UJI KANDUNGAN VITAMIN A TANAMAN WORTEL (Daucus Corata L.) DI DESA NGABAB KABUPATEN MALANG

Carrot plant (Daucus carota L.) is a type of vegetable that contains lots of vitamin A and is very much needed by the body to help regulate or metabolic processes in the body. The research design used in this study was experimental, the population and sample were carrots from Ngabab village aged 3-3.5 months, using purposive sampling technique. The results of the qualitative analysis showed that there were orange spots with an Rf value of 0.64 in the carrot extract and the beta-carotene comparison standard. The results of the quantitative analysis showed that the average level of vitamin A in carrots was 49.7% with an absorbance of 0.649. Keywords: Visible Spectrophotometry, Vitamin A, Carrot.

Seed Priming Boost Adaptation in Pea Plants under Drought Stress

In the present investigation, we study the effect of Bacillus thuringiensis MH161336 (106–8 CFU/cm3), silicon (25 mL L−1), and carrot extract (75 mL L−1) as seed primers, individually or in combination, on morphological, physio-biochemical and yield components of drought-stressed pea plants (Master B) during 2019/2020 and 2020/2021 seasons. Our results indicated that drought causes a remarkable reduction in plant height, leaf area, number of leaves per plant, and number of flowers per plant in stressed pea plants during two seasons. Likewise, number of pods, pod length, seeds weight of 10 dried plants, and dry weight of 100 seeds were decreased significantly in drought-stressed pea plants. Nevertheless, seed priming with the individual treatments or in combination boosted the morphological, physio-biochemical, and yield characters of pea plants. The best results were obtained with the Bacillus thuringiensis + carrot extract treatment, which led to a remarkable increase in the number of leaves per plant, leaf area, plant height, and number of flowers per plant in stressed pea plants in both seasons. Moreover, pod length, number of seeds per pod, seeds weight of 10 dried plants, and dry weight of 100 seeds were significantly increased as well. Bacillus thuringiensis + carrot extract treatment led to improved biochemical and physiological characters, such as relative water content, chlorophyll a, chlorophyll b, regulated the up-regulation of antioxidant enzymes, increased seed yield, and decreased lipid peroxidation and reactive oxygen species, mainly superoxide and hydrogen peroxide, in drought-stressed pea plants.

EKSTRAK LABU SIAM DAN WORTEL TERHADAP TEKANAN DARAH PADA LANSIA YANG MENDERITA HIPERTENSI DI SASANA KARTINI KELURAHAN TOSAREN

Hypertension is a condition of vasoconstriction of blood vessels caused by several factors such as age, gender and irregular eating patterns. The Chayote and carrot extract as non-pharmacological therapy can lower blood pressure because they contain high potassium. The purpose of this study was to determine the effect of the use of chayote and carrot extracts on blood pressure in the elderly who suffer from hypertension in Sasana Kartini, Tosaren Village. The research design used is Pre-Experimental Designs, the population is all elderly people with hypertension, which are 52 respondents, the number of samples that meet the inclusive criteria, as many as 19 respondents using purposive sampling. The independent variable in this study was the administration of chayote extract and the dependent variable was systolic and diastolic blood pressure, the instruments used for data collection research were sphygmomanometer and observation sheets. While the data processing uses the Paired Sample T-Test.

Application of vigor indexes to evaluate the cold tolerance in rice seeds germination conditioned in plant extract

Rice is a crop that presents sensitivity to cold, especially in the germination phase, which leads to high economic losses. Alternative management forms are essential to increase tolerance to low temperatures, and seed priming represents a promising tool. The objective of this study was to investigate the priming effect of the aqueous extract of carrot roots on rice seeds to increase tolerance to low temperatures during germination. Seeds from cultivars BRS Querência (cold-susceptible) and Brilhante (cold-tolerant) were soaked for 24 h in concentrations of 0, 25, 50, and 100% carrot extract, sown on germitest paper and conditioned in BOD for 21 days at 15 °C. As a control, the seeds soaked in water were also germinated at 25 °C. They were evaluated for germination, first germination count, and germination speed index to calculate the stress indices: tolerance index, susceptibility index, and harmonic mean. They were also evaluated for the length and dry mass of shoot and root. The results showed that the rice seeds conditioning in carrot extract effectively reduces the damage caused by cold, significantly increasing the germination speed and the percentage of final germination and the growth evaluations, more expressive at 100% concentration. The stress indexes are efficient in estimating the tolerance of the cultivars and the effect of the different conditions in low-temperature conditions, highlighting the superiority of the Brilhante cultivar.

