scholarly journals The effect of black cumin (Nigella sativa) extract on spermatozoa morphology of male albino rats (Rattus norvegicus) after induced with 2-Methoxyethanol

2019 ◽  
Vol 1246 ◽  
pp. 012043
Author(s):  
Indah Rahmadaniah ◽  
Salni ◽  
Joko Marwoto ◽  
Sagita Darmasari
Keyword(s):  
Rattus Norvegicus ◽  
Nigella Sativa ◽  
Albino Rats ◽  
Black Cumin

scholarly journals The Effect of Black Cumin Extract Cream (Nigella Sativa) On Melanin Pigment Level In Rats (Rattus Norvegicus) Exposed With Ultraviolet-B rays

2020 ◽  
Vol 11 (4) ◽  
pp. 5221-5224
Author(s):  
Yuli Marlina Tobing ◽  
Linda Chiuman ◽  
Sahna Ferdinand Ginting ◽  
Chrismis Novalinda Ginting
Keyword(s):  
Uv Radiation ◽  
Rattus Norvegicus ◽  
Nigella Sativa ◽  
Ultraviolet B ◽  
Melanin Pigment ◽  
Control Group ◽  
The Sun ◽  
Rat Skin ◽  
Black Cumin ◽  
Post Test

Exposure to ultraviolet (UV) radiation from the sun may cause photo aging which is characterized by uneven pigmentation and brown spots. Melanin acts to protect the skin against DNA damage due to UV radiation. Sunscreen is a cosmetic preparation that can reflect sunlight, especially in areas with UV wave emulsions. This study aims to determine the effect of black cumin extract cream on the amount of melanin pigment in rat skin exposed to ultraviolet-B rays. An experimental post-test only control group study was conducted from October 2019 - February 2020. A total of 30 rats were divided into 6 groups, namely the KN group (without treatment), P1 (basic cream), P2 (black cumin extract cream 0.25% ), P3 (0.5% cream), P4 (0.75% cream) and KP (Parasol sunscreen cream). Rats in group P4 showed significantly lower amounts of melanin (41.5 ± 10.4) than other groups (KN 41.5, P1 86.0, P2 84.7 and P3 78.7; p <0.05). However, the least amount of melanin pigment was found in the KP group (10.0 ± 1.82). It can be concluded that the topical black cumin extract cream can topically reduce the amount of melanin pigment.

EFEK HEPATOPROTEKTOR EKSTRSAK JINTAN HITAM (Nigella sativa) TERHADAP KERUSAKAN HEPAR TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR Sparague Dawley YANG DIINDUKSI ETANOL

2018 ◽  
Vol 6 (1) ◽  
pp. 36-44
Author(s):  
Rizky Rafiqoh Afdin ◽  
Fairuz Quzwain
Keyword(s):  
Normal Control ◽  
Rattus Norvegicus ◽  
Nigella Sativa ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Black Cumin ◽  
Group Design ◽  
White Rats ◽  
Post Test

