FLOCCULANTS OPTIMIZATION IN HARVESTING FRESHWATER MICROALGAE Haematococcus pluvialis

Microalgae-based researches are one of the interesting topics to date due to its wide applications for commercial industries. One of the vital issues that remain in the production of large-scale microalgae is the harvesting process of microalgae culture. There are several techniques that can be used in harvesting microalgae and each algae will be showing a different result. In this research, the microalgae Haematococcus pluvialis were harvested using flocculation-filtration method. Alum in different dosages, range from 0.25-2 g/L, was used as flocculants and the mixing duration was investigated to seek out the best harvesting efficiency. Based on the research, it can be concluded that the optimum flocculation process was alum addition of 1 g/L culture that continuously stirring for 5 minutes long. In which the flocculation efficiency reaches 94.5%.

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Bio-Flocculation Property Analyses of Oleaginous Microalgae Auxenochlorella protothecoides UTEX 2341

Sustainability ◽

10.3390/su13052885 ◽

2021 ◽

Vol 13 (5) ◽

pp. 2885

Author(s):

Jinyu Li ◽

Baozhen Li ◽

Jinshui Yang

Keyword(s):

Large Scale ◽

Municipal Wastewater ◽

Biofuel Production ◽

Microalgae Harvesting ◽

Flocculation Efficiency ◽

Oleaginous Microalgae ◽

Freeze Dried ◽

Flocculation Ability ◽

Powder Samples ◽

Auxenochlorella Protothecoides

The bio-flocculation ability of UTEX 2341 was studied for the purpose of improving microalgae harvesting efficiency to cut the high cost of biofuel production. The algae cells of UTEX 2341 cultured under heterotrophic and municipal wastewater conditions were found to have better self-flocculation ability, with flocculation rates of 92% and 85% at 2 h, respectively. Moreover, the flocculation rates of 16 freeze-dried microalgae powder samples cultured under different stress conditions were 0~72% with an algae powder dosage of 35 mg L−1. The flocculation efficiency of DIM, DCd1, DT28, and L6S was stable under different pH of 3~9 and temperatures of 15~50 °C. For samples of IM, LCd0.6, LMn2, and LZn2, the flocculation efficiency decreased or increased respectively with increased pH or temperatures. Though the flocculation properties of the eight samples showed wide differences, their flocculant compositions were almost the same with unknown components occupying large proportions. More studies needed to be further carried out to reveal the flocculation mechanisms and analyze the flocculation abilities in practical application, which would be conducive to future large-scale application of the bio-flocculation method and also cost reduction.

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Optimization of flocculation efficiency and settling time using chitosan and eggshell as bio-flocculant in Chlorella pyrenoidosa harvesting process

Environmental Technology & Innovation ◽

10.1016/j.eti.2021.101959 ◽

2021 ◽

Vol 24 ◽

pp. 101959

Author(s):

H. Hadiyanto ◽

Marcelinus Christwardana ◽

W. Widayat ◽

Andono Kusuma Jati ◽

Salsabella Indy Laes

Keyword(s):

Chlorella Pyrenoidosa ◽

Settling Time ◽

Flocculation Efficiency ◽

Harvesting Process

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Cultivation of Haematococcus pluvialis for astaxanthin production on angled bench-scale and large-scale biofilm-based photobioreactors

Vietnam Journal of Science Technology and Engineering ◽

10.31276/vjste.61(3).61-70 ◽

2019 ◽

Vol 61 (3) ◽

pp. 61-70 ◽

Cited By ~ 3

Author(s):

Hoang-Dung Tran ◽

Thanh-Tri Do ◽

Tuan-Loc Le ◽

Minh-Ly Tran Nguyen ◽

Cong-Hoat Pham ◽

...

Keyword(s):

Large Scale ◽

Haematococcus Pluvialis ◽

Bench Scale ◽

Astaxanthin Production

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Infection with intracellular parasite Amoeboaphelidium protococcarum induces shifts in associated bacterial communities in microalgae cultures

Journal of Applied Phycology ◽

10.1007/s10811-021-02542-9 ◽

2021 ◽

Vol 33 (5) ◽

pp. 2863-2873

Author(s):

Anna-Lena Höger ◽

Carola Griehl ◽

Matthias Noll

Keyword(s):

