scholarly journals Extracting Conditions Optimization and Bioactivity of Polysaccharides from the Pods of Haricot Vert

2020 ◽  
Vol 15 (2) ◽  
pp. 45-53
Author(s):  
Nguyen Thi Thu Thuy ◽  
Do Hoang Giang ◽  
Pham Khac Linh ◽  
Nguyen Tien Dat

Polysaccharides from the pods of haricot vert (Phaseolus vulgaris L.) were extracted using a simple heating method, by varying extracting temperature, heating time, solid-to-liquid ratio, and solvent compositions. The obtained results were processed using statistical analysis that helped to identify the optimal conditions for the polysaccharides’ extraction process. This study represents a promising production method of bioactive polysaccharides extract in the food and pharmaceutical industry.

Author(s):  
Hatungimana Françoise ◽  
Hongjun Wang

Various classical Chinese medicines have shown their efficiency in curing various infectious diseases. Among them, Dahuang Qinyu San (DQS) found in the Chinese Veterinary Pharmacopoeia is composed of three kinds of Chinese herbs: rhubarb, Scutellaria baicalensis, and Outtuynia cordata. Due to its urgent need in human health and its effectiveness, a semi-bionic extract of Dahuang Qinyu San (SEDQS) was studied to evaluate its optimal extraction conditions and investigate its antibacterial activity against Salmonella goose. The U5 (53) uniform design method was used to investigate the effects of three independent variables, including pH value (X1), solid-to-liquid ratio (X2), and extraction time (X3), on the composite score (Y) of the extract rate and the MIC, using the semi-bionic extraction process. The broth microdilution method was also used to determine the minimum inhibitory concentration (MIC) against Salmonella goose. After 30 minutes of extraction, the optimal conditions for SEDQS were found to be pH 8.3 and a solidto-liquid ratio of 1: 40. Under these optimal conditions, the extraction rate was 43.66 % and the MIC was 9.10 mg/ml, which indicates antibacterial efficacy against Salmonella goose.


2014 ◽  
Vol 955-959 ◽  
pp. 2794-2802
Author(s):  
Ren Lian Hai

First, through implementing a U24(63) uniform design trial with 3 factors at 6 levels on temperature, heating time and water addition ratio as the main influencing factors of hydrothermal process and analyzing the physicochemical characteristics, the process parameters were optimized in this research. Then, the impacts of the hydrothermal process on the performance of sterilization, de-oiling and dewaterability were studied. It showed that the highest nutrient value of the product appears on condition that the temperature, heating time and water addition ratio were 120 °C, 80 minutes and 30% respectively. Additionally, hydrothermal treatment can kill bacteria thoroughly and affect the dewaterablity of the garbage markedly. Furthermore, hydrothermal process improved the de-oil performance of the garbage, and the optimal conditions for de-oil was the temperature 160 °C and heating time 80 minutes.


Author(s):  
David R. P. Montes ◽  
Rubens A. Oliveira ◽  
Luan B. Giovanelli ◽  
Reginaldo M. de Oliveira ◽  
Júlio C. M. Baptestini ◽  
...  

ABSTRACT Due to the socioeconomic importance of common bean in Brazil, studies that allow establishing favorable conditions for not compromising the crop with white mold, in view of the enormous damages caused by this fungus, become vital to Brazilian agriculture. Therefore, the aim of this study was to evaluate the incidence and severity of white mold in common bean (Phaseolus vulgaris L.), cultivar ‘Madrepérola’, subjected to different irrigation intervals, planting densities and fungicide application. The research was conducted in Viçosa-MG, Brazil, in the years 2011 and 2012. The statistical analysis used the split-split-plot scheme. The fungicide applications (with or without fungicide) were allocated to plots, the irrigation intervals (3, 6, 9 and 12 days) to sub-plots and planting densities (6, 9, 12 and 15 plants per linear meter) to sub-subplots, in a randomized block design with three replicates. The variation of the irrigation intervals did not contribute significantly to the control of white mold. It is recommended to use lower planting densities in order to minimize the damages caused by the disease. The use of the fungicide was the main method of white mold control in two consecutive years.


