The Anthelmintics Activity of Ethanol Extract of African Leaf (Vernonia amygdalina) on Mortality of Fasciola gigantica in Vitro

The Aims of this study is to know the anthelmintics activity of ethanol extract of african leaf (Vernonia amygdalina) on mortality of Fasciola gigantica in Vitro. Method that used in the research was a completely randomized design. There were five treatments and each treatment was done in four replications. This research used ten Fasciola gigantica in each treatment for all replications. The observation and recording of dead Fasciola gigantica was done at 2, 4, 6, 8, and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (500C). The obtained data was analyzed using ANOVA and continued with Duncan Multiple Range Test. The result of this research show that ethanol of extract of African Leaf (Vernonia amygdalina) has activity anthelmintic on mortality of Fasciola gigantica in Vitro. Ethanol extract african leaf (Vernonia amygdalina) with concentration of 1.4% is the optimal concentration of anthelmintics. This higher the concentration of the extract, the higher the property of anthelmintics.

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The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro

Journal of Parasite Science ◽

10.20473/jops.v3i1.16427 ◽

2019 ◽

Vol 3 (1) ◽

pp. 19

Author(s):

Amelia Dwita Safitri ◽

Iwan Sahrial Hamid ◽

Poedji Hastutiek ◽

Setiawan Koesdarto ◽

Rahmi Sugihartuti ◽

...

Keyword(s):

Anthelmintic Activity ◽

Ethanol Extract ◽

Probit Analysis ◽

Ascaridia Galli ◽

Vernonia Amygdalina ◽

Randomized Design ◽

Lethal Time ◽

Range Test ◽

Research Show

The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours.

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Anthelmintic Activity of Ocimum sanctum Linn. Leaves Ethanol Extract Against Fasciola gigantica in vitro

KnE Life Sciences ◽

10.18502/kls.v3i6.1135 ◽

2017 ◽

Vol 3 (6) ◽

pp. 266

Author(s):

Mesia Margi Mahardika ◽

Sri Agus Sudjarwo ◽

Setiawan Koesdarto

Keyword(s):

Exposure Time ◽

Anthelmintic Activity ◽

Ethanol Extract ◽

Probit Analysis ◽

Fasciola Gigantica ◽

Ocimum Sanctum ◽

Randomized Design ◽

Range Test ◽

Lc50 And Lc90

The aims of this research are to know the concentration, exposure time and interaction between concentration and exposure time of Ocimum sanctum Linn. leaves ethanol extract which cause the most mortality toward Fasciola gigantica. Also to know its value of LC50 and LC90. The research was completely randomized design. There were five treatments. Each treatment was done in four replications and used 10 Fasciola gigantica. The observation and recording of dead Fasciola gigantica were done at 0, 2, 4, 6, 8 and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (50ºC). The obtained data was analyzed using ANOVA Factorial and continued with Duncan Multiple Range Test. The result was 10% concentration and exposure time for 10 hours caused the most mortality toward Fasciola gigantica. However, the interaction between concentration and exposure time resulted that 5% concentration for 8 hours already caused the most mortality of Fasciola gigantica. Probit analysis was used to calculate the LC50 and LC90. The results were LC50 of Ocimum sanctum Linn. leaves ethanol extract was 7.9% at 4 hours, 3.7% at 6 hours, 1.8% at 8 hours and 0.8% at 10 hours and the LC90 was 8.4% at 10 hours. Key words: Ocimum sanctum Linn. leaves, Fasciola gigantica, ethanol extract, in vitro.

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Nutrient content evaluation of dried poultry waste urea molasses block (DPW-UMB) on In-vitro analysis

Sains Peternakan ◽

10.20961/sainspet.v16i2.21264 ◽

2018 ◽

Vol 16 (2) ◽

pp. 50

Author(s):

Danung Nur Adli ◽

Osfar Sjofjan

Keyword(s):

Crude Protein ◽

Nutrient Content ◽

Poultry Waste ◽

Duncan Multiple Range Test ◽

Randomized Design ◽

Completely Randomized Design ◽

Vitro Analysis ◽

Range Test ◽

In Vitro Analysis

The research purpose was to determine nutrient content of dried of poultry waste urea molasses block (DPW-UMB). The research method was used completely randomized design with 3 treatments and 5 replicates. The treatments used for research were T1 (10% manure of laying chicken and 25% molasses), T2 (15% manure of laying chicken and 30% molasses), and T3 (20% manure of laying chicken and 30% molasses). The data analysis was the analysis of variance (anova) and continued by Duncan Multiple Range Test. The results showed that treatments has significantly difference (P<0.01) on dry matter, crude protein, and ash. It could be concluded that dpw-umb contained sufficient levels of nutrients. it could be used as feedstuff for ruminants for supplementation with the required nutrients.