Bacterial Communities ◽

Large Scale ◽

Algal Growth ◽

Dry Weight ◽

Illumina Miseq ◽

Culture Conditions ◽

Rrna Gene ◽

Scale Production ◽

Market Demand ◽

Freshwater Microalgae

AbstractIn recent years microalgae products have developed increasing market demand, but sustainable industrial production is still challenged by biological stability of large-scale production plants. Yet the relationships between algal hosts, associated microbiomes, and contaminants in photobioreactors remains widely understudied. The aim of this study was to investigate the temporal development of microbiomes of four freshwater microalgae species Scenedesmus vacuolatus, Desmodesmus quadricauda, Chlorella sorokiniana, and Botryococcus braunii, in presence and absence of the zoosporic parasite Amoeboaphelidium protococcarum. To compare the effects of sterile and nonsterile culture conditions, infection experiments were performed in sterile laboratory (sterile) and simulated industrial conditions (open). Algal growth (dry weight, optical density, and nutrient consumption) was observed for 21 days, and samples of the associated microbiome were collected for bacterial 16S rRNA gene Illumina MiSeq sequencing. Infection patterns of A. protococcarum were algae species-specific, irrespectively of culture conditions. Bacterial community analysis demonstrated distinct and stable bacterial communities for each algae species, which were mostly dominated by α- and γ-Proteobacteria. Upon aphelid parasitosis, bacterial diversity increased, and community compositions diverged algae-specific over time. Moreover, bacterial functional traits shifted to detoxification, degradation, and cellulolysis once algae were infected. This study provides a first insight into the close connection between algae, associated bacterial microbiomes and appearing contaminants in photobioreactor systems.

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A low-cost and scalable process for harvesting microalgae using commercial-grade flocculant

RSC Advances ◽

10.1039/c9ra08072d ◽

2019 ◽

Vol 9 (67) ◽

pp. 39011-39024 ◽

Cited By ~ 1

Author(s):

Gargi Goswami ◽

Ratan Kumar ◽

Ankan Sinha ◽

Soumen Kumar Maiti ◽

Babul Chandra Dutta ◽

...

Keyword(s):

Low Cost ◽

Water Recycling ◽

Commercial Grade ◽

Harvesting Efficiency ◽

Harvesting Process

A low-cost and scalable microalgal harvesting process with high harvesting efficiency has been demonstrated using a commercial flocculant and spent-water recycling.

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New Natural Coagulant Aid in Water Treatment

MATEC Web of Conferences ◽

10.1051/matecconf/201820303004 ◽

2018 ◽

Vol 203 ◽

pp. 03004

Author(s):

Jenny Siew Lee Chew ◽

Agnes Yin Yee Ho ◽

Boon Chin Lim ◽

Wai Loon Chan ◽

Yeek Chia Ho ◽

...

Keyword(s):

The Other ◽

Acid Extraction ◽

Annona Muricata ◽

Natural Coagulant ◽

Operational Conditions ◽

Microalgae Harvesting ◽

Other Hand ◽

New Material ◽

Harvesting Efficiency ◽

Flocculation Process

Natural source of coagulant is certainly being considered in addressing the disadvantages associated with the use of inorganic coagulants. Annona muricata is hypothesized to be new material as natural coagulant aid in coagulation-flocculation process. On the other hand, due to the high lipids content in microalgae, namely, Chlorella vulgaris is used in producing renewable energy, i.e. biodiesel. Coagulation-flocculation is an effective method in microalgae harvesting. Thus, in this study, a novel natural coagulant aid extracted from fruit waste in algae harvesting. It is aimed to (1) to extract natural coagulant aid (hereafter is known as biopolymer) extracted from Annona muricata seeds in algae harvesting, and (2) to evaluate the operational conditions of coagulation-flocculation process by utilizing the biopolymer. As a result, it is observed that acid extraction and extraction through sodium chloride does not show any yield. On the other hand, the harvesting efficiency showed positive response as coagulant aid at 20 mg/L.

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An economic assessment of astaxanthin production by large scale cultivation of Haematococcus pluvialis

Biotechnology Advances ◽

10.1016/j.biotechadv.2011.04.001 ◽

2011 ◽

Vol 29 (6) ◽

pp. 568-574 ◽

Cited By ~ 175

Author(s):

Jian Li ◽

Daling Zhu ◽

Jianfeng Niu ◽

Songdong Shen ◽

Guangce Wang

Keyword(s):

Large Scale ◽

Haematococcus Pluvialis ◽

Economic Assessment ◽

Astaxanthin Production ◽

Large Scale Cultivation

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Automated Filtration-Based High-Throughput Plasmid Preparation System

Genome Research ◽

10.1101/gr.9.5.463 ◽

1999 ◽

Vol 9 (5) ◽

pp. 463-470 ◽

Cited By ~ 4

Author(s):

Masayoshi Itoh ◽

Tokuji Kitsunai ◽

Junichi Akiyama ◽

Kazuhiro Shibata ◽

Masaki Izawa ◽

...

