scholarly journals Effect of High Fat Diet on Weight Loss Through the Expression of Uncouple Protein 1 in Mice Visceral Fat

2021 ◽  
Vol 56 (3) ◽  
pp. 223
Author(s):  
Indira Syahraya ◽  
Hermina Novida ◽  
Lilik Herawati ◽  
Purwo Sri Rejeki
Keyword(s):  
Weight Loss ◽  
Visceral Fat ◽  
High Fat Diet ◽  
Significant Weight Loss ◽  
Uncouple Protein ◽  
High Fat ◽  
Immunohistochemistry Staining ◽  
Key Factor ◽  
Weight Loss Group ◽  
Ucp1 Expression

Obesity has become an epidemic around the world. High fat diet (HFD) have been implemented as one of intervention to battle obesity. Uncouple protein 1 (UCP1) is one of the key factor on energy expenditure. The aim of this experiment is to see the macronutrients composition on weight loss and UCP1 expression in the visceral fat. Fifty male mice, 2-3 months old, 18-30 grams, were put in five different groups. K1 were fed (20% protein, 62.0% carbohydrate, 12% fat), K2 (60% protein, 0% carbohydrate, 30% fat), K3 (45% protein, 0% carbohydrate, 45% fat), K4 (30% protein, 0% carbohydrate, 60% fat), K5 (15% protein, 0% carbohydrate, 75% fat). The experiment was done in four weeks, mice body weight was measured every week. UCP1 expression seen using immunohistochemistry staining was measured at the end of the fourth week. Significant weight loss was achieved by K4 (-9.60±3.81) gram by the end of week four (p<0.05). K4 had the least amount of visceral fat. The result was that K4 achieved a significant visceral fat mass (0.02±0.06) gram compared to K1 (0.53±0.08) gram. Compared to other groups K5 expressed UCP1 more than the others (3.78±3.72) cphfp. HFD fed groups produced significant weight loss, group that had the greatest weight loss is K4. Meanwhile, each group had a variety of UCP1 expression.

scholarly journals Effect of High Fat Diet on Weight Loss Through the Expression of Uncouple Protein 1 in Mice Visceral Fat

2020 ◽  
Vol 56 (3) ◽  
pp. 223
Author(s):  
Indira Syahraya ◽  
Hermina Novida ◽  
Lilik Herawati ◽  
Purwo Sri Rejeki
Keyword(s):  
Weight Loss ◽  
Visceral Fat ◽  
High Fat Diet ◽  
Significant Weight Loss ◽  
Uncouple Protein ◽  
High Fat ◽  
Immunohistochemistry Staining ◽  
Key Factor ◽  
Weight Loss Group ◽  
Ucp1 Expression

Obesity has become an epidemic around the world. High fat diet (HFD) have been implemented as one of intervention to battle obesity. Uncouple protein 1 (UCP1) is one of the key factor on energy expenditure. The aim of this experiment is to see the macronutrients composition on weight loss and UCP1 expression in the visceral fat. Fifty male mice, 2-3 months old, 18-30 grams, were put in five different groups. K1 (20% protein, 62.0% carbohydrate, 12% fat), K2 (60% protein, 0% carbohydrate, 30% fat), K3 (45% protein, 0% carbohydrate, 45% fat), K4 (30% protein, 0% carbohydrate, 60% fat), K5 (15% protein, 0% carbohydrate, 75% fat). The experiment was done in four weeks, mice body weight was measured every week. UCP1 expression seen using immunohistochemistry staining was measured at the end of the fourth week. Significant weight loss was achieved by K4 (-9.60±3.81) gram by the end of week four (p<0.05). K4 had the least amount of visceral fat. The result was that K4 achieved a significant visceral fat mass (0.02±0.06) gram compared to K1 (0.53±0.08) gram. Compared to other groups K5 expressed UCP1 more than the others (3.78±3.72) cphfp. HFD fed groups produced significant weight loss, group that had the greatest weight loss is K4. Meanwhile, each group had a variety of UCP1 expression.

A New Physiologic Mouse Model of One Anastomosis Gastric Bypass

2018 ◽  
Vol 59 (5-6) ◽  
pp. 320-328
Author(s):  
Andreas Kroh ◽  
Roman M. Eickhoff ◽  
Daniel Heise ◽  
Patrick H. Alizai ◽  
Karl P. Rheinwalt ◽  
...  
Keyword(s):  
Weight Loss ◽  
Gastric Bypass ◽  
Mouse Model ◽  
High Fat Diet ◽  
One Anastomosis Gastric Bypass ◽  
Sham Operation ◽  
Significant Weight Loss ◽  
Long Term Effects ◽  
High Fat

