scholarly journals Highly effective silver oxides: influence of oxygen concentration on antibacterial activity against clinical strains of Staphylococcus aureus

2021 ◽  
Vol 5 ◽  
pp. 54-60
Author(s):  
E.M. Gordina ◽  
◽  
S.A. Bozhkova ◽  
A.A. Erusin ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Silver Oxide ◽  
Clinical Strains ◽  
Ion Plasma ◽  
Manual Methods ◽  
Subsequent Measurement ◽  
Minimum Ratio ◽  
Density Results ◽  
Metallic Target

Aim of study. Comparative analysis of antibacterial activity of silver oxides (Ag0,2O, Ag1 O, Ag2 O) in relation to clinical strains of Staphylococcus aureus obtained from patients with orthopaedic infection. Material and methods. Films of silver oxides were coated on paper surface in vacuum via the method of ion-plasma arc sputtering of the metallic target. Isolation of S.aureus was performed via standard manual methods from January to June 2021, identification was carried out via the MALDI-TOF-MS method. Sensitivity to antibiotics was evaluated according to EUCAST (2021, v.11.0) requirements. Antibacterial activity of the silver oxide samples obtained against 130 clinical strains of S.aureus was analysed in liquid nutrient broth with subsequent measurement of optical density. Results. Over 90% of the clinical strains of S.aureus isolated from patients with orthopaedic infection were sensitive to the silver oxides obtained. The amount of oxygen in the compound exerted almost no influence on the sensitivity to the oxides: even a minimum ratio between silver and oxygen provided a pronounced antibacterial effect. Additionally, the sensitivity to silver oxides did not depend on the MRSA resistance phenotype and the compounds were effective against XDR strains of S.aureus in 96.7% of the cases. Conclusion. All tested samples of silver oxides (Ag0,2O, Ag1 O, Ag2O) were characterised by pronounced antibacterial activity against S. aureus. The data obtained point to prospects in application of silver oxides for prevention and treatment of orthopaedic infection induced by Staphylococcus aureus without regard for the sensitivity phenotype of the pathogen.

scholarly journals Comparative evaluation of antibacterial activity of silver oxides with different oxygen content

2021 ◽  
pp. 23-28
Author(s):  
E.M. Gordina ◽  
◽  
S.A. Bozhkova ◽  
A.A. Erusin ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Oxygen Content ◽  
Optical Density ◽  
Antibacterial Properties ◽  
Nutrient Broth ◽  
Low Oxygen ◽  
Pure Silver ◽  
And Staphylococcus Aureus ◽  
Density Results

Aim of the study. To study the presence of antibacterial properties in silver oxides with different oxygen content in relation to sensitive and methicillinresistant Staphylococcus aureus strains. Material and Methods. Films of silver and its oxides were coated on paper surface in vacuum via the method of ion-plasma arc sputtering of the metallic target. Antibacterial activity of the obtained samples of silver oxides and pure silver against Staphylococcus aureus АТСС 29213 and Staphylococcus aureus ATCC 43300 was studied applying the diff usion method as well as in liquid nutrient broth with consequent measuring of optical density. Results. As a result of antibacterial activity investigation of the samples obtained via the diff usion method and liquid nutrient broth, it was revealed that all samples possessed antibacterial activity in relation to type strains of S. aureus. Th erewith, the effi cacy of the samples with pure silver was signifi cantly lower than that of silver oxides (Ag0,2O, Ag0,4O, Ag1O, Ag1,5O, Ag2O). It was shown that the activity of silver oxides did not depend on the oxygen concentration in them. Thus, statistically signifi cant diff erences in optical density of the nutrient medium with bacteria and the medium with incubated S. aureus АТСС 29213 (MSSA) and AgxO (р<0.001). Analogical results were obtained aft er studying of infl uence exerted by silver on S. aureus АТСС 43300 (MRSA). Conclusion. All silver oxides samples created were characterised by antibacterial activity against S. aureus. The data obtained point at the prospects in application of silver oxide with low oxygen content for prevention and treatment of Staphylococcus aureus strains infection.

scholarly journals Antibacterial Activity of Protocatechuic Acid Ethyl Ester on Staphylococcus aureus Clinical Strains Alone and in Combination with Antistaphylococcal Drugs

Molecules ◽  
2015 ◽  
Vol 20 (8) ◽  
pp. 13536-13549 ◽  
Author(s):  
Maria Miklasińska ◽  
Małgorzata Kępa ◽  
Robert Wojtyczka ◽  
Danuta Idzik ◽  
Anna Zdebik ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Ester ◽  
Protocatechuic Acid ◽  
Acid Ethyl Ester ◽  
Clinical Strains

Antibacterial Activity of Desert Truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa

2017 ◽  
Vol 19 (2) ◽  
pp. 121-125 ◽  
Author(s):  
Domenico Schillaci ◽  
Maria Grazia Cusimano ◽  
Stella Maria Cascioferro ◽  
Vita Di Stefano ◽  
Vincenzo Arizza ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Desert Truffles

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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