ABSTRACT Background: Among the various medicinal plants, black cumin (Nigella sativa) appears as an herb with a historical and religious background due to a lot of research. One of the most important effects on Nigella sativa is the hepatoprotective that has been shed in various studies. Thymoquinone which is the content of Nigella sativa has the ability to inhibit lipid peroxidation. Thymoquinone can reduce oxidative stress and increase antioxidants in the body. The objectives of this study were to overcome the effects hepatoprotector of black cumin extract (Nigella sativa) on the damage of hepatic of male white rat (Rattus norvegicus) sparague dawley strain induced by ethanol. Methodology: It is a kind of experimental laboratory research used that uses random controlled design method with the pattern of post test-only control group design. The subject of this research is 25 adult male white rats (Rattus norvegicus) Sparague Dawley strain aged 3-4 months, weight 150-300 grams, that are divided in to five groups. Group one (normal control) is given aquades dose 0,01 ml/grBB/day, group two (pathologic control) is given ethanol with dose o,o1 ml/grBB/day, group three is given 25% concentration of black cumin extract and 50% ethanol, group four is given 37,5% concentration of black cumin extract and 50% ethanol, and group five is given 50% concentration of black cumin extract and 50% ethanol. After 14th days, the treatment was stopped and the rats got dislocation cervical, and then laparotomy for made histopathology preparation. After getting observed, the data that is gotten is analyzed with Kruskal-Wallis test and then Mann-Whitney test. Result: The result of Kruskal-Wallis test showed that there was an effect of black cumin extract (Nigella sativa) on the damage of hepatic of male white rat (Rattus norvegicus) sparague dawley strain induced by ethanol with p = 0,001 (p <0,05) . Mann Whitney test show there is a means difference between group I (normal control) with group II (pathologic control), group III (dose I), group IV (dose II) and group V (dose III). Conclusion: There is a hepatoprotector effect of black cumin (Nigella sativa) extract to liver’s damage of white rats (Rattus norvegicus) sparague dawley strain induced by ethanol. Key words: Black cumin extract, ethanol, fatty degeneration of liver rats.   ABSTRAK Latar Belakang: Di antara berbagai tanaman obat, jintan hitam (Nigella sativa) muncul sebagai ramuan dengan latar belakang sejarah dan agama karena banyak penelitian mengungkapkan potensi farmakologis dari tanaman tesebut. Salah satu efek yang paling penting pada Nigella sativa adalah hepatoprotektif yang ditelah dijelaskan dalam berbagai penelitian. Thymoquinone yang merupakan kandungan jintan hitam memiliki kemampuan untuk menghambat peroksidasi lipid. Thymoquinone dapat mengurangi stress oksidatif dan meningkatkan pertahanan antioksidan dalam tubuh.  Tujuan penelitian ini untuk meneliti efek hepatoprotektor jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur Sprague Dawley yang diinduksi etanol. Metode: Jenis penelitian ini adalah eksperimental laboratorik menggunakan metode rancangan acak terkontrol dengan pola post test-only control group design. Subjek penelitian adalah 25 ekor tikus putih (Rattus norvegicus) jantan galur sparague dawley berumur 3-4 bulan, berat badan 150-300 gram, yang dibagi menjadi 5 kelompok. Kelompok I (kontrol normal) diberi aquades dosis 0,01 ml/grBB/hari, kelompok II (kontrol patologis) diberi etanol 50% dosis 0,01 ml/grBB/hari, kelompok III diberi ekstrak jintan hitam konsentrasi 25% dan etanol 50%, kelompok IV diberi ekstrak jintan hitam konsentrasi 37,5% dan etanol 50%, serta kelompok V diberi ekstrak jintan hitam konsentrasi 50% dan etanol 50%. Setelah hari ke-14, perlakuan dihentikan, dilakukan dislokasi pada leher tikus kemudian dilakukan laparotomi untuk pengambilan hepar tikus untuk dibuat preparat histopatologis. Setelah diamati, data dianalisis dengan uji Kruskal-Wallis dan dilanjutkan dengan uji Mann Whitney. Hasil: Hasil uji Kruskal-Wallis menunjukkan terdapat pengaruh ekstrak jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur sparague dawley yang diinduksi etanol, dengan nilai p = 0,001 (p<0,05). Uji Mann Whitney menunjukkan perbandingan yang bermakna didapatkan pada kelompok I (kontrol normal) dengan kelompok II (kontrol patologis), kelompok III (dosis I), kelompok IV (dosis II) dan kelompok V (dosis III). Kesimpulan: Terdapat efek hepatoprotektor ekstrak jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur Sprague Dawley yang diinduksi etanol.   Kata kunci: ekstrak jintan hitam, etanol, degenerasi lemak hepar tikus.

scholarly journals Pengaruh Pemberian Ekstrak Biji Jintan Hitam terhadap Kadar Kolesterol HDL dan LDL Rattus Norvegicus Jantan Galur Wistar yang Diberi Diet Tinggi Lemak

2021 ◽  
Vol 18 (2) ◽  
pp. 204
Author(s):  
RETNO SETYA KEMALA ◽  
CHOESNAN EFFENDI ◽  
ASAMI RIETA KUMALA
Keyword(s):  
Rattus Norvegicus ◽  
Ldl Cholesterol ◽  
Nigella Sativa ◽  
Seed Extract ◽  
Control Group ◽  
Black Cumin ◽  
Cholesterol Levels ◽  
White Rats ◽  
Black Cumin Seed ◽  
Cumin Seed

