scholarly journals Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides

2007 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
F. Johnny ◽  
A. Priyono ◽  
D. Roza
Keyword(s):  
Body Weight ◽  
Sea Water ◽  
Hormone Treatment ◽  
Epinephelus Coioides ◽  
Male Ratio ◽  
Concrete Tank ◽  
Orange Spot ◽  
Research Institute

<p>An experiment to study the influence of the chronic hormone LHRH-a and 17a-MT on haematology orange spot grouper, Epinephelus coioides broodstock was conducted at Gondol Research Institute for Mariculture, Bali.  Fish weights were ranged 4.7-10.4 kg (female) and 10.4-17.8 kg (male).  The females broodstock was treated by hormone 17a-MT with dosage 50 mg/kg body weight, and males was by hormone LHRH-a with dosage 50 and 100 mg/kg body weight.  Fish were kept on two concrete tank contained 100 m<sup>3</sup> sea water and 2 meters depth and density 15 fishes/tank,  male ratio and female was 1 : 2.  Hormone treatment was peformed every two months until six months culture.  Blood were collected before treatment by hormone and every two months.  Hemocytology parameters measured were hematocrit, hemoglobin, total erythrocyte, and total leucocyte.  Result of the average indicator of hemacytology to influence on haemological parameters.</p> <p>Keywords:  Epinephelus coioides, chronic hormone, hemocytology, orange spot grouper broodstock.</p> <p> </p> <h2>ABSTRAK</h2> <p>Suatu percobaan dengan tujuan untuk mengetahui pengaruh  hormon LHRH-a dan 17a-MT terhadap keragaan hemositologi induk ikan kerapu lumpur, <em>Epinephelus coioides</em> telah dilakukan di Balai Besar Riset Perikanan Budidaya Laut Gondol, Bali.  Ikan uji yang digunakan adalah induk ikan kerapu lumpur kelamin betina dengan bobot antara 4,7-10,4 kg dan induk kelamin jantan dengan bobot antara 10,4-17,8 kg.  Ikan uji dipelihara dalam bak beton volume 100 m<sup>3</sup> dengan kepadatan 15 ekor/bak, dilengkapi aerasi dan pergantian air sistim mengalir.  Induk kelamin betina diberikan perlakuan implantasi pelet hormon LHRH-a dosis 50 mg/kg bobot, dan induk kelamin jantan dengan hormon 17a-MT dosis 50 dan 100 mg/kg bobot ikan.  Implantasi dilakukan setiap 2 bulan, pada awal percobaan (sebelum perlakuan hormon/bulan ke-0), bulan ke-2, bulan ke-4, dan bulan ke-6 dilakukan sampling darah untuk pengujian keragaan hemositologi, meliputi; persentase hematokrit, kadar nilai hemoglobin, jumlah total eritrosit, dan jumlah total leukosit.   Hasil percobaan menunjukkan bahwa penggunaan hormon LHRH-a pada induk ikan kerapu lumpur kelamin betina dan hormon 17a-MT pada induk ikan kerapu lumpur kelamin jantan berpengaruh terhadap keragaan hemositologi.</p> <p>Kata kunci:  <em>Epinephelus coioides, </em> hemositologi, hormon kronik, induk ikan kerapu lumpur</p>

IMPLANTATION OF LHRH-a HORMONE TO STIMULATION OF GONAD DEVELOPMENT OF HUMPBACK GROUPER SECOND GENERATION ( F-2 )

2013 ◽  
Vol 5 (2) ◽  
Author(s):  
Tridjoko Tridjoko
Keyword(s):  
Body Weight ◽  
Second Generation ◽  
Gonad Development ◽  
Gonadal Development ◽  
Oocyte Diameter ◽  
2Nd Generation ◽  
Length Range ◽  
Concrete Tank ◽  
Implant Treatment ◽  
Stimulation Of

