Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The research design used was Completely Randomized Design (CRD) which consist of 18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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Penggunaan Bahan Organik dan Kombinasinya dalam Formulasi Biofungisida Berbahan Aktif Jamur Trichoderma pseudokoningii Rifai. untuk Menghambat Jamur Ganoderma boninense Pat. secara in vitro

Jurnal Natur Indonesia ◽

10.31258/jnat.16.2.79-90 ◽

2017 ◽

Vol 16 (2) ◽

pp. 79

Author(s):

Yetti Elfina ◽

Muhammad Ali ◽

Rachmad Saputra

Keyword(s):

Rice Husk ◽

Ganoderma Boninense ◽

Organic Matters ◽

Randomized Design ◽

Shrimp Shell ◽

Trichoderma Pseudokoningii ◽

Completely Randomized Design ◽

Rot Disease ◽

Range Test

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.

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THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽

10.31315/agrivet.v27i1.4694 ◽

2021 ◽

Vol 27 (1) ◽

pp. 39

Author(s):

Salma Nabila ◽

Endah Budi Irawati ◽

Rina Srilestari

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Fresh Weight ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Range Test ◽

Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

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Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

Asian Journal of Agriculture and Rural Development ◽

10.18488/journal.1005/2018.8.2/1005.2.178.187 ◽

2019 ◽

Vol 8 (2) ◽

pp. 178-187

Author(s):

Subandi M ◽

Arkhan Jannata ◽

Sofiya Hasani

Keyword(s):

Musa Acuminata ◽

Laboratory Research ◽

Independent Effect ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Range Test ◽

Growth Induction

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

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Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

Jurnal Ilmiah Inovasi ◽

10.25047/jii.v21i1.2636 ◽

2021 ◽

Vol 21 (1) ◽

pp. 54-57

Author(s):

Dyah Nuning Erawati ◽

Yusriatul Mawaddah ◽

Siti Humaida ◽

Irma Wardati

Keyword(s):

Tissue Culture ◽

Shoot Multiplication ◽

Culture Techniques ◽

Planting Material ◽

Randomized Design ◽

Wet Weight ◽

The Mean ◽

Completely Randomized Design ◽

Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

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Kecernaan In Vitro Jerami Padi Fermentasi Dengan Menggunakan Berbagai Level Inokulum Aspergillus niger dan Lactobacillus plantarum

Jurnal Pengembangan Penyuluhan Pertanian ◽

10.36626/jppp.v11i22.144 ◽

2015 ◽

Vol 11 (22) ◽

pp. 25

Author(s):

Saputro , R. A. T. W ◽

Ngadiyono, N. ◽

Yusiati, L. M ◽

Budisatria, I. G. S.

Keyword(s):

Aspergillus Niger ◽

Lactobacillus Plantarum ◽

Randomized Design ◽

Completely Randomized Design ◽

Range Test

Pemanfaatan sisa hasil pertanian (jerami padi) sebagai pakan dasarmenjadi hal yang strategis untuk dikembangkan guna memenuhi kebutuhan pakan berserat bagi ternak sapi potong. Mikrobia selulolitik dan bakteri asam laktat merupakan salah satu sumber inokulum yang dapat meningkatkan kualitas jerami padi sebagai pakan dasar berserat. Penelitian dilakukan dengan tujuan untuk mendapatkan sumber karbon yang tepat untuk pertumbuhan Aspergillus niger(AN) dan Lactobacillus plantarum(LP).Penelitian ini dilakukan dengan menfermentasi jerami padi menggunakan AN dan LP. Perlakuan sumberkarbon yang diberikan dua macam substrat, yaitu molasses dan dedak. Perlakuan level AN sebesar 0, 5, 10, dan 15%. Pemberian LP sebesar 10% pada setiap perlakuan. Fermentasi dilakukan selama 21 hari,sedang nilai kecernaannyadievaluasi dengan menggunakan metode in vitro Tilley andTerrydan Gas Test. Variabel yang diamati dalam penelitian ini adalah pH, asam laktat,BK, BO, PK, SK, NDF, ADF, dan TDN. Data yang diperoleh dianalisis menggunakan analisis varian rancangan acak lengkap (Completely Randomized Design/CRD) pola searah dan pola faktoril (2 x 3), bila terdapat pengaruh nyata dilanjutkan dengan uji DMRT (Duncan’s Multiple Range Test). Hasil penelitian menunjukkan penggunaan Aspergillus nigerdan Lactobacillus plantarumuntuk fermentasi jerami padi yang terbaik adalah 15% Aspergillus nige rdan 10% Lactobacillus plantarum dari bahan kering. Penggunaan substrat molases lebih baik jika dibandingkan dengan dedak, hal ini terlihat dari hasil analisis PK, SK, LK, ADF, dan hasil uji fisik jerami padi fermentasi.

