Pengaruh Pemberian Ekstrak Jintan Hitam (Nigella sativa) terhadap Kadar MDA dan SOD Hepar Tikus (Rattus norvegicus) yang Diinduksi Piroxicam

Exposure to ultraviolet (UV) radiation from the sun may cause photo aging which is characterized by uneven pigmentation and brown spots. Melanin acts to protect the skin against DNA damage due to UV radiation. Sunscreen is a cosmetic preparation that can reflect sunlight, especially in areas with UV wave emulsions. This study aims to determine the effect of black cumin extract cream on the amount of melanin pigment in rat skin exposed to ultraviolet-B rays. An experimental post-test only control group study was conducted from October 2019 - February 2020. A total of 30 rats were divided into 6 groups, namely the KN group (without treatment), P1 (basic cream), P2 (black cumin extract cream 0.25% ), P3 (0.5% cream), P4 (0.75% cream) and KP (Parasol sunscreen cream). Rats in group P4 showed significantly lower amounts of melanin (41.5 ± 10.4) than other groups (KN 41.5, P1 86.0, P2 84.7 and P3 78.7; p <0.05). However, the least amount of melanin pigment was found in the KP group (10.0 ± 1.82). It can be concluded that the topical black cumin extract cream can topically reduce the amount of melanin pigment.

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Efek Pemberian Ekstrak Biji Jintan Hitam (Nigella Sativa) Terhadap Konsentrasi GLUT-4 Pada Tikus Putih (Rattus Norvegicus) Strain Wistar Model Diabetes Melitus Tipe 2

Jurnal Ilmiah Kesehatan Media Husada ◽

10.33475/jikmh.v5i1.84 ◽

2016 ◽

Vol 5 (1) ◽

pp. 01-10

Author(s):

Efta Triastusi ◽

Meutia Tamimi Auli ◽

Ema Pristi Yunita

Keyword(s):

Rattus Norvegicus ◽

Nigella Sativa ◽

Tween 80 ◽

Glut 4 ◽

Tnf Α ◽

Diabetes Melitus

Diabetes Melitus (DM) merupakan masalah yang banyak terjadi di negara berkembang, salah satunya adalah di Indonesia.DM tipe 2 ditandai dengan keadaan resistensi insulin yang dapat terjadi karena penurunan sintesis dan translokasi GLUT-4 ke permukaan sel, hal tersebut disebabkan oleh hambatan aktivitas AMPK di jaringan otot karena produksi TNF-α yang berlebihan. Biji jintan hitam mengandung thymoquinone yang memiliki aktivitas sebagai antiinflamasi. Penelitian ini bertujuan untuk mengetahui efek ekstrak biji jintan hitam terhadap konsentrasi GLUT-4 di jaringan otot. Desain penelitian ini adalah true experimentaldengan sampel 25 ekor tikus putih jantan strain Wistar model DM tipe 2 yang dibagi ke dalam 5 kelompok yaitu kontrol negatif (10% tween 80), kontrol positif (metformin 75 mg/kgBB), 3 kelompok mendapat ekstrak biji jintan hitam dengan dosis yang berbeda (24 mg/kgBB, 48 mg/kgBB, dan 96 mg/kgBB). Induksi DM tipe 2 dilakukan selama 2 bulan dengan menggunakan pakan tinggi kalori yang mengandung 10% lemak babi dan 20% sukrosa serta injeksi STZ 30 mg/kgBB, lalu dilakukan terapi selama 1 bulan. Konsentrasi GLUT-4 diukur dengan menggunakan ELISA, hasilnya tidak terdapat perbedaan konsentrasi GLUT-4 yang signifikan antar kelompok (ANOVA, p > 0,05).

