New sulfonylhydrazones containing methane sulfonic acid hydrazıde havıng human anticarbonic anhydrase and antimicrobıal activıty: synthesis, spectroscopic characterization, electrochemical properties, and biological activıties

Bacterial infections, especially those caused by strains resistant to commonly used antibiotics and chemotherapeutics, are still a current threat to public health. Therefore, the search for new molecules with potential antimicrobial activity is an important research goal. In this article, we present the synthesis and evaluation of the in vitro antimicrobial activity of a series of 15 new derivatives of 4-methyl-1,2,3-thiadiazole-5-carboxylic acid. The potential antimicrobial effect of the new compounds was observed mainly against Gram-positive bacteria. Compound 15, with the 5-nitro-2-furoyl moiety, showed the highest bioactivity: minimum inhibitory concentration (MIC) = 1.95–15.62 µg/mL and minimum bactericidal concentration (MBC)/MIC = 1–4 µg/mL.

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X-ray structure, surface analyses, spectroscopic characterization, antimicrobial activity and DFT studies of the triphenylphosphoranylidene derivative

Molecular Crystals and Liquid Crystals ◽

10.1080/15421406.2020.1848245 ◽

2020 ◽

Vol 711 (1) ◽

pp. 59-77

Author(s):

Ersin İnkaya ◽

Serpil Eryılmaz ◽

Nesuhi Akdemir ◽

Önder İdil

Keyword(s):

Antimicrobial Activity ◽

Spectroscopic Characterization ◽

Dft Studies ◽

X Ray ◽

Structure Surface

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Impact of Altitudinal Variation on the Phytochemical Profile, Anthelmintic and Antimicrobial Activity of Two Pinus Species

Molecules ◽

10.3390/molecules26113170 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3170

Author(s):

Wafaa M. Elkady ◽

Mariam H. Gonaid ◽

Miriam F. Yousif ◽

Mahmoud El-Sayed ◽

Hind A. N. Omar

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Pinus Halepensis ◽

Biological Activities ◽

Anthelmintic Activity ◽

Antimicrobial Activities ◽

Pinus Pinea ◽

Active Components ◽

Pinus Species ◽

Bacteria And Fungi

Active components from natural sources are the current focus in most pharmacological research to provide new therapeutic agents for clinical use. Essential oils from the Pinus species have been traditionally used in medicine. This study aimed to investigate the chemical profile of two Pinus species, Pinus halepensis L. and Pinus pinea Mill, from different altitudes in Libya and study the effect of environmental conditions on the biological activities of essential oils. A clevenger apparatus was used to prepare the essential oils by hydrodistillation. Analyses were done using GC/MS. Anthelmintic and antimicrobial activities were tested against the earthworm Allolobophora caliginosa, gram-positive bacteria, gram-negative bacteria, and fungi. Different chemical profiles were observed among all tested essential oils, and terpenes were the most dominant class. All studied essential oils from the Pinus species exhibited a remarkable anthelmintic activity compared to the standard piperazine citrate drug. Pinus halepensis from both altitudes showed broad-spectrum antimicrobial activity against all tested microorganisms, while Pinus pinea was effective against only Escherichia coli. From these findings, one can conclude that there are variations between studied species. The essential oil compositions are affected by environmental factors, which consequently affect the anthelmintic and antimicrobial activity.

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Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

Indonesian Journal of Pharmacology and Therapy ◽

10.22146/ijpther.1656 ◽

2021 ◽

Vol 2 (2) ◽

Author(s):

Eti Nurwening Sholikhah ◽

Maulina Diah ◽

Mustofa ◽

Masriani ◽

Susi Iravati ◽

...

Keyword(s):

Antimicrobial Activity ◽

Methanolic Extract ◽

Biological Activities ◽

Positive Control ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

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In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2017000400011 ◽

2017 ◽

Vol 37 (4) ◽

pp. 368-378 ◽

Cited By ~ 6

Author(s):

Jusciêne B. Moura ◽

Agueda C. de Vargas ◽

Gisele V. Gouveia ◽

João J. de S. Gouveia ◽

Juracy C. Ramos-Júnior ◽

...

Keyword(s):

Antimicrobial Activity ◽

Biological Activities ◽

Meca Gene ◽

Usnic Acid ◽

Organic Extract ◽

In Vitro Antimicrobial Activity ◽

Low Concentrations ◽

Wide Range ◽

Staphylococcus Spp

ABSTRACT: Cladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.

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Antimicrobial activity of extracellular metabolites from antagonistic bacteria isolated from potato (Solanum phureja) crops

Summa Phytopathologica ◽

10.1590/0100-5405/1953 ◽

2014 ◽

Vol 40 (3) ◽

pp. 212-220 ◽

Cited By ~ 7

Author(s):

Sinar David Granada García ◽

Antoni Rueda Lorza ◽

Carlos Alberto Peláez

Keyword(s):

Antimicrobial Activity ◽

Cyclic Peptide ◽

Biological Activities ◽

Pathogenic Fungi ◽

Antagonistic Activity ◽

Solanum Phureja ◽

Dual Culture ◽

Crude Extracts ◽

Extracellular Metabolites

Microorganisms for biological control are capable of producing active compounds that inhibit the development of phytopathogens, constituting a promising tool toob tain active principles that could replace synthetic pesticides. This study evaluatedtheability of severalpotentialbiocontrol microorganismsto produce active extracellular metabolites. In vitro antagonistic capability of 50 bacterial isolates from rhizospheric soils of "criolla" potato (Solanum phureja) was tested through dual culture in this plant with different plant pathogenic fungi and bacteria. Isolates that showed significantly higher antagonistic activity were fermented in liquid media and crude extracts from the supernatants had their biological activities assessed by optical density techniques. Inhibitory effecton tested pathogens was observed for concentrations between 0.5% and 1% of crude extracts. There was a correlation between the antimicrobial activity of extracts and the use of nutrient-rich media in bacteria fermentation. Using a bioguided method, a peptidic compound, active against Fusarium oxysporum, was obtained from the 7ANT04 strain (Pyrobaculum sp.). Analysis by nuclear magnetic resonance and liquid chromatography coupled to mass detector evidenced an 11-amino acid compound. Bioinformatic software using raw mass data confirmed the presence of a cyclic peptide conformed by 11 mostly non-standard amino acids.

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