scholarly journals Sintesis dan Uji Aktivitas Antibakteri Senyawa N-fenil-4-klorobenzamida

2018 ◽  
Vol 6 (2) ◽  
pp. 212
Author(s):  
Elok Dea Orens Ubung Wisnu ◽  
Indah Purnama Sary ◽  
Dwi Koko Pratoko
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Crystal Shape ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Benzamide Derivatives

N-phenylbenzamide is benzamide derivatives, which is potential as an antibacterial agent. N-phenyl-4-chlorobenzamide is N-phenylbenzamide derivative that substitued by chloro to the para position and it was expected to enhance the antibacterial activity. N-phenyl-4-chlorobenzamide was synthesized by reacting 1,3-diphenyilthiourea and 4-chlorobenzoil chloride. This compound has been purified and provided 53% of product with crystal shape, white color, and melting point of 195-197 oC. The purification of this compound was confirmed by TLC and the structure was identified by 1H-NMR, 13C-NMR, and FTIR spectroscopy. This compound was tested for its activity against gram positive bacteria Staphylococcus aureus and gram negative bacteria Pseudomonas aeruginosa, evaluated by well diffusion method and the result showed no activity against both Staphylococcus aureus and Pseudomonas aeruginosa.   Keywords: N-phenyl-4-chlorobenzamide, synthesis, antibacterial activity

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

Antimicrobial Evaluation and Efficient Green Synthesis of 8-Substituted-2,5-dihydro-1,5-benzothiazepine Derivatives

2019 ◽  
Vol 4 (1) ◽  
pp. 32-35
Author(s):  
Priyanka Sharma
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Gram Negative Bacteria ◽  
Aluminium Nitrate ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Hcl Gas ◽  
Antimicrobial Evaluation ◽  
Antimicrobial Assay

8-Substituted-2,5-dihydro-1,5-benzothiazepine derivatives have been synthesized by the reaction of 1-(2-furyl)-3-(3,4-dimethoxyphenyl)-2-propenone with six 5-substiuted-2-minobenzenethiols in dry ethanol saturated with dry HCl gas and also in the presence of aluminium nitrate as catalyst in dry ethanol. All the newly synthesized compounds were characterized by analytical and spectral data comprising IR, 1H NMR, 13C NMR, 19F NMR and mass studies. All these compounds have also been evaluated for their antimicrobial assay against the Gram-positive bacteria, Staphylococcus aureus and the Gram-negative bacteria, Pseudomonas aeruginosa and the fungus, Candida albicans. The antifungal activity was found to be more significant than antibacterial activity.

scholarly journals In-vitro analysis of potential antibacterial activity of three medicinal plants

2016 ◽  
Vol 8 (3) ◽  
pp. 1497-1500
Author(s):  
Vandana Gupta ◽  
Rakesh Kumar ◽  
Deepika Chaudhary ◽  
Nirmal Yadav
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

The present study was aimed to examine and compare the antibacterial activity of hot methanolic extract of medicinal plants viz. Portulaca oleracea (purslane), Syzygium cumini (L.) (jamun), Psidium guajava (L.) (guava). Antibacterial activity was carried by using agar well diffusion method, against Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). Results indicated that all the three plant extracts possess antibacterial property against Gram-positive bacteria and no activity was found against Gram-negative bacteria. Moderate zone of inhibition against Staphylococcus aureus and Bacillus subtilis was exhibited by S. cumini (L.) (11mm and 12mm) and P. guajava (L.) (10mm and 11mm) and weak zone of inhibition was exhibited by P. oleracea (5 mm and 6mm). In conclusion, S. cumini (L.) and P. guajava (L.) possess bettercapabilities of being a good candidate in search for natural antibacterial agent against infections and diseases causing Gram-positive bacteria as compared to P. oleracea.

Bioactive compounds isolated from submerged fermentations of the Chilean fungus Stereum rameale

2015 ◽  
Vol 70 (3-4) ◽  
pp. 97-102 ◽  
Author(s):  
Pedro Aqueveque ◽  
Carlos Leonardo Céspedes ◽  
José Becerra ◽  
Marcelo Dávila ◽  
Olov Sterner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bioactive Metabolites ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Bioassay Guided Fractionation

Abstract Liquid fermentations of the fungus Stereum rameale (N° 2511) yielded extracts with antibacterial activity. The antibacterial activity reached its peak after 216 h of stirring. Bioassay-guided fractionation methods were employed for the isolation of the bioactive metabolites. Three known compounds were identified: MS-3 (1), vibralactone (2) and vibralactone B (3). The three compounds showed antibacterial activity as a function of their concentration. Minimal bactericidal concentrations (MBC) of compound 1 against Gram-positive bacteria were as follows: Bacillus cereus (50 μg/mL), Bacillus subtilis (10 μg/mL) and Staphylococcus aureus (100 μg/mL). Compounds 2 and 3 were active only against Gram-negative bacteria. The MBC of compound 2 against Escherichia coli was 200 μg/mL. Compound 3 inhibited significantly the growth of E. coli and Pseudomonas aeruginosa, with MBC values of 50 and 100 μg/mL, respectively.