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PENGARUH PEMBERIAN EKSTRAK HABBATUSSAUDA (Nigella sativa) TERHADAP KADAR GLUKOSA DARAH TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR JANTAN YANG DIINDUKSI ALOKSAN SEBAGAI UPAYA PREVENTIF HIPERGLIKEMIA

PREPOTIF : Jurnal Kesehatan Masyarakat ◽

10.31004/prepotif.v5i1.1590 ◽

2021 ◽

Vol 5 (1) ◽

pp. 408-414

Author(s):

Yesi Nurmalasari ◽

Rakhmi Rafie ◽

Efrida Warganegara ◽

Indah Mulia Herwisdiane

Keyword(s):

Rattus Norvegicus ◽

Nigella Sativa ◽

T Test ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Post Test ◽

Paired T Test ◽

Diabetes Melitus

Latar Belakang:Hiperglikemia adalah suatu kondisi medis berupa peningkatan kadar glukosa darah melebihi kadar normal yang menjadi karakteristik beberapa penyakit terutama diabetes melitus. Salah satu cara untuk menurunkan kadar glukosa darah yaitu dengan ekstrak alami tanaman habbatussauda (Nigella sativa). Habbatussauda (Nigella sativa) mengandung thymoquinone yang dapat meningkatkan sensitivitas insulin di dalam jaringan tubuh dan juga dapat memperbaiki kerusakan sel-β pankreas sehingga meningkatkan sekresi insulin. Tujuan:Mengetahui pengaruh pemberian ekstrak habbatussauda (Nigella sativa) terhadap kadar glukosa darah tikus putih (Rattus norvegicus) galur Wistar jantan yang diinduksi aloksan sebagai upaya preventif hiperglikemia. Metode : Penelitian eksperimental murni pre and post test with control group design. Sampel yang digunakan sebanyak 25 ekor tikus jantan dibagi menjadi 5 kelompok. Hasil : Nilai rerata ± SD GDP sebelum perlakuan, setelah diinduksi aloksan dan setelah perlakuan pada KM (126 ± SD 3,53), (128 ± SD 3,24), dan (124,6 ± SD 8,47), pada KN (121,6 ± SD 7,12), (182,2 ± SD 18,11), dan (149 ± SD 14,90), pada KP (122 ± SD 3,536), (161,8 ± SD 6,76), dan (108,4 ± SD 9,52), pada P1 (123,80 ± SD 4,65), (139,8 ± SD 1,48), dan (100,8 ± SD 5,40), dan pada P2 (122,6 ±SD 3,36), (164 ±SD 9,13), dan (112,2 ± SD 4,71). Uji Paired T-test menunjukkan peningkatan kadar glukosa darah yang bermakna (p

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EFEK HEPATOPROTEKTOR EKSTRSAK JINTAN HITAM (Nigella sativa) TERHADAP KERUSAKAN HEPAR TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR Sparague Dawley YANG DIINDUKSI ETANOL

JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan ◽

10.22437/jmj.v6i1.4819 ◽

2018 ◽

Vol 6 (1) ◽

pp. 36-44

Author(s):

Rizky Rafiqoh Afdin ◽

Fairuz Quzwain

Keyword(s):

