Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process

Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a control group, (k2) sperm preparation group without ALA, (k3) group of sperm preparation with the addition of ALA. The motility of spermatozoa was observed with the WHO 1999 method for 4 hours in units of percent. Progressive motility in k3 (47.95 ± 3.617) was higher than in k2 (38.05 ± 3.278) statistically significantly different after 3 hours of observation (p<0.0001). Progressive motility in k3 (78.8 ± 5.841) was higher than k1 (56.55 ± 7.511) from the initial observation (p <0.0001). The progressive motility of k2 (76.05 ± 6.768) was higher than k1 (56.55 ± 7.511) from the start of the observation (0.0001). It can be concluded that the addition of ALA in the sperm preparation process increases and maintains progressive motility that is better than sperm preparation without ALA addition after 3 hours of observation.

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Chemopreventive Effects of Alpha Lipoic Acid on Obesity-Related Cancers

Annals of Nutrition and Metabolism ◽

10.1159/000443994 ◽

2016 ◽

Vol 68 (2) ◽

pp. 137-144 ◽

Cited By ~ 13

Author(s):

Hyun-Seuk Moon

Keyword(s):

Risk Factor ◽

Lipoic Acid ◽

Octanoic Acid ◽

Scattered Data ◽

Alpha Lipoic Acid ◽

Chemopreventive Agent ◽

Anti Cancer ◽

In Vivo Animal Models

Background: It has been generally accepted that being overweight or obese is a risk factor for several types of cancers, including breast, thyroid, colon, pancreatic and liver. In fact, people who are obese have more fat tissues that can produce hormones, such as insulin or estrogen, which may cause cancer cells to grow. Alpha lipoic acid (ALA) is anorganosulfur compound derived from octanoic acid, which is produced in animals normally, and is essential for aerobic metabolism. Summary: Studies in both in vitro cells and in vivo animal models have shown that ALA inhibits the initiation and promotion stages of carcinogenesis, suggesting that ALA has considerable attention as a chemopreventive agent. This brief review collects the scattered data available in the literature concerning ALA and highlights its anti-cancer properties, intermediary metabolism and exploratory implications. Key Messages: Based on scientific evidences so far, ALA might be useful agents in the management or chemoprevention of obesity-related cancers.

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Effect of alpha-lipoic acid on antioxidant gene expression and kidney injury in alloxan-induced diabetic rats

Journal of Nephropathology ◽

10.15171/jnp.2019.06 ◽

2018 ◽

Vol 8 (1) ◽

pp. 6-6 ◽

Cited By ~ 2

Author(s):

Parisa Jamor ◽

Hassan Ahmadvand ◽

Hesam Ashoory ◽

Esmaeel Babaeenezhad

Keyword(s):

Gene Expression ◽

Lipoic Acid ◽

Kidney Injury ◽

Diabetic Rats ◽

Pcr Analysis ◽

Control Group ◽

Diabetic Patients ◽

Alpha Lipoic Acid ◽

The Impact ◽

Gpx Activity

Background: Myeloperoxidase (MPO) is involved in the initiation, progression, and complications of atherosclerosis in diabetic patients. Objectives: In the current study, the impact of alpha-lipoic acid (LA), a natural antioxidant and a cofactor in the enzyme complexes on MPO, catalase (CAT) and glutathione peroxidase (GPx) activity, glutathione (GSH) and malondialdehyde (MDA) level, histopathology of kidney and expression of antioxidant enzymes, superoxide dismutase (SOD), GPx and CAT which are involved in the detoxification of reactive oxygen species (ROS), was evaluated in alloxan-induced diabetic rats. Materials and Methods: In this study, 30 male Rattus norvegicus rats randomly divided into three groups; control (C), non-treated diabetic (NTD), and LA-treated diabetics (LATD) was induced by alloxan monohydrate (100mg/kg; subcutaneous [SC]). Then treatment was performed with alphaLA (100 mg/kg intraperitoneal (i.p) daily to 6 weeks). Blood sample of animals collected to measure levels of MPO, CAT and GPx activity GSH and MDA. Kidney paraffin sections were prepared to estimate histological studies and to measure quantitative gene expression SOD, GPX and CAT in kidney. Results: Induction of diabetes led to a significant increase in MPO and MDA, reduced GSH level and GPx and CAT activities (P < 0.05). However, treatment with alpha-LA led to a significant elevation in GPx, CAT and GSH levels with a reduction in MPO activities and MDA levels (P < 0.05). Furthermore, the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis results showed increased expressions of GPx, CAT and SOD enzyme in the treatment group compared with the diabetic control group. Histopathological lesions such as increased glomerular volume and lymphocyte infiltration were attenuated in the alpha-LA treated group. Conclusions: Our findings indicated that alpha-LA supplementation is effective in preventing complications induced by oxidative stress and atherosclerosis in diabetic rats.

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Effect of alpha lipoic acid on in vitro development of bovine secondary preantral follicles

Theriogenology ◽

10.1016/j.theriogenology.2016.09.013 ◽

2017 ◽

Vol 88 ◽

pp. 124-130 ◽

Cited By ~ 5

Author(s):

Khairy M.A. Zoheir ◽

Gamaleldin I. Harisa ◽

Ahmed A. Allam ◽

Liguo Yang ◽

Xiang Li ◽

...