Mitigating dietary aflatoxins in Wistar rat using selected phyto-antioxidant sources

This study was conducted to investigate the mitigation of dietary aflatoxin in Wistar rats using extract from selected phyto-antioxidant sources. A total of one hundred and twenty Wistar rats weighing between 180-190g (80 females and 40 males) at six weeks old were allotted to eight treatments with 15 rats per treatment (10 females and 5 males), in a completely randomized design. The treatments were Treatment 1 (Normal diet + no extract and no aflatoxin), Treatment 2 (Aflatoxin contaminated diet + no extract), Treatment 3 (Aflatoxin contaminated diet + 100mg/kg BW Carrot extract), Treatment 4 (Aflatoxin contaminated diet + 200mg/kg BW Carrot extract), Treatment 5 (Aflatoxin contaminated diet + 100mg/kg BW Ginger extract), Treatment 6 (Aflatoxin contaminated diet + 200mg/kg BW Ginger extract), Treatment 7 (Aflatoxin contaminated diet +100mg/kg BW Garlic extract), Treatment 8 (Aflatoxin contaminated diet+ 200mg/kg BW Garlic extract). Body weight gain and feed conversion ratio of Wister rats (T8) administered 200 mg/kg BW was significant (p<0.05), with higher weight gain in male (206.00g) and female (199.70g). However, eviscerated and organs weights of both male and female Wistar rats was similar (p>0.05) across the treatments. Haematological and serum biochemical indices among the treatments was not significant (p>0.05), except for the globulin in male Wistar rats that differed significantly (p<0.05) with the value (5.00g/dL) being higher in T7. In conclusion, 200mg/kg body weight of garlic extract improved growth rate of Wistar rats, without any deleterious effect on haematological and serum biochemical parameters. Therefore, 200mg/kg body weight garlic extract mitigated the adverse effect of aflatoxin contaminated feed in Wistar rats. Cette étude a été menée pour étudier l'atténuation de l'aflatoxine diététique chez les rats de 'Wistar' tout en employant l'extrait des sources phyto-antioxydantes choisies. Un total de cent vingt rats Wistar pesant entre 180 et 190 g (80 femelles et 40 mâles) à l'âge de six semaines ont été attribués à huit traitements avec 15 rats par traitement (10 femelles et 5 mâles), dans une conception complètement randomisée. Les traitements étaient le traitement 1 (régime normal + aucun extrait et aucune aflatoxine), traitement 2 (régime contaminé par l'aflatoxine + aucun extrait), traitement 3 (régime contaminé par l'aflatoxine + extrait de carotte BW de 100mg/kg), traitement 4 (régime contaminé par l'aflatoxine + extrait de carotte BW de 200mg/kg), traitement 5 (Aflatox alimentation contaminée par l'aflatoxine + extrait de gingembre BW 100mg/kg), Traitement 6 (régime contaminé à l'aflatoxine + extrait de gingembre BW 200mg/kg), Traitement 7 (régime contaminé par l'aflatoxine +100mg/kg extrait d'ail BW), Traitement 8 (régime contaminé à l'aflatoxine+ 200mg/kg extrait d'ail BW). Le gain de poids corporel et le rapport de conversion des aliments pour animaux des rats wisters (T8) administrés 200 mg/kg BW étaient significatifs (p<0.05), avec un gain de poids plus élevé chez les rats Wistar mâles (206.00 g) et femelles (199.70 g). Cependant, les poids éviscérés et organes des rats Wistar mâles et femelles étaient similaires (p>0.05) à travers les traitements. Les indices biochimiques hématologiques et sériques parmi les traitements n'étaient pas significatifs (p>0.05), à l'exception de la globuline chez les rats wistar mâles qui différait considérablement (p<0.05) avec la valeur (5.00 g/dL) étant plus élevée dans T7. En conclusion, le poids corporel de 200mg/kg de l'extrait d'ail a amélioré le taux de croissance des rats de Wistar, sans n'importe quel effet délétère sur les paramètres biochimiques hématologiques et sériques. Par conséquent, l'extrait d'ail de poids corporel de 200mg/kg a atténué l'effet défavorable de l'alimentation contaminée d'aflatoxin chez les rats de Wistar.

Penambahan Level Ekstrak Wortel (Daucus carota) pada Pengencer Andromed Dalam Mempertahankan Kualitas Spermatozoa Sapi Bali pada Suhu 5 OC

A good diluents must have the ability to reduce the rate of decline in the quality of spermatozoa, by choosing the right diluents that is able to maintain the quality of spermatozoa. The addition of antioxidants is believed to be able to maintain spermatozoa life longer, one of which is the addition of carrot extract. The purpose of this study was to maintain the quality of Bali cattle spermatozoa stored at 5 0C by utilizing the quality of bali cattle spermatozoa because they contain antioxidants. This study used a completely randomized design (CRD) consisting of 5 treatments and 5 replications, namely: P0 = cement + andromed, P1 = cement + andromed + 5 % carrot extract, P2 = cement + andromed + 10 % carrot extract, P3 = cement + andromed + 15 % carrot extract, P4 = cement + andromed + 20 % carrot extract. The variables measured were the quality of the spermatozoa microscopically, namely the motility and viability of the spermatozoa. The result showed that the increase in the level of carrot extract in andromed diluent in maintaining spermatozoa motility at 5 0C was not significant (P>0,05), meaning that each treatment had the same effect in maintaining spermatozoa motility. The percentage of motility in all treatments is feasible to be applied to the IB program because is still shows a value of more than 40 %.