scholarly journals Semi-bionic Extraction Process and Antibacterial Activity against Salmonella goose of Compound Chinese Medicine Dahuang Qinyu San

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Hatungimana Françoise ◽  
Hongjun Wang
Keyword(s):  
Antibacterial Activity ◽  
Ph Value ◽  
Design Method ◽  
Uniform Design ◽  
Extraction Process ◽  
Chinese Herbs ◽  
Optimal Conditions ◽  
Liquid Ratio ◽  
Microdilution Method ◽  
Broth Microdilution Method

Various classical Chinese medicines have shown their efficiency in curing various infectious diseases. Among them, Dahuang Qinyu San (DQS) found in the Chinese Veterinary Pharmacopoeia is composed of three kinds of Chinese herbs: rhubarb, Scutellaria baicalensis, and Outtuynia cordata. Due to its urgent need in human health and its effectiveness, a semi-bionic extract of Dahuang Qinyu San (SEDQS) was studied to evaluate its optimal extraction conditions and investigate its antibacterial activity against Salmonella goose. The U5 (53) uniform design method was used to investigate the effects of three independent variables, including pH value (X1), solid-to-liquid ratio (X2), and extraction time (X3), on the composite score (Y) of the extract rate and the MIC, using the semi-bionic extraction process. The broth microdilution method was also used to determine the minimum inhibitory concentration (MIC) against Salmonella goose. After 30 minutes of extraction, the optimal conditions for SEDQS were found to be pH 8.3 and a solidto-liquid ratio of 1: 40. Under these optimal conditions, the extraction rate was 43.66 % and the MIC was 9.10 mg/ml, which indicates antibacterial efficacy against Salmonella goose.

Synthesis and Antibacterial Activities of Amidine Substituted Monocyclic β-Lactams

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijuan Zhai ◽  
Lili He ◽  
Yuanbai Liu ◽  
Ko Ko Myo ◽  
Zafar Iqbal ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Lead Target ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Clinical Interest

Background: Mononcyclic β-lactams are regarded as the most resistant class of β-lactams against a series of β-lactamases though possess limited antibacterial activity. Aztreonam being the first clinically approved monobactam needs broad-spectrum efficacy through structural modification. Objective: We strive to synthesize a number of monocyclic β-lactams by varying the substituents at N1, C3 and C4 positions of azetidinone ring and study the antimicrobial effect on variable bacterial strains. Methods: Seven new monobactam derivatives 23a-g, containing substituted-amidine moieties linked to the azetidinone ring via thiazole linker, were synthesized through multistep synthesis. The final compounds were investigated for their in vitro antibacterial activities using broth microdilution method, against ten bacterial strains of clinical interest. The minimum inhibitory concentrations (MICs) of newly synthesized derivatives were compared with aztreonam, ceftazidime and meropenem, existing clinical antibiotics. Results: All compounds 23a-g showed higher antibacterial activities (MIC 0.25 µg/mL to 64 µg/mL) against tested strains as compared to aztreonam (MIC 16 µg/mL to >64 µg/mL) and ceftazidime (MIC >64 µg/mL). However all compounds, except 23d, exhibited lower antibacterial activity against all tested bacterial strains as compared to meropenem. Conclusion: Compound 23d showed comparable or improved antibacterial activity (MIC 0.25 µg/mL to 2 µg/mL) to meropenem (MIC 1 µg/mL to 2 µg/mL) in case of seven bacterial species. Therefore, compound 23d may be valuable lead target for further investigations against multi-drug resistant Gram-negative bacteria.

scholarly journals Antibacterial Triterpenoids from the Bark of Sonneratia alba (Lythraceae)

2015 ◽  
Vol 10 (2) ◽  
pp. 1934578X1501000
Author(s):  
Harizon ◽  
Betry Pujiastuti ◽  
Dikdik Kurnia ◽  
Dadan Sumiarsa ◽  
Yoshihito Shiono ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Mass Spectrometric ◽  
Broth Microdilution ◽  
Mass Spectrometric Data ◽  
Spectrometric Data ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Sonneratia Alba ◽  
Broth Microdilution Method

