Determination of the quantitative content of some groups of phenolic compounds in tinctures from the raw material of plant families Polygonaceae, Rosaceae, Asteraceae

Aim. To determine the quantitative content of total polyphenols and the amount of hydroxycinnamic acids in the series of tinctures from rhizomes with roots of Rumex confertus Willd., Sanguisorba officinalis L., roots of Rosa majalis Herrm., Rosa canina L., Arctium lappa L., Arctium minus (Hill) Bernh., Arctium tomentosum Mill., and the herb of Bidens tripartita L.Materials and methods. As study objects the tinctures from rhizomes with roots of Rumex confertus, Sanguisorba officinalis, roots of Rosa majalis, Rosa canina, Arctium lappa, Arctium minus, Arctium tomentosum and the herb of Bidens tripartita were used. These tinctures were obtained by the method of maceration at room temperature and the ratio of 1 : 5 of the plant raw material/finished products; the extractant was 50 % ethyl alcohol. The quantitative content of total polyphenols and the amount of hydroxycinnamic acids was determined by spectrophotometry according to the methods of the State Pharmacopoeia of Ukraine.Results and discussion. The limits of the quantitative content of total polyphenols and the amount of hydroxycinnamic acids in the tinctures were determined. They were not less than 0.070 mg mL-1 and 0.002 mg mL-1 for the tincture of rhizomes with roots of Rumex confertus, 0.100 mg mL-1 and 0.005 mg mL-1 for the tincture of rhizomes with roots of Sanguisorba officinalis, 0.070 mg mL-1 and 0.002 mg mL-1 for the tincture of Rosa majalis roots, 0.080 mg mL-1 and 0.001 mg mL-1 for the tincture of Rosa canina roots, 0.01 mg mL-1 and 0.001 mg mL-1 for the tincture of Arctium lappa roots, 0.010 mg mL-1 and 0.002 mg mL-1 for the tincture of Arctium minus roots, 0.001 mg mL-1 and 0.002 mg mL-1 for the tincture of Arctium tomentosum roots, 0.070 mg mL-1 and 0.001 mg mL-1 for the tincture of Bidens tripartita herb, respectively.Conclusions. The quantitative content of total polyphenols and the amount of hydroxycinnamic acids in tinctures from rhizomes with roots of Rumex confertus, Sanguisorba officinalis, roots of Rosa majalis, Rosa canina, Arctium lappa, Arctium minus, Arctium tomentosum and the herb of Bidens tripartita have been determined. The data obtained will be used in further work on the study of tinctures of these types of the plant raw material.

The vulnerary potential of botanical medicines in the treatment of bacterial pathologies in fish

Background and Aim: The use of plant-based medicine in treating and preventing fish disease has become increasingly popular due to the resistance of bacterial pathogens to chemicals widely used in aquaculture. This study explored the vulnerary effect of botanical medicines made from local raw materials (greater celandine [Chelidonium majus L.], St. John's wort [Hypericum perforatum L.], and bur beggar-ticks [Bidens tripartita L.]) in the treatment of sturgeon bacterial pathologies in a controlled environment. Materials and Methods: The vulnerary activity of herbal infusions was studied on spontaneously infected fish by assessing the degree of wound healing at regular intervals: The state of the wound, reduction of the wound surface area, the formation of granulation tissue, epithelization, and wound contraction. Results: A positive vulnerary effect of C. majus, H. perforatum, and B. tripartita was observed, consistent with the use of these plants in folk and traditional medicine. The plant materials eliminated infection, had anti-inflammatory and vulnerary effects, stimulated granulation tissue development, and enhanced regeneration. Compared with widely accepted methods (antibiotics and other chemotherapeutic agents), botanical medicine facilitated more effective treatment over the same period without side effects. Conclusion: Practical use and the results of this study show the potential of using herbal infusions for therapeutic purposes in aquaculture.

THE STUDY OF THE EXTRACTION DYNAMICS OF BIOLOGICALLY ACTIVE SUBSTANCES FROM THE BIDENS TRIPARTITA L. HERB AND ANTIOXIDANT ACTIVITY OF THE OBTAINED EXTRACTS