<p>There are a lot of various kinds of plants such as Black Cumin <em>(Nigella sativa L.)</em> which is believed by the people to increase HDL and decrease LDL levels in the blood.</p><p>The purpose of this experiment<strong> </strong>to know the effect of extract black cumin seeds <em>(Nigella sativa L.)</em>can increase HDL and decrease LDL cholesterol levels of white rats <em>(Rattus norvegicus)</em>.</p><p>This experiment using 24 of rats which divided into 4 groups, namely the negative control group, positive control group, the treatment group one dosage 126 mg/KgBWand the treatment group two dosage 252 mg/KgBW. This research was carried out for 28 days and continued by termination.</p><p>In the One way ANOVA test, HDL levels obtained significant values of 0.134 and LDL levels of 0.070 which means p&gt; 0.05 and showed that black cumin seed extract (Nigella sativa L.) there’s no different.</p><p>The conclusion of the experiment is giving black cumin seed extract <em>(Nigella sativa L.) </em>had no effect for increasing HDL and decreasing LDL cholesterol levels of white rats <em>(Rattus norvegicus)</em>.</p><p><strong>Keywords: </strong> black cumin seed extract, HDL cholesterol levels, LDL cholesterol level, <em>(Nigella sativa L.)</em>.</p><p><strong> </strong></p>

scholarly journals PENGARUH KRIM EKSTRAK JINTAN HITAM (Nigella sativa) TERHADAP KADAR KOLAGEN DAN HIDRASI KULIT PADA TIKUS (Rattus norvegicus) GALUR WISTAR JANTAN YANG DIPAPAR SINAR ULTRAVIOLET-B

2020 ◽  
Vol 19 (3A) ◽  
Author(s):  
Winda Sari ◽  
Linda Chiuman ◽  
Sahna Ferdinand Ginting ◽  
Chrismis Novalinda Ginting
Keyword(s):  
Rattus Norvegicus ◽  
Nigella Sativa ◽  
Seed Extract ◽  
Ultraviolet B ◽  
Oxygen Species ◽  
Black Cumin ◽  
Wistar Strain ◽  
Before And After ◽  
Black Cumin Seed ◽  
Cumin Seed

Photoaging is caused by exposure to sunlight and ultraviolet radiation (UV-B) through the reactive oxygen species (ROS) molecules formation, resulting in decreased levels of collagen. It is characterized by wrinkles, sagging and rough surfaces. Black cumin has been used in medicine due to its high antioxidant potential. The aim of this study is to identify the effect of black cumin cream on collagen levels and hydration of the skin of Rattus norvegicus exposed to ultraviolet-B rays. This is experimental study in which 30 male Wistar strain rats were classified into 6 sub-groups, namely the KN group (without treatment), KP (getting Nivea cream moisturizer), P1 (basic cream ), P2 (black cumin seed extract cream 0.25%), P3 (cream 0.5%) and P4 (cream 0.75%). P4 rat collagen levels increased significantly before and after the intervention (24 ± 2.23 vs 42.6 ± 14.6, p <0.05).Cream with lower concentrations did not provide a significant increase (p> 0.05). P4 rats also showed increased skin hydration (38.6 ± 19.3 to 63.0 ± 4.47 (p <0.05)), and this was not found in other groups. The administration of topical black cumin seed extract 0.75% can inhibit decreased levels of collagen and increase skin tissue hydration.   

scholarly journals EVALUATION OF THE PROTECTIVE EFFECT OF NIGELLA SATIVA (BLACK CUMIN) OIL AGAINST VANCOMYCIN-INDUCED NEPHROTOXICITY IN RATS

2019 ◽  
pp. 332-336
Author(s):  
SARAH T OMRAN ◽  
JAWAD H AHMED
Keyword(s):  
Protective Effect ◽  
Normal Saline ◽  
Histopathological Examination ◽  
Nigella Sativa ◽  
Statistical Significance ◽  
Treated Group ◽  
Renal Tissue ◽  
Albino Rats ◽  
Black Cumin ◽  
Group I