The new lupane-type triterpenoid, 3β-hydroxy-lup-9(11), 12-diene, 28-oic acid (1), along with two known lupane-type triterpenoids, lupeol (2) and lupan-3p-ol (3), were isolated from the bark of Sonnetaria alba. The structure of the new compound was elucidated on the basis of spectroscopic and mass spectrometric data analysis. Using the broth microdilution method, all compounds exhibited antibacterial activity against the Gram-positive bacteria Staphylococcus aureus ATCC 6538 and Streptococcus mutans ATCC 25175, with minimum inhibitory concentrations ranging from 15-33 to 35-55 ng/mL, respectively.

scholarly journals Antibacterial Activity of the Essential Oil of Heracleum Sibiricum

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Dragoljub L. Miladinović ◽  
Budimir S. Ilić ◽  
Tatjana M. Mihajilov-Krstev ◽  
Dejan M. Nikolić ◽  
Olga G. Cvetković ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Major Chemical ◽  
Total Oil ◽  
Chemical Class

The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 μg/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 μg/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.

scholarly journals Synthesis andIn VitroEvaluation of New Thiosemicarbazone Derivatives as Potential Antimicrobial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Zafer Asım Kaplancıklı ◽  
Mehlika Dilek Altıntop ◽  
Belgin Sever ◽  
Zerrin Cantürk ◽  
Ahmet Özdemir
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Aromatic Aldehydes ◽  
Antitubercular Activity ◽  
Alamar Blue Assay ◽  
Alamar Blue ◽  
Inhibitory Effects ◽  
Microdilution Method ◽  
Broth Microdilution Method

In an effort to develop potent antimicrobial agents, new thiosemicarbazone derivatives were synthesizedviathe reaction of 4-[4-(trifluoromethyl)phenyl]thiosemicarbazide with aromatic aldehydes. The compounds were evaluated for their inhibitory effects on pathogenic bacteria and yeasts using the CLSI broth microdilution method. Microplate Alamar Blue Assay was also carried out to determine the antimycobacterial activities of the compounds againstMycobacterium tuberculosisH37Rv. Among these derivatives, compounds5and11were more effective againstEnterococcus faecalis(ATCC 29212) than chloramphenicol, whereas compounds1,2, and12and chloramphenicol showed the same level of antibacterial activity againstE. faecalis. Moreover, compound2and chloramphenicol exhibited the same level of antibacterial activity againstStaphylococcus aureus. On the other hand, the most potent anticandidal derivatives were found as compounds2and5. These derivatives and ketoconazole exhibited the same level of antifungal activity againstCandida glabrata. According to the Microplate Alamar Blue Assay, the tested compounds showed weak to moderate antitubercular activity.

scholarly journals Antibacterial Activity of Antibiotic-Impregnated Bone Cement Based Coatings Against Microorganisms with Different Antibiotic Resistance Levels

2018 ◽  
Vol 24 (4) ◽  
pp. 105-110
Author(s):  
D. V. Tapalski ◽  
P. A. Volotovski ◽  
A. I. Kozlova ◽  
A. Sitnik
Keyword(s):  
Antibacterial Activity ◽  
Bone Cement ◽  
Antibiotic Activity ◽  
Broth Microdilution ◽  
Antibiotic Resistant ◽  
Microdilution Method ◽  
Bactericidal Properties ◽  
Broth Microdilution Method ◽  
Marked Activity ◽  
Agar Method

Purpose — to evaluate the presence and duration of antibiotic activity of antibiotic-impregnated bone cement based coatings samples against antibiotic-sensitive and antibiotic-resistant microorganisms.Material and Methods. Bone cement based coatings impregnated with antibiotics (gentamycin, vancomycin, colistin, meropenem, fosfomycin) are formed on titanium (Ti) plates. A plate rinse was carried out; antibiotic concentrations in the rinsed solutions were estimated by a serial broth microdilution method. Antibacterial activity of the control and rinsed samples against the antibiotic-sensitive and multiple-antibiotic-resistant Staphylococcus aureus and Pseudomonas aeruginosa strains was estimated by a bilayer agar method.Results. The meropenem and fosfomycin concentrations in the rinsed solutions obtained at a one-fold (16 μg/ml for both antibiotics) and two-fold treatment (2 μg/ml for meropenem and 8 μg/ml for fosfomycin) were sufficient to suppress the growth of the control strains. One-fold rinse of samples with colistin eliminated their antibacterial activity completely. The marked activity of the samples with meropenem and fosfomycin persisted against the antibiotic-sensitive P. aeruginosa ATCC 27853 strain after 2 rinse cycles; single-rinsed samples with fosfomycin also maintained the activity against the extensively antibioticresistant P. aeruginosa BP-150 strain. Vancomycin-containing samples possessed the sufficient antibacterial activity against both methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) S. aureus strains; two-fold rinse of the samples eliminated their bactericidal properties.Conclusion. Bone cement based coatings impregnated with fosfomycin and meropenem possess the most marked and long-lasting antibacterial activity, manifested mainly against the antibiotic-sensitive strains. 