The interest to study the genus Bidens and in particular Bidens tripartita L. (bur-marigold herb) is unmitigated due to its antioxidant, anti-allergic, antimicrobial, antifungal, hepatoprotective, immunostimulating and hypotensive activity. This pharmacological activities are determined by the presence of flavonoids, polyphenolic compounds, polysaccharides, components of essential oils, polyacetylenes, etc. The aim of the research was to study the process of extracting various biologically active substances (polysaccharides, flavonoids, polyphenols) from bur-marigold herb with water-alcohol mixtures of various concentrations, to study their component composition, as well as to evaluate their effect on the oxidative effect of free radicals. Materials and methods. The object of the research was the herb of Bidens tripartita L. Raw materials were collected and procured on the territory of Kharkiv, Zhytomyr and Poltava regions of Ukraine. All used methods for the quantitative determination of biologically active substances were pharmacopeial and described in various monographs in the European Pharmacopoeia and State Pharmacopoeia of Ukraine. Results. The extracts of the bur-marigold herb were analyzed for the content of the extractable matter, flavonoids, polysaccharides and it was found that they are maximally extracted (about 80 %) in the 1st percolate with a drug/solvent ratio of 1:10 for all the extractants. It was found that with an increase of ethanol concentration in the extraction mixture, the content of polyphenols and flavonoids, is expectedly increased, as well as the value of the antioxidant activity of the corresponding extracts, but the amount of extracted polysaccharides is significantly reduced. Conclusions. With the selected evaluation criteria, it was found that 40 % ethanol is optimal from the point of view of balanced extraction of flavonoids, polysaccharides and polyphenols. A strong positive and statistically significant correlation was found between the content of polyphenols in the obtained extracts and their antioxidant activity (Pearson correlation coefficient, r=0.9998), slightly weaker for flavonoids (r=0.9886), and an inverse correlation between polysaccharides content and TEAC- value.

Anatomical structure of the stem Bidens tripartita (Asteraceae) in connection with habitat

Internal structure of the stem in internodes of metamers middle and upper zones of inhibition, amplificationzone in generative individuals Bidens tripartita L. is described. It is of a transitional type, for a longer length – hollow.Epidermis with an anomocytic type of stomatal apparatus. Anatomical structure of the stem in plants from two communitieswas compared. Due to close biotope conditions, the biometric parameters of the structures do not differ.

Influence of ecological and geographical conditions on the content of phenolic compounds and ascorbic acid of medicinal plants

The aim of our study was to evaluate the dependence of the accumulation of phenolic compounds (soluble phenolic compounds and tannins) and ascorbic acid in different parts of three medicinal plants: Bidens tripartita, Urtica dioica and Chenopodium album on the place of growth. The plants were collected from three regions of Tatarstan (Vysokogorsky, Laishevsky and Spassky region. The results showed that the accumulation of these substances in the studied plants differs in different parts of the plants, depending on the place of growth. B. tripartita is the richest in the studied substances. Vysokogorsky and Spassky regions turned out to be areas with the best plant raw materials.

FLORA OF LAWNS IN THE CITY OF UFA

The paper presents a study in the flora of lawns in Ufa. The lawn flora is represented by 192 species belonging to 31 families. Based on coenotic and ecological characteristics, four ecological coenotic groups of species were singled out that were described in the lawn designated areas. The ecological coenotic groups were analysed for life form spectra, hemeroby, species activity. Among the plant communities of typical low-trampled and well-maintained urban lawns (the ecological coenotic group of Festuca pratensis) Poaceae, Asteraceae, Fabaceae, Rosaceae, Apiaceae families were the leading ones, where hemicryptophytes and oligo-meso-αeuhemerobes dominated. The lawns subjected to significant anthropogenic impact (trampling, salinization) (the ecological coenotic group of Taraxacum officinale) had a large number of species that belonged to the «ruderal» Polygonaceae, Asteraceae, Fabaceae, Brassicaceae, Lamiaceae families, where hemicryptophytes and meso-euhemerobes dominated. Ruderal species of Asteraceae, Poaceae, Amaranthaceae, Lamiaceae, Malvaceae, Polygonaceae families prevailed, with a large share of ruderal and semi-ruderal annual and biennial plants, β-eupolyhemerobes, in the lawn designated areas where gramineous plant community did not develop after soil disturbance (the ecological coenotic group of Chenopodium album). Ruderal species of Poaceae, Chenopodiaceae, Polygonaceae families with a large portion of ruderal annual and biennial β-eupolyhemerobes also dominated in humid and waterlogged lawn areas. The ecological ecoenotic groups of Taraxacum officinale, Chenopodium album and Bidens tripartita had a large number of neophyte species. The lawn areas with the ecological coenotic groups of Taraxacum officinale, Chenopodium album and Bidens tripartite were found as most colonized with ruderal plants. A number of measures are required to build dense grass covering: reconstruction and heavy maintenance of lawns combined with sowing quality lawn grass mixtures, regular mowing practices to reduce the number of ruderal hemytherophytes and therophytes, timely plant nutrition and watering.