This study aimed to determine the effect of LHRH-a hormone implantation on gonadal development of humpback grouper, Cromileptes altivelis 2nd generation (F-2). Individuals used were 20 female grouper fish with weight range of 600-800 gram/fish and total length range of 28.0-30.0 cm. The treatment used 5 fish each with tagging. Grouper fishes were stocked into the 75 m3 circular concrete tank. Hormone of LHRH-a with dosage of 50 μg/kg body weight was used as implant treatment of (A) 1 time,, (B) 2 times, (C) 3 times, and (D) without implant. The results showed that implantation of LHRH-a hormone were significantly stimulate gonad development of humpback grouper second generation (F-2). Treatment of 3 times implant every month showed the best results produced  oocyte diameter up to 480 μm, while the control of oocyte diameter only reached <400 μm. Keywords: LHRH-a hormone, implantation, Humpback grouper, gonad development

The effects of lowered salinity upon Branchiostoma lanceolatum from the English Channel

1981 ◽  
Vol 61 (3) ◽  
pp. 685-689 ◽  
Author(s):  
J. Binyon
Keyword(s):  
Body Weight ◽  
Osmotic Stress ◽  
Sea Water ◽  
English Channel ◽  
Sharp Threshold ◽  
Branchiostoma Lanceolatum

Previous work has indicated that, although in possession of numerous supposedly osmoregulatory structures, Branchiostoma lanceolatum from the English Channel is unable to regulate its body weight in diluted sea water. In vitro measurements of the rate of flagella activity in those organs similarly indicates no increase under hypo-osmotic stress. There is however quite a sharp threshold around 18‰, below which flagella activity ceases quite abruptly. A similar situation obtains with the gill cilia although the animal can remain alive at these salinities for a period of several weeks.

Urinary Bladder Volume and the Reabsorption of Water from the Urine of Crabs

1974 ◽  
Vol 60 (1) ◽  
pp. 167-181
Author(s):  
J. A. RIEGEL ◽  
A. P. M. LOCKWOOD ◽  
J. R. W. NORFOLK ◽  
N. C. BULLEID ◽  
P. A. TAYLOR
Keyword(s):  
Body Weight ◽  
Urinary Bladder ◽  
Blood Volume ◽  
Ethacrynic Acid ◽  
Sea Water ◽  
Carcinus Maenas ◽  
Bladder Volume ◽  
Active Secretion ◽  
51Cr Edta

1. Measurements have been made to determine the blood volume, bladder volume, clearance of 131I-sodium diatrizoate and U/H for diatrizoate in the crabs Carcinus maenas and Macropipus (Portunus) depurator. 2. Observed values of clearance blood volume and bladder volume in the two species at 18 °C were: Clearance (as % blood volume per day), Macropipus 56.1±14.5; Carcinus 27.1±5.8; Blood volume (as % body weight), Macropipus 21.0±4.0; Carcinus 19.2±3.0; Bladder volume (as % blood volume), Macropipus 12.1 ±5.0; Carcinus 11.0±8.0. 3. It is shown that the measured U/H differs from that to be expected if no reabsorption of water or secretion of diatrizoate occurs. 4. 14C-inulin and 51Cr-EDTA are excreted in an essentially similar manner to 131I-diatrizoate by Carcinus, implying that any active secretion of diatrizoate must be small in magnitude. 5. Injections of ethacrynic acid decrease the U/H ratio for diatrizoate relative to that in control Carcinus injected with sea water. In some Carcinus the concentration of diatrizoate in the urine comes to exceed that initially present in the blood. Both these points are taken, with 3, as support for the conclusion that water can be withdrawn from the primary urine of Carcinus.

Sodium Regulation and Adaptation to Fresh Water in Gammarid Crustaceans

1968 ◽  
Vol 48 (2) ◽  
pp. 359-380
Author(s):  
D. W. SUTCLIFFE
Keyword(s):  
Body Weight ◽  
Fresh Water ◽  
Body Surface ◽  
Sea Water ◽  
Sodium Concentration ◽  
The Body ◽  
Outward Diffusion ◽  
Gammarus Zaddachi ◽  
Freshwater Habitats ◽  
Sodium Regulation