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The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro

Journal of Parasite Science ◽

10.20473/jops.v3i1.16429 ◽

2019 ◽

Vol 3 (1) ◽

pp. 23

Author(s):

Meta Aprilia ◽

Poedji Hastutiek ◽

Rochmah Kurnijasanti ◽

Lucia Tri Suwanti ◽

Mohammad Sukmanadi ◽

...

Keyword(s):

Ethanol Extract ◽

Probit Analysis ◽

Boophilus Microplus ◽

Lethal Concentration ◽

Betel Leaf ◽

Randomized Design ◽

Completely Randomized Design ◽

Research Show

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.

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Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

Jurnal Natur Indonesia ◽

10.31258/jnat.13.1.63-66 ◽

2012 ◽

Vol 13 (1) ◽

pp. 63

Author(s):

Taswin Yacob ◽

Rita Endriani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethanol Extract ◽

Positive Control ◽

Diffusion Method ◽

Randomized Design ◽

Senna Alata ◽

Treatment Of Infection ◽

Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

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Penggunaan Bahan Organik dan Kombinasinya dalam Formulasi Biofungisida Berbahan Aktif Jamur Trichoderma pseudokoningii Rifai. untuk Menghambat Jamur Ganoderma boninense Pat. secara in vitro

Jurnal Natur Indonesia ◽

10.31258/jnat.16.2.79-90 ◽

2017 ◽

Vol 16 (2) ◽

pp. 79

Author(s):

Yetti Elfina ◽

Muhammad Ali ◽

Rachmad Saputra

Keyword(s):

Rice Husk ◽

Ganoderma Boninense ◽

Organic Matters ◽

Randomized Design ◽

Shrimp Shell ◽

Trichoderma Pseudokoningii ◽

Completely Randomized Design ◽

Rot Disease ◽

Range Test

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.

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THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽

10.31315/agrivet.v27i1.4694 ◽

2021 ◽

Vol 27 (1) ◽

pp. 39

Author(s):

Salma Nabila ◽

Endah Budi Irawati ◽

Rina Srilestari

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Fresh Weight ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Range Test ◽

Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

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Kandungan Total Bakteri dan Total Fungi pada Pellet Limbah Penetasan yang Dibuat dengan Penambahan Bentonit

Jurnal Agripet ◽

10.17969/agripet.v16i2.3192 ◽

2016 ◽

Vol 16 (2) ◽

pp. 69

Author(s):

Aprilian Adi Nugroho ◽

Sri Sumarsih ◽

Bambang Sulistiyanto

Keyword(s):

Analysis Of Variance ◽

Total Content ◽

Duncan Multiple Range Test ◽

Randomized Design ◽

Completely Randomized Design ◽

Bacteria And Fungi ◽

Total Fungi ◽

Number Of Bacteria ◽

Total Bacteria ◽

Range Test

ABSTRAK. Penelitian efek penambahan bentonit dalam proses pelleting terhadap total bakteri dan total fungi dari produk pellet limbah penetasan sebagai bahan pakan alternatif, telah dilaksanakan di Laboratorium Teknologi Pakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro. Penambahan bentonit pada proses pelleting diharapkan mengurangi total bakteri dan total fungi pada pellet olahan limbah penetasan, sehingga aman digunakan sebagai bahan pakan alternatif. Pada penelitian ini limbah penetasan yang berupa cangkang telur, telur infertile, telur gagal menetas, serta DOC afkir dan mati dihancurkan, dicampur dengan bentonit dan dibuat pellet, untuk kemudian dianalisis kandungan total bakteri dan total fungi pada produk pellet. Perlakuan bentonit dilakukan dengan aras 0, 2, 4 dan 6% (B/B). Penelitian dilakukan dengan rancangan acak lengkap (RAL) 4 perlakuan dan 5 ulangan. Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji wilayah ganda Duncan untuk mengetahui perbedaan antar perlakuan. Hasil menunjukkan bahwa pemberian bentonit dari berbagai level berpengaruh sangat nyata (P<0,01). Pemberian bentonit pada tingkat tertinggi (6%) mampu menekan total bakteri dan total fungi pada kisaran 105 cfu/g. Disimpulkan bahwa bentonit mampu menekan kandungan total bakteri dan total fungi pada produk pellet limbah penetasan. (Numbers of total bacteria and total fungi of hatchery waste pellets that made by adding bentonite) ABSTRACT. A Research about the effect of bentonite addition in the pelletizing process on total bacteria and total fungi of pellet product of hatchery waste as an alternative feedstuff, was done at Feed Technology Laboratory, Faculty of Animal and Agricultural Sciences, Diponegoro University. The addition of bentonite was expected to reduce the number of bacteria and fungi in the pellet product of hatchery waste, therefore, it could be used safely as an alternative feedstuff. In this experiment, the hatchery waste in the forms of eggshell, infertile eggs, un-hatched eggs, dead and culled DOC were blended, mixed with bentonite and pelleted,and then the number of total bacteria and total fungi of pellet was analyzed. The treatment was carried out with the level of bentonite i.e. 0, 2, 4 and 6% (w/w). Completely randomized design (CRD)was applied by 4 treatments and 5 replications of each. Data analyzed by analysis of variance (ANOVA), and followed by Duncan multiple range test (DMRT) to determine differences among the treatments. The results showed that administration of bentonite at various levels significantly affect the number of bacteria and fungi in the hatchery waste pellets (P <0.01). Administration of bentonite at the level of 6% shows the highest value on suppressing the content of total bacteria as well as fungi up to the range of total content 105cfu/g. In conclusion, the bentonite was able to suppress the total amount of bacteria and total fungi in the hatchery waste pellets.

