scholarly journals Root Phenology and Biochemical Changes in Rice Genotypes under Drought Stress

Author(s):  
S. Behera ◽  
R.K. Rout ◽  
B. Sinha ◽  
A. Padhiary ◽  
A. Nayak ◽  
...  
Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mahmoud M. Gaballah ◽  
Azza M. Metwally ◽  
Milan Skalicky ◽  
Mohamed M. Hassan ◽  
Marian Brestic ◽  
...  

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.


2017 ◽  
Vol 40 (4) ◽  
pp. 841-851 ◽  
Author(s):  
Xiaomin Chen ◽  
Lingling Qiu ◽  
Haipeng Guo ◽  
Yang Wang ◽  
Huwei Yuan ◽  
...  

2020 ◽  
Vol 10 (13) ◽  
pp. 4471
Author(s):  
Didar Rahim ◽  
Petr Kalousek ◽  
Nawroz Tahir ◽  
Tomáš Vyhnánek ◽  
Petr Tarkowski ◽  
...  

Rice (Oryza sativa L.) is productively affected by different environmental factors, including biotic and abiotic stress. The objectives of this research were to evaluate the genetic distinction among Kurdish rice genotypes using the simple sequence repeats (SSRs) molecular markers and to perform in vitro tests to characterize the drought tolerance of six local rice genotypes. The polymorphic information content (PIC) varied from 0.38 to 0.84 with an average of 0.56. The genetic distance ranged from 0.33 to 0.88. Drought stress had a significant impact (p ≤ 0.05) on callus growth parameters. Enzymatic antioxidant systems were predicted and exhibited a significant variation. The findings revealed that proline levels increase in proportion to polyethylene glycol (PEG) concentrations. Kalar and Gwll Swr genotypes showed the worst performances in phenotypic and biochemical traits, while Choman and Shawre exhibited the best phenotypic and biochemical performances. A positive and substantial relationship between callus fresh weight (CFW) and callus dry weight (CDW) was found under stressful and optimized conditions. Callus induction (CI) was positively and significantly associated with the catalase activity (CAT) in all stressed treatments. Based on the results for callus growth and the biochemical parameters under stress conditions, a remarkable genotype distinction, based on the tolerance reaction, was noted as follows: PEG resistant > susceptible, Choman > Shawre > White Bazyan > Red Bazyan > Gwll Swr > Kalar. The CI and CAT characteristics were considered as reliable predictors of drought tolerance in rice genotypes.


2018 ◽  
Vol 16 (1) ◽  
pp. 44-54 ◽  
Author(s):  
M M Islam ◽  
E Kayesh ◽  
E Zaman ◽  
T A Urmi ◽  
M M Haque

Drought stress is a major constraint to the production and yield stability of crops. Rice (Oryza sativa L.) is considered as a drought-sensitive crop species. Within this species, there are considerable varietal differences in sensitivity to this environmental stress. An experiment was conducted at the laboratory of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during April to September 2016 to evaluate 100 rice genotypes for drought tolerance during germination and early seedling growth stage. The genotypes were tested against five levels of drought stress imposed by Polyethylene glycol 6000 (PEG-6000) @ 0, 5, 10, 15 and 20%. The experiment was laid out in a complete randomized design with four replications. The results showed that with increasing water stress, germination in all the genotypes decreased from 95.8% in control to 6.6 % in highest stress (20% PEG) level. Seedling height and dry weight also decreased in all rice genotypes with the increase in water stress level. Based on Standard Evaluation System (SES),18 genotypes were selected primarily. Among the 18 genotypes, Ratoil and Chinisakkar showed higher germination index, relative seedling height and relative seedling dry weight than the check drought tolerant BRRI dhan43 at 20% PEG. Beside these, performance of Kumridhan, Pusur and Somondori was also well at this level compared to BRRI dhan43. The genotypes Ratoil, Chinisakkar, Kumridhan, Pusur and Somondori showed the best performance under drought condition. The Agriculturists 2018; 16(1) 44-54


2013 ◽  
Vol 1 (1) ◽  
pp. 67
Author(s):  
Feni Shintarika ◽  
Faiza Chairani Suwarno ◽  
. Suwarno

<p style="text-align: justify;">The objectives of the study were to evaluate seed storability and vigor to drought of rice genotypes from different ecologies and correlation among the characters. The experiments were conducted in the Seed Science and Technology Laboratory, Bogor Agricultural University from April to August 2011. Duration of rapid aging using ethanol vapour for testing seed storability were 87.6 and 155.4 minutes for upland and lowland rice seeds respectively. The rolled paper methods with Polyethylen Glycol (PEG 6000) at -2.0 bar was used for testing seed vigor to drought. Rice seed of 40 genotype consisting of 20 upland and 20 lowland were evaluated using Randomized Block Design with three replications. There were no correlation between seed storability and vigor to drought on all genotypes, and no similiarity of those characters on the best five genotypes. Genotypes with the highest storability were B12154D-MR-22-8 for upland rice and Aek Sibundong for lowland. Genotypes with the highest vigor to drought were B12165D-MR-8-1 and B12161D-MR-1-4-2 for upland rice, B12672-MR-19-2-PN-1-3 and Aek Sibundong for lowland.</p><p>Keywords : drought stress, Rapid aging</p>


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