scholarly journals Identification of Substances Produced by Cercospora brachiata in Absence of Light and Evaluation of Antibacterial Activity

2021 ◽  
Vol 7 (9) ◽  
pp. 680
Author(s):  
John K. R. P. Felisbino ◽  
Bruno S. Vieira ◽  
Alberto de Oliveira ◽  
Neiliane A. da Silva ◽  
Carlos H. G. Martins ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Antibacterial Activity ◽  
Ethyl Acetate Extract ◽  
Phytopathogenic Fungus ◽  
Broth Microdilution ◽  
Actinomyces Naeslundii ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Almost All

Cercospora brachiata is a phytopathogenic fungus. To know more about the metabolites produced by this fungus, the objective of this work was to identify, isolate and characterize substances present in extracts of the growth broth and mycelium, using gas chromatography with mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). It was also objective to evaluate the antibacterial activity of the extracts. Among the compounds identified, fatty acids, esters, and steroids can be highlighted. The main compounds identified are 9-hexadecenoic, hexadecenoic, oleic, octadecanoic, lauric, myristic, palmitic, doceno-13-enoic, stearic, linoleic, and nonadecanoic acids present in almost all extracts. For the antibacterial activity, the broth microdilution method was used. The ethyl acetate extract of the mycelium presented inhibitory concentrations (MICs) against the bacterium Actinomyces naeslundii (100 μg mL−1) and Streptococcus sanguinis (200 μg mL−1). Finally, two steroids were isolated and identified in the hexane extract of mycelium: ergosta-6,22-dien-3β,5α,8α-triol and brassicasterol.

scholarly journals In vitro Antibacterial Activity of Libanotis montana Essential Oil in Combination with Conventional Antibiotics

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Dragoljub L. Miladinović ◽  
Budimir S. Ilić ◽  
Tatjana M. Mihajilov-Krstev ◽  
Jovana L. Jović ◽  
Marija S. Marković
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Hierarchical Cluster ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method

The chemical composition and antibacterial activity were examined of Libanotis montana Crantz subsp. leiocarpa (Heuff.) Soó. (Apiaceae) essential oil. Gas chromatography and gas chromatography/mass spectrometry were used to analyze the chemical composition of the oil. The antibacterial activity was investigated by the broth microdilution method against thirteen bacterial strains. The interactions of the essential oil and three standard antibiotics: tetracycline, streptomycin and chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods: principal components analysis and hierarchical cluster analysis. Sesquiterpene hydrocarbons were the most abundant compound class in the oil (67.2%), with β-elemene (40.4%) as the major compound. The essential oil exhibited slight antibacterial activity against the tested bacterial strains in vitro, but the combinations L. montana oil-chloramphenicol and L. montana oil-tetracycline exhibited mostly either synergistic or additive interactions. These combinations reduced the minimum effective dose of the antibiotics and, consequently, minimized their adverse side effects. In contrast, the association of L. montana essential oil and streptomycin was characterized by strong antagonistic interactions against Escherichia coli ATCC 25922. In the PCA and HCA analyses, streptomycin stood out and formed a separate group.

scholarly journals Antibacterial evaluation of Styrax pohlii and isolated compounds

2013 ◽  
Vol 49 (4) ◽  
pp. 653-658 ◽  
Author(s):  
Camila Spereta Bertanha ◽  
Susane Hellen Utrera ◽  
Valéria Maria Melleiro Gimenez ◽  
Milton Groppo ◽  
Márcio Luis Andrade e Silva ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Preliminary Evaluation ◽  
Microdilution Method ◽  
Aerial Parts ◽  
Broth Microdilution Method ◽  
Weak Antibacterial Activity ◽  
Mic Value