Normal Control ◽

Rattus Norvegicus ◽

Nigella Sativa ◽

Control Group ◽

Sprague Dawley ◽

Control Group Design ◽

Black Cumin ◽

Group Design ◽

White Rats ◽

Post Test

ABSTRACT Background: Among the various medicinal plants, black cumin (Nigella sativa) appears as an herb with a historical and religious background due to a lot of research. One of the most important effects on Nigella sativa is the hepatoprotective that has been shed in various studies. Thymoquinone which is the content of Nigella sativa has the ability to inhibit lipid peroxidation. Thymoquinone can reduce oxidative stress and increase antioxidants in the body. The objectives of this study were to overcome the effects hepatoprotector of black cumin extract (Nigella sativa) on the damage of hepatic of male white rat (Rattus norvegicus) sparague dawley strain induced by ethanol. Methodology: It is a kind of experimental laboratory research used that uses random controlled design method with the pattern of post test-only control group design. The subject of this research is 25 adult male white rats (Rattus norvegicus) Sparague Dawley strain aged 3-4 months, weight 150-300 grams, that are divided in to five groups. Group one (normal control) is given aquades dose 0,01 ml/grBB/day, group two (pathologic control) is given ethanol with dose o,o1 ml/grBB/day, group three is given 25% concentration of black cumin extract and 50% ethanol, group four is given 37,5% concentration of black cumin extract and 50% ethanol, and group five is given 50% concentration of black cumin extract and 50% ethanol. After 14th days, the treatment was stopped and the rats got dislocation cervical, and then laparotomy for made histopathology preparation. After getting observed, the data that is gotten is analyzed with Kruskal-Wallis test and then Mann-Whitney test. Result: The result of Kruskal-Wallis test showed that there was an effect of black cumin extract (Nigella sativa) on the damage of hepatic of male white rat (Rattus norvegicus) sparague dawley strain induced by ethanol with p = 0,001 (p <0,05) . Mann Whitney test show there is a means difference between group I (normal control) with group II (pathologic control), group III (dose I), group IV (dose II) and group V (dose III). Conclusion: There is a hepatoprotector effect of black cumin (Nigella sativa) extract to liver’s damage of white rats (Rattus norvegicus) sparague dawley strain induced by ethanol. Key words: Black cumin extract, ethanol, fatty degeneration of liver rats. ABSTRAK Latar Belakang: Di antara berbagai tanaman obat, jintan hitam (Nigella sativa) muncul sebagai ramuan dengan latar belakang sejarah dan agama karena banyak penelitian mengungkapkan potensi farmakologis dari tanaman tesebut. Salah satu efek yang paling penting pada Nigella sativa adalah hepatoprotektif yang ditelah dijelaskan dalam berbagai penelitian. Thymoquinone yang merupakan kandungan jintan hitam memiliki kemampuan untuk menghambat peroksidasi lipid. Thymoquinone dapat mengurangi stress oksidatif dan meningkatkan pertahanan antioksidan dalam tubuh. Tujuan penelitian ini untuk meneliti efek hepatoprotektor jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur Sprague Dawley yang diinduksi etanol. Metode: Jenis penelitian ini adalah eksperimental laboratorik menggunakan metode rancangan acak terkontrol dengan pola post test-only control group design. Subjek penelitian adalah 25 ekor tikus putih (Rattus norvegicus) jantan galur sparague dawley berumur 3-4 bulan, berat badan 150-300 gram, yang dibagi menjadi 5 kelompok. Kelompok I (kontrol normal) diberi aquades dosis 0,01 ml/grBB/hari, kelompok II (kontrol patologis) diberi etanol 50% dosis 0,01 ml/grBB/hari, kelompok III diberi ekstrak jintan hitam konsentrasi 25% dan etanol 50%, kelompok IV diberi ekstrak jintan hitam konsentrasi 37,5% dan etanol 50%, serta kelompok V diberi ekstrak jintan hitam konsentrasi 50% dan etanol 50%. Setelah hari ke-14, perlakuan dihentikan, dilakukan dislokasi pada leher tikus kemudian dilakukan laparotomi untuk pengambilan hepar tikus untuk dibuat preparat histopatologis. Setelah diamati, data dianalisis dengan uji Kruskal-Wallis dan dilanjutkan dengan uji Mann Whitney. Hasil: Hasil uji Kruskal-Wallis menunjukkan terdapat pengaruh ekstrak jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur sparague dawley yang diinduksi etanol, dengan nilai p = 0,001 (p<0,05). Uji Mann Whitney menunjukkan perbandingan yang bermakna didapatkan pada kelompok I (kontrol normal) dengan kelompok II (kontrol patologis), kelompok III (dosis I), kelompok IV (dosis II) dan kelompok V (dosis III). Kesimpulan: Terdapat efek hepatoprotektor ekstrak jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur Sprague Dawley yang diinduksi etanol. Kata kunci: ekstrak jintan hitam, etanol, degenerasi lemak hepar tikus.