Antibacterial activity of magnesium oxide nanoparticles prepared by Calcination method

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Elaf Ayad Kadhem ◽  
Miaad Hamzah Zghair ◽  
Sarah , Hussam H. Tizkam, Shoeb Alahmad Salih Mahdi ◽  
Hussam H. Tizkam ◽  
Shoeb Alahmad
Keyword(s):  
Antibacterial Activity ◽  
Magnesium Oxide ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Magnesium Oxide Nanoparticles ◽  
Agar Disc

magnesium oxide nanoparticles (MgO NPs) were prepared by simple wet chemical method using different calcination temperatures. The prepared NPs were characterized by Electrostatic Discharge (ESD), Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD). It demonstrates sharp intensive peak with the increase of crystallinty and increase of the size with varying morphologies with respect to increase of calcination temperature. Antibacterial studies were done on gram negative bacteria (E.coli) and gram positive bacteria (S.aureus) by agar disc diffusion method. The zones of inhibitions were found larger for gram positive bacteria than gram negative bacteria, this mean, antibacterial MgO NPs activity more active on gram positive bacteria than gram negative bacteria because of the structural differences. It was found that antibacterial activity of MgO NPs was found it has directly proportional with their concentration.

scholarly journals Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Salu Rai ◽  
Uday Narayan Yadav ◽  
Narayan Dutt Pant ◽  
Jaya Krishna Yakha ◽  
Prem Prasad Tripathi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Gram Negative Bacteria ◽  
Wound Infections ◽  
Gram Negative ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Wound Swab ◽  
Susceptibility Patterns

In Nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. Total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique. Methicillin-resistantStaphylococcus aureusisolates were detected by using cefoxitin disc and confirmed by determining minimum inhibitory concentrations (MIC) of oxacillin. 264 (59%) samples were culture positive. The highest incidence of bacterial infections was noted in the age group of less than 1 year (76%). Out of 264 growth positive samples, Gram-positive bacteria were isolated from 162 (61%) samples and Gram-negative bacteria were found in 102 (39%) samples.Staphylococcus aureus(99%) was the predominant Gram-positive bacteria isolated andPseudomonas aeruginosa(44%) was predominant Gram-negative bacteria. About 19% ofS. aureusisolates were found to be methicillin-resistant MIC of oxacillin ranging from 4 μg/mL to 128 μg/mL. Among the children of Nepal, those of age less than 1 year were at higher risk of wound infections by bacteria.Staphylococcus aureusfollowed byPseudomonas aeruginosawere the most common bacteria causing wound infections in children.

scholarly journals Synthesis of Fluoroquinolones Derivatives as Antimicrobial Agents

2019 ◽  
Vol 35 (4) ◽  
pp. 1248-1253
Author(s):  
Lubna Swellmeen ◽  
Amal Uzrail ◽  
Rand Shahin ◽  
Yusuf AL-Hiari
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Carboxylic Acid ◽  
Inhibitory Activity ◽  
Antimicrobial Agents ◽  
Gram Negative Bacteria ◽  
Biological Test ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Derivatives Of

Fluoroquinolones are well known to have an anti-infective action. In the present study we described the synthesis of novel florouquinolones derivative as antimicrobial agent. The biological test highlighted a good inhibitory activity for the 7-Chloro-1-Alkyl-6-fluoro-8-nitro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid derived synthons especially against pathogenic Gram-negative bacteria (Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus and Streptococcus agalactiae). The binding interactions were monitored and could explain the good inhibitory activity of the synthesized derivatives of florouquinolones.

scholarly journals Categorization of Bacterial Pathogens Present in Infected Wounds and their Antibiotic Resistance Profile Recovered from Patients Attending Rizgary Hospital-Erbil

2021 ◽  
Vol 9 (2) ◽  
pp. 64-70
Author(s):  
Ahmed A. Al-Naqshbandi ◽  
Hedy A. Hassan ◽  
Mahmoud A. Chawsheen ◽  
Haval H. Abdul Qader
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Resistant Bacteria ◽  
Antibiotic Prescribing ◽  
Gram Negative Bacteria ◽  
Wound Infections ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Wound infection with antibiotic-resistant bacteria can extend a patients’ debility and increase the expense of treatment in the long term; therefore, careful management of patients with wound infections is necessary to avoid complications. The usage of antimicrobial agent is a major factor in resistance development. This study aims to understand the causes of wound infections, as well as the criteria for diagnosing them for more sensible antibiotic prescribing. Samples from 269 wound patients were collected, and cultured for bacterial growth. Gram stain technique, bacterial identification via VITEK 2 compact system were investigated in this study. Gram negative bacteria accounted for 59.15% of the total isolates, while pathogenic gram positive bacteria accounted for 40.85% of total isolates. Escherichia coli and Pseudomonas aeruginosa are the dominant pathogenic gram negative bacteria in wounds, while Staphylococcus aureus, and Staphylococcus epidermidis are the dominant pathogenic gram positive bacteria. Pseudomonas aeruginosa showed 100% resistance to the majority of antibiotic tested, including Ampicillin, Amoxicillin/Clavulanic Acid, Aztreona, Ceftriaxone, and others. Staphylococcus aureus and Staphylococcus epidermidis are 100% resistant to Ampicillin, Ceftriaxone, and Cefotaxime. For more efficient antibiotic prescriptions, the causative microorganisms, and their current susceptibility patterns need to be mandated for testing before prescribing any antibiotics to patients. Prescriptions are frequently based solely on general information about the antibiotic's function, rather than on individual response variation to the pathogen and the antibiotic. Particularly when the common pathogens in this study show multidrug resistance in wounds.

