INVESTIGATION OF THE EFFECT OF NATURAL NIGERIAN CALCIUM BENTONITE ON HAEMATOLOGICAL PARAMETERS OF WISTAR ALBINO RATS

Despite Fe deficiency having been widely studied, the sequence of events in its development still remains unclear. The aim of the present study was to elucidate the effects of nutritional Fe-deficiency development on haematological parameters, Fe bioavailability and the enzymes involved in oxidative defence in recently weaned male Wistar albino rats. Control (C) and Fe-deficient (ID) groups were fed the AIN-93 G diet with a normal Fe level (45 mg/kg diet) or with a low Fe level (5 mg/kg diet), respectively, for 20, 30 or 40 d. At day 20 serum Fe, serum ferritin and the saturation of transferrin decreased drastically, decreasing further in the course of Fe-deficiency development for the saturation of transferrin. The development of Fe deficiency did not affect plasma thiobarbituric acid-reactive substance production, or catalase (CAT) and glutathione peroxidase (GPx) activities in erythrocyte cytosol. Fe deficiency diminished hepatic Fe content and CAT and GPx activities in hepatic cytosol only at day the 20. However, in spite of the minor Fe deposits in the brain of ID rats, the CAT and GPx activities in the brain cytosolic fraction did not differ in any of the studied periods v. control rats. These results show that brain is a tissue that does not seem to depend on Fe levels for the maintenance of antioxidant defence mechanisms in the course of nutritional Fe deficiency.

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Effect of Methanol Extract of Ricinus communis (RC) Seeds on Blood Glucose Levels, Antioxidant Enzymes and Haematological Parameters of Alloxan Induced Male Wistar Albino Rats

Research Journal of Medicinal Plant ◽

10.3923/rjmp.2016.140.149 ◽

2016 ◽

Vol 10 (2) ◽

pp. 140-149 ◽

Cited By ~ 1

Author(s):

Uroko Robert Ikechukwu ◽

E. Joshua Parker ◽

Nweje-Anyalowu Paul Chukwuemek ◽

O.F.C. Nwodo

Keyword(s):

Antioxidant Enzymes ◽

Blood Glucose ◽

Methanol Extract ◽

Ricinus Communis ◽

Haematological Parameters ◽

Albino Rats ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Wistar Albino Rats

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Hormonal profile and haematological parameters of Wistar albino rats treated with methanolic and aqueous leaf extracts of Schleichera oleosa

10.21203/rs.3.rs-199227/v1 ◽

2021 ◽

Author(s):

Sophy Jose ◽

M. P. Sinha

Keyword(s):

Aqueous Extract ◽

Low Dose ◽

Methanolic Extract ◽

Haematological Parameters ◽

Albino Rats ◽

High Dose ◽

Corpuscular Haemoglobin ◽

Hormonal Profile ◽

Schleichera Oleosa ◽

Wistar Albino Rats

Abstract Background: The present study aims to study the Hormonal profile and haematological parameters of Wistar albino rats treated with methanolic and aqueous extracts of Schleichera oleosa.Result: The amount of RBC was found to be 9.6±0.057106/mm3, 9.7±0.0125, 106/ 9.5±0.003, 106/mm3 9.5±0.012 106/mm3 and 9.5±0.023 106/mm3. The amount of WBC was found to be 13.5±0.058 103/mm3,13.6±0.0113 103/mm3,13.7±0.021 103/mm3, 9.5±0.012 103/mm3 and 9.5±0.023 103/mm3. Platelets, 396±0.577 103/mm3, 395±0.03 103/mm3 , 397±0.042103/mm3 , 398±0.020103/mm3, and 397±0.011103/mm3 for controlled group, low dose of methanolic extract, high dose of methanolic extract, low dose of aqueous extract and high dose of aqueous extract respectively. The packed cell volume, haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin, corpuscular haemoglobin concentration and amount of neutrophils, Eosinophils, Lymphocytes, Basophils and Monocytes were measured and no significant change was noticed. The amount of testosterone showed significant increase from 0.75± 0.024 pg/ mL (control) 2.96± 0.088 pg/ mL, 3.9± 0.14pg / mL, 1.85± 0.076 pg/ mL and 2.85± 0.074 pg/ mL.Conclusions: The study suggests that the prolonged treatment of Wistar rats with both low dose and high dose of methanolic as well as the aqueous extract of Schleichera oleosa is not toxic and they increased the amount of testosterone in the blood which is indicative of their testosterone enhancement.

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