract (allium sativum) to treat tilapia (oreochromis niloticus) seeds which are infected with aeromonas hydrophila bacteria. This research was conducted for 14 days from 18 December to 31 December 2019 which took place at the Wet Laboratory of the Aquaculture Study Program, Mataram University, West Nusa Tenggara (NTB). This study used a completely randomized design (CRD) with an experimental method consisting of 5 treatments and 3 replications. The container used in this study was a 15 liter jar with a volume of 10 liters. The containers used are 15 units, where in treatment one (P1) using without soaking garlic extract and without being infected with Aeromonas hydrophila bacteria, treatment two (P2) without soaking garlic extract and being infected with Aeromonas hydrophila bacteria, (P3) using soaking onion extract white with a concentration of 1% and infected with Aeromonas hydrophila bacteria, (P4) using garlic extract immersion with a concentration of 1.5% and infected with Aeromonas hydrophila bacteria, (P5) using garlic extract immersion with a concentration of 2% and infected with Aeromonas hydrophila bacteria. The parameters observed during the study included survival rate (SR), relative percent survival (RPS), observation of clinical symptoms, and water quality. The results showed that giving garlic extract with different concentrations had a significantly different effect on survival rates and relative percent survival (RPS).

scholarly journals PENGARUH PEMBERIAN IMUNOMODULATOR JINTAN HITAM (Nigella sativa L.) DI DALAM AIR MINUM TERHADAP TITTER ANTIBODI AVIAN INFLUENZA (AI) DAN NEWCASTLE DISEASE (ND) PADA BROILER BETINA

2020 ◽  
Vol 4 (3) ◽  
pp. 171-187
Author(s):  
Neni Kikiyani, Siswanto, Purnama Edy Santosa, dan Madi Hartono
Keyword(s):  
Drinking Water ◽  
Avian Influenza ◽  
Antibody Titer ◽  
Newcastle Disease ◽  
Nigella Sativa ◽  
Randomized Design ◽  
Antibody Titers ◽  
Disease Antibody ◽  
And Control ◽  
Different Doses

The aim of this research was to know the effectiveness of Nigella sativa L. as an immunomodulator given in drinking water with different doses on AI and ND antibody titers in female broilers. This research was conducted in December 2019 -- January 2020 at the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. The antibody titers was analyzed at the Lampung Veteriner Virology Laboratory in Lampung. This study used a Completely Randomized Design (CRD) with four treatments and three replications; namely drinking water without Nigella sativa L. (control) (P0), drinking water with 36 mg/kg BW/day Nigella sativa L., drinking water with 72 mg/kg BW/day Nigella sativa L., and drinking water with 144 mg/kg BW/day Nigella sativa L. The antibody titer data from each treatment and control was arranged in the form of a histogram and simple tabulations and then were analyzed descriptively. The results of this study indicated that female broilers given Nigella sativa L. was effective in increasing Newcastle Disease antibody titers and was not effective in increasing Avian Influenza antibody titers in female broilers. Giving Nigella sativa L. As much as 36 mg/kg BW/day in drinking water could increase the Newcastle Disease antibody titer in female broilers. Keywords: Antibody titer, Avian Influenza, Female broilers, Immunomodulator, Newcastle Disease, Nigella sativa L.

scholarly journals Comparative immune responses of pups following modified live virus vaccinations against canine parvovirus

2019 ◽  
Vol 12 (9) ◽  
pp. 1422-1427
Author(s):  
Jayalakshmi Vasu ◽  
Mouttou Vivek Srinivas ◽  
Prabhakar Xavier Antony ◽  
Jacob Thanislass ◽  
Vijayalakshmi Padmanaban ◽  
...  
Keyword(s):  
Immune Responses ◽  
Antibody Titer ◽  
Neutralizing Antibodies ◽  
Geometric Mean ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Canine Parvovirus ◽  
Serum Samples ◽  
Live Virus ◽  
Antibody Titers

Background and Aim: Canine parvovirus (CPV) is the most important viral cause of enteritis and mortality in pups. Evaluation and monitoring of pre- and post-vaccine immune responses may help to determine the efficacy of the current vaccination schedule being followed in pups in India. This study aimed to evaluate and monitor the pre- and post-vaccine immune responses of CPV vaccinated pups using hemagglutination inhibition (HI) assay. The neutralizing antibody titer levels were also detected using serum neutralization test (SNT). Materials and Methods: The pups were categorized into two groups, the double booster and the single booster groups. In this study, serum samples were subjected to HI and SNT for measuring the CPV antibody titer at frequent intervals for up to 6 months from 27 healthy pups following primary and booster CPV vaccinations. Results: The antibody titers in double booster pups reached their peaks at the 21st day after the second booster vaccination with a geometric mean (GM) of 3.57. The antibody titers in single booster pups reached their peaks at the 21st day after the first booster vaccination with a lower GM of 3.18. Conclusion: The double booster pups maintained a higher immune response throughout the period of the study compared to single booster pups though the difference in titers was not statistically significant. SNT results indicated that the raised antibody titer was also able to yield virus-neutralizing antibodies. No interfering maternally derived antibodies were found in the pups at the age of primary vaccination (45th day) in our study. Therefore, the second booster vaccination may be useful in maintaining the protective titer for a prolonged period.