1. Sodium uptake and loss rates are given for three gammarids acclimatized to media ranging from fresh water to undiluted sea water. 2. In Gammarus zaddachi and G. tigrinus the sodium transporting system at the body surface is half-saturated at an external concentration of about 1 mM/l. and fully saturated at about 10 mM/l. sodium. In Marinogammarus finmarchicus the respective concentrations are six to ten times higher. 3. M. finmarchicus is more permeable to water and salts than G. zaddachi and G. tigrinus. Estimated urine flow rates were equivalent to 6.5% body weight/hr./ osmole gradient at 10°C. in M. finmarchicus and 2.8% body weight/hr./osmole gradient in G. zaddachi. The permeability of the body surface to outward diffusion of sodium was four times higher in M. finmarchicus, but sodium losses across the body surface represent at least 50% of the total losses in both M. finmarchicus and G. zaddachi. 4. Calculations suggest that G. zaddachi produces urine slightly hypotonic to the blood when acclimatized to the range 20% down to 2% sea water. In fresh water the urine sodium concentration is reduced to a very low level. 5. The process of adaptation to fresh water in gammarid crustaceans is illustrated with reference to a series of species from marine, brackish and freshwater habitats.

scholarly journals DIFFERENCES IN CARCASS PROPORTIONS AND COMPOSITION IN CONTROL AND HORMONE-TREATED HOLSTEIN-FRIESIAN STEERS AND BULLS

1978 ◽  
Vol 58 (3) ◽  
pp. 333-338 ◽  
Author(s):  
R. J. FORREST
Keyword(s):  
Body Weight ◽  
Hormone Treatment ◽  
Fat Deposition ◽  
Carcass Composition ◽  
Holstein Friesian

The carcass proportions and fat deposition were studied in 72 Holstein–Friesian males reared on an all-concentrate ration and slaughtered at 475 kg. One half were castrated at 136 kg body weight and 18 bulls and 18 steers were implanted with 200 mg progesterone plus 20 mg estradiol-17-β-benzoate at 340 kg. There was no evidence of any difference between steers and bulls nor of any effect of the hormone treatment for the tail, butt, tip, round or front shank portions of the carcass. Steers had proportionately more flank, short loin, brisket and back and less chuck than bulls. With hormone treatment the percentage of rib and belly decreased and the hind, rump and rear shank increased in steers. With bulls, implanting decreased the rear shank and hind and increased the belly section. In carcass composition the bulls had a higher percentage of bone than steers. Implanting decreased the fat and increased the lean percentage in steers but did not significantly affect the bulls.

The Water Balance of a Serpulid Polychaete, Mercierella Enigmatica (Fauvel)

1974 ◽  
Vol 60 (2) ◽  
pp. 321-330
Author(s):  
HELEN LE B. SKAER
Keyword(s):  
Body Weight ◽  
Water Balance ◽  
Blood Volume ◽  
Blood Concentration ◽  
Volume Regulation ◽  
Sea Water ◽  
External Medium ◽  
Wide Range ◽  
Natural Range ◽  
Passive Movements

1. The serpulid polychaete Mercierella enigmatica is found naturally in a wide range of salinities - from fresh water to 150% sea water (&lt; 1-55‰ &lt; 25.8-1421 mOsm). 2. Changes in body weight, blood volume and blood osmolality have been measured both during and after equilibration of animals with media of altered salinity. 3. The blood remains similar in osmolality to the external medium over a very wide range of salinity (43-1620 mOsm); osmoregulation occurs only at the lowest limit of the natural range. 4. Mercierella enigmatica shows volume regulation; after 4 days of equilibration with a medium of altered salinity the blood volume shows much less change than the blood concentration. 5. During equilibration there appear to be passive movements of both water and salts between the animals and their environment.

Effect of body weight and sex on activity of enzymes involved in amino acid metabolism

1959 ◽  
Vol 196 (2) ◽  
pp. 303-306 ◽  
Author(s):  
Edmund C. C. Lin ◽  
Richard S. Rivlin ◽  
W. Eugene Knox
Keyword(s):  
Body Weight ◽  
Amino Acid ◽  
Sex Difference ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
General Rule ◽  
Hormone Treatment ◽  
Oxygen Utilization ◽  
Body Weights ◽  
Total Body

The total body activities of four transaminases and two oxidases concerned with aromatic amino acid metabolism were studied in five species of mammals of varying size to test for correlations between total body enzyme activity and body weight. It was found that as a general rule the following expression was obeyed: enzyme x weight k = constant (the weight parity expression), where k varied numerically from –0.25 to –0.73 depending on the enzyme. The two oxidases had values which varied from –0.71 to –0.73 which are very close to the value –0.73 established for total oxygen utilization as a function of body weight. The use of the weight parity expression permitted the analysis within a given species of enzyme activities in animals with different body weights. Such an analysis proved useful in distinguishing an enzyme, tyrosine-α-ketoglutarate transaminase, whose level showed a true sex difference from others whose levels showed apparent sex differences which were actually referable to the smaller body weights of the females. Direct experimentation involving castration and hormone treatment substantiated this conclusion and identified testosterone as the important controlling factor. This sex difference was explicable by the known effects of adrenal cortical hormones on the level of this enzyme and by the reported differences in the rate of hepatic metabolism of adrenal cortical hormone in male and female animals.