Keyword(s):

High Throughput ◽

Large Scale ◽

Microtiter Plate ◽

Linear Process ◽

Alkaline Lysis ◽

Filtration Method ◽

Cell Harvesting ◽

Automated Processing ◽

Plasmid Preparation ◽

Plasmid Purification

Current methods of plasmid preparation do not allow for large capacity automated processing. We have developed an automated high-throughput system that prepares plasmid DNA for large-scale sequencing. This system is based on our previously reported filtration method. In this method, cell harvesting, alkaline lysis, and plasmid purification occur in a single 96-well microtiter plate from which sequence-ready DNA samples are collected. The plates are designed to allow all reagents to be injected from above the wells and the spent reagents to be aspirated from below. This design has enabled us to build a linear process plasmid preparation system consisting of an automated filter plate stacker and a 21-stage automated plasmid preparator. The 96-well plates used are outfitted with glass-filters that trap Escherichia coli before the plates are stacked in the automated stacker. The plates move from the stacker to each of the 21 stages of the preparator. At specific stages, various reagents or chemicals are injected into the wells from above. Finally, the plates are collected in the second stacker. The optimal throughput of the preparator is 40,000 samples in 17.5 hr. Here, we describe a pilot experiment preparing 15,360 templates in 160 specially designed 96-well glass-filter plates. The prepared plasmids were subjected to restriction digestion, DNA sequencing, and transcriptional sequencing.

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A new pneumatic harvester for improvement and facilitation the harvesting of the olive fruits

Advances in Horticultural Science ◽

10.36253/ahsc-9609 ◽

2021 ◽

Vol 35 (1) ◽

Author(s):

Fatemeh Zare ◽

Gholamhassan Najafi ◽

Teimour Tavakoli Hashjin ◽

Alimashallah M. Kermani ◽

Pedram Ghiasi

Keyword(s):

Block Design ◽

Variance Analysis ◽

Collector System ◽

Scope Test ◽

Randomized Complete Block Design ◽

Harvesting Efficiency ◽

Fruit Damage ◽

Harvesting Process

The objective of this study was to measure and evaluate the performance of a New Pneumatic Harvester (NPH) for harvesting the olives fruit and compare the results with those of similar cases. The study involves two sections, namely, the NPH and the collector system. Two oily types of olive (‘Mari’ and ‘Yellow’) were selected to evaluate the NPH and the collector system. The randomized complete block design and Duncan’s multi-scope test were used for variance analysis and means comparison purposes, respectively. The dependent parameters used to evaluate the NPH were harvesting productivity, harvesting efficiency, and leave downfall percent. Also, the fruit damage was selected for the collector system. Results showed that the NPH can harvest 92% of olive fruits. The highest amount of harvesting productivity belongs to NPH of 29.47 kg/h. The percent of leaves downfall in the harvesting process was found to be 2.55%. Using the collector system can reduce the level of damaged fruit from 60.8% to 25.12% and from 60.54% to 24.54% in the Mari and Yellow variety.

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The Growth Comparison of Haematococcus Pluvialis in Two Different Medium

Biota ◽

10.20414/jb.v12i2.202 ◽

2019 ◽

Vol 12 (2) ◽

Author(s):

Dina Soes Putri ◽

Siti Alaa

Keyword(s):

Growth Rate ◽

Culture Medium ◽

Haematococcus Pluvialis ◽

Marine Ecosystem ◽

Culture Conditions ◽

Vital Role ◽

Freshwater Microalgae ◽

Inoculum Concentration ◽

Chemical Factors ◽

Physical And Chemical

Microalgae is an aquatic microorganism that conducts photosynthesis. It plays a vital role as an oxygen producer in the marine ecosystem. A freshwater microalgae, Haematococcus pluvialis, has been utilized as a health supplement and industrial application which is beneficial for human. In addition to physical and chemical factors, nutrient composition is one crucial thing that contributes to the growth of microalgae. This present study aimed to determine and compare the growth rate of Haematococcus pluvialis cultivated in two culture medium, Walne’s and Guillard. The culture conditions observed were light intensity, photoperiod of light-dark hours, temperature, inoculum concentration of medium’s liquid, and cell density. This study confirmed that Walne’s media produced much higher biomass (247x104 cells/mL) than Guillard’s medium (209.6x104 cells/mL). The aspect to be further performed on H. pluvialis biomass is exploring its high-value bio compound.

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