Background: One anastomosis gastric bypass (OAGB) is a modern metabolic operation that has been demonstrated to be a rapid, safe, and effective procedure. As for other bariatric operations, the mechanisms and long-term effects of this procedure remain largely unknown and are difficult to address in human studies. Here, we present a new physiologic mouse model for mechanistic and long-term investigations. Methods: Six-week-old C57Bl/6 mice were fed a high-fat diet for 12 weeks and scheduled for OAGB or sham operation. Mice were observed for 2 weeks after the operation, and weight and metabolic condition were monitored. Results: Six mice were used to adapt the surgical technique. Afterwards, another 7 mice were scheduled for OAGB without further complications. The newly established OAGB procedure resulted in significant weight loss and improvement of glucose metabolism 2 weeks after the operation. Conclusions: The operation presented here is an easy-to-learn and physiologic mouse model of OAGB that can be used for further studies in mice.

scholarly journals Replacing a Palatable High-Fat Diet with a Low-Fat Alternative Heightens κ-Opioid Receptor Control over Nucleus Accumbens Dopamine

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2341
Author(s):  
Conner W. Wallace ◽  
Nari S. Beatty ◽  
Sarah A. Hutcherson ◽  
Heather A. Emmons ◽  
Madison C. Loudermilt ◽  
...  
Keyword(s):  
Weight Loss ◽  
Nucleus Accumbens ◽  
High Fat Diet ◽  
Elevated Plus Maze ◽  
High Fat ◽  
Food Cravings ◽  
Fast Scan Cyclic Voltammetry ◽  
Diet Induced Obesity ◽  
Plus Maze ◽  
Weight Loss Interventions

Diet-induced obesity reduces dopaminergic neurotransmission in the nucleus accumbens (NAc), and stressful weight loss interventions could promote cravings for palatable foods high in fat and sugar that stimulate dopamine. Activation of κ-opioid receptors (KORs) reduces synaptic dopamine, but contribution of KORs to lower dopamine tone after dietary changes is unknown. Therefore, the purpose of this study was to determine the function of KORs in C57BL/6 mice that consumed a 60% high-fat diet (HFD) for six weeks followed by replacement of HFD with a control 10% fat diet for one day or one week. HFD replacement induced voluntary caloric restriction and weight loss. However, fast-scan cyclic voltammetry revealed no differences in baseline dopamine parameters, whereas sex effects were revealed during KOR stimulation. NAc core dopamine release was reduced by KOR agonism after one day of HFD replacement in females but after one week of HFD replacement in males. Further, elevated plus-maze testing revealed no diet effects during HFD replacement on overt anxiety. These results suggest that KORs reduce NAc dopamine tone and increase food-related anxiety during dietary weight loss interventions that could subsequently promote palatable food cravings and inhibit weight loss.

scholarly journals Expression of eotaxin in 3T3-L1 adipocytes and the effects of weight loss in high-fat diet induced obese mice

2011 ◽  
Vol 5 (1) ◽  
pp. 11 ◽  
Author(s):  
Hyun-Jung Kim ◽  
Chang-Hyun Kim ◽  
Do-Hyun Lee ◽  
Min-Woo Han ◽  
Mi-Young Kim ◽  
...  
Keyword(s):  
Weight Loss ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat

scholarly journals α-Methyl-L-Tryptophan as a Weight-Loss Agent in Multiple Models of Obesity in Mice

2021 ◽  
Author(s):  
Sathish Sivaprakasam ◽  
Sabarish Ramachandran ◽  
Mohd Omar Faruk Sikder ◽  
Yangzom Doma Bhutia ◽  
Mitchell Wachtel ◽  
...  
Keyword(s):  
Weight Loss ◽  
High Fat Diet ◽  
Amino Acid Profile ◽  
Normal Diet ◽  
The Body ◽  
Multiple Models ◽  
High Fat ◽  
Liver And Kidney ◽  
Obesity In Mice ◽  
Treatment Of Obesity

a-Methyl-L-tryptophan (a-MLT) is currently in use as a tracer in its 11C-labeled form to monitor the health of serotonergic neurons in humans. In the present study, we found this compound to function as an effective weight-loss agent at pharmacological doses in multiple models of obesity in mice. The drug was able to reduce the body weight when given orally in drinking water (1 mg/ml) in three different models of obesity: normal mice on high-fat diet, Slc6a14-null mice on high-fat diet, and ob/ob mice on normal diet. Only the L-enantiomer (a-MLT) was active while the D-enantiomer (a-MDT) had negligible activity. The weight-loss effect was freely reversible, with the weight gain resuming soon after the withdrawal of the drug. All three models of obesity were associated with hyperglycemia, insulin resistance, and hepatic steatosis; a-MLT reversed these features. There was a decrease in food intake in the treatment group. Mice on a high-fat diet showed decreased cholesterol and protein in the serum when treated with a-MLT; there was however no evidence of liver and kidney dysfunction. Plasma amino acid profile indicated a significant decrease in the levels of specific amino acids, including tryptophan; but the levels of arginine were increased. We conclude that a-MLT is an effective, reversible, and orally active drug for the treatment of obesity and metabolic syndrome.