Keyword(s):

Lipoic Acid ◽

Alpha Lipoic Acid ◽

In Vitro Development ◽

Preantral Follicles ◽

Vitro Development

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Abstract 440: Activation of Hepatic CREBH-Insig-2a Signaling in the Triglyceride-Lowering Mechanism of R-Alpha-Lipoic Acid

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.34.suppl_1.440 ◽

2014 ◽

Vol 34 (suppl_1) ◽

Author(s):

Xuedong Tong ◽

Regis Moreau ◽

Qiaozhu Su

Keyword(s):

Lipoic Acid ◽

De Novo ◽

Therapeutic Potential ◽

Regulatory Element ◽

Ldl Receptor ◽

De Novo Lipogenesis ◽

Alpha Lipoic Acid ◽

Very Low Density Lipoproteins ◽

Expression Of Genes

Activation of the sterol regulatory element-binding proteins (SREBPs), a step regulated by a cluster of ER-resident proteins, Insig-1, Insig-2 and SCAP, is rate limiting in hepatic de novo lipogenesis. We previously reported that feeding R-alpha-lipoic acid (LA) to ZDF (fa/fa) rats improves severe hypertriglyceridemia and lowers abdominal fat mass by inhibiting expression of genes involved in hepatic long-chain fatty acids and triacylglycerol syntheses. In this study, we characterized a novel mechanism of action of LA that explains its triacylglycerol lowering properties. Dietary LA activates liver specific transcription factor cAMP responsive element binding protein H (CREBH), which in turn enhances transcription and translation of Insig-1 and Insig-2. Chromatin immunoprecipitation (ChIP) assay demonstrated interaction between CREBH and the promoter of Insig-2 but not Insig-1. The increased abundance of Insig-1 and Insig-2 proteins contributes to sequester SREBP-1c and SREBP-2 in the ER and prevents their translocation to the Golgi apparatus where they would become activated. As a consequence, mRNA expression of genes involved in fatty acid and cholesterol synthesis, including FASN, ACC, SCD-1, HMGCR and LDL receptor, were significantly decreased in LA-fed animals versus pair-fed controls. Concomitantly, the assembly and secretion of very-low-density lipoproteins (VLDL) by primary hepatocytes were suppressed in the LA-fed ZDF rats as indicated by the decrease in VLDL-associated apolipoprotein B and apolipoprotein E. In vitro, treating a rat McA-RH7777 hepatoma cells with LA (200 micromole) activated CREBH, induced expression of Insig-1 and Insig-2, and hindered the palmitic acid-induced synthesis of triacylglycerol. This study provides new mechanistic insight into the triacylglycerol lowering properties of LA and supports the therapeutic potential of LA against hypertriglyceridemia.

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Alpha lipoic acid reverses the negative effect of LPS on mouse spermatozoa and developmental competence of resultant embryos in vitro

Andrology ◽

10.1111/andr.12596 ◽

2019 ◽

Vol 7 (3) ◽

pp. 350-356 ◽

Cited By ~ 3

Author(s):

A. Makvandi ◽

R. Kowsar ◽

M. Hajian ◽

A. H. Mahdavi ◽

N. Tanhaei Vash ◽

...

Keyword(s):

Lipoic Acid ◽

Developmental Competence ◽

Alpha Lipoic Acid ◽

Negative Effect

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Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Zygote ◽

10.1017/s0967199415000155 ◽

2015 ◽

Vol 24 (2) ◽

pp. 259-265 ◽

Cited By ~ 10

Author(s):

Tao Shen ◽

Zhong-Liang Jiang ◽

Cong-Jun Li ◽

Xiao-Chen Hu ◽

Qing-Wang Li

Keyword(s):

Lipoic Acid ◽

Artificial Insemination ◽

Antioxidant Effect ◽

Control Group ◽

Antioxidant Enzyme Activities ◽

Alpha Lipoic Acid ◽

Glutamic Oxaloacetic Transaminase ◽

Boar Semen ◽

Endogenous Antioxidant ◽

Boar Spermatozoa

SummaryAlpha-lipoic acid (ALA) is known to be a natural antioxidant. The aim of the present study was to evaluate the cryoprotective effect of ALA on the motility of boar spermatozoa and its antioxidant effect on boar spermatozoa during freezing–thawing. Different concentrations (2.0, 4.0, 6.0, 8.0 or 10.0 mg/ml) of ALA were added to the extender used to freeze boar semen, and the effects on the quality and endogenous antioxidant enzyme activities of frozen–thawed spermatozoa were assessed. The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post-thaw (P < 0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxaloacetic transaminase and catalase improved after adding ALA to the extender (P < 0.05). Artificial insemination results showed that pregnancy rate and litter size were significantly higher at 6.0 mg/ml in the ALA group than in the control group (P < 0.05). In conclusion, ALA conferred a cryoprotective capacity to the extender used for boar semen during the process of freezing–thawing, and the optimal concentration of ALA for the frozen extender was 6.0 mg/ml.

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