A Comparison of Multidrug Resistance Rates of Pseudomonas Aeruginosa Strains in Burn Patients in Iran in 2006 and 2015

Two hundred swab samples were collected from burn patients and burn units for the period, beginningfrom August 2012 to the end of April 2013 from a number of hospitals in Baghdad governorateincluding: Al-Kindi General Teaching Hospital, Al-Yarmuk General Teaching Hospital and Al-Imam AliHospital. The collected samples were cultured on different media and tested biochemically in order to findout the profile of bacteria that colonize burn patients wounds and environment of burn units. The resultsof bacterial culturing revealed that out of 200 samples, 105 samples 52.5% were observed to havebacterial growth (positive samples), while negative samples represented 95(47.5%). The profile of thebacteria in the cultured samples revealed: Pseudomonas aeruginosa 40.95% was the most common isolatefollowed by Staphylococcus aureus 20.0%, Klebsiella pneumoniae 17.1%, Escherichia coli 8.5%,Pseudomonas putida 4.76%, Enterobacter aerogenes 3.80%, Acinetobacter baumannii 2.85% andProteus mirabilis 1.90%. Forty three P. aeruginosa isolates were tested for antibiotic susceptibility. Theresults showed most isolates were potentially resistant to different antibiotics as follow, all isolates 100%had resistance to Ceftriaxone, Cefepime, and Chloramphenicol, and showed high resistance toTobramycin 95.3%, Gentamicin 93.0%, Ceftazidime 88.3%, Cefotaxime 86.0%, Piperacillin 83.7% andAmikacin 79.0%, beside illustrating low resistance to Aztreonam 67.4%, Ciprofloxacin 46.5%, andImipenem 13.9% among these antibiotics, Imipenem was the most effective antibiotic because 86.0% ofthe isolates appeared to be high sensitive to it.

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Emergence of Multidrug Resistance in Ubiquitous and Dominant Pseudomonas aeruginosa Serogroup O:11

Journal of Clinical Microbiology ◽

10.1128/jcm.36.4.897-901.1998 ◽

1998 ◽

Vol 36 (4) ◽

pp. 897-901 ◽

Cited By ~ 28

Author(s):

Panayotis T. Tassios ◽

Vassiliki Gennimata ◽

Anthony N. Maniatis ◽

Caroline Fock ◽

Nicholas J. Legakis ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Multidrug Resistance ◽

Gel Electrophoresis ◽

Multidrug Resistant ◽

Pulsed Field Gel Electrophoresis ◽

Pulsed Field ◽

Antibiotic Susceptibilities ◽

Resistance Rates ◽

Greek Hospitals ◽

O 11

The serotypes of 88 nonreplicate nosocomial Pseudomonas aeruginosa isolates from 11 Greek hospitals were studied in relation to their antibiotic susceptibilities. Rates of resistance to β-lactams, aminoglycosides, and quinolones ranged from 31 to 65%, except for those to ceftazidime (15%) and imipenem (21%). Four serotypes were dominant: O:12 (25% of isolates), O:1 (17%), O:11 (16%), and O:6 (10%). Multidrug resistance rates in the major serogroups O:12 (91%) and O:11 (79%) were higher than those in serogroups O:1 (40%) and O:6 (43%). Further typing with respect to pulsed-field gel electrophoresis patterns following XbaI digestion of genomic DNA discriminated the isolates into 74 types. Pulsed-field gel electrophoresis revealed that the ubiquitous O:12 group was genetically homogeneous, since 95% of strains belonged to two clusters of genotypic similarity, while the O:11 strains, present in 8 of the 11 hospitals, were distributed among five such clusters. Therefore, apart from the already reported O:12 multidrug-resistant European clone, an O:11 population, characterized by a serotype known to be dominant in the environment and the hospital in several parts of the world, but previously not associated with multidrug resistance to antibiotics, has progressed to a multidrug-resistant state.

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Faculty Opinions recommendation of Clinical prediction tool to identify patients with Pseudomonas aeruginosa respiratory tract infections at greatest risk for multidrug resistance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1067052.520032 ◽

2007 ◽

Author(s):

Gary Lum

Keyword(s):

Pseudomonas Aeruginosa ◽

Multidrug Resistance ◽

Respiratory Tract ◽

Respiratory Tract Infections ◽

Prediction Tool ◽

Clinical Prediction ◽

Tract Infections ◽

Clinical Prediction Tool

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The frequency of genes encoding exotoxin A and exoenzyme S in Pseudomonas aeruginosa strains isolated from burn patients

Burns ◽

10.1016/j.burns.2016.02.012 ◽

2016 ◽

Vol 42 (5) ◽

pp. 1116-1120 ◽

Cited By ~ 13

Author(s):

Azar Dokht Khosravi ◽

Fatemeh Shafie ◽

Effat Abbasi Montazeri ◽

Soodabeh Rostami

Keyword(s):

Pseudomonas Aeruginosa ◽

Burn Patients ◽

Exotoxin A ◽

Exoenzyme S ◽

Genes Encoding

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Detection of Metalo-Beta-Lactamase Gene in Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Lahore, Pakistan

Pakistan BioMedical Journal ◽

10.52229/pbmj.v3i1.4 ◽

2020 ◽

Vol 3 (1) ◽

Author(s):

Humera Kausar ◽

Shabbir Hussain ◽

Afia Muhammad Akram

Keyword(s):

Pseudomonas Aeruginosa ◽

Multiple Drug Resistance ◽

Diffusion Method ◽

Multiple Drug ◽

Carbapenem Resistant ◽

Routine Laboratory Examination ◽

Polymerase Chain ◽

Biochemical Testing ◽

Resistance Rates ◽

Lactamase Gene

Pseudomonas aeruginosa is a widespread organism, caused severe nosocomial infection in human andassociated with multiple drug resistance (MDR) Objective: The present study was carried out to observecurrent antimicrobial resistant pattern of Pseudomonas aeruginosa in Lahore and to detect the Metallobeta-lactamase (MBL) gene in carbapenem resistant Pseudomonas aeruginosa Methods: By screening360 samples total 123 Pseudomonas aeruginosa was identified by standard microbiology techniques suchas microscopy and biochemical testing. The isolated Pseudomonas aeruginosa was evaluated for drugresistance by disc diffusion method and polymerase chain reaction (PCR) was used to identify thecarbapenem resistance causing gene (bla-VIM and bla-IMP) Results: Following antibiotic resistantpattern was observed, Gentamycin (59.00%), Ceftazidime (58.7%), Ceftriaxone (58.00%), Cefotazime(57.0%) and Ciprofloxacin (55.00%). Resistance rates to carbapenem group of antibiotics is Doripenem(30.5%) Meropenem (31.0%) and Imipenem (28.0%). Out of 123 samples of Pseudomonas aeruginosa, 28isolates were found resistant to carbapenem group of antibiotic which was supposed to be highlysensitive for this bacterium. Molecular based identification of resistance genes showed that bla-IMP genewas present in 32.1% (09) and bla-VIM was found positive in 17.8% (04) samples. Metallo-beta-lactamasesproducing genes (bla-VIM and bla-IMP), among carbapenem resistant Pseudomonas aeruginosa weredetected in 28.1% of samples. If other carbapenem resistant gene were also included this number mightbe higher Conclusions: PCR based test should be included in routine laboratory examination for quickdetection of the resistance causing genes.

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