scholarly journals Inhibitor of Differentiation-3 Mediates High Fat Diet-Induced Visceral Fat Expansion

2012 ◽  
Vol 32 (2) ◽  
pp. 317-324 ◽  
Author(s):  
Alexis Cutchins ◽  
Daniel B. Harmon ◽  
Jennifer L. Kirby ◽  
Amanda C. Doran ◽  
Stephanie N. Oldham ◽  
...  
Keyword(s):  
Visceral Fat ◽  
High Fat Diet ◽  
High Fat

Dietary d-Psicose Reduced Visceral Fat Mass in High-Fat Diet-Induced Obese Rats

2012 ◽  
Vol 77 (2) ◽  
pp. H53-H58 ◽  
Author(s):  
Young-Mee Chung ◽  
Joo Hyun Lee ◽  
Deuk Youl Kim ◽  
Se-Hee Hwang ◽  
Young-Ho Hong ◽  
...  
Keyword(s):  
Fat Mass ◽  
Visceral Fat ◽  
High Fat Diet ◽  
High Fat ◽  
Obese Rats

Abstract 686: Intervention with Naringenin Enhances Weight Loss, Potentiates Improvements in Metabolic Dysregulation and Halts Progression of Atherosclerosis Induced by a High-Fat Diet in LDLr-/- Mice.

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Amy C Burke ◽  
Brian G Sutherland ◽  
Julia M Assini ◽  
Murray W Huff
Keyword(s):  
Insulin Resistance ◽  
Weight Loss ◽  
Plasma Glucose ◽  
High Fat Diet ◽  
Lesion Size ◽  
Chow Diet ◽  
High Fat ◽  
Metabolic Dysregulation ◽  
The Metabolic Syndrome ◽  
Progression Of Atherosclerosis

Previous studies demonstrate that the addition of naringenin, a grapefruit flavonoid, to a high-fat diet prevents the development of many disorders of the metabolic syndrome and atherosclerosis in Ldlr-/- mice. Furthermore, in intervention studies, the addition of naringenin to a high-fat, high cholesterol (HFHC) diet reversed pre-established obesity, hyperlipidemia, hepatic steatosis, insulin resistance and improved atherosclerotic lesion pathology, but not lesion size. In the present intervention study, we tested the hypothesis that addition of naringenin to a chow diet would further improve pre-established metabolic dysregulation and attenuate lesion development, compared to chow alone. Ldlr-/- mice were fed a HFHC diet for 12 weeks to induce metabolic dysregulation. Subsequently, mice received one of 3 diets for another 12 weeks: 1) continuation of the HFHC diet, 2) an isoflavone-free chow diet or 3) isoflavone-free chow with 3% naringenin. At 12 weeks, the HFHC diet induced significant weight gain and increased adiposity. Intervention with chow alone reduced the weight gained during induction by 22%, whereas the addition of naringenin to chow induced a weight loss of 71%. Specifically, the reduction in adiposity was 2.75-times greater in naringenin-treated mice, compared to chow alone. The HFHC diet increased VLDL cholesterol 20-fold and LDL cholesterol 5-fold, which were reduced by intervention with both chow (>60%) and chow supplemented with naringenin (>80%). The HFHC diet induced insulin resistance and glucose intolerance. Naringenin improved insulin tolerance (plasma glucose AUC -38%) and glucose tolerance (plasma glucose AUC -58%), which was accompanied by normalization of plasma insulin and glucose. HFHC-induction promoted the development of intermediate atherosclerotic lesions. Continuation of the HFHC diet doubled lesion size. Intervention with chow alone attenuated lesion size progression by 65%. The addition of naringenin to chow slowed lesion progression by 90%, resulting in smaller lesions compared to chow intervention alone (P=0.042). We conclude that intervention with naringenin-supplemented chow enhances weight loss, improves metabolic dysregulation and halts the progression of atherosclerosis.

scholarly journals High-fat diet induced central adiposity (visceral fat) is associated with increased fibrosis and decreased immune cellularity of the mesenteric lymph node in mice

2019 ◽  
Vol 59 (4) ◽  
pp. 1641-1654 ◽  
Author(s):  
Aaron M. Magnuson ◽  
Daniel P. Regan ◽  
Andrea D. Booth ◽  
Josephine K. Fouts ◽  
Claudia M. Solt ◽  
...  
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Visceral Fat ◽  
High Fat Diet ◽  
Central Adiposity ◽  
High Fat ◽  
Mesenteric Lymph
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