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Pengaruh Pemberian Ekstrak Biji Jintan Hitam terhadap Kadar Kolesterol HDL dan LDL Rattus Norvegicus Jantan Galur Wistar yang Diberi Diet Tinggi Lemak

Hang Tuah Medical journal ◽

10.30649/htmj.v18i2.168 ◽

2021 ◽

Vol 18 (2) ◽

pp. 204

Author(s):

RETNO SETYA KEMALA ◽

CHOESNAN EFFENDI ◽

ASAMI RIETA KUMALA

Keyword(s):

Rattus Norvegicus ◽

Ldl Cholesterol ◽

Nigella Sativa ◽

Seed Extract ◽

Control Group ◽

Black Cumin ◽

Cholesterol Levels ◽

White Rats ◽

Black Cumin Seed ◽

Cumin Seed

There are a lot of various kinds of plants such as Black Cumin (Nigella sativa L.) which is believed by the people to increase HDL and decrease LDL levels in the blood.The purpose of this experiment to know the effect of extract black cumin seeds (Nigella sativa L.)can increase HDL and decrease LDL cholesterol levels of white rats (Rattus norvegicus).This experiment using 24 of rats which divided into 4 groups, namely the negative control group, positive control group, the treatment group one dosage 126 mg/KgBWand the treatment group two dosage 252 mg/KgBW. This research was carried out for 28 days and continued by termination.In the One way ANOVA test, HDL levels obtained significant values of 0.134 and LDL levels of 0.070 which means p> 0.05 and showed that black cumin seed extract (Nigella sativa L.) there’s no different.The conclusion of the experiment is giving black cumin seed extract (Nigella sativa L.) had no effect for increasing HDL and decreasing LDL cholesterol levels of white rats (Rattus norvegicus).Keywords: black cumin seed extract, HDL cholesterol levels, LDL cholesterol level, (Nigella sativa L.).

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PENGARUH KRIM EKSTRAK JINTAN HITAM (Nigella sativa) TERHADAP KADAR KOLAGEN DAN HIDRASI KULIT PADA TIKUS (Rattus norvegicus) GALUR WISTAR JANTAN YANG DIPAPAR SINAR ULTRAVIOLET-B

BERITA BIOLOGI ◽

10.14203/beritabiologi.v19i3a.3863 ◽

2020 ◽

Vol 19 (3A) ◽

Author(s):

Winda Sari ◽

Linda Chiuman ◽

Sahna Ferdinand Ginting ◽

Chrismis Novalinda Ginting

Keyword(s):

Rattus Norvegicus ◽

Nigella Sativa ◽

Seed Extract ◽

Ultraviolet B ◽

Oxygen Species ◽

Black Cumin ◽

Wistar Strain ◽

Before And After ◽

Black Cumin Seed ◽

Cumin Seed

Photoaging is caused by exposure to sunlight and ultraviolet radiation (UV-B) through the reactive oxygen species (ROS) molecules formation, resulting in decreased levels of collagen. It is characterized by wrinkles, sagging and rough surfaces. Black cumin has been used in medicine due to its high antioxidant potential. The aim of this study is to identify the effect of black cumin cream on collagen levels and hydration of the skin of Rattus norvegicus exposed to ultraviolet-B rays. This is experimental study in which 30 male Wistar strain rats were classified into 6 sub-groups, namely the KN group (without treatment), KP (getting Nivea cream moisturizer), P1 (basic cream ), P2 (black cumin seed extract cream 0.25%), P3 (cream 0.5%) and P4 (cream 0.75%). P4 rat collagen levels increased significantly before and after the intervention (24 ± 2.23 vs 42.6 ± 14.6, p <0.05).Cream with lower concentrations did not provide a significant increase (p> 0.05). P4 rats also showed increased skin hydration (38.6 ± 19.3 to 63.0 ± 4.47 (p <0.05)), and this was not found in other groups. The administration of topical black cumin seed extract 0.75% can inhibit decreased levels of collagen and increase skin tissue hydration.

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