The antibacterial activity of the compounds egonol (1) and homoegonol (2), of the crude ethanolic extract of Styrax pohlii (Styracaceae) aerial parts (EE), and of its n-hexane (HF), EtOAc (EF), n-BuOH (BF), and hydromethanolic (HMF) fractions was evaluated against the following microorganisms: Streptococcus pneumoniae (ATCC 6305), S. pyogenes (ATCC 19615), Haemophilus influenzae (ATCC 10211), Pseudomonas aeruginosa (ATCC 27853), and Klebsiella pneumoniae (ATCC 10031). The broth microdilution method was used for determination of the minimum inhibitory concentration (MIC) during preliminary evaluation of antibacterial activity. The EE yielded MIC values of 400 µg/mL for S. pneumoniae and P. aeruginosa and 300 µg/mL for H. influenzae. The HF and EF fractions exhibited enhanced antibacterial activity, with MIC values of 200 µg/mL against S. pneumoniae, but only EF displayed activity against H. influenzae (MIC 200 µg/mL). The best MIC value with compounds 1 and 2 (400 µg/mL) was obtained for (1) against S. pneumoniae and P. aeruginosa. Therefore, 1 exhibited weak antibacterial activity against these standard strains.

scholarly journals The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-ResistantStaphylococcus aureus

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Young-Seob Lee ◽  
Dae-Young Lee ◽  
Yeon Bok Kim ◽  
Sang-Won Lee ◽  
Seon-Woo Cha ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Low Dose ◽  
Cytoplasmic Membrane ◽  
Antitumor Effects ◽  
Microdilution Method ◽  
Transmission Electron ◽  
Broth Microdilution Method ◽  
Atpase Inhibitors ◽  
Triton X ◽  
Time Kill

Shikonin (SKN), a highly liposoluble naphthoquinone pigment isolated from the roots ofLithospermum erythrorhizon, is known to exert antibacterial, wound-healing, anti-inflammatory, antithrombotic, and antitumor effects. The aim of this study was to examine SKN antibacterial activity against methicillin-resistantStaphylococcus aureus(MRSA). The SKN was analyzed in combination with membrane-permeabilizing agents Tris and Triton X-100, ATPase inhibitors sodium azide andN,N′-dicyclohexylcarbodiimide, andS. aureus-derived peptidoglycan; the effects on MRSA viability were evaluated by the broth microdilution method, time-kill test, and transmission electron microscopy. Addition of membrane-permeabilizing agents or ATPase inhibitors together with a low dose of SKN potentiated SKN anti-MRSA activity, as evidenced by the reduction of MRSA cell density by 75% compared to that observed when SKN was used alone; in contrast, addition of peptidoglycan blocked the antibacterial activity of SKN. The results indicate that the anti-MRSA effect of SKN is associated with its affinity to peptidoglycan, the permeability of the cytoplasmic membrane, and the activity of ATP-binding cassette (ABC) transporters. This study revealed the potential of SKN as an effective natural antibiotic and of its possible use to substantially reduce the use of existing antibiotic may also be important for understanding the mechanism underlying the antibacterial activity of natural compounds.

scholarly journals A New Kind of Quinonic-Antibiotic Useful Against Multidrug-Resistant S. aureus and E. faecium Infections

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1776 ◽  
Author(s):  
Javier Campanini-Salinas ◽  
Juan Andrades-Lagos ◽  
Gerardo Gonzalez Rocha ◽  
Duane Choquesillo-Lazarte ◽  
Soledad Bollo Dragnic ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
New Class ◽  
Microdilution Method ◽  
Mammalian Cell Lines ◽  
Broth Microdilution Method ◽  
In Vitro Antibacterial Activity ◽  
In Vitro Data

A rapid emergence of resistant bacteria is occurring worldwide, endangering the efficacy of antibiotics and reducing the therapeutic arsenal available for treatment of infectious diseases. In the present study, we developed a new class of compounds with antibacterial activity obtained by a simple, two step synthesis and screened the products for in vitro antibacterial activity against ATCC® strains using the broth microdilution method. The compounds exhibited minimum inhibitory concentrations (MIC) of 1–32 μg/mL against Gram-positive ATCC® strains. The structure–activity relationship indicated that the thiophenol ring is essential for antibacterial activity and the substituents on the thiophenol ring module, for antibacterial activity. The most promising compounds detected by screening were tested against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VREF) clinical isolates. We found remarkable activity against VREF for compounds 7 and 16, were the MIC50/90 were 2/4 µg/mL and 4/4 µg/mL, respectively, while for vancomycin the MIC50/90 was 256/512 µg/mL. Neither compound affected cell viability in any of the mammalian cell lines at any of the concentrations tested. These in vitro data show that compounds 7 and 16 have an interesting potential to be developed as new antibacterial drugs against infections caused by VREF.

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