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Respon Pemberian Hormon 2,4-D dan BAP terhadap Pertumbuhan Subkultur Kalus Kedelai (Glycine max (L.) Merrill) secara In Vitro

Biosfera ◽

10.20884/1.mib.2015.32.1.296 ◽

2015 ◽

Vol 32 (1) ◽

pp. 59

Author(s):

Kiki Ayuningrum ◽

Iman Budisantoso ◽

Kamsinah Kamsinah

Keyword(s):

Glycine Max ◽

Combination Treatment ◽

Dry Weight ◽

Soybean Callus ◽

Randomized Design ◽

Glycine Max L ◽

Wet Weight ◽

Completely Randomized Design ◽

Callus Type

The purpose of this study was to evaluate the response of administration of a combination of 2,4-D and BAP on the growth of soybean callus subculture and determine the combination of 2,4-D and BAP most good for the growth of soybean callus subculture. The study design used completely randomized design (CRD) with a pattern factorials. The factor one e.i 2,4-D consists of 4 levels, namely: 0, 5, 10, and 15 ppm. A factor of 2 e.i BAP consists of 4 levels, namely: 0, 2, 4, dan 6 ppm. Every combination treatment repeated three times. Parameters measured include the percentage is growing callus, type of callus, dry weight and wet weight of soybean callus. The results showed that administration of the hormone 2,4-D and BAP can spur the growth of soybean callus subculture, the combination of BAP 2 ppm and 10 ppm of 2,4-D is the best combination for a percentage of callus and growing callus types, whereas the wet weight and the weight dried callus is not driven by a combination of the hormone 2,4-D and BAP

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Peningkatan Kualitas Jerami Padi MenggunakanTeknologi Amoniasi Fermentasi

Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology ◽

10.29303/jitpi.v2i1.18 ◽

2019 ◽

Vol 2 (1) ◽

pp. 96

Author(s):

Muhamad Amin ◽

Sofyan Damrah Hasan ◽

Oscar Yanuarianto ◽

Mohammad Iqbal ◽

I Wayan Karda

Keyword(s):

Organic Matter ◽

Crude Protein ◽

Dry Matter ◽

Crude Fiber ◽

In Vitro Digestibility ◽

Randomized Design ◽

Result Show ◽

Completely Randomized Design ◽

Range Test

An experiment with aim to study the effect of probiotics combination level and length of fermentation on qualityimprovement of rice straw had been conducted at the Laboratory of Nutrition and Feed of Animal HusbandryFaculty, Mataram University. The research was arranged in a Completely Randomized Design in factorialarrangement 3 x 3 with 3 replications. The first factor was the level of probiotics (0, 2 and 4%), the second factorwas the period of fermentation (2, 4 and 6 weeks).The variables measured were crude protein, crude fiber, NDF,ADF, cellulose, hemicellulose, lignin, and in vitro digestibility of dry matter and organic matter. Data wereanalyzed by analysis of variance and tested further by Duncan's Multiple Range Test. The result show thattreatments combination of the probiotics level and fermentation period did not affect (P>0.05) the chemicalcomposition and digestibility. The level of probiotics increased (P<0.05) the crude protein and in vitro dry matterdigestibility. Meanwhile, the lenght of fermentation increased the crude protein and in vitro digestibility of drymatter and organic matter, but decreased the crude fiber, NDF, ADF, cellulose, and hemicellulose of Amofer ricestraw.

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Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v22i1.36007 ◽

2020 ◽

Vol 22 (1) ◽

pp. 39

Author(s):

Amalina Wahyuni ◽

Benni Satria ◽

Aprizal Zainal

Keyword(s):

Tissue Culture ◽

Callus Induction ◽

Economic Value ◽

Market Demand ◽

Compact Structure ◽

Randomized Design ◽

Aquilaria Malaccensis ◽

Completely Randomized Design ◽

Range Test

<p>Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact. Yellow callus and compact structure could be utilized for organogenesis.</p>

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The Anthelmintics Activity of Ethanol Extract of African Leaf (Vernonia amygdalina) on Mortality of Fasciola gigantica in Vitro

Journal of Parasite Science ◽

10.20473/jops.v3i1.16426 ◽

2019 ◽

Vol 3 (1) ◽

pp. 15

Author(s):

Dhio Asmaydo ◽

Iwan Sahrial Hamid ◽

Muchammad Yunus ◽

Kusnoto Kusnoto ◽

Mohammad Sukmanadi ◽

...

Keyword(s):

Ethanol Extract ◽

Fasciola Gigantica ◽

Duncan Multiple Range Test ◽

Vernonia Amygdalina ◽

In Vitro Method ◽

Randomized Design ◽

Completely Randomized Design ◽

Range Test ◽

Research Show

The Aims of this study is to know the anthelmintics activity of ethanol extract of african leaf (Vernonia amygdalina) on mortality of Fasciola gigantica in Vitro. Method that used in the research was a completely randomized design. There were five treatments and each treatment was done in four replications. This research used ten Fasciola gigantica in each treatment for all replications. The observation and recording of dead Fasciola gigantica was done at 2, 4, 6, 8, and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (500C). The obtained data was analyzed using ANOVA and continued with Duncan Multiple Range Test. The result of this research show that ethanol of extract of African Leaf (Vernonia amygdalina) has activity anthelmintic on mortality of Fasciola gigantica in Vitro. Ethanol extract african leaf (Vernonia amygdalina) with concentration of 1.4% is the optimal concentration of anthelmintics. This higher the concentration of the extract, the higher the property of anthelmintics.

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