Objective: The study was designed to investigate a possible protective effect of Nigella sativa (NS) against vancomycin (VAN)-induced nephrotoxicity in rats. Methods: Twenty-eight adult male Albino rats were randomly divided into four groups; seven rats in each. Group I (control): The animals were treated with normal saline (2 ml/kg/day) given orally and intraperitoneally (IP); Group II: VAN was given at a dose of 400 mg/kg/day for 7 days IP and normal saline orally; Group III: NS oil was given at a dose of 2 ml/kg/day for seven days orally and normal saline IP; and Group IV: VAN 400 mg/kg/day IP in combination with NS oil 2 ml/kg/day orally for 7 days. Twenty-four hours after the last dose, the animals were sacrificed, and serum was collected to estimate urea and creatinine. Then, both kidneys were excised, one for homogenate preparation to estimate renal tissue malondialdehyde (MDA), glutathione (GSH) and neutrophil gelatinase-associated lipocalin (NGAL) and the other for histopathological examination. Results: NS significantly decreased serum urea and creatinine compared to VAN treated group, p<0.001. NS significantly increased renal tissue GSH compared to VAN treated group p<0.001. NS lowered MDA and NGAL levels in the homogenate of renal tissues compared to their elevated levels in rats treated with VAN, but this did not achieve statistical significance. NS also ameliorated renal histopathological changes induced by VAN. Conclusion: NS has a protective effect against VAN-induced nephrotoxicity.

Optimisation of the Oil Extraction from Nigella sativa Seeds Using Response Surface Methodology

2017 ◽  
Vol 68 (2) ◽  
pp. 331-336
Author(s):  
Gabriela Isopencu ◽  
Mirela Marfa ◽  
Iuliana Jipa ◽  
Marta Stroescu ◽  
Anicuta Stoica Guzun ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Nigella Sativa ◽  
Polynomial Equation ◽  
Herbaceous Plant ◽  
Box Behnken Design ◽  
Pareto Analysis ◽  
Black Cumin ◽  
Mediterranean Countries ◽  
Experimental Values

Nigella sativa, also known as black cumin, an annual herbaceous plant growing especially in Mediterranean countries, has recently gained considerable interest not only for its use as spice and condiment but also for its healthy properties of the fixed and essential oil and its potential as a biofuel. Nigella sativa seeds fixed oil, due to its high content in linoleic acid followed by oleic and palmitic acid, could be beneficial to human health. The objective of this study is to determine the optimum conditions for the solvent extraction of Nigella sativa seeds fixed oil using a three-level, three-factor Box-Behnken design (BBD) under response surface methodology (RSM). The obtained experimental data, fitted by a second-order polynomial equation were analysed by Pareto analysis of variance (ANOVA). From a total of 10 coefficients of the statistical model only 5 are important. The obtained experimental values agreed with the predicted ones.

The protective effect of Nigella Sativa oil extract against neurotoxicity induced by Valproic acid

2017 ◽  
Vol 6 (9) ◽  
pp. 5474 ◽  
Author(s):  
Basant Mahmoud Morsy ◽  
Ghada Mohamed Safwat ◽  
Doaa Ahmed Hussein ◽  
Reem Mohamed Samy
Keyword(s):  
Valproic Acid ◽  
Nigella Sativa ◽  
Liver Lipid ◽  
Black Cumin ◽  
Enzymatic Antioxidant ◽  
Nigella Sativa Oil ◽  
Active Chemical ◽  
Oil Extract ◽  
Liver Lipid Peroxidation

Nigella sativa (NS), commonly known as black cumin, has been used for medicinal purposes. Traditionally the seeds and its oil are used in several diseases. The greatest part of the remedial properties of this plant is due to the presence of thymoquinone (TQ) which is a major active chemical component of the essential oil. The current study performed to evaluate the effect of Nigella sativa Oil (NSO) extract on the neurotoxic and hepatotoxic potentials from Valproic acid (VPA) administration. Also we summarize recent findings emphasizing the role of main neurotoxic and hepatotoxic markers and oxidative stress in study’s case. Neurotoxicity was induced by VPA at dose of (500 mg/kg b.wt) by gastric intubation daily for 30 day. These rats received NSO extract was given orally at dose of (0.5 ml/kg b.wt) daily for 30 days after VPA administration. The current results revealed that NSO extract treatment ameliorated significantly the elevated levels of the neurotoxic and hepatotoxic biomarkers which elevated as a result to VPA administration. Moreover, NSO extract treatment ameliorated the non-enzymatic antioxidant, brain and liver lipid peroxidation (LPO) and glutathione (GSH) concentration and the enzymatic antioxidant, brain and liver catalase(CAT) activity.

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