Research connecting neighborhoods and health has characterized neighborhood factors in multiple ways. This chapter discusses standard and emerging methods to measure and study neighborhood characteristics. In particular, this chapter provides an overview of neighborhood characteristic assessment methods, including self-report, systematic social observation, geographic information system (GIS) methods, Web-based geospatial methods, real-time geospatial methods, crowd-sourced geospatial methods, and information retrieval methods from online sources such as Instagram and Twitter. This chapter also discusses the strengths and limitations of each neighborhood characteristic assessment method (e.g., ease of administration, validity), and readers are provided with examples of each neighborhood assessment method applied in the epidemiology and population health literature.