Seasonal Variation in Physiological Energetics of Mytilus Edulis and Cerastoderma Edule of Different Size Classes

1997 ◽  
Vol 77 (3) ◽  
pp. 817-838 ◽  
Author(s):  
A.C. Smaal ◽  
A.P.M.A. Vonck ◽  
M. Bakker
Keyword(s):  
Body Weight ◽  
Seasonal Variation ◽  
Mytilus Edulis ◽  
Clearance Rate ◽  
Sea Water ◽  
Stepwise Multiple Regression ◽  
Reproductive Condition ◽  
Cerastoderma Edule ◽  
Size Classes ◽  
Respiration Rates

Seasonal variation of clearance, absorption and respiration rates of mussels Mytilus edulis (Mollusca: Bivalvia) and cockles Cerastoderma edule (Mollusca: Bivalvia) of various size classes have been measured under ambient temperature and sea-water conditions. Food availability was kept almost constant during the measurements.Allometric body weight coefficients were bcl=0.5 for clearance and about br=0.7 for respiration rate of both species. Stepwise multiple regression suggested no relation for clearance rate of mussels with temperature and reproductive condition throughout the year. Respiration rates of mussels were highest during the reproductive period. Excretion rate was measured for mussels only and showed an allometric coefficient of bex=0.7 and an increase with temperature in spring. For cockles a significant relation of clearance rate, body weight and temperature was observed; at low temperature, clearance rates of small animals were more reduced than for larger animals. Respiration rates of cockles showed a significant relationship with temperature and reproductive condition.

THE INFLUENCE OF GROWTH HORMONE TREATMENT ON THYROID FUNCTION IN SWINE

1990 ◽  
Vol 70 (3) ◽  
pp. 991-995 ◽  
Author(s):  
R. N. KIRKWOOD ◽  
P. A. THACKER ◽  
B. LAARVELD
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Key Words ◽  
Growth Hormone Treatment ◽  
Sampling Period ◽  
Hormone Treatment ◽  
Serum Concentrations ◽  
Significant Treatment ◽  
Blood Samples ◽  
To Receive

Twelve castrated male pigs of Yorkshire and Landrace breeding were selected at 95.9 ± 1.6 kg body weight (BW) and allocated equally to receive daily injections of either porcine growth hormone (pGH) at 90 μg kg−1 BW or vehicle buffer for four consecutive days. Following the last pGH injection, the pigs were infused via indwelling vena caval cannulae with thyrotropin-releasing hormone (TRH) at 0.5 μg kg−1 BW. Blood samples were obtained at 10 and 0 min before TRH and thereafter at 10-min intervals for 90 min. Serum concentrations of thyroxine and thyrotropin were lower (P < 0.06 and P < 0.1, respectively) but those of triiodothyronine higher (P < 0.01) in pGH-treated pigs throughout the sampling period. There were no significant treatment-by-time interactions indicating that the thyroid response to TRH was not influenced by pGH treatment. Key words: Swine, thyroid, growth hormone, somatotropin

scholarly journals Profil hormon FSH, LH dan estradiol serta kadar glukosa darah sidat, Anguilla bicolor bicolor (Mc Clelland, 1844) yang dirangsang hormon HCG, MT, E2 dan anti dopamin

2018 ◽  
Vol 18 (1) ◽  
pp. 57
Author(s):  
Abdul Zahri ◽  
Agus Oman Sudrajat ◽  
Muhammad Zairin Junior
Keyword(s):  
Blood Glucose ◽  
Sea Water ◽  
Gonadal Development ◽  
Permutation Patterns ◽  
Exogenous Hormone ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Randomized Design ◽  
Fish Blood ◽  
Concrete Tank

This study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range. AbstrakThis study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range.

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