scholarly journals Metarhizium brunneum Petch Fungi is Effective as a Bioinsecticide Against Coptotermes curvignathus Holmgren Termite Pests in the Laboratory

Elkawnie ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 259
Author(s):  
Muhammad Sayuthi ◽  
Teguh Santoso ◽  
Iswadi Iswadi
Keyword(s):  
Economic Value ◽  
Observation Time ◽  
Distilled Water ◽  
Time Of Death ◽  
Randomized Design ◽  
The Mean ◽  
Completely Randomized Design ◽  
And Control ◽  
Mean Time ◽  
Density Treatment

Abstract: Nutmeg is a plantation crop and has high economic value. Currently, the cultivation of nutmeg is experiencing many obstacles. One of them is termite pests. So far, the control is carried out using chemical insecticides and hurts the environment. Therefore it is necessary to control other environmentally friendly ways, and one of them is by using the fungi M. brunneum as an ecologically friendly bioinsecticide. The purpose of this study was to analyze the effectiveness of M. brunneum as a bioinsecticide against the termite C. curvignathus as an essential pest in nutmeg plantations. This research using a completely randomized design (CRD). The results showed that the mean percentage viability of M. brunneum conidia at each observation time was classified as useful. The germination of conidia increased to 89.78 % after 72 hours of observation. Conidia density 103/ mL distilled water. The highest mortality rate of C. curvignathus was found at a density of 1010 conidia/ mL of distilled water. The mean time of death for C. curvignathus after application of conidia density treatment of 1010/ mL of distilled water was 2.15 days and the death of C. curvignathus was 3.35 days using conidia density treatment 103/ mL of distilled water. 1 day after application reaches 33.20 % and increases to observation at 5 days after application reaches 100 %. The highest food inhibition occurred in treatment 1010/ mL distilled water (65.81 %) which was significantly different from treatment 105/ mL distilled water (43.23 %), and 103/ mL distilled water (41.61 %) and control (0.00).Abstrak: Pala merupakan tanaman perkebunan dan bernilai ekonomi tinggi. Pada saat ini budidayanya mengalami banyak hambatan. Satu diantaranya adalah serangan hama rayap. Selama ini  pengendalian hama rayap dilakukan dengan menggunakan insektisida kimia dan berpengaruh negatif terhadap lingkungan. Oleh karenanya perlu pengendalian cara lain yang ramah lingkungan dan satu diantaranya dengan menggunakan cendawan M. brunneum sebagai bioinsektisida yang ramah lingkungan. Tujuan dari penelitian ini adalah menganalisis efektivitas M. brunneum sebagai bioinsektisida terhadap hama rayap C. curvignathus. Penelitian ini dilaksanakan di Laboratorium Hama Tanaman dan Laboratorium Dasar Proteksi Tanaman Program Studi Proteksi Tanaman Fakultas Pertanian Universitas Syiah Kuala, sejak bulan Februari hingga September 2018, dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa persentase viabilitas rata-rata dari konidia M. brunneum pada setiap waktu pengamatan tergolong efektif. Perkecambahan konidia semakin meningkat hingga mencapai 89,78 % setelah diamati pada jam yang ke 72. Rata-rata waktu kematian rayap C. curvignathus setelah aplikasi perlakuan kerapatan konidia 1010/ mL akuades adalah 2,15 hari dan terjadi kematian rayap C. curvignathus 3.35 hari dengan menggunakan perlakuan kerapatan konidia 103/ mL akuades. Rata-rata mortalitas tertinggi rayap C. curvignathus ditemukan pada perlakuan kerapatan konidia 1010/mL aquades. 1 HSA mencapai 33,20% dan meningkat hingga pengamatan pada 5 HSA mencapai 100%. Daya hambat makan tertinggi terjadi pada perlakuan 1010/ mL aquades (65,81%) yang berbeda nyata dengan perlakuan 105/mL aquades (43,23%) dan 103/ mL aquades (41,61%) serta kontrol (0,00%).

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