scholarly journals Screening of some plant materials used in South-West Algerian traditional medicine for their antibacterial activity

2017 ◽  
Vol 9 (4) ◽  
pp. 619
Author(s):  
Fatehi Naima ◽  
Houcine BENMEHDI ◽  
Allali Hocine ◽  
Oulednecir Nawal
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Traditional Medicine ◽  
Diffusion Method ◽  
Plant Materials ◽  
Gram Negative ◽  
Maximum Inhibition ◽  
Reference Strains

<p>The initial introduction of new medicinal agents into the health care system sometimes, requires information beyond that is recorded in libraries relying instead, on reports available through traditions and healers within a society. This paper explored the antibacterial activity of aqueous and hydromethanolic extracts of nine folkloric medicinal plant from Bechar region (southwest Algeria) namely: <em>A. nardus, A. schoenanthus, G. vulgaris, </em>two species of<em> H. scoparia green &amp; red, P. laevigata, R. tripartita, T. gallica</em> and <em>T. nudatum</em>, frequently used in the local traditional medicine. The antibacterial activity of different extracts were evaluated by using disc diffusion method agar and antibiotics susceptibility of ten selected microorganisms: seven reference strains, <em>Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus</em>,<em> Escherichia coli</em>,<em> Klebsiella pneumoniae, Pseudomonas aeruginosa</em>, <em>Salmonella typhi</em>, and three clinically isolated strains, <em>Escherichia coli</em> (Urinary Tract Infection), <em>Escherichia coli</em> (Vaginal Infection) and <em>Staphylococcus aureus </em>(Skin Infection).</p><p>The maximum antibacterial activity was recorded against the gram negative reference strains <em>Pseudomonas aeruginosa</em>and<em> Escherichia coli</em> with a maximum inhibition diameter of 15.6 ± 0.5 and 15.0 ± 1.4 mm respectively displayed by the aqueous extract of <em>T. gallica, </em>followed by the activity detected by the hydromethanolic extract of <em>R. tripartita</em> against the gram negative reference strain <em>Pseudomonas aeruginosa </em>(14.6±1.2 mm) and the aqueous and hydromethanolic extracts of <em>R. tripartita</em> against the gram negative reference strains <em>Pseudomonas aeruginosa</em> and<em> Escherichia coli</em> with a maximum inhibition diameter of 14.3 ± 2.0 and 14.3±0.5 mm, respectively.</p><p>According to the present study, <em>H. scoparia red, P. laevigata, R. tripartita, and T. gallica</em>can be served as broad spectrum antibiotic and used as a potent source of natural antibacterial agents by replacing commercially available synthetic drug that may have a large number of side effects.</p>

SYNTHESIS OF PIPERINE - PIPERAZINE ANALOGUES AND THEIR ANTIBACTERIAL ACTIVITY

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 30-35
Author(s):  
Naveen K. Kottakki ◽  
◽  
Amperayani K. Rao ◽  
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Aromatic Nitro ◽  
Heterocyclic Moiety ◽  
Agar Well Diffusion Method

In the current study, a series of piperine – piperazine analogues (5a to 5f) were designed and synthesized. The piperine was isolated from pepper and used for the conjugation with heterocyclic moiety for better biological activity. The piperazine heterocyclic was chosen for conjugation with piperine. The newly synthesized structures were determined by IR, 1H NMR and 13C NMR spectral data. The compounds were examined for their anti‐microbial activity against gram-positive (Bacillus subtilis) and gram-negative (Vibrio cholerae) bacteria using the agar well diffusion method. The newly synthesized compounds exhibited capable activities against V. cholerae and B. subtilis and it showed minimum inhibitory concentration. Among all the synthesized compounds, 5f has the highest activity (26 mm) against gram-positive bacteria and (29mm) against gram-negative bacteria. The remaining compounds showed appreciable antibacterial activity. The enhanced activity of the synthesized compounds may be due to the presence of conjugated amide linkage with the natural product piperine and piperazine heterocyclic molecule. The substituents present on the aromatic (nitro-substituted) ring also influenced the activity of the compound.

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