2013 ◽  
Vol 800 ◽  
pp. 13-17
Author(s):  
Jin Lei Bao ◽  
Xiao Yuan Fan ◽  
Wei Ping Jin ◽  
Bi Bo Liu

Most of photocatalytic reaction taken place in a slurry type reactor, which is of poor light utilization and difficulty to recycling of photocatalyst. To overcome these drawbacks, a floating photocatalyst (TiO2/EP) was prepred by hrothermal method, in which EP(Expanded Perlite) was used as an substrate. Photocatalysts activity was evaluated under UV light using Rhodamine B as the pollutant model. The effect of heating temperature, heating time and dosage of EP on activity of TiO2/EP were Discussed. The results showed 2h and 200°C were the optimal conditions and the dosage of EP has no significant effect on activity of TiO2/EP. The results of XRD and SEM showed TiO2 is anatase form, well crystallized and dispersed; its particle size is about 100~150nm. This floating photocatalyst can remove about 97.4% rhodamine B(10mg/L) during 90min under UV light. It has broad prospects for engineering applications.


Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1927
Author(s):  
Jan Tříska ◽  
Josef Balík ◽  
Milan Houška ◽  
Pavla Novotná ◽  
Martin Magner ◽  
...  

Broccoli sprouts contain 10–100 times higher levels of sulforaphane than mature plants, something that has been well known since 1997. Sulforaphane has a whole range of unique biological properties, and it is especially an inducer of phase 2 detoxication enzymes. Therefore, its use has been intensively studied in the field of health and nutrition. The formation of sulforaphane is controlled by the epithiospecifier protein, a myrosinase co-factor, which is temperature-specific. This paper studies the influence of temperature, heating time, the addition of myrosinase in the form of Raphanus sativus sprouts in constant ratio to broccoli sprouts, and other technological steps on the final sulforaphane content in broccoli sprout homogenates. These technological steps are very important for preserving sulforaphane in broccoli sprouts, but there are some limitations concerning the amount of sulforaphane. We focused, therefore, on the extraction process, using suitable β-cyclodextrin, hexane and ethanol, with the goal of increasing the amount of sulforaphane in the final extract, thus stabilizing it and reducing the required amount sulforaphane needed, e.g., as a dietary supplement.


Author(s):  
Cao Đăng Nguyên ◽  
Nguyễn Thị Cẩm Hạnh

Đã điều tra lectin của 6 giống đậu cô ve thấy rằng cả 6 giống đều có hoạt tính lectin trong đó giống đậu cove hạt trắng dạng bụi (white bean core bush type white seeds) có hoạt tính lectin mạnh nhất, đặc biệt đối với hồng cầu trâu, bò, lợn. Lectin của 6 giống này đều không có biểu hiện đặc hiệu nhóm máu. Lectin đậu cove hạt trắng dạng bụi hoạt động tốt nhất ở nhiệt độ 300C – 400C, pH 6,8 – 7,6. Các đường α-D-glucose, α-D-galactose, D-mannose, D-fructose, D-saccharide,  D-lactose, D-arabinose và D-manitose ở nồng độ 0,05 – 0,1 M có tác dụng kìm hãm hoạt tính của lectin đậu cove hạt trắng dạng bụi. Lectin này cũng bị kìm hãm bởi protein của một số huyết thanh người và động vật (trâu, bò, lợn). Đã tinh sạch lectin đậu cove hạt trắng dạng bụi có độ tinh sạch gấp khoảng 52 lần so với dịch thô ban đầu. Trên gel polyacrylamide thấy xuất hiện 5 band có khối lượng phân tử trong khoảng 30 – 97 kDa.


2006 ◽  
Author(s):  
◽  
Marta Zulema Galván

El poroto común (Phaseolus vulgaris L.) es una leguminosa originaria de América y representa un componente importante en la dieta de la población latinoamericana por su alto contenido en proteínas y carbohidratos. El poroto cultivado se originó de los porotos silvestres, que son plantas anuales, herbáceas y trepadoras, que se distribuyen desde el norte de México hasta el noroeste de la Argentina. Estudios basados en caracteres morfológicos, bioquímicos y moleculares revelaron que tanto dentro de las variedades cultivadas como de las silvestres existen dos acervos génicos principales, uno Andino y el otro Mesoamericano. Las provincias del Noroeste Argentino (NOA) albergan un gran número de porotos silvestres y de variedades locales tradicionales (primitivas o “landraces”) mantenidas durante años en un sistema de cultivo tradicional. Sin embargo muchas de estas poblaciones están en peligro de extinción debido a las presiones de explotación forestal y pastoreo existentes en la zona, por lo que resulta de fundamental importancia su recolección y estudio. En esta tesis se analizó la variabilidad genética de un grupo de poblaciones silvestres y primitivas de poroto común del NOA, recolectadas en diferentes sitios de las provincias fitogeográficas de las Yungas, Prepuna y del Chaco, entre los 1300 y 2900 msnm, utilizando marcadores bioquímicos (proteínas de reserva de las semillas: faseolinas) y moleculares (RAPD e ISSR).


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