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Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations

Akta Agrosia ◽

10.31186/aa.22.1.29-35 ◽

2019 ◽

Vol 22 (1) ◽

pp. 29-35

Author(s):

Usman Kris Joko Suharjo ◽

Hasanudin Hasanudin ◽

Tunjung Pamekas ◽

Hesti Pujiwati ◽

Alyi Vanturini

Keyword(s):

Poor Quality ◽

Tuber Formation ◽

Potato Tubers ◽

Seed Sources ◽

Randomized Design ◽

Plant Culture ◽

Wet Weight ◽

Completely Randomized Design ◽

Range Test

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The research design used was Completely Randomized Design (CRD) which consist of 18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato

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Pemberian Ekstrak Daun Katuk (Sauropus androgynus L. Merr) dalam Air Minum Kambing Peranakan Ettawa untuk Meningkatkan Produksi dan Kualitas Susu

Jurnal Agripet ◽

10.17969/agripet.v18i2.11947 ◽

2018 ◽

Vol 18 (2) ◽

pp. 90-94

Author(s):

Dedhi Yustendi ◽

Ainal Mardhiah

Keyword(s):

Drinking Water ◽

Milk Production ◽

Leaf Extract ◽

Duncan Multiple Range Test ◽

Randomized Design ◽

Milk Volume ◽

Data Volume ◽

Completely Randomized Design ◽

Range Test ◽

Banda Aceh

ABSTRAK. Penelitian dilaksanakan pada kelompok ternak kambing perah di Desa Geucee Kayee Jatoe Kota Banda Aceh. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan persentase pemberian ekstrak daun katuk dalam air minum yaitu S0, S1, S2 dan S3 (0 %; 10%; 20 %, dan 30% ), tiap perlakuan diulang sebanyak 4 kali. Data volume produksi air susu yang diperoleh dianalisa dengan ANOVA, dan bila terdapat perbedaan, maka dilanjutkan dengan uji lanjut Duncan. Hasil penelitian menunjukkan pengaruh yang nyata (P0.05) terhadap volume air susu yang dihasilkan antar perlakuan S0, S1, S2 dan S3. Pemberian ekstrak daun katuk 10% (S1) dalam air minum memperlihatkan peningkatan produksi susu dan kualitas susu yang baik dibandingkan dengan pemberian 20% dan 30% dalam air minum. (Giving katuk leaf extract (Sauropus androgynus L. Merr) in drinking water Ettawa crossbreed goats to increase milk volume and milk quality) ABSTRACT. This research was carried out on a group of Ettawa crossbreed goats in Geucee Kayee Jatoe, Banda Aceh. This study used a completely randomized design with 4 (four) treatments of giving katuk leaves extract in drinking water, namely S0, S1, S2 and S3 (0%; 10%; 20%, and 30% of katuk leaf) and each group repeated 4 times. Data on milk production were analyzed by analysis of variant (ANOVA), and if there were differences, then followed by Duncan multiple range test (DMRT). The results showed that there was no significant effect (P 0.05) on the production of milk between treatments S0, S1, S2 and S3. Giving katuk leaf extract up to 10% (S1) in drinking water increase in milk production and quality compared to those giving 20% and 30% of katuk leaf.

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